Job ID = 6626333 SRX = SRX3008761 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 46495373 spots for SRR5831443/SRR5831443.sra Written 46495373 spots for SRR5831443/SRR5831443.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626597 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:10:38 46495373 reads; of these: 46495373 (100.00%) were unpaired; of these: 7329253 (15.76%) aligned 0 times 32700116 (70.33%) aligned exactly 1 time 6466004 (13.91%) aligned >1 times 84.24% overall alignment rate Time searching: 00:10:39 Overall time: 00:10:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6571981 / 39166120 = 0.1678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:16:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:16:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:16:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:16:39: 1000000 INFO @ Tue, 14 Jul 2020 07:16:44: 2000000 INFO @ Tue, 14 Jul 2020 07:16:50: 3000000 INFO @ Tue, 14 Jul 2020 07:16:55: 4000000 INFO @ Tue, 14 Jul 2020 07:17:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:17:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:17:03: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:17:03: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:17:07: 6000000 INFO @ Tue, 14 Jul 2020 07:17:11: 1000000 INFO @ Tue, 14 Jul 2020 07:17:14: 7000000 INFO @ Tue, 14 Jul 2020 07:17:18: 2000000 INFO @ Tue, 14 Jul 2020 07:17:20: 8000000 INFO @ Tue, 14 Jul 2020 07:17:26: 3000000 INFO @ Tue, 14 Jul 2020 07:17:27: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:17:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:17:33: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:17:33: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:17:34: 10000000 INFO @ Tue, 14 Jul 2020 07:17:34: 4000000 INFO @ Tue, 14 Jul 2020 07:17:40: 11000000 INFO @ Tue, 14 Jul 2020 07:17:42: 5000000 INFO @ Tue, 14 Jul 2020 07:17:43: 1000000 INFO @ Tue, 14 Jul 2020 07:17:47: 12000000 INFO @ Tue, 14 Jul 2020 07:17:49: 6000000 INFO @ Tue, 14 Jul 2020 07:17:52: 2000000 INFO @ Tue, 14 Jul 2020 07:17:54: 13000000 INFO @ Tue, 14 Jul 2020 07:17:57: 7000000 INFO @ Tue, 14 Jul 2020 07:18:01: 14000000 INFO @ Tue, 14 Jul 2020 07:18:02: 3000000 INFO @ Tue, 14 Jul 2020 07:18:05: 8000000 INFO @ Tue, 14 Jul 2020 07:18:08: 15000000 INFO @ Tue, 14 Jul 2020 07:18:11: 4000000 INFO @ Tue, 14 Jul 2020 07:18:12: 9000000 INFO @ Tue, 14 Jul 2020 07:18:14: 16000000 INFO @ Tue, 14 Jul 2020 07:18:20: 10000000 INFO @ Tue, 14 Jul 2020 07:18:20: 5000000 INFO @ Tue, 14 Jul 2020 07:18:21: 17000000 INFO @ Tue, 14 Jul 2020 07:18:28: 18000000 INFO @ Tue, 14 Jul 2020 07:18:28: 11000000 INFO @ Tue, 14 Jul 2020 07:18:29: 6000000 INFO @ Tue, 14 Jul 2020 07:18:34: 19000000 INFO @ Tue, 14 Jul 2020 07:18:35: 12000000 INFO @ Tue, 14 Jul 2020 07:18:38: 7000000 INFO @ Tue, 14 Jul 2020 07:18:41: 20000000 INFO @ Tue, 14 Jul 2020 07:18:43: 13000000 INFO @ Tue, 14 Jul 2020 07:18:47: 8000000 INFO @ Tue, 14 Jul 2020 07:18:48: 21000000 INFO @ Tue, 14 Jul 2020 07:18:51: 14000000 INFO @ Tue, 14 Jul 2020 07:18:54: 22000000 INFO @ Tue, 14 Jul 2020 07:18:56: 9000000 INFO @ Tue, 14 Jul 2020 07:18:58: 15000000 INFO @ Tue, 14 Jul 2020 07:19:01: 23000000 INFO @ Tue, 14 Jul 2020 07:19:04: 10000000 INFO @ Tue, 14 Jul 2020 07:19:06: 16000000 INFO @ Tue, 14 Jul 2020 07:19:08: 24000000 INFO @ Tue, 14 Jul 2020 07:19:13: 11000000 INFO @ Tue, 14 Jul 2020 07:19:14: 17000000 INFO @ Tue, 14 Jul 2020 07:19:14: 25000000 INFO @ Tue, 14 Jul 2020 07:19:21: 18000000 INFO @ Tue, 14 Jul 2020 07:19:21: 26000000 INFO @ Tue, 14 Jul 2020 07:19:22: 12000000 INFO @ Tue, 14 Jul 2020 07:19:28: 27000000 INFO @ Tue, 14 Jul 2020 07:19:29: 19000000 INFO @ Tue, 14 Jul 2020 07:19:31: 13000000 INFO @ Tue, 14 Jul 2020 07:19:35: 28000000 INFO @ Tue, 14 Jul 2020 07:19:36: 20000000 INFO @ Tue, 14 Jul 2020 07:19:40: 14000000 INFO @ Tue, 14 Jul 2020 07:19:42: 29000000 INFO @ Tue, 