Job ID = 11170823 sra ファイルのダウンロード中... Completed: 1899410K bytes transferred in 217 seconds (71617K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 70079354 spots for /home/okishinya/chipatlas/results/ce10/SRX2973399/SRR5786970.sra Written 70079354 spots for /home/okishinya/chipatlas/results/ce10/SRX2973399/SRR5786970.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:26:13 70079354 reads; of these: 70079354 (100.00%) were unpaired; of these: 11799324 (16.84%) aligned 0 times 48415087 (69.09%) aligned exactly 1 time 9864943 (14.08%) aligned >1 times 83.16% overall alignment rate Time searching: 00:26:13 Overall time: 00:26:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 20370364 / 58280030 = 0.3495 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 12:11:44: # Command line: callpeak -t SRX2973399.bam -f BAM -g ce -n SRX2973399.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2973399.10 # format = BAM # ChIP-seq file = ['SRX2973399.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:11:44: # Command line: callpeak -t SRX2973399.bam -f BAM -g ce -n SRX2973399.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2973399.05 # format = BAM # ChIP-seq file = ['SRX2973399.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:11:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:11:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:11:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:11:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:11:44: # Command line: callpeak -t SRX2973399.bam -f BAM -g ce -n SRX2973399.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2973399.20 # format = BAM # ChIP-seq file = ['SRX2973399.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 12:11:44: #1 read tag files... INFO @ Sat, 08 Sep 2018 12:11:44: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 12:11:52: 1000000 INFO @ Sat, 08 Sep 2018 12:11:52: 1000000 INFO @ Sat, 08 Sep 2018 12:11:52: 1000000 INFO @ Sat, 08 Sep 2018 12:11:59: 2000000 INFO @ Sat, 08 Sep 2018 12:11:59: 2000000 INFO @ Sat, 08 Sep 2018 12:11:59: 2000000 INFO @ Sat, 08 Sep 2018 12:12:06: 3000000 INFO @ Sat, 08 Sep 2018 12:12:07: 3000000 INFO @ Sat, 08 Sep 2018 12:12:07: 3000000 INFO @ Sat, 08 Sep 2018 12:12:14: 4000000 INFO @ Sat, 08 Sep 2018 12:12:15: 4000000 INFO @ Sat, 08 Sep 2018 12:12:15: 4000000 INFO @ Sat, 08 Sep 2018 12:12:21: 5000000 INFO @ Sat, 08 Sep 2018 12:12:22: 5000000 INFO @ Sat, 08 Sep 2018 12:12:22: 5000000 INFO @ Sat, 08 Sep 2018 12:12:28: 6000000 INFO @ Sat, 08 Sep 2018 12:12:30: 6000000 INFO @ Sat, 08 Sep 2018 12:12:30: 6000000 INFO @ Sat, 08 Sep 2018 12:12:36: 7000000 INFO @ Sat, 08 Sep 2018 12:12:37: 7000000 INFO @ Sat, 08 Sep 2018 12:12:37: 7000000 INFO @ Sat, 08 Sep 2018 12:12:43: 8000000 INFO @ Sat, 08 Sep 2018 12:12:45: 8000000 INFO @ Sat, 08 Sep 2018 12:12:45: 8000000 INFO @ Sat, 08 Sep 2018 12:12:50: 9000000 INFO @ Sat, 08 Sep 2018 12:12:52: 9000000 INFO @ Sat, 08 Sep 2018 12:12:52: 9000000 INFO @ Sat, 08 Sep 2018 12:12:57: 10000000 INFO @ Sat, 08 Sep 2018 12:13:00: 10000000 INFO @ Sat, 08 Sep 2018 12:13:00: 10000000 INFO @ Sat, 08 Sep 2018 12:13:05: 11000000 INFO @ Sat, 08 Sep 2018 12:13:07: 11000000 INFO @ Sat, 08 Sep 2018 12:13:07: 11000000 INFO @ Sat, 08 Sep 2018 12:13:12: 12000000 