Job ID = 10714372 sra ファイルのダウンロード中... Completed: 5760689K bytes transferred in 565 seconds (83497K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 39983162 spots for /home/okishinya/chipatlas/results/ce10/SRX2985937/SRR5807087.sra Written 39983162 spots for /home/okishinya/chipatlas/results/ce10/SRX2985937/SRR5807087.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:18:24 39983162 reads; of these: 39983162 (100.00%) were paired; of these: 848041 (2.12%) aligned concordantly 0 times 33613182 (84.07%) aligned concordantly exactly 1 time 5521939 (13.81%) aligned concordantly >1 times ---- 848041 pairs aligned concordantly 0 times; of these: 64167 (7.57%) aligned discordantly 1 time ---- 783874 pairs aligned 0 times concordantly or discordantly; of these: 1567748 mates make up the pairs; of these: 967533 (61.71%) aligned 0 times 439083 (28.01%) aligned exactly 1 time 161132 (10.28%) aligned >1 times 98.79% overall alignment rate Time searching: 01:18:24 Overall time: 01:18:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 6240088 / 39153567 = 0.1594 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 03 Jun 2018 14:36:41: # Command line: callpeak -t SRX2985937.bam -f BAM -g ce -n SRX2985937.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2985937.20 # format = BAM # ChIP-seq file = ['SRX2985937.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 14:36:41: #1 read tag files... INFO @ Sun, 03 Jun 2018 14:36:41: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 14:36:41: # Command line: callpeak -t SRX2985937.bam -f BAM -g ce -n SRX2985937.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2985937.10 # format = BAM # ChIP-seq file = ['SRX2985937.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 14:36:41: #1 read tag files... INFO @ Sun, 03 Jun 2018 14:36:41: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 14:36:41: # Command line: callpeak -t SRX2985937.bam -f BAM -g ce -n SRX2985937.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2985937.05 # format = BAM # ChIP-seq file = ['SRX2985937.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 14:36:41: #1 read tag files... INFO @ Sun, 03 Jun 2018 14:36:41: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 14:36:48: 1000000 INFO @ Sun, 03 Jun 2018 14:36:48: 1000000 INFO @ Sun, 03 Jun 2018 14:36:49: 1000000 INFO @ Sun, 03 Jun 2018 14:36:56: 2000000 INFO @ Sun, 03 Jun 2018 14:36:56: 2000000 INFO @ Sun, 03 Jun 2018 14:36:56: 2000000 INFO @ Sun, 03 Jun 2018 14:37:03: 3000000 INFO @ Sun, 03 Jun 2018 14:37:03: 3000000 INFO @ Sun, 03 Jun 2018 14:37:03: 3000000 INFO @ Sun, 03 Jun 2018 14:37:10: 4000000 INFO @ Sun, 03 Jun 2018 14:37:11: 4000000 INFO @ Sun, 03 Jun 2018 14:37:11: 4000000 INFO @ Sun, 03 Jun 2018 14:37:17: 5000000 INFO @ Sun, 03 Jun 2018 14:37:18: 5000000 INFO @ Sun, 03 Jun 2018 14:37:19: 5000000 INFO @ Sun, 03 Jun 