14 Jul 2020 07:19:44: 21000000 INFO @ Tue, 14 Jul 2020 07:19:48: 30000000 INFO @ Tue, 14 Jul 2020 07:19:49: 15000000 INFO @ Tue, 14 Jul 2020 07:19:52: 22000000 INFO @ Tue, 14 Jul 2020 07:19:56: 31000000 INFO @ Tue, 14 Jul 2020 07:19:57: 16000000 INFO @ Tue, 14 Jul 2020 07:19:59: 23000000 INFO @ Tue, 14 Jul 2020 07:20:02: 32000000 INFO @ Tue, 14 Jul 2020 07:20:05: 17000000 INFO @ Tue, 14 Jul 2020 07:20:07: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:20:07: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:20:07: #1 total tags in treatment: 32594139 INFO @ Tue, 14 Jul 2020 07:20:07: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:20:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:20:07: 24000000 INFO @ Tue, 14 Jul 2020 07:20:07: #1 tags after filtering in treatment: 32594139 INFO @ Tue, 14 Jul 2020 07:20:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:20:07: #1 finished! INFO @ Tue, 14 Jul 2020 07:20:07: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:20:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:20:09: #2 number of paired peaks: 62 WARNING @ Tue, 14 Jul 2020 07:20:09: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:20:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:20:13: 18000000 INFO @ Tue, 14 Jul 2020 07:20:14: 25000000 INFO @ Tue, 14 Jul 2020 07:20:20: 19000000 INFO @ Tue, 14 Jul 2020 07:20:22: 26000000 INFO @ Tue, 14 Jul 2020 07:20:28: 20000000 INFO @ Tue, 14 Jul 2020 07:20:30: 27000000 INFO @ Tue, 14 Jul 2020 07:20:35: 21000000 INFO @ Tue, 14 Jul 2020 07:20:37: 28000000 INFO @ Tue, 14 Jul 2020 07:20:43: 22000000 INFO @ Tue, 14 Jul 2020 07:20:45: 29000000 INFO @ Tue, 14 Jul 2020 07:20:50: 23000000 INFO @ Tue, 14 Jul 2020 07:20:53: 30000000 INFO @ Tue, 14 Jul 2020 07:20:57: 24000000 INFO @ Tue, 14 Jul 2020 07:21:01: 31000000 INFO @ Tue, 14 Jul 2020 07:21:05: 25000000 INFO @ Tue, 14 Jul 2020 07:21:09: 32000000 INFO @ Tue, 14 Jul 2020 07:21:12: 26000000 INFO @ Tue, 14 Jul 2020 07:21:13: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:21:13: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:21:13: #1 total tags in treatment: 32594139 INFO @ Tue, 14 Jul 2020 07:21:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:21:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:21:14: #1 tags after filtering in treatment: 32594139 INFO @ Tue, 14 Jul 2020 07:21:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:21:14: #1 finished! INFO @ Tue, 14 Jul 2020 07:21:14: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:21:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:21:16: #2 number of paired peaks: 62 WARNING @ Tue, 14 Jul 2020 07:21:16: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:21:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:21:19: 27000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:21:26: 28000000 INFO @ Tue, 14 Jul 2020 07:21:33: 29000000 INFO @ Tue, 14 Jul 2020 07:21:41: 30000000 INFO @ Tue, 14 Jul 2020 07:21:48: 31000000 INFO @ Tue, 14 Jul 2020 07:21:55: 32000000 INFO @ Tue, 14 Jul 2020 07:21:59: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:21:59: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:21:59: #1 total tags in treatment: 32594139 INFO @ Tue, 14 Jul 2020 07:21:59: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:21:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:22:00: #1 tags after filtering in treatment: 32594139 INFO @ Tue, 14 Jul 2020 07:22:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:22:00: #1 finished! INFO @ Tue, 14 Jul 2020 07:22:00: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:22:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:22:02: #2 number of paired peaks: 62 WARNING @ Tue, 14 Jul 2020 07:22:02: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:22:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008761/SRX3008761.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling