Job ID = 1292013 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 27,403,119 reads read : 54,806,238 reads written : 54,806,238 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:38 27403119 reads; of these: 27403119 (100.00%) were paired; of these: 1014024 (3.70%) aligned concordantly 0 times 22626598 (82.57%) aligned concordantly exactly 1 time 3762497 (13.73%) aligned concordantly >1 times ---- 1014024 pairs aligned concordantly 0 times; of these: 519913 (51.27%) aligned discordantly 1 time ---- 494111 pairs aligned 0 times concordantly or discordantly; of these: 988222 mates make up the pairs; of these: 614905 (62.22%) aligned 0 times 218801 (22.14%) aligned exactly 1 time 154516 (15.64%) aligned >1 times 98.88% overall alignment rate Time searching: 00:29:38 Overall time: 00:29:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 427317 / 26891665 = 0.0159 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 18:12:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 18:12:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 18:12:57: #1 read tag files... INFO @ Sun, 02 Jun 2019 18:12:57: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 18:12:57: #1 read tag files... INFO @ Sun, 02 Jun 2019 18:12:57: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 18:12:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 18:12:57: #1 read tag files... INFO @ Sun, 02 Jun 2019 18:12:57: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 18:13:05: 1000000 INFO @ Sun, 02 Jun 2019 18:13:05: 1000000 INFO @ Sun, 02 Jun 2019 18:13:08: 1000000 INFO @ Sun, 02 Jun 2019 18:13:12: 2000000 INFO @ Sun, 02 Jun 2019 18:13:12: 2000000 INFO @ Sun, 02 Jun 2019 18:13:18: 2000000 INFO @ Sun, 02 Jun 2019 18:13:20: 3000000 INFO @ Sun, 02 Jun 2019 18:13:20: 3000000 INFO @ Sun, 02 Jun 2019 18:13:28: 4000000 INFO @ Sun, 02 Jun 2019 18:13:28: 4000000 INFO @ Sun, 02 Jun 2019 18:13:28: 3000000 INFO @ Sun, 02 Jun 2019 18:13:35: 5000000 INFO @ Sun, 02 Jun 2019 18:13:35: 5000000 INFO @ Sun, 02 Jun 2019 18:13:38: 4000000 INFO @ Sun, 02 Jun 2019 18:13:43: 6000000 INFO @ Sun, 02 Jun 2019 18:13:43: 6000000 INFO @ Sun, 02 Jun 2019 18:13:49: 5000000 INFO @ Sun, 02 Jun 2019 18:13:50: 7000000 INFO @ Sun, 02 Jun 2019 18:13:50: 7000000 INFO @ Sun, 02 Jun 2019 18:13:58: 8000000 INFO @ Sun, 02 Jun 2019 18:13:58: 8000000 INFO @ Sun, 02 Jun 2019 18:13:59: 6000000 INFO @ Sun, 02 Jun 2019 18:14:06: 9000000 INFO @ Sun, 02 Jun 2019 18:14:06: 9000000 INFO @ Sun, 02 Jun 2019 18:14:09: 7000000 INFO @ Sun, 02 Jun 2019 18:14:13: 10000000 INFO @ Sun, 02 Jun 2019 18:14:13: 10000000 INFO @ Sun, 02 Jun 2019 18:14:20: 8000000 INFO @ Sun, 02 Jun 2019 18:14:21: 11000000 INFO @ Sun, 02 Jun 2019 18:14:21: 11000000 INFO @ Sun, 02 Jun 2019 18:14:29: 12000000 INFO @ Sun, 02 Jun 2019 18:14:29: 12000000 INFO @ Sun, 02 Jun 2019 18:14:30: 9000000 INFO @ Sun, 02 Jun 2019 18:14:36: 13000000 INFO @ Sun, 02 Jun 2019 18:14:36: 13000000 INFO @ Sun, 02 Jun 2019 18:14:40: 10000000 INFO @ Sun, 02 Jun 2019 18:14:44: 14000000 INFO @ Sun, 02 Jun 2019 18:14:44: 14000000 INFO @ Sun, 02 Jun 2019 18:14:51: 11000000 INFO @ Sun, 02 Jun 2019 18:14:51: 15000000 INFO @ Sun, 02 Jun 2019 18:14:51: 15000000 INFO @ Sun, 02 Jun 2019 18:14:59: 16000000 INFO @ Sun, 02 Jun 2019 18:14:59: 16000000 INFO @ Sun, 02 Jun 2019 18:15:01: 12000000 INFO @ Sun, 02 Jun 2019 18:15:07: 17000000 INFO @ Sun, 02 Jun 2019 18:15:07: 17000000 INFO @ Sun, 02 Jun 2019 18:15:11: 13000000 INFO @ Sun, 02 Jun 2019 18:15:14: 18000000 INFO @ Sun, 02 Jun 2019 18:15:14: 18000000 INFO @ Sun, 02 Jun 2019 18:15:21: 14000000 INFO @ Sun, 02 Jun 2019 18:15:22: 19000000 INFO @ Sun, 02 Jun 2019 