Job ID = 1291670 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 46,198,117 reads read : 46,198,117 reads written : 46,198,117 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:49 46198117 reads; of these: 46198117 (100.00%) were unpaired; of these: 4247745 (9.19%) aligned 0 times 36169664 (78.29%) aligned exactly 1 time 5780708 (12.51%) aligned >1 times 90.81% overall alignment rate Time searching: 00:10:49 Overall time: 00:10:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 10990691 / 41950372 = 0.2620 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 16:37:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:37:37: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:37:37: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:37:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:37:37: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:37:37: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:37:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:37:37: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:37:37: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:37:45: 1000000 INFO @ Sun, 02 Jun 2019 16:37:45: 1000000 INFO @ Sun, 02 Jun 2019 16:37:46: 1000000 INFO @ Sun, 02 Jun 2019 16:37:53: 2000000 INFO @ Sun, 02 Jun 2019 16:37:53: 2000000 INFO @ Sun, 02 Jun 2019 16:37:55: 2000000 INFO @ Sun, 02 Jun 2019 16:38:01: 3000000 INFO @ Sun, 02 Jun 2019 16:38:01: 3000000 INFO @ Sun, 02 Jun 2019 16:38:04: 3000000 INFO @ Sun, 02 Jun 2019 16:38:09: 4000000 INFO @ Sun, 02 Jun 2019 16:38:09: 4000000 INFO @ Sun, 02 Jun 2019 16:38:13: 4000000 INFO @ Sun, 02 Jun 2019 16:38:17: 5000000 INFO @ Sun, 02 Jun 2019 16:38:17: 5000000 INFO @ Sun, 02 Jun 2019 16:38:22: 5000000 INFO @ Sun, 02 Jun 2019 16:38:25: 6000000 INFO @ Sun, 02 Jun 2019 16:38:25: 6000000 INFO @ Sun, 02 Jun 2019 16:38:30: 6000000 INFO @ Sun, 02 Jun 2019 16:38:32: 7000000 INFO @ Sun, 02 Jun 2019 16:38:33: 7000000 INFO @ Sun, 02 Jun 2019 16:38:39: 7000000 INFO @ Sun, 02 Jun 2019 16:38:40: 8000000 INFO @ Sun, 02 Jun 2019 16:38:40: 8000000 INFO @ Sun, 02 Jun 2019 16:38:47: 9000000 INFO @ Sun, 02 Jun 2019 16:38:48: 8000000 INFO @ Sun, 02 Jun 2019 16:38:48: 9000000 INFO @ Sun, 02 Jun 2019 16:38:55: 10000000 INFO @ Sun, 02 Jun 2019 16:38:55: 10000000 INFO @ Sun, 02 Jun 2019 16:38:56: 9000000 INFO @ Sun, 02 Jun 2019 16:39:02: 11000000 INFO @ Sun, 02 Jun 2019 16:39:03: 11000000 INFO @ Sun, 02 Jun 2019 16:39:05: 10000000 INFO @ Sun, 02 Jun 2019 16:39:09: 12000000 INFO @ Sun, 02 Jun 2019 16:39:10: 12000000 INFO @ Sun, 02 Jun 2019 16:39:13: 11000000 INFO @ Sun, 02 Jun 2019 16:39:16: 13000000 INFO @ Sun, 02 Jun 2019 16:39:18: 13000000 INFO @ Sun, 02 Jun 2019 16:39:22: 12000000 INFO @ Sun, 02 Jun 2019 16:39:24: 14000000 INFO @ Sun, 02 Jun 2019 16:39:25: 14000000 INFO @ Sun, 02 Jun 2019 16:39:30: 13000000 INFO @ Sun, 02 Jun 2019 16:39:31: 15000000 INFO @ Sun, 02 Jun 2019 16:39:33: 15000000 INFO @ Sun, 02 Jun 2019 16:39:38: 16000000 INFO @ Sun, 02 Jun 2019 16:39:39: 14000000 INFO @ Sun, 02 Jun 2019 16:39:40: 16000000 INFO @ Sun, 02 Jun 2019 16:39:46: 17000000 INFO @ Sun, 02 Jun 2019 16:39:47: 15000000 INFO @ Sun, 02 Jun 2019 16:39:47: 17000000 INFO @ Sun, 02 Jun 2019 16:39:53: 18000000 INFO @ Sun, 02 Jun 2019 16:39:55: 18000000 INFO @ Sun, 02 Jun 2019 16:39:55: 16000000 INFO @ Sun, 02 Jun 2019 16:40:00: 19000000 INFO @ Sun, 02 Jun 2019 16:40:02: 19000000 INFO @ Sun, 02 Jun 2019 16:40:04: 17000000 INFO @ Sun, 02 Jun 2019 16:40:08: 20000000 INFO @ Sun, 02 Jun 2019 16:40:09: 20000000 INFO @ Sun, 02 Jun 2019 16:40:12: 18000000 INFO @ Sun, 02 Jun 2019 16:40:15: 21000000 INFO @ Sun, 02 Jun 2019 16:40:16: 21000000 INFO @ Sun, 02 Jun 2019 16:40:20: 19000000 INFO @ Sun, 02 Jun 2019 16:40:22: 22000000 