Job ID = 12264759 SRX = SRX3029124 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 61195528 spots for SRR5860424/SRR5860424.sra Written 61195528 spots for SRR5860424/SRR5860424.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265653 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:10:37 61195528 reads; of these: 61195528 (100.00%) were paired; of these: 24559480 (40.13%) aligned concordantly 0 times 31828361 (52.01%) aligned concordantly exactly 1 time 4807687 (7.86%) aligned concordantly >1 times ---- 24559480 pairs aligned concordantly 0 times; of these: 7660625 (31.19%) aligned discordantly 1 time ---- 16898855 pairs aligned 0 times concordantly or discordantly; of these: 33797710 mates make up the pairs; of these: 30231309 (89.45%) aligned 0 times 1621233 (4.80%) aligned exactly 1 time 1945168 (5.76%) aligned >1 times 75.30% overall alignment rate Time searching: 01:10:37 Overall time: 01:10:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 11716893 / 43729797 = 0.2679 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:17:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:17:53: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:17:53: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:18:00: 1000000 INFO @ Sat, 03 Apr 2021 08:18:06: 2000000 INFO @ Sat, 03 Apr 2021 08:18:12: 3000000 INFO @ Sat, 03 Apr 2021 08:18:18: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:18:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:18:23: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:18:23: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:18:24: 5000000 INFO @ Sat, 03 Apr 2021 08:18:29: 1000000 INFO @ Sat, 03 Apr 2021 08:18:30: 6000000 INFO @ Sat, 03 Apr 2021 08:18:36: 2000000 INFO @ Sat, 03 Apr 2021 08:18:36: 7000000 INFO @ Sat, 03 Apr 2021 08:18:42: 8000000 INFO @ Sat, 03 Apr 2021 08:18:42: 3000000 INFO @ Sat, 03 Apr 2021 08:18:48: 9000000 INFO @ Sat, 03 Apr 2021 08:18:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:18:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:18:53: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:18:53: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:18:54: 10000000 INFO @ Sat, 03 Apr 2021 08:18:56: 5000000 INFO @ Sat, 03 Apr 2021 08:19:00: 1000000 INFO @ Sat, 03 Apr 2021 08:19:01: 11000000 INFO @ Sat, 03 Apr 2021 08:19:03: 6000000 INFO @ Sat, 03 Apr 2021 08:19:07: 12000000 INFO @ Sat, 03 Apr 2021 08:19:08: 2000000 INFO @ Sat, 03 Apr 2021 08:19:10: 7000000 INFO @ Sat, 03 Apr 2021 08:19:14: 13000000 INFO @ Sat, 03 Apr 2021 08:19:15: 3000000 INFO @ Sat, 03 Apr 2021 08:19:17: 8000000 INFO @ Sat, 03 Apr 2021 08:19:21: 14000000 INFO @ Sat, 03 Apr 2021 08:19:22: 4000000 INFO @ Sat, 03 Apr 2021 08:19:24: 9000000 INFO @ Sat, 03 Apr 2021 08:19:28: 15000000 INFO @ Sat, 03 Apr 2021 08:19:29: 5000000 INFO @ Sat, 03 Apr 2021 08:19:31: 10000000 INFO @ Sat, 03 Apr 2021 08:19:35: 16000000 INFO @ Sat, 03 Apr 2021 08:19:36: 6000000 INFO @ Sat, 03 Apr 2021 08:19:38: 11000000 INFO @ Sat, 03 Apr 2021 08:19:41: 17000000 INFO @ Sat, 03 Apr 2021 08:19:44: 7000000 INFO @ Sat, 03 Apr 2021 08:19:45: 12000000 INFO @ Sat, 03 Apr 2021 08:19:47: 18000000 INFO @ Sat, 03 Apr 2021 08:19:51: 8000000 INFO @ Sat, 03 Apr 2021 08:19:52: 13000000 INFO @ Sat, 03 Apr 2021 08:19:54: 19000000 INFO @ Sat, 03 Apr 2021 08:19:58: 9000000 INFO @ Sat, 03 Apr 2021 08:19:59: 14000000 INFO @ Sat, 03 Apr 2021 08:20:00: 20000000 INFO @ Sat, 03 Apr 2021 08:20:05: 10000000 INFO @ Sat, 03 Apr 2021 08:20:06: 21000000 INFO @ Sat, 03 Apr 2021 08:20:06: 15000000 INFO @ Sat, 03 Apr 2021 08:20:12: 11000000 INFO @ Sat, 03 Apr 2021 08:20:12: 22000000 INFO @ Sat, 03 Apr 2021 08:20:13: 16000000 INFO @ Sat, 03 Apr 2021 08:20:19: 23000000 INFO @ Sat, 03 Apr 2021 08:20:19: 12000000 INFO @ Sat, 03 Apr 2021 08:20:20: 17000000 INFO @ Sat, 03 Apr 2021 08:20:25: 24000000 INFO @ Sat, 03 Apr 2021 08:20:26: 13000000 INFO @ Sat, 03 Apr 2021 08:20:27: 18000000 INFO @ Sat, 03 Apr 2021 08:20:31: 25000000 INFO @ Sat, 03 Apr 2021 08:20:34: 14000000 INFO @ Sat, 03 Apr 2021 08:20:35: 19000000 INFO @ Sat, 03 Apr 2021 08:20:38: 26000000 INFO @ Sat, 03 Apr 2021 08:20:41: 15000000 INFO @ Sat, 03 Apr 2021 08:20:42: 20000000 INFO @ Sat, 03 Apr 2021 08:20:44: 27000000 INFO @ Sat, 03 Apr 2021 08:20:48: 16000000 INFO @ Sat, 03 Apr 2021 08:20:49: 21000000 INFO @ Sat, 03 Apr 2021 08:20:51: 28000000 INFO @ Sat, 03 Apr 2021 08:20:56: 17000000 INFO @ Sat, 03 Apr 2021 08:20:57: 22000000 INFO @ Sat, 03 Apr 2021 08:20:58: 29000000 INFO @ Sat, 03 Apr 2021 08:21:03: 18000000 INFO @ Sat, 03 Apr 2021 08:21:04: 23000000 INFO @ Sat, 03 Apr 2021 08:21:04: 30000000 INFO @ Sat, 03 Apr 2021 08:21:10: 19000000 INFO @ Sat, 03 Apr 2021 08:21:11: 24000000 INFO @ Sat, 03 Apr 2021 08:21:12: 31000000 INFO @ Sat, 03 Apr 2021 08:21:17: 20000000 INFO @ Sat, 03 Apr 2021 08:21:18: 32000000 INFO @ Sat, 03 Apr 2021 08:21:18: 25000000 INFO @ Sat, 03 Apr 2021 08:21:24: 33000000 INFO @ Sat, 03 Apr 2021 08:21:25: 21000000 INFO @ Sat, 03 Apr 2021 08:21:25: 26000000 INFO @ Sat, 03 Apr 2021 08:21:30: 34000000 INFO @ Sat, 03 Apr 2021 08:21:32: 27000000 INFO @ Sat, 03 Apr 2021 08:21:32: 22000000 INFO @ Sat, 03 Apr 2021 08:21:36: 35000000 INFO @ Sat, 03 Apr 2021 08:21:39: 28000000 INFO @ Sat, 03 Apr 2021 08:21:39: 23000000 INFO @ Sat, 03 Apr 2021 08:21:42: 36000000 INFO @ Sat, 03 Apr 2021 08:21:46: 29000000 INFO @ Sat, 03 Apr 2021 08:21:46: 24000000 INFO @ Sat, 03 Apr 2021 08:21:48: 37000000 INFO @ Sat, 03 Apr 2021 08:21:53: 30000000 INFO @ Sat, 03 Apr 2021 08:21:53: 25000000 INFO @ Sat, 03 Apr 2021 08:21:55: 38000000 INFO @ Sat, 03 Apr 2021 08:22:00: 31000000 INFO @ Sat, 03 Apr 2021 08:22:01: 26000000 INFO @ Sat, 03 Apr 2021 08:22:01: 39000000 INFO @ Sat, 03 Apr 2021 08:22:07: 32000000 INFO @ Sat, 03 Apr 2021 08:22:08: 40000000 INFO @ Sat, 03 Apr 2021 08:22:08: 27000000 INFO @ Sat, 03 Apr 2021 08:22:14: 41000000 INFO @ Sat, 03 Apr 2021 08:22:15: 33000000 INFO @ Sat, 03 Apr 2021 08:22:15: 28000000 INFO @ Sat, 03 Apr 2021 08:22:21: 42000000 INFO @ Sat, 03 Apr 2021 08:22:22: 34000000 INFO @ Sat, 03 Apr 2021 08:22:22: 29000000 INFO @ Sat, 03 Apr 2021 08:22:27: 43000000 INFO @ Sat, 03 Apr 2021 08:22:29: 35000000 INFO @ Sat, 03 Apr 2021 08:22:29: 30000000 INFO @ Sat, 03 Apr 2021 08:22:34: 44000000 INFO @ Sat, 03 Apr 2021 08:22:36: 36000000 INFO @ Sat, 03 Apr 2021 08:22:37: 31000000 INFO @ Sat, 03 Apr 2021 08:22:41: 45000000 INFO @ Sat, 03 Apr 2021 08:22:43: 37000000 INFO @ Sat, 03 Apr 2021 08:22:44: 32000000 INFO @ Sat, 03 Apr 2021 08:22:48: 46000000 INFO @ Sat, 03 Apr 2021 08:22:50: 38000000 INFO @ Sat, 03 Apr 2021 08:22:51: 33000000 