Job ID = 2589353 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 36,011,940 reads read : 36,011,940 reads written : 36,011,940 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:36 36011940 reads; of these: 36011940 (100.00%) were unpaired; of these: 664063 (1.84%) aligned 0 times 29503323 (81.93%) aligned exactly 1 time 5844554 (16.23%) aligned >1 times 98.16% overall alignment rate Time searching: 00:09:36 Overall time: 00:09:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5867081 / 35347877 = 0.1660 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:10:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:10:52: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:10:52: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:10:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:10:52: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:10:52: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:10:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:10:54: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:10:54: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:11:01: 1000000 INFO @ Mon, 12 Aug 2019 18:11:02: 1000000 INFO @ Mon, 12 Aug 2019 18:11:04: 1000000 INFO @ Mon, 12 Aug 2019 18:11:09: 2000000 INFO @ Mon, 12 Aug 2019 18:11:12: 2000000 INFO @ Mon, 12 Aug 2019 18:11:13: 2000000 INFO @ Mon, 12 Aug 2019 18:11:17: 3000000 INFO @ Mon, 12 Aug 2019 18:11:21: 3000000 INFO @ Mon, 12 Aug 2019 18:11:23: 3000000 INFO @ Mon, 12 Aug 2019 18:11:25: 4000000 INFO @ Mon, 12 Aug 2019 18:11:31: 4000000 INFO @ Mon, 12 Aug 2019 18:11:33: 4000000 INFO @ Mon, 12 Aug 2019 18:11:33: 5000000 INFO @ Mon, 12 Aug 2019 18:11:41: 5000000 INFO @ Mon, 12 Aug 2019 18:11:42: 6000000 INFO @ Mon, 12 Aug 2019 18:11:43: 5000000 INFO @ Mon, 12 Aug 2019 18:11:52: 6000000 INFO @ Mon, 12 Aug 2019 18:11:52: 7000000 INFO @ Mon, 12 Aug 2019 18:11:55: 6000000 INFO @ Mon, 12 Aug 2019 18:12:00: 8000000 INFO @ Mon, 12 Aug 2019 18:12:01: 7000000 INFO @ Mon, 12 Aug 2019 18:12:05: 7000000 INFO @ Mon, 12 Aug 2019 18:12:08: 9000000 INFO @ Mon, 12 Aug 2019 18:12:11: 8000000 INFO @ Mon, 12 Aug 2019 18:12:15: 8000000 INFO @ Mon, 12 Aug 2019 18:12:16: 10000000 INFO @ Mon, 12 Aug 2019 18:12:21: 9000000 INFO @ Mon, 12 Aug 2019 18:12:24: 11000000 INFO @ Mon, 12 Aug 2019 18:12:25: 9000000 INFO @ Mon, 12 Aug 2019 18:12:31: 10000000 INFO @ Mon, 12 Aug 2019 18:12:32: 12000000 INFO @ Mon, 12 Aug 2019 18:12:35: 10000000 INFO @ Mon, 12 Aug 2019 18:12:40: 13000000 INFO @ Mon, 12 Aug 2019 18:12:41: 11000000 INFO @ Mon, 12 Aug 2019 18:12:44: 11000000 INFO @ Mon, 12 Aug 2019 18:12:48: 14000000 INFO @ Mon, 12 Aug 2019 18:12:51: 12000000 INFO @ Mon, 12 Aug 2019 18:12:54: 12000000 INFO @ Mon, 12 Aug 2019 18:12:56: 15000000 INFO @ Mon, 12 Aug 2019 18:13:01: 13000000 INFO @ Mon, 12 Aug 2019 18:13:04: 16000000 INFO @ Mon, 12 Aug 2019 18:13:04: 13000000 INFO @ Mon, 12 Aug 2019 18:13:11: 14000000 INFO @ Mon, 12 Aug 2019 18:13:12: 17000000 INFO @ Mon, 12 Aug 2019 18:13:14: 14000000 INFO @ Mon, 12 Aug 2019 18:13:20: 18000000 INFO @ Mon, 12 Aug 2019 18:13:21: 15000000 INFO @ Mon, 12 Aug 2019 18:13:24: 15000000 INFO @ Mon, 12 Aug 2019 18:13:28: 19000000 INFO @ Mon, 12 Aug 2019 18:13:30: 16000000 INFO @ Mon, 12 Aug 2019 18:13:34: 16000000 INFO @ Mon, 12 Aug 2019 18:13:36: 20000000 INFO @ Mon, 12 Aug 2019 18:13:40: 17000000 INFO @ Mon, 12 Aug 2019 18:13:44: 21000000 INFO @ Mon, 12 Aug 2019 18:13:44: 17000000 INFO @ Mon, 12 Aug 2019 18:13:50: 18000000 INFO @ Mon, 12 Aug 2019 18:13:51: 22000000 INFO @ Mon, 12 Aug 2019 18:13:53: 18000000 INFO @ Mon, 12 Aug 2019 18:13:59: 23000000 INFO @ Mon, 12 Aug 2019 18:13:59: 19000000 INFO @ Mon, 12 Aug 