INFO @ Sat, 08 Sep 2018 12:13:15: 12000000 INFO @ Sat, 08 Sep 2018 12:13:15: 12000000 INFO @ Sat, 08 Sep 2018 12:13:19: 13000000 INFO @ Sat, 08 Sep 2018 12:13:23: 13000000 INFO @ Sat, 08 Sep 2018 12:13:23: 13000000 INFO @ Sat, 08 Sep 2018 12:13:26: 14000000 INFO @ Sat, 08 Sep 2018 12:13:31: 14000000 INFO @ Sat, 08 Sep 2018 12:13:31: 14000000 INFO @ Sat, 08 Sep 2018 12:13:33: 15000000 INFO @ Sat, 08 Sep 2018 12:13:39: 15000000 INFO @ Sat, 08 Sep 2018 12:13:39: 15000000 INFO @ Sat, 08 Sep 2018 12:13:40: 16000000 INFO @ Sat, 08 Sep 2018 12:13:47: 16000000 INFO @ Sat, 08 Sep 2018 12:13:47: 16000000 INFO @ Sat, 08 Sep 2018 12:13:47: 17000000 INFO @ Sat, 08 Sep 2018 12:13:54: 18000000 INFO @ Sat, 08 Sep 2018 12:13:54: 17000000 INFO @ Sat, 08 Sep 2018 12:13:54: 17000000 INFO @ Sat, 08 Sep 2018 12:14:01: 19000000 INFO @ Sat, 08 Sep 2018 12:14:02: 18000000 INFO @ Sat, 08 Sep 2018 12:14:02: 18000000 INFO @ Sat, 08 Sep 2018 12:14:08: 20000000 INFO @ Sat, 08 Sep 2018 12:14:10: 19000000 INFO @ Sat, 08 Sep 2018 12:14:10: 19000000 INFO @ Sat, 08 Sep 2018 12:14:15: 21000000 INFO @ Sat, 08 Sep 2018 12:14:18: 20000000 INFO @ Sat, 08 Sep 2018 12:14:18: 20000000 INFO @ Sat, 08 Sep 2018 12:14:22: 22000000 INFO @ Sat, 08 Sep 2018 12:14:26: 21000000 INFO @ Sat, 08 Sep 2018 12:14:26: 21000000 INFO @ Sat, 08 Sep 2018 12:14:29: 23000000 INFO @ Sat, 08 Sep 2018 12:14:34: 22000000 INFO @ Sat, 08 Sep 2018 12:14:34: 22000000 INFO @ Sat, 08 Sep 2018 12:14:37: 24000000 INFO @ Sat, 08 Sep 2018 12:14:42: 23000000 INFO @ Sat, 08 Sep 2018 12:14:42: 23000000 INFO @ Sat, 08 Sep 2018 12:14:44: 25000000 INFO @ Sat, 08 Sep 2018 12:14:49: 24000000 INFO @ Sat, 08 Sep 2018 12:14:49: 24000000 INFO @ Sat, 08 Sep 2018 12:14:51: 26000000 INFO @ Sat, 08 Sep 2018 12:14:57: 25000000 INFO @ Sat, 08 Sep 2018 12:14:57: 25000000 INFO @ Sat, 08 Sep 2018 12:14:58: 27000000 INFO @ Sat, 08 Sep 2018 12:15:05: 28000000 INFO @ Sat, 08 Sep 2018 12:15:05: 26000000 INFO @ Sat, 08 Sep 2018 12:15:05: 26000000 INFO @ Sat, 08 Sep 2018 12:15:12: 29000000 INFO @ Sat, 08 Sep 2018 12:15:13: 27000000 INFO @ Sat, 08 Sep 2018 12:15:13: 27000000 INFO @ Sat, 08 Sep 2018 12:15:19: 30000000 INFO @ Sat, 08 Sep 2018 12:15:21: 28000000 INFO @ Sat, 08 Sep 2018 12:15:21: 28000000 INFO @ Sat, 08 Sep 2018 12:15:26: 31000000 INFO @ Sat, 08 Sep 2018 12:15:29: 29000000 INFO @ Sat, 08 Sep 2018 12:15:29: 29000000 INFO @ Sat, 08 Sep 2018 12:15:33: 32000000 INFO @ Sat, 08 Sep 2018 12:15:36: 30000000 INFO @ Sat, 08 Sep 2018 12:15:36: 30000000 INFO @ Sat, 08 Sep 2018 12:15:40: 33000000 INFO @ Sat, 08 Sep 2018 12:15:44: 31000000 INFO @ Sat, 08 Sep 2018 12:15:44: 31000000 INFO @ Sat, 08 Sep 2018 12:15:47: 34000000 INFO @ Sat, 08 Sep 2018 12:15:52: 32000000 INFO @ Sat, 08 Sep 2018 12:15:52: 32000000 INFO @ Sat, 08 Sep 2018 12:15:54: 35000000 INFO @ Sat, 08 Sep 2018 12:16:00: 33000000 INFO @ Sat, 08 Sep 2018 12:16:00: 33000000 INFO @ Sat, 08 Sep 2018 12:16:01: 36000000 INFO @ Sat, 08 Sep 2018 12:16:08: 37000000 INFO @ Sat, 08 Sep 2018 12:16:08: 34000000 INFO @ Sat, 08 Sep 2018 12:16:08: 34000000 INFO @ Sat, 08 Sep 2018 12:16:14: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:16:14: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:16:14: #1 total tags in treatment: 37909666 INFO @ Sat, 08 