Job ID = 6626335 SRX = SRX3008763 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39763228 spots for SRR5831445/SRR5831445.sra Written 39763228 spots for SRR5831445/SRR5831445.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626582 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:16 39763228 reads; of these: 39763228 (100.00%) were unpaired; of these: 1220721 (3.07%) aligned 0 times 32310876 (81.26%) aligned exactly 1 time 6231631 (15.67%) aligned >1 times 96.93% overall alignment rate Time searching: 00:08:16 Overall time: 00:08:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6494594 / 38542507 = 0.1685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:12:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:12:35: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:12:35: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:12:41: 1000000 INFO @ Tue, 14 Jul 2020 07:12:46: 2000000 INFO @ Tue, 14 Jul 2020 07:12:52: 3000000 INFO @ Tue, 14 Jul 2020 07:12:57: 4000000 INFO @ Tue, 14 Jul 2020 07:13:03: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:13:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:13:05: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:13:05: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:13:09: 6000000 INFO @ Tue, 14 Jul 2020 07:13:11: 1000000 INFO @ Tue, 14 Jul 2020 07:13:15: 7000000 INFO @ Tue, 14 Jul 2020 07:13:17: 2000000 INFO @ Tue, 14 Jul 2020 07:13:20: 8000000 INFO @ Tue, 14 Jul 2020 07:13:23: 3000000 INFO @ Tue, 14 Jul 2020 07:13:26: 9000000 INFO @ Tue, 14 Jul 2020 07:13:29: 4000000 INFO @ Tue, 14 Jul 2020 07:13:32: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:13:35: 5000000 INFO @ Tue, 14 Jul 2020 07:13:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:13:35: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:13:35: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:13:38: 11000000 INFO @ Tue, 14 Jul 2020 07:13:41: 6000000 INFO @ Tue, 14 Jul 2020 07:13:41: 1000000 INFO @ Tue, 14 Jul 2020 07:13:44: 12000000 INFO @ Tue, 14 Jul 2020 07:13:47: 7000000 INFO @ Tue, 14 Jul 2020 07:13:47: 2000000 INFO @ Tue, 14 Jul 2020 07:13:50: 13000000 INFO @ Tue, 14 Jul 2020 07:13:52: 3000000 INFO @ Tue, 14 Jul 2020 07:13:52: 8000000 INFO @ Tue, 14 Jul 2020 07:13:55: 14000000 INFO @ Tue, 14 Jul 2020 07:13:58: 4000000 INFO @ Tue, 14 Jul 2020 07:13:58: 9000000 INFO @ Tue, 14 Jul 2020 07:14:01: 15000000 INFO @ Tue, 14 Jul 2020 07:14:03: 5000000 INFO @ Tue, 14 Jul 2020 07:14:04: 10000000 INFO @ Tue, 14 Jul 2020 07:14:07: 16000000 INFO @ Tue, 14 Jul 2020 07:14:08: 6000000 INFO @ Tue, 14 Jul 2020 07:14:10: 11000000 INFO @ Tue, 14 Jul 2020 07:14:13: 17000000 INFO @ Tue, 14 Jul 2020 07:14:14: 7000000 INFO @ Tue, 14 Jul 2020 07:14:16: 12000000 INFO @ Tue, 14 Jul 2020 07:14:19: 8000000 INFO @ Tue, 14 Jul 2020 07:14:19: 18000000 INFO @ Tue, 14 Jul 2020 07:14:22: 13000000 INFO @ Tue, 14 Jul 2020 07:14:25: 9000000 INFO @ Tue, 14 Jul 2020 07:14:25: 19000000 INFO @ Tue, 14 Jul 2020 07:14:28: 14000000 INFO @ Tue, 14 Jul 2020 07:14:30: 10000000 INFO @ Tue, 14 Jul 2020 07:14:31: 20000000 INFO @ Tue, 14 Jul 2020 07:14:34: 15000000 INFO @ Tue, 14 Jul 2020 07:14:36: 11000000 INFO @ Tue, 14 Jul 2020 07:14:37: 21000000 INFO @ Tue, 14 Jul 2020 07:14:40: 16000000 INFO @ Tue, 14 Jul 2020 07:14:41: 12000000 INFO @ Tue, 14 Jul 2020 07:14:43: 22000000 INFO @ Tue, 14 Jul 2020 07:14:47: 13000000 INFO @ Tue, 14 Jul 2020 07:14:47: 17000000 INFO @ Tue, 14 Jul 2020 07:14:49: 23000000 INFO @ Tue, 14 Jul 2020 07:14:52: 14000000 INFO @ Tue, 14 Jul 2020 07:14:53: 18000000 INFO @ Tue, 14 Jul 2020 07:14:55: 24000000 INFO @ Tue, 14 Jul 2020 07:14:58: 15000000 INFO @ Tue, 14 Jul 2020 07:14:59: 19000000 INFO @ Tue, 14 Jul 2020 07:15:01: 25000000 INFO @ Tue, 14 Jul 2020 07:15:04: 16000000 INFO @ Tue, 14 Jul 2020 07:15:05: 20000000 INFO @ Tue, 14 Jul 2020 07:15:07: 26000000 INFO @ Tue, 14 Jul 2020 07:15:10: 17000000 INFO @ Tue, 14 Jul 