2019 18:14:03: 19000000 INFO @ Mon, 12 Aug 2019 18:14:07: 24000000 INFO @ Mon, 12 Aug 2019 18:14:09: 20000000 INFO @ Mon, 12 Aug 2019 18:14:13: 20000000 INFO @ Mon, 12 Aug 2019 18:14:15: 25000000 INFO @ Mon, 12 Aug 2019 18:14:18: 21000000 INFO @ Mon, 12 Aug 2019 18:14:22: 21000000 INFO @ Mon, 12 Aug 2019 18:14:23: 26000000 INFO @ Mon, 12 Aug 2019 18:14:28: 22000000 INFO @ Mon, 12 Aug 2019 18:14:31: 27000000 INFO @ Mon, 12 Aug 2019 18:14:32: 22000000 INFO @ Mon, 12 Aug 2019 18:14:37: 23000000 INFO @ Mon, 12 Aug 2019 18:14:39: 28000000 INFO @ Mon, 12 Aug 2019 18:14:41: 23000000 INFO @ Mon, 12 Aug 2019 18:14:47: 29000000 INFO @ Mon, 12 Aug 2019 18:14:47: 24000000 INFO @ Mon, 12 Aug 2019 18:14:50: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 18:14:50: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 18:14:50: #1 total tags in treatment: 29480796 INFO @ Mon, 12 Aug 2019 18:14:50: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:14:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:14:51: 24000000 INFO @ Mon, 12 Aug 2019 18:14:51: #1 tags after filtering in treatment: 29480796 INFO @ Mon, 12 Aug 2019 18:14:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:14:51: #1 finished! INFO @ Mon, 12 Aug 2019 18:14:51: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:14:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:14:53: #2 number of paired peaks: 86 WARNING @ Mon, 12 Aug 2019 18:14:53: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 18:14:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:14:56: 25000000 INFO @ Mon, 12 Aug 2019 18:15:00: 25000000 INFO @ Mon, 12 Aug 2019 18:15:06: 26000000 INFO @ Mon, 12 Aug 2019 18:15:09: 26000000 INFO @ Mon, 12 Aug 2019 18:15:15: 27000000 INFO @ Mon, 12 Aug 2019 18:15:19: 27000000 INFO @ Mon, 12 Aug 2019 18:15:25: 28000000 INFO @ Mon, 12 Aug 2019 18:15:29: 28000000 INFO @ Mon, 12 Aug 2019 18:15:35: 29000000 INFO @ Mon, 12 Aug 2019 18:15:38: 29000000 INFO @ Mon, 12 Aug 2019 18:15:40: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 18:15:40: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 18:15:40: #1 total tags in treatment: 29480796 INFO @ Mon, 12 Aug 2019 18:15:40: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:15:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:15:40: #1 tags after filtering in treatment: 29480796 INFO @ Mon, 12 Aug 2019 18:15:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:15:40: #1 finished! INFO @ Mon, 12 Aug 2019 18:15:40: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:15:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:15:43: #2 number of paired peaks: 86 WARNING @ Mon, 12 Aug 2019 18:15:43: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 18:15:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:15:43: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 18:15:43: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 18:15:43: #1 total tags in treatment: 29480796 INFO @ Mon, 12 Aug 2019 18:15:43: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:15:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:15:44: #1 tags after filtering in treatment: 29480796 INFO @ Mon, 12 Aug 2019 18:15:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:15:44: #1 finished! INFO @ Mon, 12 Aug 2019 18:15:44: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:15:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:15:46: #2 number of paired peaks: 86 WARNING @ Mon, 12 Aug 2019 18:15:46: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 18:15:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078706/SRX1078706.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。