INFO @ Sat, 03 Apr 2021 08:22:54: 47000000 INFO @ Sat, 03 Apr 2021 08:22:57: 39000000 INFO @ Sat, 03 Apr 2021 08:22:58: 34000000 INFO @ Sat, 03 Apr 2021 08:23:00: 48000000 INFO @ Sat, 03 Apr 2021 08:23:04: 40000000 INFO @ Sat, 03 Apr 2021 08:23:05: 35000000 INFO @ Sat, 03 Apr 2021 08:23:07: 49000000 INFO @ Sat, 03 Apr 2021 08:23:11: 41000000 INFO @ Sat, 03 Apr 2021 08:23:12: 36000000 INFO @ Sat, 03 Apr 2021 08:23:13: 50000000 INFO @ Sat, 03 Apr 2021 08:23:18: 42000000 INFO @ Sat, 03 Apr 2021 08:23:19: 51000000 INFO @ Sat, 03 Apr 2021 08:23:19: 37000000 INFO @ Sat, 03 Apr 2021 08:23:25: 43000000 INFO @ Sat, 03 Apr 2021 08:23:25: 52000000 INFO @ Sat, 03 Apr 2021 08:23:27: 38000000 INFO @ Sat, 03 Apr 2021 08:23:31: 53000000 INFO @ Sat, 03 Apr 2021 08:23:32: 44000000 INFO @ Sat, 03 Apr 2021 08:23:34: 39000000 INFO @ Sat, 03 Apr 2021 08:23:38: 54000000 INFO @ Sat, 03 Apr 2021 08:23:39: 45000000 INFO @ Sat, 03 Apr 2021 08:23:41: 40000000 INFO @ Sat, 03 Apr 2021 08:23:44: 55000000 INFO @ Sat, 03 Apr 2021 08:23:46: 46000000 INFO @ Sat, 03 Apr 2021 08:23:48: 41000000 INFO @ Sat, 03 Apr 2021 08:23:50: 56000000 INFO @ Sat, 03 Apr 2021 08:23:53: 47000000 INFO @ Sat, 03 Apr 2021 08:23:55: 42000000 INFO @ Sat, 03 Apr 2021 08:23:57: 57000000 INFO @ Sat, 03 Apr 2021 08:24:00: 48000000 INFO @ Sat, 03 Apr 2021 08:24:02: 43000000 INFO @ Sat, 03 Apr 2021 08:24:04: 58000000 INFO @ Sat, 03 Apr 2021 08:24:07: 49000000 INFO @ Sat, 03 Apr 2021 08:24:10: 44000000 INFO @ Sat, 03 Apr 2021 08:24:11: 59000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:24:15: 50000000 INFO @ Sat, 03 Apr 2021 08:24:17: 45000000 INFO @ Sat, 03 Apr 2021 08:24:17: 60000000 INFO @ Sat, 03 Apr 2021 08:24:22: 51000000 INFO @ Sat, 03 Apr 2021 08:24:24: 46000000 INFO @ Sat, 03 Apr 2021 08:24:24: 61000000 INFO @ Sat, 03 Apr 2021 08:24:29: 52000000 INFO @ Sat, 03 Apr 2021 08:24:30: 62000000 INFO @ Sat, 03 Apr 2021 08:24:31: 47000000 INFO @ Sat, 03 Apr 2021 08:24:35: 53000000 INFO @ Sat, 03 Apr 2021 08:24:36: 63000000 INFO @ Sat, 03 Apr 2021 08:24:38: 48000000 INFO @ Sat, 03 Apr 2021 08:24:42: 64000000 INFO @ Sat, 03 Apr 2021 08:24:42: 54000000 INFO @ Sat, 03 Apr 2021 08:24:45: 49000000 INFO @ Sat, 03 Apr 2021 08:24:48: 65000000 INFO @ Sat, 03 Apr 2021 08:24:49: 55000000 INFO @ Sat, 03 Apr 2021 08:24:52: 50000000 INFO @ Sat, 03 Apr 2021 08:24:55: 66000000 INFO @ Sat, 03 Apr 2021 08:24:57: 56000000 INFO @ Sat, 03 Apr 2021 08:24:59: 51000000 INFO @ Sat, 03 Apr 2021 08:25:01: 67000000 INFO @ Sat, 03 Apr 2021 08:25:04: 57000000 INFO @ Sat, 03 Apr 2021 08:25:06: 52000000 INFO @ Sat, 03 Apr 2021 08:25:07: 68000000 INFO @ Sat, 03 Apr 2021 08:25:11: 58000000 INFO @ Sat, 03 Apr 2021 08:25:11: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:25:11: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:25:11: #1 total tags in treatment: 27126351 INFO @ Sat, 03 Apr 2021 08:25:11: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:25:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:25:12: #1 tags after filtering in treatment: 18173569 INFO @ Sat, 03 Apr 2021 08:25:12: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 08:25:12: #1 finished! INFO @ Sat, 03 Apr 2021 08:25:12: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:25:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:25:12: 53000000 