2018 14:37:25: 6000000 INFO @ Sun, 03 Jun 2018 14:37:26: 6000000 INFO @ Sun, 03 Jun 2018 14:37:27: 6000000 INFO @ Sun, 03 Jun 2018 14:37:32: 7000000 INFO @ Sun, 03 Jun 2018 14:37:34: 7000000 INFO @ Sun, 03 Jun 2018 14:37:35: 7000000 INFO @ Sun, 03 Jun 2018 14:37:39: 8000000 INFO @ Sun, 03 Jun 2018 14:37:42: 8000000 INFO @ Sun, 03 Jun 2018 14:37:43: 8000000 INFO @ Sun, 03 Jun 2018 14:37:46: 9000000 INFO @ Sun, 03 Jun 2018 14:37:49: 9000000 INFO @ Sun, 03 Jun 2018 14:37:50: 9000000 INFO @ Sun, 03 Jun 2018 14:37:54: 10000000 INFO @ Sun, 03 Jun 2018 14:37:57: 10000000 INFO @ Sun, 03 Jun 2018 14:37:58: 10000000 INFO @ Sun, 03 Jun 2018 14:38:01: 11000000 INFO @ Sun, 03 Jun 2018 14:38:05: 11000000 INFO @ Sun, 03 Jun 2018 14:38:06: 11000000 INFO @ Sun, 03 Jun 2018 14:38:08: 12000000 INFO @ Sun, 03 Jun 2018 14:38:13: 12000000 INFO @ Sun, 03 Jun 2018 14:38:14: 12000000 INFO @ Sun, 03 Jun 2018 14:38:15: 13000000 INFO @ Sun, 03 Jun 2018 14:38:20: 13000000 INFO @ Sun, 03 Jun 2018 14:38:22: 13000000 INFO @ Sun, 03 Jun 2018 14:38:22: 14000000 INFO @ Sun, 03 Jun 2018 14:38:28: 14000000 INFO @ Sun, 03 Jun 2018 14:38:29: 14000000 INFO @ Sun, 03 Jun 2018 14:38:30: 15000000 INFO @ Sun, 03 Jun 2018 14:38:36: 15000000 INFO @ Sun, 03 Jun 2018 14:38:37: 16000000 INFO @ Sun, 03 Jun 2018 14:38:37: 15000000 INFO @ Sun, 03 Jun 2018 14:38:44: 16000000 INFO @ Sun, 03 Jun 2018 14:38:44: 17000000 INFO @ Sun, 03 Jun 2018 14:38:45: 16000000 INFO @ Sun, 03 Jun 2018 14:38:51: 18000000 INFO @ Sun, 03 Jun 2018 14:38:51: 17000000 INFO @ Sun, 03 Jun 2018 14:38:53: 17000000 INFO @ Sun, 03 Jun 2018 14:38:58: 19000000 INFO @ Sun, 03 Jun 2018 14:38:59: 18000000 INFO @ Sun, 03 Jun 2018 14:39:01: 18000000 INFO @ Sun, 03 Jun 2018 14:39:06: 20000000 INFO @ Sun, 03 Jun 2018 14:39:07: 19000000 INFO @ Sun, 03 Jun 2018 14:39:09: 19000000 INFO @ Sun, 03 Jun 2018 14:39:13: 21000000 INFO @ Sun, 03 Jun 2018 14:39:15: 20000000 INFO @ Sun, 03 Jun 2018 14:39:17: 20000000 INFO @ Sun, 03 Jun 2018 14:39:20: 22000000 INFO @ Sun, 03 Jun 2018 14:39:22: 21000000 INFO @ Sun, 03 Jun 2018 14:39:24: 21000000 INFO @ Sun, 03 Jun 2018 14:39:27: 23000000 INFO @ Sun, 03 Jun 2018 14:39:30: 22000000 INFO @ Sun, 03 Jun 2018 14:39:32: 22000000 INFO @ Sun, 03 Jun 2018 14:39:34: 24000000 INFO @ Sun, 03 Jun 2018 14:39:38: 23000000 INFO @ Sun, 03 Jun 2018 14:39:40: 23000000 INFO @ Sun, 03 Jun 2018 14:39:42: 25000000 INFO @ Sun, 03 Jun 2018 14:39:46: 24000000 INFO @ Sun, 03 Jun 2018 14:39:48: 24000000 INFO @ Sun, 03 Jun 2018 14:39:49: 26000000 INFO @ Sun, 03 Jun 2018 14:39:53: 25000000 INFO @ Sun, 03 Jun 2018 14:39:56: 25000000 INFO @ Sun, 03 Jun 2018 14:39:56: 27000000 INFO @ Sun, 03 Jun 2018 14:40:01: 26000000 INFO @ Sun, 03 Jun 2018 14:40:03: 28000000 INFO @ Sun, 03 Jun 2018 14:40:04: 26000000 INFO @ Sun, 03 Jun 2018 14:40:09: 27000000 INFO @ Sun, 03 Jun 2018 14:40:10: 29000000 INFO @ Sun, 03 Jun 2018 14:40:12: 27000000 INFO @ Sun, 03 Jun 2018 14:40:17: 28000000 INFO @ Sun, 03 Jun 2018 14:40:17: 30000000 INFO @ Sun, 03 Jun 2018 14:40:19: 28000000 INFO @ Sun, 03 Jun 2018 14:40:24: 29000000 INFO @ Sun, 03 Jun 2018 14:40:25: 31000000 INFO @ Sun, 03 Jun 2018 14:40:27: 29000000 INFO @ Sun, 03 Jun 2018 14:40:32: 32000000 INFO @ Sun, 03 Jun 2018 14:40:32: 30000000 INFO @ Sun, 03 Jun 2018 14:40:35: 30000000 INFO @ Sun, 03 Jun 2018 14:40:39: 33000000 INFO @ Sun, 03 Jun 2018 14:40:40: 31000000 INFO @ Sun, 03 Jun 2018 14:40:43: 31000000 INFO @ Sun, 03 Jun 2018 14:40:46: 34000000 INFO @ Sun, 03 Jun 2018 14:40:48: 32000000 INFO @ Sun, 03 Jun 2018 14:40:51: 32000000 INFO @ Sun, 03 Jun 2018 14:40:53: 35000000 INFO @ Sun, 03 Jun 2018 14:40:56: 33000000 INFO @ Sun, 03 Jun 2018 14:40:59: 33000000 INFO @ Sun, 03 Jun 2018 14:41:01: 36000000 INFO @ Sun, 03 Jun 2018 14:41:03: 34000000 INFO @ Sun, 03 Jun 2018 14:41:06: 34000000 INFO @ Sun, 03 Jun 2018 14:41:08: 37000000 INFO @ Sun, 03 Jun 2018 14:41:11: 35000000 INFO @ Sun, 03 Jun 2018 14:41:14: 35000000 INFO @ Sun, 03 Jun 2018 14:41:15: 38000000 INFO @ Sun, 03 Jun 2018 14:41:19: 36000000 INFO @ Sun, 03 Jun 2018 14:41:22: 36000000 INFO @ Sun, 03 Jun 2018 14:41:22: 39000000 INFO @ Sun, 03 Jun 2018 14:41:27: 37000000 INFO @ Sun, 03 Jun 2018 14:41:29: 40000000 INFO @ Sun, 03 Jun 2018 14:41:30: 37000000 INFO @ Sun, 03 Jun 2018 14:41:35: 38000000 INFO @ Sun, 03 Jun 2018 14:41:37: 41000000 INFO @ Sun, 03 Jun 2018 14:41:38: 38000000 INFO @ Sun, 03 Jun 2018 14:41:43: 39000000 INFO @ Sun, 03 Jun 2018 14:41:44: 42000000 INFO @ Sun, 03 Jun 2018 14:41:46: 39000000 INFO @ Sun, 03 Jun 2018 14:41:50: 40000000 INFO @ Sun, 03 Jun 2018 14:41:51: 43000000 INFO @ Sun, 03 Jun 2018 14:41:54: 40000000 INFO @ Sun, 03 Jun 2018 14:41:58: 44000000 INFO @ Sun, 03 Jun 2018 14:41:58: 41000000 INFO @ Sun, 03 Jun 2018 14:42:02: 41000000 INFO @ Sun, 03 Jun 2018 14:42:05: 45000000 INFO @ Sun, 03 Jun 2018 14:42:06: 42000000 INFO @ Sun, 03 Jun 2018 14:42:10: 42000000 INFO @ Sun, 03 Jun 2018 14:42:12: 46000000 INFO @ Sun, 03 Jun 2018 14:42:14: 43000000 INFO @ Sun, 03 Jun 2018 14:42:18: 43000000 INFO @ Sun, 03 Jun 2018 14:42:19: 47000000 INFO @ Sun, 03 Jun 2018 14:42:22: 44000000 INFO @ Sun, 03 Jun 2018 14:42:26: 44000000 INFO @ Sun, 03 Jun 2018 14:42:27: 48000000 INFO @ Sun, 03 Jun 2018 14:42:31: 45000000 INFO @ Sun, 03 Jun 2018 14:42:34: 49000000 INFO @ Sun, 03 Jun 2018 14:42:35: 45000000 INFO @ Sun, 03 Jun 2018 14:42:40: 46000000 INFO @ Sun, 03 Jun 2018 14:42:41: 50000000 INFO @ Sun, 03 Jun 2018 14:42:44: 46000000 INFO @ Sun, 03 Jun 2018 14:42:48: 51000000 INFO @ Sun, 03 Jun 2018 14:42:49: 47000000 INFO @ Sun, 03 Jun 2018 14:42:53: 47000000 INFO @ Sun, 03 Jun 2018 14:42:56: 52000000 INFO @ Sun, 03 Jun 2018 14:42:57: 48000000 INFO @ Sun, 03 Jun 2018 14:43:02: 48000000 INFO @ Sun, 03 Jun 2018 