Job ID = 1291865 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,812,743 reads read : 18,812,743 reads written : 18,812,743 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:50 18812743 reads; of these: 18812743 (100.00%) were unpaired; of these: 150234 (0.80%) aligned 0 times 15590771 (82.87%) aligned exactly 1 time 3071738 (16.33%) aligned >1 times 99.20% overall alignment rate Time searching: 00:04:50 Overall time: 00:04:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8433557 / 18662509 = 0.4519 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:01:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:01:22: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:01:22: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:01:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:01:22: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:01:22: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:01:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:01:22: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:01:22: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:01:31: 1000000 INFO @ Sun, 02 Jun 2019 17:01:31: 1000000 INFO @ Sun, 02 Jun 2019 17:01:32: 1000000 INFO @ Sun, 02 Jun 2019 17:01:39: 2000000 INFO @ Sun, 02 Jun 2019 17:01:39: 2000000 INFO @ Sun, 02 Jun 2019 17:01:41: 2000000 INFO @ Sun, 02 Jun 2019 17:01:47: 3000000 INFO @ Sun, 02 Jun 2019 17:01:47: 3000000 INFO @ Sun, 02 Jun 2019 17:01:50: 3000000 INFO @ Sun, 02 Jun 2019 17:01:55: 4000000 INFO @ Sun, 02 Jun 2019 17:01:55: 4000000 INFO @ Sun, 02 Jun 2019 17:01:57: 4000000 INFO @ Sun, 02 Jun 2019 17:02:03: 5000000 INFO @ Sun, 02 Jun 2019 17:02:03: 5000000 INFO @ Sun, 02 Jun 2019 17:02:04: 5000000 INFO @ Sun, 02 Jun 2019 17:02:11: 6000000 INFO @ Sun, 02 Jun 2019 17:02:11: 6000000 INFO @ Sun, 02 Jun 2019 17:02:11: 6000000 INFO @ Sun, 02 Jun 2019 17:02:18: 7000000 INFO @ Sun, 02 Jun 2019 17:02:18: 7000000 INFO @ Sun, 02 Jun 2019 17:02:19: 7000000 INFO @ Sun, 02 Jun 2019 17:02:25: 8000000 INFO @ Sun, 02 Jun 2019 17:02:26: 8000000 INFO @ Sun, 02 Jun 2019 17:02:26: 8000000 INFO @ Sun, 02 Jun 2019 17:02:32: 9000000 INFO @ Sun, 02 Jun 2019 17:02:34: 9000000 INFO @ Sun, 02 Jun 2019 17:02:34: 9000000 INFO @ Sun, 02 Jun 2019 17:02:39: 10000000 INFO @ Sun, 02 Jun 2019 17:02:41: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:02:41: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:02:41: #1 total tags in treatment: 10228952 INFO @ Sun, 02 Jun 2019 17:02:41: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:02:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:02:41: #1 tags after filtering in treatment: 10228952 INFO @ Sun, 02 Jun 2019 17:02:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:02:41: #1 finished! INFO @ Sun, 02 Jun 2019 17:02:41: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:02:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:02:42: 10000000 INFO @ Sun, 02 Jun 2019 17:02:42: 10000000 INFO @ Sun, 02 Jun 2019 17:02:42: #2 number of paired peaks: 426 WARNING @ Sun, 02 Jun 2019 17:02:42: Fewer paired peaks (426) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 426 pairs to build model! INFO @ Sun, 02 Jun 2019 17:02:42: start model_add_line... INFO @ Sun, 02 Jun 2019 17:02:42: start X-correlation... INFO @ Sun, 02 Jun 2019 17:02:42: end of X-cor INFO @ Sun, 02 Jun 2019 17:02:42: #2 finished! INFO @ Sun, 02 Jun 2019 17:02:42: #2 predicted fragment length is 47 bps INFO @ Sun, 02 Jun 2019 17:02:42: #2 alternative fragment length(s) may be 2,47 bps INFO @ Sun, 02 Jun 2019 17:02:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.20_model.r WARNING @ Sun, 02 Jun 2019 17:02:42: #2 Since the d (47) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 17:02:42: #2 You may need to consider one of the other alternative d(s): 2,47 WARNING @ Sun, 02 Jun 2019 17:02:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 17:02:42: #3 Call peaks... INFO @ Sun, 02 Jun 2019 17:02:42: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 17:02:44: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:02:44: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:02:44: #1 total tags in treatment: 10228952 INFO @ Sun, 02 Jun 2019 