Job ID = 12264760 SRX = SRX3029125 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 54995702 spots for SRR5860425/SRR5860425.sra Written 54995702 spots for SRR5860425/SRR5860425.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265624 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:01:37 54995702 reads; of these: 54995702 (100.00%) were paired; of these: 25095744 (45.63%) aligned concordantly 0 times 26026662 (47.32%) aligned concordantly exactly 1 time 3873296 (7.04%) aligned concordantly >1 times ---- 25095744 pairs aligned concordantly 0 times; of these: 6796193 (27.08%) aligned discordantly 1 time ---- 18299551 pairs aligned 0 times concordantly or discordantly; of these: 36599102 mates make up the pairs; of these: 33424123 (91.32%) aligned 0 times 1512119 (4.13%) aligned exactly 1 time 1662860 (4.54%) aligned >1 times 69.61% overall alignment rate Time searching: 01:01:37 Overall time: 01:01:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 8325744 / 36224245 = 0.2298 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:01:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:01:52: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:01:52: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:01:58: 1000000 INFO @ Sat, 03 Apr 2021 08:02:05: 2000000 INFO @ Sat, 03 Apr 2021 08:02:11: 3000000 INFO @ Sat, 03 Apr 2021 08:02:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:02:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:02:22: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:02:22: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:02:24: 5000000 INFO @ Sat, 03 Apr 2021 08:02:29: 1000000 INFO @ Sat, 03 Apr 2021 08:02:31: 6000000 INFO @ Sat, 03 Apr 2021 08:02:36: 2000000 INFO @ Sat, 03 Apr 2021 08:02:39: 7000000 INFO @ Sat, 03 Apr 2021 08:02:44: 3000000 INFO @ Sat, 03 Apr 2021 08:02:46: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:02:52: 4000000 INFO @ Sat, 03 Apr 2021 08:02:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:02:52: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:02:52: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:02:54: 9000000 INFO @ Sat, 03 Apr 2021 08:02:59: 5000000 INFO @ Sat, 03 Apr 2021 08:03:00: 1000000 INFO @ Sat, 03 Apr 2021 08:03:01: 10000000 INFO @ Sat, 03 Apr 2021 08:03:07: 2000000 INFO @ Sat, 03 Apr 2021 08:03:07: 6000000 INFO @ Sat, 03 Apr 2021 08:03:09: 11000000 INFO @ Sat, 03 Apr 2021 08:03:15: 3000000 INFO @ Sat, 03 Apr 2021 08:03:15: 7000000 INFO @ Sat, 03 Apr 2021 08:03:17: 12000000 INFO @ Sat, 03 Apr 2021 08:03:23: 8000000 INFO @ Sat, 03 Apr 2021 08:03:23: 4000000 INFO @ Sat, 03 Apr 2021 08:03:25: 13000000 INFO @ Sat, 03 Apr 2021 08:03:31: 5000000 INFO @ Sat, 03 Apr 2021 08:03:31: 9000000 INFO @ Sat, 03 Apr 2021 08:03:33: 14000000 INFO @ Sat, 03 Apr 2021 08:03:39: 10000000 INFO @ Sat, 03 Apr 2021 08:03:39: 6000000 INFO @ Sat, 03 Apr 2021 08:03:41: 15000000 INFO @ Sat, 03 Apr 2021 08:03:47: 11000000 INFO @ Sat, 03 Apr 2021 08:03:47: 7000000 INFO @ Sat, 03 Apr 2021 08:03:49: 16000000 INFO @ Sat, 03 Apr 2021 08:03:55: 8000000 INFO @ Sat, 03 Apr 2021 08:03:55: 12000000 INFO @ Sat, 03 Apr 2021 08:03:57: 17000000 INFO @ Sat, 03 Apr 2021 08:04:03: 9000000 INFO @ Sat, 03 Apr 2021 08:04:03: 13000000 INFO @ Sat, 03 Apr 2021 08:04:05: 18000000 INFO @ Sat, 03 Apr 2021 08:04:10: 10000000 INFO @ Sat, 03 Apr 2021 08:04:10: 14000000 INFO @ Sat, 03 Apr 2021 08:04:13: 19000000 INFO @ Sat, 03 Apr 2021 08:04:18: 15000000 INFO @ Sat, 03 Apr 2021 08:04:18: 11000000 INFO @ Sat, 03 Apr 2021 08:04:21: 20000000 INFO @ Sat, 03 Apr 2021 08:04:26: 16000000 INFO @ Sat, 03 Apr 2021 08:04:27: 12000000 INFO @ Sat, 03 Apr 2021 08:04:29: 21000000 INFO @ Sat, 03 Apr 2021 08:04:34: 17000000 INFO @ Sat, 03 Apr 2021 08:04:35: 13000000 INFO @ Sat, 03 