Job ID = 1291878 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 36,121,620 reads read : 72,243,240 reads written : 36,121,620 reads 0-length : 36,121,620 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:13 36121620 reads; of these: 36121620 (100.00%) were unpaired; of these: 703127 (1.95%) aligned 0 times 29695903 (82.21%) aligned exactly 1 time 5722590 (15.84%) aligned >1 times 98.05% overall alignment rate Time searching: 00:09:13 Overall time: 00:09:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5261622 / 35418493 = 0.1486 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:16:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:16:50: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:16:50: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:16:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:16:50: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:16:50: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:16:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:16:50: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:16:50: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:16:56: 1000000 INFO @ Sun, 02 Jun 2019 17:16:58: 1000000 INFO @ Sun, 02 Jun 2019 17:16:59: 1000000 INFO @ Sun, 02 Jun 2019 17:17:03: 2000000 INFO @ Sun, 02 Jun 2019 17:17:04: 2000000 INFO @ Sun, 02 Jun 2019 17:17:07: 2000000 INFO @ Sun, 02 Jun 2019 17:17:09: 3000000 INFO @ Sun, 02 Jun 2019 17:17:11: 3000000 INFO @ Sun, 02 Jun 2019 17:17:15: 4000000 INFO @ Sun, 02 Jun 2019 17:17:16: 3000000 INFO @ Sun, 02 Jun 2019 17:17:18: 4000000 INFO @ Sun, 02 Jun 2019 17:17:22: 5000000 INFO @ Sun, 02 Jun 2019 17:17:24: 4000000 INFO @ Sun, 02 Jun 2019 17:17:25: 5000000 INFO @ Sun, 02 Jun 2019 17:17:28: 6000000 INFO @ Sun, 02 Jun 2019 17:17:32: 5000000 INFO @ Sun, 02 Jun 2019 17:17:32: 6000000 INFO @ Sun, 02 Jun 2019 17:17:34: 7000000 INFO @ Sun, 02 Jun 2019 17:17:38: 7000000 INFO @ Sun, 02 Jun 2019 17:17:39: 6000000 INFO @ Sun, 02 Jun 2019 17:17:40: 8000000 INFO @ Sun, 02 Jun 2019 17:17:45: 8000000 INFO @ Sun, 02 Jun 2019 17:17:46: 9000000 INFO @ Sun, 02 Jun 2019 17:17:47: 7000000 INFO @ Sun, 02 Jun 2019 17:17:51: 9000000 INFO @ Sun, 02 Jun 2019 17:17:52: 10000000 INFO @ Sun, 02 Jun 2019 17:17:55: 8000000 INFO @ Sun, 02 Jun 2019 17:17:58: 10000000 INFO @ Sun, 02 Jun 2019 17:17:59: 11000000 INFO @ Sun, 02 Jun 2019 17:18:02: 9000000 INFO @ Sun, 02 Jun 2019 17:18:05: 12000000 INFO @ Sun, 02 Jun 2019 17:18:05: 11000000 INFO @ Sun, 02 Jun 2019 17:18:10: 10000000 INFO @ Sun, 02 Jun 2019 17:18:11: 13000000 INFO @ Sun, 02 Jun 2019 17:18:12: 12000000 INFO @ Sun, 02 Jun 2019 17:18:17: 11000000 INFO @ Sun, 02 Jun 2019 17:18:17: 14000000 INFO @ Sun, 02 Jun 2019 17:18:18: 13000000 INFO @ Sun, 02 Jun 2019 17:18:23: 15000000 INFO @ Sun, 02 Jun 2019 17:18:25: 14000000 INFO @ Sun, 02 Jun 2019 17:18:26: 12000000 INFO @ Sun, 02 Jun 2019 17:18:30: 16000000 INFO @ Sun, 02 Jun 2019 17:18:31: 15000000 INFO @ Sun, 02 Jun 2019 17:18:33: 13000000 INFO @ Sun, 02 Jun 2019 17:18:36: 17000000 INFO @ Sun, 02 Jun 2019 17:18:38: 16000000 INFO @ Sun, 02 Jun 2019 17:18:41: 14000000 INFO @ Sun, 02 Jun 2019 17:18:42: 18000000 INFO @ Sun, 02 Jun 2019 17:18:45: 17000000 INFO @ Sun, 02 Jun 2019 17:18:48: 19000000 INFO @ Sun, 02 Jun 2019 17:18:49: 15000000 INFO @ Sun, 02 Jun 2019 17:18:51: 18000000 INFO @ Sun, 02 Jun 2019 17:18:55: 20000000 INFO @ Sun, 02 Jun 2019 17:18:56: 16000000 INFO @ Sun, 02 Jun 2019 17:18:58: 19000000 INFO @ Sun, 02 Jun 2019 17:19:01: 21000000 INFO @ Sun, 02 Jun 2019 17:19:04: 17000000 INFO @ Sun, 02 Jun 2019 17:19:05: 20000000 INFO @ Sun, 02 Jun 2019 17:19:08: 22000000 INFO @ Sun, 02 Jun 2019 17:19:11: 21000000 INFO @ Sun, 02 Jun 2019 17:19:11: 18000000 INFO @ Sun, 02 