14:43:03: 53000000 INFO @ Sun, 03 Jun 2018 14:43:06: 49000000 INFO @ Sun, 03 Jun 2018 14:43:11: 54000000 INFO @ Sun, 03 Jun 2018 14:43:11: 49000000 INFO @ Sun, 03 Jun 2018 14:43:15: 50000000 INFO @ Sun, 03 Jun 2018 14:43:19: 55000000 INFO @ Sun, 03 Jun 2018 14:43:20: 50000000 INFO @ Sun, 03 Jun 2018 14:43:25: 51000000 INFO @ Sun, 03 Jun 2018 14:43:26: 56000000 INFO @ Sun, 03 Jun 2018 14:43:30: 51000000 INFO @ Sun, 03 Jun 2018 14:43:33: 57000000 INFO @ Sun, 03 Jun 2018 14:43:34: 52000000 INFO @ Sun, 03 Jun 2018 14:43:39: 52000000 INFO @ Sun, 03 Jun 2018 14:43:41: 58000000 INFO @ Sun, 03 Jun 2018 14:43:43: 53000000 INFO @ Sun, 03 Jun 2018 14:43:48: 59000000 INFO @ Sun, 03 Jun 2018 14:43:48: 53000000 INFO @ Sun, 03 Jun 2018 14:43:52: 54000000 INFO @ Sun, 03 Jun 2018 14:43:56: 60000000 INFO @ Sun, 03 Jun 2018 14:43:58: 54000000 INFO @ Sun, 03 Jun 2018 14:44:01: 55000000 INFO @ Sun, 03 Jun 2018 14:44:03: 61000000 INFO @ Sun, 03 Jun 2018 14:44:08: 55000000 INFO @ Sun, 03 Jun 2018 14:44:10: 62000000 INFO @ Sun, 03 Jun 2018 14:44:11: 56000000 INFO @ Sun, 03 Jun 2018 14:44:17: 56000000 INFO @ Sun, 03 Jun 2018 14:44:18: 63000000 INFO @ Sun, 03 Jun 2018 14:44:20: 57000000 INFO @ Sun, 03 Jun 2018 14:44:25: 64000000 INFO @ Sun, 03 Jun 2018 14:44:27: 57000000 INFO @ Sun, 03 Jun 2018 14:44:29: 58000000 INFO @ Sun, 03 Jun 2018 14:44:33: 65000000 INFO @ Sun, 03 Jun 2018 14:44:37: 58000000 INFO @ Sun, 03 Jun 2018 14:44:38: 59000000 INFO @ Sun, 03 Jun 2018 14:44:40: 66000000 INFO @ Sun, 03 Jun 2018 14:44:44: #1 tag size is determined as 101 bps INFO @ Sun, 03 Jun 2018 14:44:44: #1 tag size = 101 INFO @ Sun, 03 Jun 2018 14:44:44: #1 total tags in treatment: 32900207 INFO @ Sun, 03 Jun 2018 14:44:44: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 14:44:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 14:44:45: #1 tags after filtering in treatment: 28804695 INFO @ Sun, 03 Jun 2018 14:44:45: #1 Redundant rate of treatment: 0.12 INFO @ Sun, 03 Jun 2018 14:44:45: #1 finished! INFO @ Sun, 03 Jun 2018 14:44:45: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 14:44:45: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 14:44:47: #2 number of paired peaks: 123 WARNING @ Sun, 03 Jun 2018 14:44:47: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Sun, 03 Jun 2018 14:44:47: start model_add_line... INFO @ Sun, 03 Jun 2018 14:44:47: 59000000 INFO @ Sun, 03 Jun 2018 14:44:47: start X-correlation... INFO @ Sun, 03 Jun 2018 14:44:47: end of X-cor INFO @ Sun, 03 Jun 2018 14:44:47: #2 finished! INFO @ Sun, 03 Jun 2018 14:44:47: #2 predicted fragment length is 109 bps INFO @ Sun, 03 Jun 2018 14:44:47: #2 alternative fragment length(s) may be 2,109,131,183,200 bps INFO @ Sun, 03 Jun 2018 14:44:47: #2.2 Generate R script for model : SRX2985937.05_model.r WARNING @ Sun, 03 Jun 2018 14:44:47: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 