Apr 2021 08:04:37: 22000000 INFO @ Sat, 03 Apr 2021 08:04:42: 18000000 INFO @ Sat, 03 Apr 2021 08:04:42: 14000000 INFO @ Sat, 03 Apr 2021 08:04:45: 23000000 INFO @ Sat, 03 Apr 2021 08:04:50: 19000000 INFO @ Sat, 03 Apr 2021 08:04:50: 15000000 INFO @ Sat, 03 Apr 2021 08:04:53: 24000000 INFO @ Sat, 03 Apr 2021 08:04:58: 16000000 INFO @ Sat, 03 Apr 2021 08:04:58: 20000000 INFO @ Sat, 03 Apr 2021 08:05:01: 25000000 INFO @ Sat, 03 Apr 2021 08:05:06: 17000000 INFO @ Sat, 03 Apr 2021 08:05:06: 21000000 INFO @ Sat, 03 Apr 2021 08:05:09: 26000000 INFO @ Sat, 03 Apr 2021 08:05:14: 18000000 INFO @ Sat, 03 Apr 2021 08:05:14: 22000000 INFO @ Sat, 03 Apr 2021 08:05:17: 27000000 INFO @ Sat, 03 Apr 2021 08:05:22: 19000000 INFO @ Sat, 03 Apr 2021 08:05:22: 23000000 INFO @ Sat, 03 Apr 2021 08:05:25: 28000000 INFO @ Sat, 03 Apr 2021 08:05:30: 20000000 INFO @ Sat, 03 Apr 2021 08:05:30: 24000000 INFO @ Sat, 03 Apr 2021 08:05:33: 29000000 INFO @ Sat, 03 Apr 2021 08:05:38: 21000000 INFO @ Sat, 03 Apr 2021 08:05:38: 25000000 INFO @ Sat, 03 Apr 2021 08:05:41: 30000000 INFO @ Sat, 03 Apr 2021 08:05:46: 22000000 INFO @ Sat, 03 Apr 2021 08:05:46: 26000000 INFO @ Sat, 03 Apr 2021 08:05:49: 31000000 INFO @ Sat, 03 Apr 2021 08:05:54: 23000000 INFO @ Sat, 03 Apr 2021 08:05:54: 27000000 INFO @ Sat, 03 Apr 2021 08:05:57: 32000000 INFO @ Sat, 03 Apr 2021 08:06:02: 24000000 INFO @ Sat, 03 Apr 2021 08:06:03: 28000000 INFO @ Sat, 03 Apr 2021 08:06:05: 33000000 INFO @ Sat, 03 Apr 2021 08:06:10: 25000000 INFO @ Sat, 03 Apr 2021 08:06:11: 29000000 INFO @ Sat, 03 Apr 2021 08:06:13: 34000000 INFO @ Sat, 03 Apr 2021 08:06:18: 26000000 INFO @ Sat, 03 Apr 2021 08:06:19: 30000000 INFO @ Sat, 03 Apr 2021 08:06:20: 35000000 INFO @ Sat, 03 Apr 2021 08:06:26: 27000000 INFO @ Sat, 03 Apr 2021 08:06:26: 31000000 INFO @ Sat, 03 Apr 2021 08:06:28: 36000000 INFO @ Sat, 03 Apr 2021 08:06:34: 32000000 INFO @ Sat, 03 Apr 2021 08:06:34: 28000000 INFO @ Sat, 03 Apr 2021 08:06:36: 37000000 INFO @ Sat, 03 Apr 2021 08:06:42: 33000000 INFO @ Sat, 03 Apr 2021 08:06:43: 29000000 INFO @ Sat, 03 Apr 2021 08:06:44: 38000000 INFO @ Sat, 03 Apr 2021 08:06:50: 34000000 INFO @ Sat, 03 Apr 2021 08:06:50: 30000000 INFO @ Sat, 03 Apr 2021 08:06:51: 39000000 INFO @ Sat, 03 Apr 2021 08:06:57: 35000000 INFO @ Sat, 03 Apr 2021 08:06:58: 31000000 INFO @ Sat, 03 Apr 2021 08:06:59: 40000000 INFO @ Sat, 03 Apr 2021 08:07:05: 36000000 INFO @ Sat, 03 Apr 2021 08:07:06: 32000000 INFO @ Sat, 03 Apr 2021 08:07:06: 41000000 INFO @ Sat, 03 Apr 2021 08:07:13: 37000000 INFO @ Sat, 03 Apr 2021 08:07:14: 42000000 INFO @ Sat, 03 Apr 2021 08:07:14: 33000000 INFO @ Sat, 03 Apr 2021 08:07:21: 38000000 INFO @ Sat, 03 Apr 2021 08:07:21: 43000000 INFO @ Sat, 03 Apr 2021 08:07:22: 34000000 INFO @ Sat, 03 Apr 2021 08:07:29: 39000000 INFO @ Sat, 03 Apr 2021 08:07:29: 44000000 INFO @ Sat, 03 Apr 2021 08:07:29: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:07:36: 40000000 INFO @ Sat, 03 Apr 2021 08:07:37: 45000000 INFO @ Sat, 03 Apr 2021 08:07:37: 36000000 INFO @ Sat, 03 Apr 2021 08:07:44: 41000000 INFO @ Sat, 03 Apr 2021 08:07:45: 46000000 INFO @ Sat, 03 Apr 2021 08:07:45: 37000000 INFO @ Sat, 03 Apr 2021 08:07:51: 42000000 INFO @ Sat, 03 Apr 2021 08:07:52: 47000000 INFO @ Sat, 03 Apr 2021 08:07:53: 38000000 INFO @ Sat, 03 Apr 2021 08:07:59: 43000000 INFO @ Sat, 03 Apr 2021 08:07:59: 48000000 INFO @ Sat, 03 Apr 2021 08:08:00: 39000000 INFO @ Sat, 03 Apr 2021 08:08:06: 44000000 INFO @ Sat, 03 Apr 2021 08:08:07: 49000000 INFO @ Sat, 03 Apr 2021 08:08:08: 40000000 INFO @ Sat, 03 Apr 2021 08:08:14: 45000000 INFO @ Sat, 03 Apr 2021 08:08:14: 50000000 INFO @ Sat, 03 Apr 2021 08:08:15: 41000000 INFO @ Sat, 03 Apr 2021 08:08:21: 46000000 INFO @ Sat, 03 Apr 2021 08:08:22: 51000000 INFO @ Sat, 03 Apr 2021 08:08:22: 42000000 INFO @ Sat, 03 Apr 2021 08:08:29: 47000000 INFO @ Sat, 03 Apr 2021 08:08:30: 52000000 INFO @ Sat, 03 Apr 2021 08:08:30: 43000000 INFO @ Sat, 03 Apr 2021 08:08:36: 48000000 INFO @ Sat, 03 Apr 2021 08:08:37: 53000000 INFO @ Sat, 03 Apr 2021 08:08:37: 44000000 INFO @ Sat, 03 Apr 2021 08:08:43: 49000000 INFO @ Sat, 03 Apr 2021 08:08:45: 45000000 INFO @ Sat, 03 Apr 2021 08:08:45: 54000000 INFO @ Sat, 03 Apr 2021 08:08:51: 50000000 INFO @ Sat, 03 Apr 2021 08:08:52: 46000000 INFO @ Sat, 03 Apr 2021 08:08:53: 55000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:08:58: 51000000 INFO @ Sat, 03 Apr 2021 08:09:00: 47000000 INFO @ Sat, 03 Apr 2021 08:09:00: 56000000 INFO @ Sat, 03 Apr 2021 08:09:06: 52000000 INFO @ Sat, 03 Apr 2021 08:09:08: 48000000 INFO @ Sat, 03 Apr 2021 08:09:08: 57000000 INFO @ Sat, 03 Apr 2021 08:09:14: 53000000 INFO @ Sat, 03 Apr 2021 08:09:16: 49000000 INFO @ Sat, 03 Apr 2021 08:09:17: 58000000 INFO @ Sat, 03 Apr 2021 08:09:22: 54000000 INFO @ Sat, 03 Apr 2021 08:09:23: 50000000 INFO @ Sat, 03 Apr 2021 08:09:25: 59000000 INFO @ Sat, 03 Apr 2021 08:09:30: 55000000 INFO @ Sat, 03 Apr 2021 08:09:31: 51000000 INFO @ Sat, 03 Apr 2021 08:09:32: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:09:32: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:09:32: #1 total tags in treatment: 23341142 INFO @ Sat, 03 Apr 2021 08:09:32: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:09:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:09:32: #1 tags after filtering in treatment: 16014411 INFO @ Sat, 03 Apr 2021 08:09:32: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 08:09:32: #1 finished! INFO @ Sat, 03 Apr 2021 08:09:32: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:09:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:09:34: #2 number of paired peaks: 202 WARNING @ Sat, 03 Apr 2021 08:09:34: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Sat, 03 Apr 2021 08:09:34: start model_add_line... INFO @ Sat, 03 Apr 2021 08:09:34: start X-correlation... INFO @ Sat, 03 Apr 2021 08:09:34: end of X-cor INFO @ Sat, 03 Apr 2021 08:09:34: #2 finished! INFO @ Sat, 03 Apr 2021 08:09:34: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 08:09:34: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 08:09:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.05_model.r WARNING @ Sat, 03 Apr 2021 08:09:34: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:09:34: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 08:09:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:09:34: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:09:34: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:09:38: 56000000 INFO @ Sat, 03 Apr 2021 08:09:38: 52000000 INFO @ Sat, 03 Apr 2021 08:09:45: 57000000 INFO @ Sat, 03 Apr 2021 08:09:46: 53000000 INFO @ Sat, 03 Apr 2021 08:09:52: 58000000 INFO @ Sat, 03 Apr 2021 08:09:53: 54000000 INFO @ Sat, 03 Apr 2021 08:09:59: 59000000 INFO @ Sat, 03 Apr 2021 08:10:00: 55000000 INFO @ Sat, 03 Apr 2021 08:10:03: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:10:06: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:10:06: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:10:06: #1 total tags in treatment: 23341142 INFO @ Sat, 03 Apr 2021 08:10:06: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:10:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:10:06: #1 tags after filtering in treatment: 16014411 INFO @ Sat, 03 Apr 2021 08:10:06: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 08:10:06: #1 finished! INFO @ Sat, 03 Apr 2021 08:10:06: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:10:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:10:07: 56000000 INFO @ Sat, 03 Apr 2021 08:10:08: #2 number of paired peaks: 202 