Job ID = 1291682 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 35,077,847 reads read : 35,077,847 reads written : 35,077,847 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:59 35077847 reads; of these: 35077847 (100.00%) were unpaired; of these: 2382807 (6.79%) aligned 0 times 28159803 (80.28%) aligned exactly 1 time 4535237 (12.93%) aligned >1 times 93.21% overall alignment rate Time searching: 00:07:59 Overall time: 00:07:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5393455 / 32695040 = 0.1650 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 16:34:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:34:14: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:34:14: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:34:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:34:14: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:34:14: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:34:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:34:14: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:34:14: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:34:23: 1000000 INFO @ Sun, 02 Jun 2019 16:34:25: 1000000 INFO @ Sun, 02 Jun 2019 16:34:26: 1000000 INFO @ Sun, 02 Jun 2019 16:34:32: 2000000 INFO @ Sun, 02 Jun 2019 16:34:36: 2000000 INFO @ Sun, 02 Jun 2019 16:34:37: 2000000 INFO @ Sun, 02 Jun 2019 16:34:41: 3000000 INFO @ Sun, 02 Jun 2019 16:34:47: 3000000 INFO @ Sun, 02 Jun 2019 16:34:48: 3000000 INFO @ Sun, 02 Jun 2019 16:34:50: 4000000 INFO @ Sun, 02 Jun 2019 16:34:58: 4000000 INFO @ Sun, 02 Jun 2019 16:34:58: 5000000 INFO @ Sun, 02 Jun 2019 16:34:59: 4000000 INFO @ Sun, 02 Jun 2019 16:35:07: 6000000 INFO @ Sun, 02 Jun 2019 16:35:09: 5000000 INFO @ Sun, 02 Jun 2019 16:35:10: 5000000 INFO @ Sun, 02 Jun 2019 16:35:17: 7000000 INFO @ Sun, 02 Jun 2019 16:35:20: 6000000 INFO @ Sun, 02 Jun 2019 16:35:21: 6000000 INFO @ Sun, 02 Jun 2019 16:35:26: 8000000 INFO @ Sun, 02 Jun 2019 16:35:31: 7000000 INFO @ Sun, 02 Jun 2019 16:35:32: 7000000 INFO @ Sun, 02 Jun 2019 16:35:35: 9000000 INFO @ Sun, 02 Jun 2019 16:35:42: 8000000 INFO @ Sun, 02 Jun 2019 16:35:44: 8000000 INFO @ Sun, 02 Jun 2019 16:35:44: 10000000 INFO @ Sun, 02 Jun 2019 16:35:52: 9000000 INFO @ Sun, 02 Jun 2019 16:35:53: 11000000 INFO @ Sun, 02 Jun 2019 16:35:55: 9000000 INFO @ Sun, 02 Jun 2019 16:36:01: 12000000 INFO @ Sun, 02 Jun 2019 16:36:02: 10000000 INFO @ Sun, 02 Jun 2019 16:36:06: 10000000 INFO @ Sun, 02 Jun 2019 16:36:10: 13000000 INFO @ Sun, 02 Jun 2019 16:36:13: 11000000 INFO @ Sun, 02 Jun 2019 16:36:17: 11000000 INFO @ Sun, 02 Jun 2019 16:36:18: 14000000 INFO @ Sun, 02 Jun 2019 16:36:23: 12000000 INFO @ Sun, 02 Jun 2019 16:36:27: 15000000 INFO @ Sun, 02 Jun 2019 16:36:28: 12000000 INFO @ Sun, 02 Jun 2019 16:36:33: 13000000 INFO @ Sun, 02 Jun 2019 16:36:35: 16000000 INFO @ Sun, 02 Jun 2019 16:36:39: 13000000 INFO @ Sun, 02 Jun 2019 16:36:43: 14000000 INFO @ Sun, 02 Jun 2019 16:36:44: 17000000 INFO @ Sun, 02 Jun 2019 16:36:50: 14000000 INFO @ Sun, 02 Jun 2019 16:36:53: 18000000 INFO @ Sun, 02 Jun 2019 16:36:54: 15000000 INFO @ Sun, 02 Jun 2019 16:37:00: 15000000 INFO @ Sun, 02 Jun 2019 16:37:01: 19000000 INFO @ Sun, 02 Jun 2019 16:37:04: 16000000 INFO @ Sun, 02 Jun 2019 16:37:10: 20000000 INFO @ Sun, 02 Jun 2019 16:37:11: 16000000 INFO @ Sun, 02 Jun 2019 16:37:14: 17000000 INFO @ Sun, 02 Jun 2019 16:37:18: 21000000 INFO @ Sun, 02 Jun 2019 16:37:21: 17000000 INFO @ Sun, 02 Jun 2019 16:37:24: 18000000 INFO @ Sun, 02 Jun 2019 16:37:27: 22000000 INFO @ Sun, 02 Jun 2019 16:37:31: 