Job ID = 1291777 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-02T07:25:21 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T07:25:21 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766599' 2019-06-02T07:25:30 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766599' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-02T07:25:35 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T07:25:35 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766599' 2019-06-02T07:25:35 fasterq-dump.2.9.6 err: invalid accession 'SRR766599' spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T07:40:36 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T07:40:36 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766604' 2019-06-02T07:40:46 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766604' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-02T07:40:46 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T08:00:22 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T08:00:22 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766610' 2019-06-02T08:00:32 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766610' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T08:30:37 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T08:30:37 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766620' 2019-06-02T08:30:46 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766620' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-02T08:30:46 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T09:05:36 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T09:05:36 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766631' 2019-06-02T09:05:45 fasterq-dump.2.9.6 err: cmn_iter.c cmn_get_acc_type( 'SRR766631', 'NAME' ).VDBManagerOpenTableRead() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T09:30:31 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T09:30:31 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766639' 2019-06-02T09:30:41 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766639' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-02T09:30:41 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T09:35:52 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T09:35:52 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766640' 2019-06-02T09:36:02 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766640' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 789,812 reads read : 1,579,624 reads written : 1,579,624 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 2019-06-02T09:50:33 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-02T09:50:33 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra8/SRR/000748/SRR766645' 2019-06-02T09:50:42 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR766645' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-02T09:50:42 fasterq-dump.2.9.6 err: make_fastq_iter() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 4,000,000 reads read : 8,000,000 reads written : 8,000,000 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:38:32 184789812 reads; of these: 184789812 (100.00%) were paired; of these: 167448873 (90.62%) aligned concordantly 0 times 14449514 (7.82%) aligned concordantly exactly 1 time 2891425 (1.56%) aligned concordantly >1 times ---- 167448873 pairs aligned concordantly 0 times; of these: 89498 (0.05%) aligned discordantly 1 time ---- 167359375 pairs aligned 0 times concordantly or discordantly; of these: 334718750 mates make up the pairs; of these: 333203372 (99.55%) aligned 0 times 833768 (0.25%) aligned exactly 1 time 681610 (0.20%) aligned >1 times 9.84% overall alignment rate Time searching: 00:38:33 Overall time: 00:38:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 9903953 / 17356252 = 0.5706 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 19:43:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:43:35: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:43:35: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:43:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:43:35: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:43:35: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:43:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 19:43:35: #1 read tag files... INFO @ Sun, 02 Jun 2019 19:43:35: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 19:43:44: 1000000 INFO @ Sun, 02 Jun 2019 19:43:44: 1000000 INFO @ Sun, 02 Jun 2019 19:43:44: 1000000 INFO @ Sun, 02 Jun 2019 19:43:52: 2000000 INFO @ Sun, 02 Jun 2019 19:43:52: 2000000 INFO @ Sun, 02 Jun 2019 19:43:53: 2000000 INFO @ Sun, 02 Jun 2019 19:43:59: 3000000 INFO @ Sun, 02 Jun 2019 19:43:59: 3000000 INFO @ Sun, 02 Jun 2019 19:44:01: 3000000 INFO @ Sun, 02 Jun 2019 19:44:07: 4000000 INFO @ Sun, 02 Jun 2019 19:44:07: 4000000 INFO @ Sun, 02 Jun 2019 19:44:09: 4000000 INFO @ Sun, 02 Jun 2019 19:44:16: 5000000 INFO @ Sun, 02 Jun 2019 19:44:16: 5000000 INFO @ Sun, 02 Jun 2019 19:44:17: 5000000 INFO @ Sun, 02 Jun 2019 19:44:25: 6000000 INFO @ Sun, 02 Jun 2019 19:44:25: 6000000 INFO @ Sun, 02 Jun 2019 19:44:25: 6000000 INFO @ Sun, 02 Jun 2019 19:44:33: 7000000 INFO @ Sun, 02 Jun 2019 19:44:33: 7000000 INFO @ Sun, 02 Jun 2019 19:44:33: 7000000 INFO @ Sun, 02 Jun 2019 19:44:41: 8000000 INFO @ Sun, 02 Jun 2019 19:44:41: 8000000 INFO @ Sun, 02 Jun 2019 19:44:42: 8000000 INFO @ Sun, 02 Jun 2019 19:44:49: 9000000 INFO @ Sun, 02 Jun 2019 19:44:49: 9000000 INFO @ Sun, 02 Jun 2019 19:44:50: 9000000 INFO @ Sun, 02 Jun 2019 19:44:57: 10000000 INFO @ Sun, 02 Jun 2019 19:44:57: 10000000 INFO @ Sun, 02 Jun 2019 19:44:58: 10000000 INFO @ Sun, 02 Jun 2019 19:45:05: 11000000 INFO @ Sun, 02 Jun 2019 19:45:05: 11000000 INFO @ Sun, 02 Jun 2019 19:45:06: 11000000 INFO @ Sun, 02 Jun 2019 19:45:13: 12000000 INFO @ Sun, 02 Jun 2019 19:45:13: 12000000 INFO @ Sun, 02 Jun 2019 19:45:15: 12000000 INFO @ Sun, 02 Jun 2019 19:45:21: 13000000 INFO @ Sun, 02 Jun 2019 19:45:21: 13000000 INFO @ Sun, 02 Jun 2019 19:45:23: 