WARNING @ Sat, 03 Apr 2021 08:10:08: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Sat, 03 Apr 2021 08:10:08: start model_add_line... INFO @ Sat, 03 Apr 2021 08:10:08: start X-correlation... INFO @ Sat, 03 Apr 2021 08:10:08: end of X-cor INFO @ Sat, 03 Apr 2021 08:10:08: #2 finished! INFO @ Sat, 03 Apr 2021 08:10:08: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 08:10:08: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 08:10:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.10_model.r WARNING @ Sat, 03 Apr 2021 08:10:08: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:10:08: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 08:10:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:10:08: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:10:08: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:10:14: 57000000 INFO @ Sat, 03 Apr 2021 08:10:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:10:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:10:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.05_summits.bed INFO @ Sat, 03 Apr 2021 08:10:19: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (10090 records, 4 fields): 25 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:10:21: 58000000 INFO @ Sat, 03 Apr 2021 08:10:27: 59000000 INFO @ Sat, 03 Apr 2021 08:10:33: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:10:33: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:10:33: #1 total tags in treatment: 23341142 INFO @ Sat, 03 Apr 2021 08:10:33: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:10:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:10:33: #1 tags after filtering in treatment: 16014411 INFO @ Sat, 03 Apr 2021 08:10:33: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 08:10:33: #1 finished! INFO @ Sat, 03 Apr 2021 08:10:33: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:10:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:10:34: #2 number of paired peaks: 202 WARNING @ Sat, 03 Apr 2021 08:10:34: Fewer paired peaks (202) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 202 pairs to build model! INFO @ Sat, 03 Apr 2021 08:10:34: start model_add_line... INFO @ Sat, 03 Apr 2021 08:10:34: start X-correlation... INFO @ Sat, 03 Apr 2021 08:10:35: end of X-cor INFO @ Sat, 03 Apr 2021 08:10:35: #2 finished! INFO @ Sat, 03 Apr 2021 08:10:35: #2 predicted fragment length is 94 bps INFO @ Sat, 03 Apr 2021 08:10:35: #2 alternative fragment length(s) may be 4,94 bps INFO @ Sat, 03 Apr 2021 08:10:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.20_model.r WARNING @ Sat, 03 Apr 2021 08:10:35: #2 Since the d (94) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:10:35: #2 You may need to consider one of the other alternative d(s): 4,94 WARNING @ Sat, 03 Apr 2021 08:10:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:10:35: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:10:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:10:37: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:10:52: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:10:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:10:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.10_summits.bed INFO @ Sat, 03 Apr 2021 08:10:52: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (5924 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:11:03: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:11:18: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:11:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:11:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029125/SRX3029125.20_summits.bed INFO @ Sat, 03 Apr 2021 08:11:18: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (2350 records, 4 fields): 4 millis CompletedMACS2peakCalling