18:15:22: 19000000 INFO @ Sun, 02 Jun 2019 18:15:29: 20000000 INFO @ Sun, 02 Jun 2019 18:15:29: 20000000 INFO @ Sun, 02 Jun 2019 18:15:31: 15000000 INFO @ Sun, 02 Jun 2019 18:15:37: 21000000 INFO @ Sun, 02 Jun 2019 18:15:37: 21000000 INFO @ Sun, 02 Jun 2019 18:15:41: 16000000 INFO @ Sun, 02 Jun 2019 18:15:44: 22000000 INFO @ Sun, 02 Jun 2019 18:15:44: 22000000 INFO @ Sun, 02 Jun 2019 18:15:52: 17000000 INFO @ Sun, 02 Jun 2019 18:15:52: 23000000 INFO @ Sun, 02 Jun 2019 18:15:52: 23000000 INFO @ Sun, 02 Jun 2019 18:16:00: 24000000 INFO @ Sun, 02 Jun 2019 18:16:00: 24000000 INFO @ Sun, 02 Jun 2019 18:16:02: 18000000 INFO @ Sun, 02 Jun 2019 18:16:07: 25000000 INFO @ Sun, 02 Jun 2019 18:16:07: 25000000 INFO @ Sun, 02 Jun 2019 18:16:12: 19000000 INFO @ Sun, 02 Jun 2019 18:16:15: 26000000 INFO @ Sun, 02 Jun 2019 18:16:15: 26000000 INFO @ Sun, 02 Jun 2019 18:16:22: 20000000 INFO @ Sun, 02 Jun 2019 18:16:22: 27000000 INFO @ Sun, 02 Jun 2019 18:16:22: 27000000 INFO @ Sun, 02 Jun 2019 18:16:29: 28000000 INFO @ Sun, 02 Jun 2019 18:16:29: 28000000 INFO @ Sun, 02 Jun 2019 18:16:32: 21000000 INFO @ Sun, 02 Jun 2019 18:16:37: 29000000 INFO @ Sun, 02 Jun 2019 18:16:37: 29000000 INFO @ Sun, 02 Jun 2019 18:16:41: 22000000 INFO @ Sun, 02 Jun 2019 18:16:44: 30000000 INFO @ Sun, 02 Jun 2019 18:16:44: 30000000 INFO @ Sun, 02 Jun 2019 18:16:51: 23000000 INFO @ Sun, 02 Jun 2019 18:16:52: 31000000 INFO @ Sun, 02 Jun 2019 18:16:52: 31000000 INFO @ Sun, 02 Jun 2019 18:16:59: 32000000 INFO @ Sun, 02 Jun 2019 18:16:59: 32000000 INFO @ Sun, 02 Jun 2019 18:17:01: 24000000 INFO @ Sun, 02 Jun 2019 18:17:06: 33000000 INFO @ Sun, 02 Jun 2019 18:17:06: 33000000 INFO @ Sun, 02 Jun 2019 18:17:11: 25000000 INFO @ Sun, 02 Jun 2019 18:17:14: 34000000 INFO @ Sun, 02 Jun 2019 18:17:14: 34000000 INFO @ Sun, 02 Jun 2019 18:17:21: 35000000 INFO @ Sun, 02 Jun 2019 18:17:21: 35000000 INFO @ Sun, 02 Jun 2019 18:17:21: 26000000 INFO @ Sun, 02 Jun 2019 18:17:29: 36000000 INFO @ Sun, 02 Jun 2019 18:17:29: 36000000 INFO @ Sun, 02 Jun 2019 18:17:31: 27000000 INFO @ Sun, 02 Jun 2019 18:17:36: 37000000 INFO @ Sun, 02 Jun 2019 18:17:36: 37000000 INFO @ Sun, 02 Jun 2019 18:17:40: 28000000 INFO @ Sun, 02 Jun 2019 18:17:44: 38000000 INFO @ Sun, 02 Jun 2019 18:17:44: 38000000 INFO @ Sun, 02 Jun 2019 18:17:50: 29000000 INFO @ Sun, 02 Jun 2019 18:17:51: 39000000 INFO @ Sun, 02 Jun 2019 18:17:51: 39000000 INFO @ Sun, 02 Jun 2019 18:17:58: 40000000 INFO @ Sun, 02 Jun 2019 18:17:58: 40000000 INFO @ Sun, 02 Jun 2019 18:18:00: 30000000 INFO @ Sun, 02 Jun 2019 18:18:06: 41000000 INFO @ Sun, 02 Jun 2019 18:18:06: 41000000 INFO @ Sun, 02 Jun 2019 18:18:11: 31000000 INFO @ Sun, 02 Jun 2019 18:18:13: 42000000 INFO @ Sun, 02 Jun 2019 18:18:13: 42000000 INFO @ Sun, 02 Jun 2019 18:18:21: 43000000 INFO @ Sun, 02 Jun 2019 18:18:21: 43000000 INFO @ Sun, 02 Jun 2019 18:18:21: 32000000 INFO @ Sun, 02 Jun 2019 18:18:28: 44000000 INFO @ Sun, 02 Jun 2019 18:18:28: 44000000 INFO @ Sun, 02 Jun 2019 18:18:31: 33000000 INFO @ Sun, 02 Jun 2019 18:18:35: 45000000 INFO @ Sun, 02 Jun 2019 18:18:35: 45000000 INFO @ Sun, 02 Jun 2019 18:18:41: 34000000 INFO @ Sun, 02 Jun 2019 18:18:43: 46000000 INFO @ Sun, 02 Jun 2019 18:18:43: 46000000 INFO @ Sun, 02 Jun 2019 18:18:50: 47000000 INFO @ Sun, 02 Jun 2019 18:18:50: 47000000 INFO @ Sun, 02 Jun 2019 18:18:52: 35000000 INFO @ Sun, 02 Jun 2019 18:18:57: 48000000 INFO @ Sun, 02 Jun 2019 18:18:57: 48000000 INFO @ Sun, 02 Jun 2019 18:19:02: 36000000 INFO @ Sun, 02 Jun 2019 18:19:05: 49000000 INFO @ Sun, 02 Jun 2019 18:19:05: 49000000 INFO @ Sun, 02 Jun 2019 18:19:12: 50000000 INFO @ Sun, 02 Jun 2019 18:19:12: 50000000 INFO @ Sun, 