Job ID = 10845152 sra ファイルのダウンロード中... Completed: 2322175K bytes transferred in 137 seconds (138550K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 45310834 spots for /home/okishinya/chipatlas/results/ce10/SRX2973389/SRR5786980.sra Written 45310834 spots for /home/okishinya/chipatlas/results/ce10/SRX2973389/SRR5786980.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:19 45310834 reads; of these: 45310834 (100.00%) were unpaired; of these: 9131210 (20.15%) aligned 0 times 31241194 (68.95%) aligned exactly 1 time 4938430 (10.90%) aligned >1 times 79.85% overall alignment rate Time searching: 00:20:19 Overall time: 00:20:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6697037 / 36179624 = 0.1851 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 04 Jul 2018 10:02:56: # Command line: callpeak -t SRX2973389.bam -f BAM -g ce -n SRX2973389.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2973389.05 # format = BAM # ChIP-seq file = ['SRX2973389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 10:02:56: #1 read tag files... INFO @ Wed, 04 Jul 2018 10:02:56: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 10:02:56: # Command line: callpeak -t SRX2973389.bam -f BAM -g ce -n SRX2973389.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2973389.20 # format = BAM # ChIP-seq file = ['SRX2973389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 10:02:56: #1 read tag files... INFO @ Wed, 04 Jul 2018 10:02:56: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 10:02:56: # Command line: callpeak -t SRX2973389.bam -f BAM -g ce -n SRX2973389.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2973389.10 # format = BAM # ChIP-seq file = ['SRX2973389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 10:02:56: #1 read tag files... INFO @ Wed, 04 Jul 2018 10:02:56: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 10:03:06: 1000000 INFO @ Wed, 04 Jul 2018 10:03:07: 1000000 INFO @ Wed, 04 Jul 2018 10:03:07: 1000000 INFO @ Wed, 04 Jul 2018 10:03:16: 2000000 INFO @ Wed, 04 Jul 2018 10:03:18: 2000000 INFO @ Wed, 04 Jul 2018 10:03:19: 2000000 INFO @ Wed, 04 Jul 2018 10:03:25: 3000000 INFO @ Wed, 04 Jul 2018 10:03:29: 3000000 INFO @ Wed, 04 Jul 2018 10:03:31: 3000000 INFO @ Wed, 04 Jul 2018 10:03:33: 4000000 INFO @ Wed, 04 Jul 2018 10:03:40: 4000000 INFO @ Wed, 04 Jul 2018 10:03:42: 5000000 INFO @ Wed, 04 Jul 2018 10:03:42: 4000000 INFO @ Wed, 04 Jul 2018 10:03:49: 5000000 INFO @ Wed, 04 Jul 2018 10:03:50: 6000000 INFO @ Wed, 04 Jul 2018 10:03:51: 5000000 INFO @ Wed, 04 Jul 2018 10:03:58: 6000000 INFO @ Wed, 04 Jul 2018 10:03:58: 7000000 INFO @ Wed, 04 Jul 2018 10:04:00: 6000000 INFO @ Wed, 04 Jul 2018 10:04:07: 8000000 INFO @ Wed, 04 Jul 2018 10:04:07: 7000000 INFO @ Wed, 04 Jul 2018 10:04:10: 7000000 INFO @ Wed, 04 Jul 2018 10:04:15: 9000000 INFO @ Wed, 04 Jul 2018 10:04:16: 8000000 INFO @ Wed, 04 Jul 2018 10:04:19: 8000000 INFO @ Wed, 04 Jul 2018 10:04:24: 10000000 INFO @ Wed, 04 Jul 2018 10:04:25: 9000000 INFO @ Wed, 04 Jul 2018 10:04:28: 9000000 INFO @ Wed, 04 Jul 2018 10:04:32: 11000000 INFO @ Wed, 04 Jul 2018 10:04:34: 10000000 INFO @ Wed, 04 Jul 2018 10:04:37: 10000000 INFO @ Wed, 04 Jul 2018 10:04:40: 12000000 INFO @ Wed, 04 Jul 2018 10:04:44: 11000000 INFO @ Wed, 04 Jul 2018 10:04:46: 11000000 INFO @ Wed, 04 Jul 2018 10:04:49: 13000000 INFO @ Wed, 04 Jul 2018 10:04:53: 12000000 INFO @ Wed, 04 Jul 2018 10:04:55: 12000000 INFO @ Wed, 04 Jul 2018 10:04:57: 14000000 INFO @ Wed, 04 Jul 2018 10:05:02: 13000000 INFO @ Wed, 04 Jul 2018 10:05:04: 13000000 INFO @ Wed, 04 Jul 2018 10:05:06: 15000000 INFO @ Wed, 04 Jul 2018 10:05:11: 14000000 INFO @ Wed, 04 Jul 2018 10:05:14: 14000000 INFO @ Wed, 04 Jul 2018 10:05:14: 16000000 INFO @ Wed, 04 Jul 2018 10:05:20: 15000000 INFO @ Wed, 04 Jul 2018 10:05:23: 17000000 INFO @ Wed, 04 Jul 2018 10:05:23: 15000000 INFO @ Wed, 04 Jul 2018 10:05:29: 16000000 INFO @ Wed, 04 Jul 2018 10:05:31: 18000000 INFO @ Wed, 04 Jul 2018 10:05:32: 16000000 INFO @ Wed, 04 Jul 2018 10:05:39: 17000000 INFO @ Wed, 04 Jul 2018 10:05:39: 19000000 INFO @ Wed, 04 Jul 2018 10:05:41: 17000000 INFO @ Wed, 04 Jul 2018 10:05:48: 18000000 INFO @ Wed, 04 Jul 2018 10:05:48: 20000000 INFO @ Wed, 04 Jul 2018 10:05:50: 18000000 INFO @ Wed, 04 Jul 2018 10:05:56: 21000000 INFO @ Wed, 04 Jul 2018 10:05:57: 19000000 INFO @ Wed, 04 Jul 2018 10:06:00: 19000000 INFO @ Wed, 04 Jul 2018 10:06:05: 22000000 INFO @ Wed, 04 