Job ID = 9157370 sra ファイルのダウンロード中... Completed: 905276K bytes transferred in 10 seconds (728322K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 27950500 spots for /home/okishinya/chipatlas/results/ce10/SRX1132917/SRR2144366.sra Written 27950500 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:13 27950500 reads; of these: 27950500 (100.00%) were unpaired; of these: 1017301 (3.64%) aligned 0 times 23416660 (83.78%) aligned exactly 1 time 3516539 (12.58%) aligned >1 times 96.36% overall alignment rate Time searching: 00:10:13 Overall time: 00:10:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8560933 / 26933199 = 0.3179 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:48:30: # Command line: callpeak -t SRX1132917.bam -f BAM -g ce -n SRX1132917.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1132917.10 # format = BAM # ChIP-seq file = ['SRX1132917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:48:30: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:48:30: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:48:30: # Command line: callpeak -t SRX1132917.bam -f BAM -g ce -n SRX1132917.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1132917.05 # format = BAM # ChIP-seq file = ['SRX1132917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:48:30: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:48:30: # Command line: callpeak -t SRX1132917.bam -f BAM -g ce -n SRX1132917.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1132917.20 # format = BAM # ChIP-seq file = ['SRX1132917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:48:30: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:48:30: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:48:30: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:48:38: 1000000 INFO @ Tue, 27 Jun 2017 11:48:39: 1000000 INFO @ Tue, 27 Jun 2017 11:48:39: 1000000 INFO @ Tue, 27 Jun 2017 11:48:46: 2000000 INFO @ Tue, 27 Jun 2017 11:48:48: 2000000 INFO @ Tue, 27 Jun 2017 11:48:48: 2000000 INFO @ Tue, 27 Jun 2017 11:48:54: 3000000 INFO @ Tue, 27 Jun 2017 11:48:56: 3000000 INFO @ Tue, 27 Jun 2017 11:48:56: 3000000 INFO @ Tue, 27 Jun 2017 11:49:02: 4000000 INFO @ Tue, 27 Jun 2017 11:49:05: 4000000 INFO @ Tue, 27 Jun 2017 11:49:05: 4000000 INFO @ Tue, 27 Jun 2017 11:49:10: 5000000 INFO @ Tue, 27 Jun 2017 11:49:14: 5000000 INFO @ Tue, 27 Jun 2017 11:49:14: 5000000 INFO @ Tue, 27 Jun 2017 11:49:18: 6000000 INFO @ Tue, 27 Jun 2017 11:49:23: 6000000 INFO @ Tue, 27 Jun 2017 11:49:23: 6000000 INFO @ Tue, 27 Jun 2017 11:49:26: 7000000 INFO @ Tue, 27 Jun 2017 11:49:31: 7000000 INFO @ Tue, 27 Jun 2017 11:49:31: 7000000 INFO @ Tue, 27 Jun 2017 11:49:34: 8000000 INFO @ Tue, 27 Jun 2017 11:49:39: 8000000 INFO @ Tue, 27 Jun 2017 11:49:39: 8000000 INFO @ Tue, 27 Jun 2017 11:49:42: 9000000 INFO @ Tue, 27 Jun 2017 11:49:48: 9000000 INFO @ Tue, 27 Jun 2017 11:49:48: 9000000 INFO @ Tue, 27 Jun 2017 11:49:49: 10000000 INFO @ Tue, 27 Jun 2017 11:49:56: 10000000 INFO @ Tue, 27 Jun 2017 11:49:56: 10000000 INFO @ Tue, 27 Jun 2017 11:49:57: 11000000 INFO @ Tue, 27 Jun 2017 11:50:05: 12000000 INFO @ Tue, 27 Jun 2017 11:50:05: 11000000 INFO @ Tue, 27 Jun 2017 11:50:05: 11000000 INFO @ Tue, 27 Jun 2017 11:50:12: 13000000 INFO @ Tue, 27 Jun 2017 11:50:13: 12000000 INFO @ Tue, 27 Jun 2017 11:50:13: 12000000 INFO @ Tue, 27 Jun 2017 11:50:20: 14000000 INFO @ Tue, 27 Jun 2017 11:50:22: 13000000 INFO @ Tue, 27 Jun 2017 11:50:22: 13000000 INFO @ Tue, 27 Jun 2017 11:50:28: 15000000 INFO @ Tue, 27 Jun 2017 11:50:30: 14000000 INFO @ Tue, 27 Jun 2017 11:50:30: 14000000 INFO @ Tue, 27 Jun 2017 11:50:35: 16000000 INFO @ Tue, 27 Jun 2017 11:50:39: 15000000 INFO @ Tue, 27 Jun 2017 11:50:39: 15000000 INFO @ Tue, 27 Jun 2017 11:50:43: 17000000 INFO @ Tue, 27 Jun 2017 11:50:47: 16000000 INFO @ Tue, 27 Jun 2017 11:50:47: 16000000 INFO @ Tue, 27 Jun 2017 11:50:51: 18000000 INFO @ Tue, 27 Jun 2017 11:50:54: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:50:54: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:50:54: #1 total tags in treatment: 18372266 INFO @ Tue, 27 Jun 2017 11:50:54: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:50:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:50:54: #1 tags after filtering in treatment: 18372266 INFO @ Tue, 27 Jun 2017 11:50:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:50:54: #1 finished! INFO @ Tue, 27 Jun 2017 11:50:54: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:50:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:50:55: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 11:50:55: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:50:55: Process for pairing-model is terminated! cat: SRX1132917.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132917.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132917.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132917.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:50:56: 17000000 INFO @ Tue, 27 Jun 2017 11:50:56: 17000000 INFO @ Tue, 27 Jun 2017 11:51:04: 18000000 INFO @ Tue, 27 Jun 2017 11:51:04: 18000000 INFO @ Tue, 27 Jun 2017 11:51:07: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:51:07: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:51:07: #1 total tags in treatment: 18372266 INFO @ Tue, 27 Jun 2017 11:51:07: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:51:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:51:07: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:51:07: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:51:07: #1 total tags in treatment: 18372266 INFO @ Tue, 27 Jun 2017 11:51:07: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:51:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:51:08: #1 tags after filtering in treatment: 18372266 INFO @ Tue, 27 Jun 2017 11:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:51:08: #1 finished! INFO @ Tue, 27 Jun 2017 11:51:08: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:51:08: #1 tags after filtering in treatment: 18372266 INFO @ Tue, 27 Jun 2017 11:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:51:08: #1 finished! INFO @ Tue, 27 Jun 2017 11:51:08: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:51:09: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 11:51:09: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:51:09: Process for pairing-model is terminated! cat: SRX1132917.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Tue, 27 Jun 2017 11:51:09: #2 number of paired peaks: 8 WARNING @ Tue, 27 Jun 2017 11:51:09: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:51:09: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132917.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132917.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132917.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX1132917.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132917.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132917.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132917.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。