Job ID = 2589395 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,775,877 reads read : 4,775,877 reads written : 4,775,877 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR494574.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:51 4775877 reads; of these: 4775877 (100.00%) were unpaired; of these: 107541 (2.25%) aligned 0 times 3779154 (79.13%) aligned exactly 1 time 889182 (18.62%) aligned >1 times 97.75% overall alignment rate Time searching: 00:00:51 Overall time: 00:00:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 353396 / 4668336 = 0.0757 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:35:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:35:56: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:35:56: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:35:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:35:57: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:35:57: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:35:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:35:58: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:35:58: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:36:03: 1000000 INFO @ Mon, 12 Aug 2019 17:36:03: 1000000 INFO @ Mon, 12 Aug 2019 17:36:07: 1000000 INFO @ Mon, 12 Aug 2019 17:36:10: 2000000 INFO @ Mon, 12 Aug 2019 17:36:10: 2000000 INFO @ Mon, 12 Aug 2019 17:36:15: 2000000 INFO @ Mon, 12 Aug 2019 17:36:17: 3000000 INFO @ Mon, 12 Aug 2019 17:36:18: 3000000 INFO @ Mon, 12 Aug 2019 17:36:23: 4000000 INFO @ Mon, 12 Aug 2019 17:36:25: 3000000 INFO @ Mon, 12 Aug 2019 17:36:25: 4000000 INFO @ Mon, 12 Aug 2019 17:36:25: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:36:25: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:36:25: #1 total tags in treatment: 4314940 INFO @ Mon, 12 Aug 2019 17:36:25: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:36:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:36:25: #1 tags after filtering in treatment: 4314940 INFO @ Mon, 12 Aug 2019 17:36:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:36:25: #1 finished! INFO @ Mon, 12 Aug 2019 17:36:25: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:36:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:36:26: #2 number of paired peaks: 477 WARNING @ Mon, 12 Aug 2019 17:36:26: Fewer paired peaks (477) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 477 pairs to build model! INFO @ Mon, 12 Aug 2019 17:36:26: start model_add_line... INFO @ Mon, 12 Aug 2019 17:36:26: start X-correlation... INFO @ Mon, 12 Aug 2019 17:36:26: end of X-cor INFO @ Mon, 12 Aug 2019 17:36:26: #2 finished! INFO @ Mon, 12 Aug 2019 17:36:26: #2 predicted fragment length is 32 bps INFO @ Mon, 12 Aug 2019 17:36:26: #2 alternative fragment length(s) may be 3,32,509,590,597 bps INFO @ Mon, 12 Aug 2019 17:36:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.10_model.r WARNING @ Mon, 12 Aug 2019 17:36:26: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:36:26: #2 You may need to consider one of the other alternative d(s): 3,32,509,590,597 WARNING @ Mon, 12 Aug 2019 17:36:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:36:26: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:36:26: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:36:27: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:36:27: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:36:27: #1 total tags in treatment: 4314940 INFO @ Mon, 12 Aug 2019 17:36:27: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:36:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:36:27: #1 tags after filtering in treatment: 4314940 INFO @ Mon, 12 Aug 2019 17:36:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:36:27: #1 finished! INFO @ Mon, 12 Aug 2019 17:36:27: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:36:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:36:28: #2 number of paired peaks: 477 WARNING @ Mon, 12 Aug 2019 17:36:28: Fewer paired peaks (477) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 477 pairs to build model! INFO @ Mon, 12 Aug 2019 17:36:28: start model_add_line... INFO @ Mon, 12 Aug 2019 17:36:28: start X-correlation... INFO @ Mon, 12 Aug 2019 17:36:28: end of X-cor INFO @ Mon, 12 Aug 2019 17:36:28: #2 finished! INFO @ Mon, 12 Aug 2019 17:36:28: #2 predicted fragment length is 32 bps INFO @ Mon, 12 Aug 2019 17:36:28: #2 alternative fragment length(s) may be 3,32,509,590,597 bps INFO @ Mon, 12 Aug 2019 17:36:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.05_model.r WARNING @ Mon, 12 Aug 2019 17:36:28: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:36:28: #2 You may need to consider one of the other alternative d(s): 3,32,509,590,597 WARNING @ Mon, 12 Aug 2019 17:36:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:36:28: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:36:28: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:36:34: 4000000 INFO @ Mon, 12 Aug 2019 17:36:36: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:36:36: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:36:36: #1 total tags in treatment: 4314940 INFO @ Mon, 12 Aug 2019 17:36:36: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:36:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:36:37: #1 tags after filtering in treatment: 4314940 INFO @ Mon, 12 Aug 2019 17:36:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:36:37: #1 finished! INFO @ Mon, 12 Aug 2019 17:36:37: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:36:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:36:37: #2 number of paired peaks: 477 WARNING @ Mon, 12 Aug 2019 17:36:37: Fewer paired peaks (477) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 477 pairs to build model! INFO @ Mon, 12 Aug 2019 17:36:37: start model_add_line... INFO @ Mon, 12 Aug 2019 17:36:37: start X-correlation... INFO @ Mon, 12 Aug 2019 17:36:37: end of X-cor INFO @ Mon, 12 Aug 2019 17:36:37: #2 finished! INFO @ Mon, 12 Aug 2019 17:36:37: #2 predicted fragment length is 32 bps INFO @ Mon, 12 Aug 2019 17:36:37: #2 alternative fragment length(s) may be 3,32,509,590,597 bps INFO @ Mon, 12 Aug 2019 17:36:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.20_model.r WARNING @ Mon, 12 Aug 2019 17:36:37: #2 Since the d (32) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:36:37: #2 You may need to consider one of the other alternative d(s): 3,32,509,590,597 WARNING @ Mon, 12 Aug 2019 17:36:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:36:37: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:36:37: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:36:38: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:36:40: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:36:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:36:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:36:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.10_summits.bed INFO @ Mon, 12 Aug 2019 17:36:44: Done! pass1 - making usageList (6 chroms): 3 millis pass2 - checking and writing primary data (275 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:36:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:36:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:36:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.05_summits.bed INFO @ Mon, 12 Aug 2019 17:36:46: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (573 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:36:50: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:36:56: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:36:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:36:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146413/SRX146413.20_summits.bed INFO @ Mon, 12 Aug 2019 17:36:56: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (84 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。