17:02:44: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:02:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:02:44: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:02:44: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:02:44: #1 total tags in treatment: 10228952 INFO @ Sun, 02 Jun 2019 17:02:44: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:02:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:02:44: #1 tags after filtering in treatment: 10228952 INFO @ Sun, 02 Jun 2019 17:02:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:02:44: #1 finished! INFO @ Sun, 02 Jun 2019 17:02:44: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:02:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:02:44: #1 tags after filtering in treatment: 10228952 INFO @ Sun, 02 Jun 2019 17:02:44: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:02:44: #1 finished! INFO @ Sun, 02 Jun 2019 17:02:44: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:02:44: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:02:45: #2 number of paired peaks: 426 WARNING @ Sun, 02 Jun 2019 17:02:45: Fewer paired peaks (426) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 426 pairs to build model! INFO @ Sun, 02 Jun 2019 17:02:45: start model_add_line... INFO @ Sun, 02 Jun 2019 17:02:45: start X-correlation... INFO @ Sun, 02 Jun 2019 17:02:45: #2 number of paired peaks: 426 WARNING @ Sun, 02 Jun 2019 17:02:45: Fewer paired peaks (426) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 426 pairs to build model! INFO @ Sun, 02 Jun 2019 17:02:45: start model_add_line... INFO @ Sun, 02 Jun 2019 17:02:45: end of X-cor INFO @ Sun, 02 Jun 2019 17:02:45: #2 finished! INFO @ Sun, 02 Jun 2019 17:02:45: #2 predicted fragment length is 47 bps INFO @ Sun, 02 Jun 2019 17:02:45: #2 alternative fragment length(s) may be 2,47 bps INFO @ Sun, 02 Jun 2019 17:02:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.05_model.r WARNING @ Sun, 02 Jun 2019 17:02:45: #2 Since the d (47) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 17:02:45: #2 You may need to consider one of the other alternative d(s): 2,47 WARNING @ Sun, 02 Jun 2019 17:02:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 17:02:45: #3 Call peaks... INFO @ Sun, 02 Jun 2019 17:02:45: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 17:02:45: start X-correlation... INFO @ Sun, 02 Jun 2019 17:02:45: end of X-cor INFO @ Sun, 02 Jun 2019 17:02:45: #2 finished! INFO @ Sun, 02 Jun 2019 17:02:45: #2 predicted fragment length is 47 bps INFO @ Sun, 02 Jun 2019 17:02:45: #2 alternative fragment length(s) may be 2,47 bps INFO @ Sun, 02 Jun 2019 17:02:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.10_model.r WARNING @ Sun, 02 Jun 2019 17:02:45: #2 Since the d (47) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 17:02:45: #2 You may need to consider one of the other alternative d(s): 2,47 WARNING @ Sun, 02 Jun 2019 17:02:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 17:02:45: #3 Call peaks... INFO @ Sun, 02 Jun 2019 17:02:45: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 17:03:10: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 17:03:12: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 17:03:12: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 17:03:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.20_peaks.xls INFO @ Sun, 02 Jun 2019 17:03:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 17:03:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.20_summits.bed INFO @ Sun, 02 Jun 2019 17:03:23: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (220 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:03:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.05_peaks.xls INFO @ Sun, 02 Jun 2019 17:03:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 17:03:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.05_summits.bed INFO @ Sun, 02 Jun 2019 17:03:25: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (760 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:03:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.10_peaks.xls INFO @ Sun, 02 Jun 2019 17:03:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 17:03:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX257700/SRX257700.10_summits.bed INFO @ Sun, 02 Jun 2019 17:03:26: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (553 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。