18000000 INFO @ Sun, 02 Jun 2019 16:37:34: 19000000 INFO @ Sun, 02 Jun 2019 16:37:35: 23000000 INFO @ Sun, 02 Jun 2019 16:37:41: 19000000 INFO @ Sun, 02 Jun 2019 16:37:44: 24000000 INFO @ Sun, 02 Jun 2019 16:37:45: 20000000 INFO @ Sun, 02 Jun 2019 16:37:52: 20000000 INFO @ Sun, 02 Jun 2019 16:37:52: 25000000 INFO @ Sun, 02 Jun 2019 16:37:55: 21000000 INFO @ Sun, 02 Jun 2019 16:38:01: 26000000 INFO @ Sun, 02 Jun 2019 16:38:02: 21000000 INFO @ Sun, 02 Jun 2019 16:38:05: 22000000 INFO @ Sun, 02 Jun 2019 16:38:09: 27000000 INFO @ Sun, 02 Jun 2019 16:38:12: 22000000 INFO @ Sun, 02 Jun 2019 16:38:12: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 16:38:12: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 16:38:12: #1 total tags in treatment: 27301585 INFO @ Sun, 02 Jun 2019 16:38:12: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:38:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:38:13: #1 tags after filtering in treatment: 27301585 INFO @ Sun, 02 Jun 2019 16:38:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:38:13: #1 finished! INFO @ Sun, 02 Jun 2019 16:38:13: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:38:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:38:15: #2 number of paired peaks: 92 WARNING @ Sun, 02 Jun 2019 16:38:15: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:38:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 16:38:15: 23000000 INFO @ Sun, 02 Jun 2019 16:38:22: 23000000 INFO @ Sun, 02 Jun 2019 16:38:25: 24000000 INFO @ Sun, 02 Jun 2019 16:38:32: 24000000 INFO @ Sun, 02 Jun 2019 16:38:35: 25000000 INFO @ Sun, 02 Jun 2019 16:38:42: 25000000 INFO @ Sun, 02 Jun 2019 16:38:45: 26000000 INFO @ Sun, 02 Jun 2019 16:38:52: 26000000 INFO @ Sun, 02 Jun 2019 16:38:55: 27000000 INFO @ Sun, 02 Jun 2019 16:38:58: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 16:38:58: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 16:38:58: #1 total tags in treatment: 27301585 INFO @ Sun, 02 Jun 2019 16:38:58: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:38:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:38:59: #1 tags after filtering in treatment: 27301585 INFO @ Sun, 02 Jun 2019 16:38:59: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:38:59: #1 finished! INFO @ Sun, 02 Jun 2019 16:38:59: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:38:59: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:39:01: #2 number of paired peaks: 92 WARNING @ Sun, 02 Jun 2019 16:39:01: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:39:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 16:39:01: 27000000 INFO @ Sun, 02 Jun 2019 16:39:04: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 16:39:04: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 16:39:04: #1 total tags in treatment: 27301585 INFO @ Sun, 02 Jun 2019 16:39:04: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:39:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:39:05: #1 tags after filtering in treatment: 27301585 INFO @ Sun, 02 Jun 2019 16:39:05: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:39:05: #1 finished! INFO @ Sun, 02 Jun 2019 16:39:05: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:39:05: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:39:07: #2 number of paired peaks: 92 WARNING @ Sun, 02 Jun 2019 16:39:07: Too few paired peaks (92) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:39:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX216728/SRX216728.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。