Job ID = 9157367 sra ファイルのダウンロード中... Completed: 763403K bytes transferred in 8 seconds (737030K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 24638339 spots for /home/okishinya/chipatlas/results/ce10/SRX1132914/SRR2144363.sra Written 24638339 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:54 24638339 reads; of these: 24638339 (100.00%) were unpaired; of these: 1286860 (5.22%) aligned 0 times 20390261 (82.76%) aligned exactly 1 time 2961218 (12.02%) aligned >1 times 94.78% overall alignment rate Time searching: 00:08:54 Overall time: 00:08:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7458831 / 23351479 = 0.3194 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:44:32: # Command line: callpeak -t SRX1132914.bam -f BAM -g ce -n SRX1132914.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1132914.05 # format = BAM # ChIP-seq file = ['SRX1132914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:44:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:44:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:44:32: # Command line: callpeak -t SRX1132914.bam -f BAM -g ce -n SRX1132914.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1132914.10 # format = BAM # ChIP-seq file = ['SRX1132914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:44:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:44:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:44:32: # Command line: callpeak -t SRX1132914.bam -f BAM -g ce -n SRX1132914.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1132914.20 # format = BAM # ChIP-seq file = ['SRX1132914.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:44:32: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:44:32: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:44:40: 1000000 INFO @ Tue, 27 Jun 2017 11:44:40: 1000000 INFO @ Tue, 27 Jun 2017 11:44:41: 1000000 INFO @ Tue, 27 Jun 2017 11:44:48: 2000000 INFO @ Tue, 27 Jun 2017 11:44:49: 2000000 INFO @ Tue, 27 Jun 2017 11:44:50: 2000000 INFO @ Tue, 27 Jun 2017 11:44:56: 3000000 INFO @ Tue, 27 Jun 2017 11:44:57: 3000000 INFO @ Tue, 27 Jun 2017 11:44:59: 3000000 INFO @ Tue, 27 Jun 2017 11:45:04: 4000000 INFO @ Tue, 27 Jun 2017 11:45:05: 4000000 INFO @ Tue, 27 Jun 2017 11:45:09: 4000000 INFO @ Tue, 27 Jun 2017 11:45:12: 5000000 INFO @ Tue, 27 Jun 2017 11:45:14: 5000000 INFO @ Tue, 27 Jun 2017 11:45:18: 5000000 INFO @ Tue, 27 Jun 2017 11:45:20: 6000000 INFO @ Tue, 27 Jun 2017 11:45:22: 6000000 INFO @ Tue, 27 Jun 2017 11:45:27: 6000000 INFO @ Tue, 27 Jun 2017 11:45:28: 7000000 INFO @ Tue, 27 Jun 2017 11:45:31: 7000000 INFO @ Tue, 27 Jun 2017 11:45:35: 7000000 INFO @ Tue, 27 Jun 2017 11:45:38: 8000000 INFO @ Tue, 27 Jun 2017 11:45:40: 8000000 INFO @ Tue, 27 Jun 2017 11:45:43: 8000000 INFO @ Tue, 27 Jun 2017 11:45:48: 9000000 INFO @ Tue, 27 Jun 2017 11:45:50: 9000000 INFO @ Tue, 27 Jun 2017 11:45:51: 9000000 INFO @ Tue, 27 Jun 2017 11:45:57: 10000000 INFO @ Tue, 27 Jun 2017 11:45:59: 10000000 INFO @ Tue, 27 Jun 2017 11:46:00: 10000000 INFO @ Tue, 27 Jun 2017 11:46:07: 11000000 INFO @ Tue, 27 Jun 2017 11:46:07: 11000000 INFO @ Tue, 27 Jun 2017 11:46:10: 11000000 INFO @ Tue, 27 Jun 2017 11:46:15: 12000000 INFO @ Tue, 27 Jun 2017 11:46:17: 12000000 INFO @ Tue, 27 Jun 2017 11:46:19: 12000000 INFO @ Tue, 27 Jun 2017 11:46:24: 13000000 INFO @ Tue, 27 Jun 2017 11:46:26: 13000000 INFO @ Tue, 27 Jun 2017 11:46:28: 13000000 INFO @ Tue, 27 Jun 2017 11:46:32: 14000000 INFO @ Tue, 27 Jun 2017 11:46:35: 14000000 INFO @ Tue, 27 Jun 2017 11:46:37: 14000000 INFO @ Tue, 27 Jun 2017 11:46:40: 15000000 INFO @ Tue, 27 Jun 2017 11:46:44: 15000000 INFO @ Tue, 27 Jun 2017 11:46:46: 15000000 INFO @ Tue, 27 Jun 2017 11:46:47: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:46:47: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:46:47: #1 total tags in treatment: 15892648 INFO @ Tue, 27 Jun 2017 11:46:47: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:46:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:46:48: #1 tags after filtering in treatment: 15892648 INFO @ Tue, 27 Jun 2017 11:46:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:46:48: #1 finished! INFO @ Tue, 27 Jun 2017 11:46:48: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:46:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:46:49: #2 number of paired peaks: 9 WARNING @ Tue, 27 Jun 2017 11:46:49: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:46:49: Process for pairing-model is terminated! cat: SRX1132914.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 7 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132914.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132914.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132914.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:46:52: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:46:52: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:46:52: #1 total tags in treatment: 15892648 INFO @ Tue, 27 Jun 2017 11:46:52: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:46:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:46:52: #1 tags after filtering in treatment: 15892648 INFO @ Tue, 27 Jun 2017 11:46:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:46:52: #1 finished! INFO @ Tue, 27 Jun 2017 11:46:52: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:46:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:46:53: #2 number of paired peaks: 9 WARNING @ Tue, 27 Jun 2017 11:46:53: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:46:53: Process for pairing-model is terminated! cat: SRX1132914.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132914.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132914.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132914.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:46:54: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:46:54: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:46:54: #1 total tags in treatment: 15892648 INFO @ Tue, 27 Jun 2017 11:46:54: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:46:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:46:54: #1 tags after filtering in treatment: 15892648 INFO @ Tue, 27 Jun 2017 11:46:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:46:54: #1 finished! INFO @ Tue, 27 Jun 2017 11:46:54: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:46:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:46:55: #2 number of paired peaks: 9 WARNING @ Tue, 27 Jun 2017 11:46:55: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:46:55: Process for pairing-model is terminated! cat: SRX1132914.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132914.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132914.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132914.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。