Jul 2018 10:06:06: 20000000 INFO @ Wed, 04 Jul 2018 10:06:09: 20000000 INFO @ Wed, 04 Jul 2018 10:06:13: 23000000 INFO @ Wed, 04 Jul 2018 10:06:15: 21000000 INFO @ Wed, 04 Jul 2018 10:06:18: 21000000 INFO @ Wed, 04 Jul 2018 10:06:22: 24000000 INFO @ Wed, 04 Jul 2018 10:06:24: 22000000 INFO @ Wed, 04 Jul 2018 10:06:27: 22000000 INFO @ Wed, 04 Jul 2018 10:06:30: 25000000 INFO @ Wed, 04 Jul 2018 10:06:34: 23000000 INFO @ Wed, 04 Jul 2018 10:06:36: 23000000 INFO @ Wed, 04 Jul 2018 10:06:39: 26000000 INFO @ Wed, 04 Jul 2018 10:06:43: 24000000 INFO @ Wed, 04 Jul 2018 10:06:46: 24000000 INFO @ Wed, 04 Jul 2018 10:06:47: 27000000 INFO @ Wed, 04 Jul 2018 10:06:52: 25000000 INFO @ Wed, 04 Jul 2018 10:06:55: 25000000 INFO @ Wed, 04 Jul 2018 10:06:55: 28000000 INFO @ Wed, 04 Jul 2018 10:07:01: 26000000 INFO @ Wed, 04 Jul 2018 10:07:04: 26000000 INFO @ Wed, 04 Jul 2018 10:07:04: 29000000 INFO @ Wed, 04 Jul 2018 10:07:08: #1 tag size is determined as 101 bps INFO @ Wed, 04 Jul 2018 10:07:08: #1 tag size = 101 INFO @ Wed, 04 Jul 2018 10:07:08: #1 total tags in treatment: 29482587 INFO @ Wed, 04 Jul 2018 10:07:08: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 10:07:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 10:07:09: #1 tags after filtering in treatment: 29482587 INFO @ Wed, 04 Jul 2018 10:07:09: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 10:07:09: #1 finished! INFO @ Wed, 04 Jul 2018 10:07:09: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 10:07:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 10:07:10: 27000000 INFO @ Wed, 04 Jul 2018 10:07:11: #2 number of paired peaks: 47 WARNING @ Wed, 04 Jul 2018 10:07:11: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 04 Jul 2018 10:07:11: Process for pairing-model is terminated! cat: SRX2973389.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973389.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973389.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973389.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 04 Jul 2018 10:07:13: 27000000 INFO @ Wed, 04 Jul 2018 10:07:19: 28000000 INFO @ Wed, 04 Jul 2018 10:07:22: 28000000 INFO @ Wed, 04 Jul 2018 10:07:28: 29000000 INFO @ Wed, 04 Jul 2018 10:07:31: 29000000 INFO @ Wed, 04 Jul 2018 10:07:32: #1 tag size is determined as 101 bps INFO @ Wed, 04 Jul 2018 10:07:32: #1 tag size = 101 INFO @ Wed, 04 Jul 2018 10:07:32: #1 total tags in treatment: 29482587 INFO @ Wed, 04 Jul 2018 10:07:32: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 10:07:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 10:07:33: #1 tags after filtering in treatment: 29482587 INFO @ Wed, 04 Jul 2018 10:07:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 10:07:33: #1 finished! INFO @ Wed, 04 Jul 2018 10:07:33: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 10:07:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 10:07:35: #2 number of paired peaks: 47 WARNING @ Wed, 04 Jul 2018 10:07:35: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 04 Jul 2018 10:07:35: Process for pairing-model is terminated! cat: SRX2973389.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973389.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973389.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973389.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 04 Jul 2018 10:07:35: #1 tag size is determined as 101 bps INFO @ Wed, 04 Jul 2018 10:07:35: #1 tag size = 101 INFO @ Wed, 04 Jul 2018 10:07:35: #1 total tags in treatment: 29482587 INFO @ Wed, 04 Jul 2018 10:07:35: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 10:07:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 10:07:36: #1 tags after filtering in treatment: 29482587 INFO @ Wed, 04 Jul 2018 10:07:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 10:07:36: #1 finished! INFO @ Wed, 04 Jul 2018 10:07:36: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 10:07:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 10:07:38: #2 number of paired peaks: 47 WARNING @ Wed, 04 Jul 2018 10:07:38: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 04 Jul 2018 10:07:38: Process for pairing-model is terminated! cat: SRX2973389.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973389.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973389.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973389.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。