INFO @ Sat, 03 Apr 2021 08:25:13: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 08:25:13: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 08:25:13: start model_add_line... INFO @ Sat, 03 Apr 2021 08:25:13: start X-correlation... INFO @ Sat, 03 Apr 2021 08:25:13: end of X-cor INFO @ Sat, 03 Apr 2021 08:25:13: #2 finished! INFO @ Sat, 03 Apr 2021 08:25:13: #2 predicted fragment length is 83 bps INFO @ Sat, 03 Apr 2021 08:25:13: #2 alternative fragment length(s) may be 4,83,597 bps INFO @ Sat, 03 Apr 2021 08:25:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.05_model.r WARNING @ Sat, 03 Apr 2021 08:25:13: #2 Since the d (83) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:25:13: #2 You may need to consider one of the other alternative d(s): 4,83,597 WARNING @ Sat, 03 Apr 2021 08:25:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:25:13: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:25:13: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:25:18: 59000000 INFO @ Sat, 03 Apr 2021 08:25:19: 54000000 INFO @ Sat, 03 Apr 2021 08:25:25: 60000000 INFO @ Sat, 03 Apr 2021 08:25:27: 55000000 INFO @ Sat, 03 Apr 2021 08:25:32: 61000000 INFO @ Sat, 03 Apr 2021 08:25:34: 56000000 INFO @ Sat, 03 Apr 2021 08:25:38: 62000000 INFO @ Sat, 03 Apr 2021 08:25:41: 57000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:25:45: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:25:45: 63000000 INFO @ Sat, 03 Apr 2021 08:25:48: 58000000 INFO @ Sat, 03 Apr 2021 08:25:52: 64000000 INFO @ Sat, 03 Apr 2021 08:25:55: 59000000 INFO @ Sat, 03 Apr 2021 08:25:59: 65000000 INFO @ Sat, 03 Apr 2021 08:26:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.05_peaks.xls INFO @ Sat, 03 Apr 2021 08:26:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:26:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.05_summits.bed INFO @ Sat, 03 Apr 2021 08:26:02: Done! INFO @ Sat, 03 Apr 2021 08:26:02: 60000000 pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (10186 records, 4 fields): 20 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:26:06: 66000000 INFO @ Sat, 03 Apr 2021 08:26:08: 61000000 INFO @ Sat, 03 Apr 2021 08:26:13: 67000000 INFO @ Sat, 03 Apr 2021 08:26:15: 62000000 INFO @ Sat, 03 Apr 2021 08:26:20: 68000000 INFO @ Sat, 03 Apr 2021 08:26:22: 63000000 INFO @ Sat, 03 Apr 2021 08:26:25: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:26:25: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:26:25: #1 total tags in treatment: 27126351 INFO @ Sat, 03 Apr 2021 08:26:25: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:26:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:26:26: #1 tags after filtering in treatment: 18173569 INFO @ Sat, 03 Apr 2021 08:26:26: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 08:26:26: #1 finished! INFO @ Sat, 03 Apr 2021 08:26:26: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:26:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:26:27: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 08:26:27: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 08:26:27: start model_add_line... INFO @ Sat, 03 Apr 2021 08:26:27: start X-correlation... INFO @ Sat, 03 Apr 2021 08:26:27: end of X-cor INFO @ Sat, 03 Apr 2021 08:26:27: #2 finished! INFO @ Sat, 03 Apr 2021 08:26:27: #2 