Sep 2018 12:16:14: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:16:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:16:15: #1 tags after filtering in treatment: 37909666 INFO @ Sat, 08 Sep 2018 12:16:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:16:15: #1 finished! INFO @ Sat, 08 Sep 2018 12:16:15: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:16:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:16:16: 35000000 INFO @ Sat, 08 Sep 2018 12:16:16: 35000000 INFO @ Sat, 08 Sep 2018 12:16:17: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 12:16:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:16:17: Process for pairing-model is terminated! cat: SRX2973399.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 17 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973399.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973399.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973399.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 12:16:24: 36000000 INFO @ Sat, 08 Sep 2018 12:16:24: 36000000 INFO @ Sat, 08 Sep 2018 12:16:32: 37000000 INFO @ Sat, 08 Sep 2018 12:16:32: 37000000 INFO @ Sat, 08 Sep 2018 12:16:39: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:16:39: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:16:39: #1 total tags in treatment: 37909666 INFO @ Sat, 08 Sep 2018 12:16:39: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:16:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:16:39: #1 tag size is determined as 76 bps INFO @ Sat, 08 Sep 2018 12:16:39: #1 tag size = 76 INFO @ Sat, 08 Sep 2018 12:16:39: #1 total tags in treatment: 37909666 INFO @ Sat, 08 Sep 2018 12:16:39: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 12:16:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 12:16:40: #1 tags after filtering in treatment: 37909666 INFO @ Sat, 08 Sep 2018 12:16:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:16:40: #1 finished! INFO @ Sat, 08 Sep 2018 12:16:40: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:16:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:16:40: #1 tags after filtering in treatment: 37909666 INFO @ Sat, 08 Sep 2018 12:16:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 12:16:40: #1 finished! INFO @ Sat, 08 Sep 2018 12:16:40: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 12:16:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 12:16:42: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 12:16:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:16:42: Process for pairing-model is terminated! INFO @ Sat, 08 Sep 2018 12:16:42: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 12:16:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 12:16:42: Process for pairing-model is terminated! cat: SRX2973399.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2973399.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973399.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973399.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973399.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973399.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973399.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973399.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。