Job ID = 9157369 sra ファイルのダウンロード中... Completed: 977602K bytes transferred in 10 seconds (735121K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 29558379 spots for /home/okishinya/chipatlas/results/ce10/SRX1132916/SRR2144365.sra Written 29558379 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:51 29558379 reads; of these: 29558379 (100.00%) were unpaired; of these: 3465910 (11.73%) aligned 0 times 21930732 (74.19%) aligned exactly 1 time 4161737 (14.08%) aligned >1 times 88.27% overall alignment rate Time searching: 00:10:51 Overall time: 00:10:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10164796 / 26092469 = 0.3896 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:48:09: # Command line: callpeak -t SRX1132916.bam -f BAM -g ce -n SRX1132916.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1132916.10 # format = BAM # ChIP-seq file = ['SRX1132916.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:48:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:48:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:48:09: # Command line: callpeak -t SRX1132916.bam -f BAM -g ce -n SRX1132916.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1132916.05 # format = BAM # ChIP-seq file = ['SRX1132916.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:48:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:48:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:48:09: # Command line: callpeak -t SRX1132916.bam -f BAM -g ce -n SRX1132916.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1132916.20 # format = BAM # ChIP-seq file = ['SRX1132916.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:48:09: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:48:09: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:48:15: 1000000 INFO @ Tue, 27 Jun 2017 11:48:16: 1000000 INFO @ Tue, 27 Jun 2017 11:48:16: 1000000 INFO @ Tue, 27 Jun 2017 11:48:22: 2000000 INFO @ Tue, 27 Jun 2017 11:48:23: 2000000 INFO @ Tue, 27 Jun 2017 11:48:23: 2000000 INFO @ Tue, 27 Jun 2017 11:48:29: 3000000 INFO @ Tue, 27 Jun 2017 11:48:30: 3000000 INFO @ Tue, 27 Jun 2017 11:48:30: 3000000 INFO @ Tue, 27 Jun 2017 11:48:36: 4000000 INFO @ Tue, 27 Jun 2017 11:48:37: 4000000 INFO @ Tue, 27 Jun 2017 11:48:37: 4000000 INFO @ Tue, 27 Jun 2017 11:48:43: 5000000 INFO @ Tue, 27 Jun 2017 11:48:44: 5000000 INFO @ Tue, 27 Jun 2017 11:48:44: 5000000 INFO @ Tue, 27 Jun 2017 11:48:50: 6000000 INFO @ Tue, 27 Jun 2017 11:48:51: 6000000 INFO @ Tue, 27 Jun 2017 11:48:51: 6000000 INFO @ Tue, 27 Jun 2017 11:48:57: 7000000 INFO @ Tue, 27 Jun 2017 11:48:58: 7000000 INFO @ Tue, 27 Jun 2017 11:48:58: 7000000 INFO @ Tue, 27 Jun 2017 11:49:04: 8000000 INFO @ Tue, 27 Jun 2017 11:49:05: 8000000 INFO @ Tue, 27 Jun 2017 11:49:05: 8000000 INFO @ Tue, 27 Jun 2017 11:49:11: 9000000 INFO @ Tue, 27 Jun 2017 11:49:13: 9000000 INFO @ Tue, 27 Jun 2017 11:49:13: 9000000 INFO @ Tue, 27 Jun 2017 11:49:18: 10000000 INFO @ Tue, 27 Jun 2017 11:49:21: 10000000 INFO @ Tue, 27 Jun 2017 11:49:22: 10000000 INFO @ Tue, 27 Jun 2017 11:49:26: 11000000 INFO @ Tue, 27 Jun 2017 11:49:29: 11000000 INFO @ Tue, 27 Jun 2017 11:49:30: 11000000 INFO @ Tue, 27 Jun 2017 11:49:35: 12000000 INFO @ Tue, 27 Jun 2017 11:49:37: 12000000 INFO @ Tue, 27 Jun 2017 11:49:38: 12000000 INFO @ Tue, 27 Jun 2017 11:49:42: 13000000 INFO @ Tue, 27 Jun 2017 11:49:45: 13000000 INFO @ Tue, 27 Jun 2017 11:49:46: 13000000 INFO @ Tue, 27 Jun 2017 11:49:49: 14000000 INFO @ Tue, 27 Jun 2017 11:49:53: 14000000 INFO @ Tue, 27 Jun 2017 11:49:54: 14000000 INFO @ Tue, 27 Jun 2017 11:49:58: 15000000 INFO @ Tue, 27 Jun 2017 11:50:00: 15000000 INFO @ Tue, 27 Jun 2017 11:50:02: 15000000 INFO @ Tue, 27 Jun 2017 11:50:06: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:50:06: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:50:06: #1 total tags in treatment: 15927673 INFO @ Tue, 27 Jun 2017 11:50:06: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:50:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:50:06: #1 tags after filtering in treatment: 15927673 INFO @ Tue, 27 Jun 2017 11:50:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:50:06: #1 finished! INFO @ Tue, 27 Jun 2017 11:50:06: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:50:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:50:08: #2 number of paired peaks: 22 WARNING @ Tue, 27 Jun 2017 11:50:08: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:50:08: Process for pairing-model is terminated! cat: SRX1132916.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Tue, 27 Jun 2017 11:50:08: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:50:08: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:50:08: #1 total tags in treatment: 15927673 INFO @ Tue, 27 Jun 2017 11:50:08: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:50:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132916.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132916.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132916.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:50:08: #1 tags after filtering in treatment: 15927673 INFO @ Tue, 27 Jun 2017 11:50:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:50:08: #1 finished! INFO @ Tue, 27 Jun 2017 11:50:08: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:50:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:50:09: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:50:09: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:50:09: #1 total tags in treatment: 15927673 INFO @ Tue, 27 Jun 2017 11:50:09: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:50:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:50:09: #2 number of paired peaks: 22 WARNING @ Tue, 27 Jun 2017 11:50:09: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:50:09: Process for pairing-model is terminated! cat: SRX1132916.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132916.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132916.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132916.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:50:09: #1 tags after filtering in treatment: 15927673 INFO @ Tue, 27 Jun 2017 11:50:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:50:09: #1 finished! INFO @ Tue, 27 Jun 2017 11:50:09: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:50:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:50:11: #2 number of paired peaks: 22 WARNING @ Tue, 27 Jun 2017 11:50:11: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:50:11: Process for pairing-model is terminated! cat: SRX1132916.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132916.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132916.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132916.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。