predicted fragment length is 83 bps INFO @ Sat, 03 Apr 2021 08:26:27: #2 alternative fragment length(s) may be 4,83,597 bps INFO @ Sat, 03 Apr 2021 08:26:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.10_model.r WARNING @ Sat, 03 Apr 2021 08:26:27: #2 Since the d (83) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:26:27: #2 You may need to consider one of the other alternative d(s): 4,83,597 WARNING @ Sat, 03 Apr 2021 08:26:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:26:27: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:26:27: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:26:29: 64000000 INFO @ Sat, 03 Apr 2021 08:26:35: 65000000 INFO @ Sat, 03 Apr 2021 08:26:41: 66000000 INFO @ Sat, 03 Apr 2021 08:26:47: 67000000 INFO @ Sat, 03 Apr 2021 08:26:53: 68000000 INFO @ Sat, 03 Apr 2021 08:26:58: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 08:26:58: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 08:26:58: #1 total tags in treatment: 27126351 INFO @ Sat, 03 Apr 2021 08:26:58: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:26:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:26:59: #1 tags after filtering in treatment: 18173569 INFO @ Sat, 03 Apr 2021 08:26:59: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 03 Apr 2021 08:26:59: #1 finished! INFO @ Sat, 03 Apr 2021 08:26:59: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:26:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:26:59: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:27:00: #2 number of paired peaks: 159 WARNING @ Sat, 03 Apr 2021 08:27:00: Fewer paired peaks (159) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 159 pairs to build model! INFO @ Sat, 03 Apr 2021 08:27:00: start model_add_line... INFO @ Sat, 03 Apr 2021 08:27:00: start X-correlation... INFO @ Sat, 03 Apr 2021 08:27:00: end of X-cor INFO @ Sat, 03 Apr 2021 08:27:00: #2 finished! INFO @ Sat, 03 Apr 2021 08:27:00: #2 predicted fragment length is 83 bps INFO @ Sat, 03 Apr 2021 08:27:00: #2 alternative fragment length(s) may be 4,83,597 bps INFO @ Sat, 03 Apr 2021 08:27:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.20_model.r WARNING @ Sat, 03 Apr 2021 08:27:00: #2 Since the d (83) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 08:27:00: #2 You may need to consider one of the other alternative d(s): 4,83,597 WARNING @ Sat, 03 Apr 2021 08:27:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 08:27:00: #3 Call peaks... INFO @ Sat, 03 Apr 2021 08:27:00: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 08:27:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:27:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:27:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.10_summits.bed INFO @ Sat, 03 Apr 2021 08:27:15: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (5635 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:27:31: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:27:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:27:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:27:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029124/SRX3029124.20_summits.bed INFO @ Sat, 03 Apr 2021 08:27:47: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (2082 records, 4 fields): 4 millis CompletedMACS2peakCalling