13000000 INFO @ Sun, 02 Jun 2019 19:45:29: 14000000 INFO @ Sun, 02 Jun 2019 19:45:29: 14000000 INFO @ Sun, 02 Jun 2019 19:45:31: 14000000 INFO @ Sun, 02 Jun 2019 19:45:37: 15000000 INFO @ Sun, 02 Jun 2019 19:45:37: 15000000 INFO @ Sun, 02 Jun 2019 19:45:39: 15000000 INFO @ Sun, 02 Jun 2019 19:45:45: 16000000 INFO @ Sun, 02 Jun 2019 19:45:45: 16000000 INFO @ Sun, 02 Jun 2019 19:45:47: 16000000 INFO @ Sun, 02 Jun 2019 19:45:49: #1 tag size is determined as 25 bps INFO @ Sun, 02 Jun 2019 19:45:49: #1 tag size = 25 INFO @ Sun, 02 Jun 2019 19:45:49: #1 total tags in treatment: 7498503 INFO @ Sun, 02 Jun 2019 19:45:49: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:45:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:45:50: #1 tags after filtering in treatment: 6424229 INFO @ Sun, 02 Jun 2019 19:45:50: #1 Redundant rate of treatment: 0.14 INFO @ Sun, 02 Jun 2019 19:45:50: #1 finished! INFO @ Sun, 02 Jun 2019 19:45:50: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:45:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:45:50: #1 tag size is determined as 25 bps INFO @ Sun, 02 Jun 2019 19:45:50: #1 tag size = 25 INFO @ Sun, 02 Jun 2019 19:45:50: #1 total tags in treatment: 7498503 INFO @ Sun, 02 Jun 2019 19:45:50: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:45:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:45:50: #1 tags after filtering in treatment: 6424229 INFO @ Sun, 02 Jun 2019 19:45:50: #1 Redundant rate of treatment: 0.14 INFO @ Sun, 02 Jun 2019 19:45:50: #1 finished! INFO @ Sun, 02 Jun 2019 19:45:50: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:45:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:45:50: #2 number of paired peaks: 536 WARNING @ Sun, 02 Jun 2019 19:45:50: Fewer paired peaks (536) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 536 pairs to build model! INFO @ Sun, 02 Jun 2019 19:45:50: start model_add_line... INFO @ Sun, 02 Jun 2019 19:45:50: start X-correlation... INFO @ Sun, 02 Jun 2019 19:45:50: end of X-cor INFO @ Sun, 02 Jun 2019 19:45:50: #2 finished! INFO @ Sun, 02 Jun 2019 19:45:50: #2 predicted fragment length is 117 bps INFO @ Sun, 02 Jun 2019 19:45:50: #2 alternative fragment length(s) may be 3,117,139 bps INFO @ Sun, 02 Jun 2019 19:45:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.20_model.r INFO @ Sun, 02 Jun 2019 19:45:50: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:45:50: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:45:50: #2 number of paired peaks: 536 WARNING @ Sun, 02 Jun 2019 19:45:50: Fewer paired peaks (536) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 536 pairs to build model! INFO @ Sun, 02 Jun 2019 19:45:50: start model_add_line... INFO @ Sun, 02 Jun 2019 19:45:51: start X-correlation... INFO @ Sun, 02 Jun 2019 19:45:51: end of X-cor INFO @ Sun, 02 Jun 2019 19:45:51: #2 finished! INFO @ Sun, 02 Jun 2019 19:45:51: #2 predicted fragment length is 117 bps INFO @ Sun, 02 Jun 2019 19:45:51: #2 alternative fragment length(s) may be 3,117,139 bps INFO @ Sun, 02 Jun 2019 19:45:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.10_model.r INFO @ Sun, 02 Jun 2019 19:45:51: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:45:51: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:45:51: #1 tag size is determined as 25 bps INFO @ Sun, 02 Jun 2019 19:45:51: #1 tag size = 25 INFO @ Sun, 02 Jun 2019 19:45:51: #1 total tags in treatment: 7498503 INFO @ Sun, 02 Jun 2019 19:45:51: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 19:45:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 19:45:52: #1 tags after filtering in treatment: 6424229 INFO @ Sun, 02 Jun 2019 19:45:52: #1 Redundant rate of treatment: 0.14 INFO @ Sun, 02 Jun 2019 19:45:52: #1 finished! INFO @ Sun, 02 Jun 2019 19:45:52: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 19:45:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 19:45:52: #2 number of paired peaks: 536 WARNING @ Sun, 02 Jun 2019 19:45:52: Fewer paired peaks (536) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 536 pairs to build model! INFO @ Sun, 02 Jun 2019 19:45:52: start model_add_line... INFO @ Sun, 02 Jun 2019 19:45:52: start X-correlation... INFO @ Sun, 02 Jun 2019 19:45:52: end of X-cor INFO @ Sun, 02 Jun 2019 19:45:52: #2 finished! INFO @ Sun, 02 Jun 2019 19:45:52: #2 predicted fragment length is 117 bps INFO @ Sun, 02 Jun 2019 19:45:52: #2 alternative fragment length(s) may be 3,117,139 bps INFO @ Sun, 02 Jun 2019 19:45:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.05_model.r INFO @ Sun, 02 Jun 2019 19:45:52: #3 Call peaks... INFO @ Sun, 02 Jun 2019 19:45:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 19:46:11: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:46:11: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:46:12: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 19:46:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.20_peaks.xls INFO @ Sun, 02 Jun 2019 19:46:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:46:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.20_summits.bed INFO @ Sun, 02 Jun 2019 19:46:21: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (115 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:46:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.10_peaks.xls INFO @ Sun, 02 Jun 2019 19:46:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:46:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.10_summits.bed INFO @ Sun, 02 Jun 2019 19:46:21: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (231 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 19:46:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.05_peaks.xls INFO @ Sun, 02 Jun 2019 19:46:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 19:46:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX246038/SRX246038.05_summits.bed INFO @ Sun, 02 Jun 2019 19:46:22: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (386 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。