03 Jun 2018 14:44:47: #2 You may need to consider one of the other alternative d(s): 2,109,131,183,200 WARNING @ Sun, 03 Jun 2018 14:44:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 03 Jun 2018 14:44:47: #3 Call peaks... INFO @ Sun, 03 Jun 2018 14:44:47: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 03 Jun 2018 14:44:47: 60000000 INFO @ Sun, 03 Jun 2018 14:44:56: 61000000 INFO @ Sun, 03 Jun 2018 14:44:57: 60000000 INFO @ Sun, 03 Jun 2018 14:45:05: 62000000 INFO @ Sun, 03 Jun 2018 14:45:07: 61000000 INFO @ Sun, 03 Jun 2018 14:45:15: 63000000 INFO @ Sun, 03 Jun 2018 14:45:16: 62000000 INFO @ Sun, 03 Jun 2018 14:45:24: 64000000 INFO @ Sun, 03 Jun 2018 14:45:25: 63000000 INFO @ Sun, 03 Jun 2018 14:45:33: 65000000 INFO @ Sun, 03 Jun 2018 14:45:34: 64000000 INFO @ Sun, 03 Jun 2018 14:45:36: #3 Call peaks for each chromosome... INFO @ Sun, 03 Jun 2018 14:45:42: 66000000 INFO @ Sun, 03 Jun 2018 14:45:43: 65000000 INFO @ Sun, 03 Jun 2018 14:45:47: #1 tag size is determined as 101 bps INFO @ Sun, 03 Jun 2018 14:45:47: #1 tag size = 101 INFO @ Sun, 03 Jun 2018 14:45:47: #1 total tags in treatment: 32900207 INFO @ Sun, 03 Jun 2018 14:45:47: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 14:45:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 14:45:48: #1 tags after filtering in treatment: 28804695 INFO @ Sun, 03 Jun 2018 14:45:48: #1 Redundant rate of treatment: 0.12 INFO @ Sun, 03 Jun 2018 14:45:48: #1 finished! INFO @ Sun, 03 Jun 2018 14:45:48: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 14:45:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 14:45:50: #2 number of paired peaks: 123 WARNING @ Sun, 03 Jun 2018 14:45:50: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Sun, 03 Jun 2018 14:45:50: start model_add_line... INFO @ Sun, 03 Jun 2018 14:45:50: start X-correlation... INFO @ Sun, 03 Jun 2018 14:45:50: end of X-cor INFO @ Sun, 03 Jun 2018 14:45:50: #2 finished! INFO @ Sun, 03 Jun 2018 14:45:50: #2 predicted fragment length is 109 bps INFO @ Sun, 03 Jun 2018 14:45:50: #2 alternative fragment length(s) may be 2,109,131,183,200 bps INFO @ Sun, 03 Jun 2018 14:45:50: #2.2 Generate R script for model : SRX2985937.20_model.r WARNING @ Sun, 03 Jun 2018 14:45:50: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 03 Jun 2018 14:45:50: #2 You may need to consider one of the other alternative d(s): 2,109,131,183,200 WARNING @ Sun, 03 Jun 2018 14:45:50: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 03 Jun 2018 14:45:50: #3 Call peaks... INFO @ Sun, 03 Jun 2018 14:45:50: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 03 Jun 2018 14:45:52: 66000000 INFO @ Sun, 03 Jun 2018 14:45:56: #1 tag size is determined as 101 bps INFO @ Sun, 03 Jun 2018 14:45:56: #1 tag size = 101 INFO @ Sun, 03 Jun 2018 14:45:56: #1 total tags in treatment: 32900207 INFO @ Sun, 03 Jun 2018 14:45:56: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 14:45:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 14:45:57: #1 tags after filtering in treatment: 28804695 INFO @ Sun, 03 Jun 2018 14:45:57: #1 Redundant rate of treatment: 0.12 INFO @ Sun, 03 Jun 2018 14:45:57: #1 finished! INFO @ Sun, 03 Jun 2018 14:45:57: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 14:45:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 14:45:59: #4 Write output xls file... SRX2985937.05_peaks.xls INFO @ Sun, 03 Jun 2018 14:45:59: #4 Write peak in narrowPeak format file... SRX2985937.05_peaks.narrowPeak INFO @ Sun, 03 Jun 2018 14:45:59: #4 Write summits bed file... SRX2985937.05_summits.bed INFO @ Sun, 03 Jun 2018 14:45:59: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (602 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 03 Jun 2018 14:45:59: #2 number of paired peaks: 123 WARNING @ Sun, 03 Jun 2018 14:45:59: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Sun, 03 Jun 2018 14:45:59: start model_add_line... INFO @ Sun, 03 Jun 2018 14:45:59: start X-correlation... INFO @ Sun, 03 Jun 2018 14:45:59: end of X-cor INFO @ Sun, 03 Jun 2018 14:45:59: #2 finished! INFO @ Sun, 03 Jun 2018 14:45:59: #2 predicted fragment length is 109 bps INFO @ Sun, 03 Jun 2018 14:45:59: #2 alternative fragment length(s) may be 2,109,131,183,200 bps INFO @ Sun, 03 Jun 2018 14:45:59: #2.2 Generate R script for model : SRX2985937.10_model.r WARNING @ Sun, 03 Jun 2018 14:45:59: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 03 Jun 2018 14:45:59: #2 You may need to consider one of the other alternative d(s): 2,109,131,183,200 WARNING @ Sun, 03 Jun 2018 14:45:59: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 03 Jun 2018 14:45:59: #3 Call peaks... INFO @ Sun, 03 Jun 2018 14:45:59: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 03 Jun 2018 14:46:40: #3 Call peaks for each chromosome... INFO @ Sun, 03 Jun 2018 14:46:46: #3 Call peaks for each chromosome... INFO @ Sun, 03 Jun 2018 14:47:05: #4 Write output xls file... SRX2985937.20_peaks.xls INFO @ Sun, 03 Jun 2018 14:47:05: #4 Write peak in narrowPeak format file... SRX2985937.20_peaks.narrowPeak INFO @ Sun, 03 Jun 2018 14:47:05: #4 Write summits bed file... SRX2985937.20_summits.bed INFO @ Sun, 03 Jun 2018 14:47:05: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (245 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sun, 03 Jun 2018 14:47:11: #4 Write output xls file... SRX2985937.10_peaks.xls INFO @ Sun, 03 Jun 2018 14:47:11: #4 Write peak in narrowPeak format file... SRX2985937.10_peaks.narrowPeak INFO @ Sun, 03 Jun 2018 14:47:11: #4 Write summits bed file... SRX2985937.10_summits.bed INFO @ Sun, 03 Jun 2018 14:47:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (421 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。