Jun 2019 17:19:14: 23000000 INFO @ Sun, 02 Jun 2019 17:19:17: 22000000 INFO @ Sun, 02 Jun 2019 17:19:19: 19000000 INFO @ Sun, 02 Jun 2019 17:19:21: 24000000 INFO @ Sun, 02 Jun 2019 17:19:25: 23000000 INFO @ Sun, 02 Jun 2019 17:19:27: 20000000 INFO @ Sun, 02 Jun 2019 17:19:27: 25000000 INFO @ Sun, 02 Jun 2019 17:19:31: 24000000 INFO @ Sun, 02 Jun 2019 17:19:33: 26000000 INFO @ Sun, 02 Jun 2019 17:19:34: 21000000 INFO @ Sun, 02 Jun 2019 17:19:38: 25000000 INFO @ Sun, 02 Jun 2019 17:19:39: 27000000 INFO @ Sun, 02 Jun 2019 17:19:42: 22000000 INFO @ Sun, 02 Jun 2019 17:19:44: 26000000 INFO @ Sun, 02 Jun 2019 17:19:46: 28000000 INFO @ Sun, 02 Jun 2019 17:19:50: 23000000 INFO @ Sun, 02 Jun 2019 17:19:51: 27000000 INFO @ Sun, 02 Jun 2019 17:19:52: 29000000 INFO @ Sun, 02 Jun 2019 17:19:57: 28000000 INFO @ Sun, 02 Jun 2019 17:19:58: 24000000 INFO @ Sun, 02 Jun 2019 17:19:58: 30000000 INFO @ Sun, 02 Jun 2019 17:20:00: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:20:00: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:20:00: #1 total tags in treatment: 30156871 INFO @ Sun, 02 Jun 2019 17:20:00: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:20:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:20:00: #1 tags after filtering in treatment: 30156871 INFO @ Sun, 02 Jun 2019 17:20:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:20:00: #1 finished! INFO @ Sun, 02 Jun 2019 17:20:00: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:20:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:20:03: #2 number of paired peaks: 80 WARNING @ Sun, 02 Jun 2019 17:20:03: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:20:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:20:04: 29000000 INFO @ Sun, 02 Jun 2019 17:20:05: 25000000 INFO @ Sun, 02 Jun 2019 17:20:10: 30000000 INFO @ Sun, 02 Jun 2019 17:20:12: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:20:12: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:20:12: #1 total tags in treatment: 30156871 INFO @ Sun, 02 Jun 2019 17:20:12: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:20:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:20:12: #1 tags after filtering in treatment: 30156871 INFO @ Sun, 02 Jun 2019 17:20:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:20:12: #1 finished! INFO @ Sun, 02 Jun 2019 17:20:12: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:20:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:20:13: 26000000 INFO @ Sun, 02 Jun 2019 17:20:15: #2 number of paired peaks: 80 WARNING @ Sun, 02 Jun 2019 17:20:15: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:20:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:20:20: 27000000 INFO @ Sun, 02 Jun 2019 17:20:27: 28000000 INFO @ Sun, 02 Jun 2019 17:20:35: 29000000 INFO @ Sun, 02 Jun 2019 17:20:42: 30000000 INFO @ Sun, 02 Jun 2019 17:20:44: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 17:20:44: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 17:20:44: #1 total tags in treatment: 30156871 INFO @ Sun, 02 Jun 2019 17:20:44: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:20:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:20:44: #1 tags after filtering in treatment: 30156871 INFO @ Sun, 02 Jun 2019 17:20:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:20:44: #1 finished! INFO @ Sun, 02 Jun 2019 17:20:44: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:20:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:20:47: #2 number of paired peaks: 80 WARNING @ Sun, 02 Jun 2019 17:20:47: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:20:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX2737089/SRX2737089.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。