02 Jun 2019 18:19:12: 37000000 INFO @ Sun, 02 Jun 2019 18:19:19: 51000000 INFO @ Sun, 02 Jun 2019 18:19:19: 51000000 INFO @ Sun, 02 Jun 2019 18:19:23: 38000000 INFO @ Sun, 02 Jun 2019 18:19:27: 52000000 INFO @ Sun, 02 Jun 2019 18:19:27: 52000000 INFO @ Sun, 02 Jun 2019 18:19:33: 39000000 INFO @ Sun, 02 Jun 2019 18:19:34: 53000000 INFO @ Sun, 02 Jun 2019 18:19:34: 53000000 INFO @ Sun, 02 Jun 2019 18:19:37: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 18:19:37: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 18:19:37: #1 total tags in treatment: 25964768 INFO @ Sun, 02 Jun 2019 18:19:37: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 18:19:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 18:19:37: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 18:19:37: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 18:19:37: #1 total tags in treatment: 25964768 INFO @ Sun, 02 Jun 2019 18:19:37: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 18:19:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 18:19:37: #1 tags after filtering in treatment: 22870698 INFO @ Sun, 02 Jun 2019 18:19:37: #1 Redundant rate of treatment: 0.12 INFO @ Sun, 02 Jun 2019 18:19:37: #1 finished! INFO @ Sun, 02 Jun 2019 18:19:37: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 18:19:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 18:19:37: #1 tags after filtering in treatment: 22870698 INFO @ Sun, 02 Jun 2019 18:19:37: #1 Redundant rate of treatment: 0.12 INFO @ Sun, 02 Jun 2019 18:19:37: #1 finished! INFO @ Sun, 02 Jun 2019 18:19:37: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 18:19:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 18:19:39: #2 number of paired peaks: 200 WARNING @ Sun, 02 Jun 2019 18:19:39: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Sun, 02 Jun 2019 18:19:39: start model_add_line... INFO @ Sun, 02 Jun 2019 18:19:39: #2 number of paired peaks: 200 WARNING @ Sun, 02 Jun 2019 18:19:39: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Sun, 02 Jun 2019 18:19:39: start model_add_line... INFO @ Sun, 02 Jun 2019 18:19:40: start X-correlation... INFO @ Sun, 02 Jun 2019 18:19:40: end of X-cor INFO @ Sun, 02 Jun 2019 18:19:40: #2 finished! INFO @ Sun, 02 Jun 2019 18:19:40: #2 predicted fragment length is 86 bps INFO @ Sun, 02 Jun 2019 18:19:40: #2 alternative fragment length(s) may be 2,86,104 bps INFO @ Sun, 02 Jun 2019 18:19:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.20_model.r WARNING @ Sun, 02 Jun 2019 18:19:40: #2 Since the d (86) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 18:19:40: #2 You may need to consider one of the other alternative d(s): 2,86,104 WARNING @ Sun, 02 Jun 2019 18:19:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 18:19:40: #3 Call peaks... INFO @ Sun, 02 Jun 2019 18:19:40: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 18:19:40: start X-correlation... INFO @ Sun, 02 Jun 2019 18:19:40: end of X-cor INFO @ Sun, 02 Jun 2019 18:19:40: #2 finished! INFO @ Sun, 02 Jun 2019 18:19:40: #2 predicted fragment length is 86 bps INFO @ Sun, 02 Jun 2019 18:19:40: #2 alternative fragment length(s) may be 2,86,104 bps INFO @ Sun, 02 Jun 2019 18:19:40: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.05_model.r WARNING @ Sun, 02 Jun 2019 18:19:40: #2 Since the d (86) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 18:19:40: #2 You may need to consider one of the other alternative d(s): 2,86,104 WARNING @ Sun, 02 Jun 2019 18:19:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 18:19:40: #3 Call peaks... INFO @ Sun, 02 Jun 2019 18:19:40: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 