INFO @ Sun, 02 Jun 2019 16:40:24: 22000000 INFO @ Sun, 02 Jun 2019 16:40:29: 20000000 INFO @ Sun, 02 Jun 2019 16:40:29: 23000000 INFO @ Sun, 02 Jun 2019 16:40:31: 23000000 INFO @ Sun, 02 Jun 2019 16:40:37: 24000000 INFO @ Sun, 02 Jun 2019 16:40:37: 21000000 INFO @ Sun, 02 Jun 2019 16:40:38: 24000000 INFO @ Sun, 02 Jun 2019 16:40:44: 25000000 INFO @ Sun, 02 Jun 2019 16:40:46: 22000000 INFO @ Sun, 02 Jun 2019 16:40:46: 25000000 INFO @ Sun, 02 Jun 2019 16:40:51: 26000000 INFO @ Sun, 02 Jun 2019 16:40:53: 26000000 INFO @ Sun, 02 Jun 2019 16:40:54: 23000000 INFO @ Sun, 02 Jun 2019 16:40:59: 27000000 INFO @ Sun, 02 Jun 2019 16:41:00: 27000000 INFO @ Sun, 02 Jun 2019 16:41:02: 24000000 INFO @ Sun, 02 Jun 2019 16:41:06: 28000000 INFO @ Sun, 02 Jun 2019 16:41:08: 28000000 INFO @ Sun, 02 Jun 2019 16:41:11: 25000000 INFO @ Sun, 02 Jun 2019 16:41:13: 29000000 INFO @ Sun, 02 Jun 2019 16:41:15: 29000000 INFO @ Sun, 02 Jun 2019 16:41:19: 26000000 INFO @ Sun, 02 Jun 2019 16:41:20: 30000000 INFO @ Sun, 02 Jun 2019 16:41:22: 30000000 INFO @ Sun, 02 Jun 2019 16:41:27: 27000000 INFO @ Sun, 02 Jun 2019 16:41:28: #1 tag size is determined as 49 bps INFO @ Sun, 02 Jun 2019 16:41:28: #1 tag size = 49 INFO @ Sun, 02 Jun 2019 16:41:28: #1 total tags in treatment: 30959681 INFO @ Sun, 02 Jun 2019 16:41:28: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:41:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:41:28: #1 tags after filtering in treatment: 30959681 INFO @ Sun, 02 Jun 2019 16:41:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:41:28: #1 finished! INFO @ Sun, 02 Jun 2019 16:41:28: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:41:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:41:29: #1 tag size is determined as 49 bps INFO @ Sun, 02 Jun 2019 16:41:29: #1 tag size = 49 INFO @ Sun, 02 Jun 2019 16:41:29: #1 total tags in treatment: 30959681 INFO @ Sun, 02 Jun 2019 16:41:29: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:41:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:41:30: #1 tags after filtering in treatment: 30959681 INFO @ Sun, 02 Jun 2019 16:41:30: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:41:30: #1 finished! INFO @ Sun, 02 Jun 2019 16:41:30: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:41:30: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:41:31: #2 number of paired peaks: 43 WARNING @ Sun, 02 Jun 2019 16:41:31: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:41:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 16:41:32: #2 number of paired peaks: 43 WARNING @ Sun, 02 Jun 2019 16:41:32: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:41:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 16:41:35: 28000000 INFO @ Sun, 02 Jun 2019 16:41:43: 29000000 INFO @ Sun, 02 Jun 2019 16:41:52: 30000000 INFO @ Sun, 02 Jun 2019 16:42:00: #1 tag size is determined as 49 bps INFO @ Sun, 02 Jun 2019 16:42:00: #1 tag size = 49 INFO @ Sun, 02 Jun 2019 16:42:00: #1 total tags in treatment: 30959681 INFO @ Sun, 02 Jun 2019 16:42:00: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:42:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:42:00: #1 tags after filtering in treatment: 30959681 INFO @ Sun, 02 Jun 2019 16:42:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:42:00: #1 finished! INFO @ Sun, 02 Jun 2019 16:42:00: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:42:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:42:03: #2 number of paired peaks: 43 WARNING @ Sun, 02 Jun 2019 16:42:03: Too few paired peaks (43) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:42:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1769998/SRX1769998.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。