2020 07:15:11: 21000000 INFO @ Tue, 14 Jul 2020 07:15:13: 27000000 INFO @ Tue, 14 Jul 2020 07:15:16: 18000000 INFO @ Tue, 14 Jul 2020 07:15:17: 22000000 INFO @ Tue, 14 Jul 2020 07:15:19: 28000000 INFO @ Tue, 14 Jul 2020 07:15:21: 19000000 INFO @ Tue, 14 Jul 2020 07:15:23: 23000000 INFO @ Tue, 14 Jul 2020 07:15:25: 29000000 INFO @ Tue, 14 Jul 2020 07:15:27: 20000000 INFO @ Tue, 14 Jul 2020 07:15:29: 24000000 INFO @ Tue, 14 Jul 2020 07:15:31: 30000000 INFO @ Tue, 14 Jul 2020 07:15:33: 21000000 INFO @ Tue, 14 Jul 2020 07:15:36: 25000000 INFO @ Tue, 14 Jul 2020 07:15:37: 31000000 INFO @ Tue, 14 Jul 2020 07:15:39: 22000000 INFO @ Tue, 14 Jul 2020 07:15:42: 26000000 INFO @ Tue, 14 Jul 2020 07:15:43: 32000000 INFO @ Tue, 14 Jul 2020 07:15:43: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:15:43: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:15:43: #1 total tags in treatment: 32047913 INFO @ Tue, 14 Jul 2020 07:15:43: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:15:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:15:44: #1 tags after filtering in treatment: 32047913 INFO @ Tue, 14 Jul 2020 07:15:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:15:44: #1 finished! INFO @ Tue, 14 Jul 2020 07:15:44: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:15:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:15:45: 23000000 INFO @ Tue, 14 Jul 2020 07:15:46: #2 number of paired peaks: 72 WARNING @ Tue, 14 Jul 2020 07:15:46: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:15:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:15:48: 27000000 INFO @ Tue, 14 Jul 2020 07:15:51: 24000000 INFO @ Tue, 14 Jul 2020 07:15:54: 28000000 INFO @ Tue, 14 Jul 2020 07:15:56: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:16:00: 29000000 INFO @ Tue, 14 Jul 2020 07:16:02: 26000000 INFO @ Tue, 14 Jul 2020 07:16:06: 30000000 INFO @ Tue, 14 Jul 2020 07:16:08: 27000000 INFO @ Tue, 14 Jul 2020 07:16:12: 31000000 INFO @ Tue, 14 Jul 2020 07:16:13: 28000000 INFO @ Tue, 14 Jul 2020 07:16:18: 32000000 INFO @ Tue, 14 Jul 2020 07:16:19: 29000000 INFO @ Tue, 14 Jul 2020 07:16:19: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:16:19: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:16:19: #1 total tags in treatment: 32047913 INFO @ Tue, 14 Jul 2020 07:16:19: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:16:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:16:19: #1 tags after filtering in treatment: 32047913 INFO @ Tue, 14 Jul 2020 07:16:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:16:19: #1 finished! INFO @ Tue, 14 Jul 2020 07:16:19: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:16:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:16:21: #2 number of paired peaks: 72 WARNING @ Tue, 14 Jul 2020 07:16:21: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:16:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:16:24: 30000000 INFO @ Tue, 14 Jul 2020 07:16:30: 31000000 INFO @ Tue, 14 Jul 2020 07:16:35: 32000000 INFO @ Tue, 14 Jul 2020 07:16:36: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:16:36: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:16:36: #1 total tags in treatment: 32047913 INFO @ Tue, 14 Jul 2020 07:16:36: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:16:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:16:36: #1 tags after filtering in treatment: 32047913 INFO @ Tue, 14 Jul 2020 07:16:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:16:36: #1 finished! INFO @ Tue, 14 Jul 2020 07:16:36: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:16:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:16:38: #2 number of paired peaks: 72 WARNING @ Tue, 14 Jul 2020 07:16:38: Too few paired peaks (72) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:16:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008763/SRX3008763.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。