18:19:42: 40000000 INFO @ Sun, 02 Jun 2019 18:19:52: 41000000 INFO @ Sun, 02 Jun 2019 18:20:01: 42000000 INFO @ Sun, 02 Jun 2019 18:20:11: 43000000 INFO @ Sun, 02 Jun 2019 18:20:20: 44000000 INFO @ Sun, 02 Jun 2019 18:20:30: 45000000 INFO @ Sun, 02 Jun 2019 18:20:30: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 18:20:30: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 18:20:39: 46000000 INFO @ Sun, 02 Jun 2019 18:20:48: 47000000 INFO @ Sun, 02 Jun 2019 18:20:53: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.20_peaks.xls INFO @ Sun, 02 Jun 2019 18:20:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 18:20:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.20_summits.bed INFO @ Sun, 02 Jun 2019 18:20:53: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (204 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 18:20:53: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.05_peaks.xls INFO @ Sun, 02 Jun 2019 18:20:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 18:20:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.05_summits.bed INFO @ Sun, 02 Jun 2019 18:20:53: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (551 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 18:20:58: 48000000 INFO @ Sun, 02 Jun 2019 18:21:07: 49000000 INFO @ Sun, 02 Jun 2019 18:21:16: 50000000 INFO @ Sun, 02 Jun 2019 18:21:26: 51000000 INFO @ Sun, 02 Jun 2019 18:21:35: 52000000 INFO @ Sun, 02 Jun 2019 18:21:44: 53000000 INFO @ Sun, 02 Jun 2019 18:21:48: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 18:21:48: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 18:21:48: #1 total tags in treatment: 25964768 INFO @ Sun, 02 Jun 2019 18:21:48: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 18:21:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 18:21:48: #1 tags after filtering in treatment: 22870698 INFO @ Sun, 02 Jun 2019 18:21:48: #1 Redundant rate of treatment: 0.12 INFO @ Sun, 02 Jun 2019 18:21:48: #1 finished! INFO @ Sun, 02 Jun 2019 18:21:48: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 18:21:48: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 18:21:50: #2 number of paired peaks: 200 WARNING @ Sun, 02 Jun 2019 18:21:50: Fewer paired peaks (200) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 200 pairs to build model! INFO @ Sun, 02 Jun 2019 18:21:50: start model_add_line... INFO @ Sun, 02 Jun 2019 18:21:50: start X-correlation... INFO @ Sun, 02 Jun 2019 18:21:50: end of X-cor INFO @ Sun, 02 Jun 2019 18:21:50: #2 finished! INFO @ Sun, 02 Jun 2019 18:21:50: #2 predicted fragment length is 86 bps INFO @ Sun, 02 Jun 2019 18:21:50: #2 alternative fragment length(s) may be 2,86,104 bps INFO @ Sun, 02 Jun 2019 18:21:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.10_model.r WARNING @ Sun, 02 Jun 2019 18:21:50: #2 Since the d (86) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 18:21:50: #2 You may need to consider one of the other alternative d(s): 2,86,104 WARNING @ Sun, 02 Jun 2019 18:21:50: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 18:21:50: #3 Call peaks... INFO @ Sun, 02 Jun 2019 18:21:50: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 18:22:41: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 18:23:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.10_peaks.xls INFO @ Sun, 02 Jun 2019 18:23:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 18:23:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX2978709/SRX2978709.10_summits.bed INFO @ Sun, 02 Jun 2019 18:23:04: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (387 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。