Job ID = 9157360 sra ファイルのダウンロード中... Completed: 395620K bytes transferred in 6 seconds (529742K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 24819951 spots for /home/okishinya/chipatlas/results/ce10/SRX1030170/SRR2029636.sra Written 24819951 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:59 24819951 reads; of these: 24819951 (100.00%) were unpaired; of these: 7266 (0.03%) aligned 0 times 23079956 (92.99%) aligned exactly 1 time 1732729 (6.98%) aligned >1 times 99.97% overall alignment rate Time searching: 00:04:59 Overall time: 00:04:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1013361 / 24812685 = 0.0408 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:38:54: # Command line: callpeak -t SRX1030170.bam -f BAM -g ce -n SRX1030170.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1030170.10 # format = BAM # ChIP-seq file = ['SRX1030170.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:38:54: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:38:54: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:38:54: # Command line: callpeak -t SRX1030170.bam -f BAM -g ce -n SRX1030170.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1030170.20 # format = BAM # ChIP-seq file = ['SRX1030170.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:38:54: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:38:54: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:38:54: # Command line: callpeak -t SRX1030170.bam -f BAM -g ce -n SRX1030170.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1030170.05 # format = BAM # ChIP-seq file = ['SRX1030170.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:38:54: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:38:54: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:39:01: 1000000 INFO @ Tue, 27 Jun 2017 11:39:01: 1000000 INFO @ Tue, 27 Jun 2017 11:39:01: 1000000 INFO @ Tue, 27 Jun 2017 11:39:07: 2000000 INFO @ Tue, 27 Jun 2017 11:39:07: 2000000 INFO @ Tue, 27 Jun 2017 11:39:08: 2000000 INFO @ Tue, 27 Jun 2017 11:39:14: 3000000 INFO @ Tue, 27 Jun 2017 11:39:14: 3000000 INFO @ Tue, 27 Jun 2017 11:39:15: 3000000 INFO @ Tue, 27 Jun 2017 11:39:21: 4000000 INFO @ Tue, 27 Jun 2017 11:39:21: 4000000 INFO @ Tue, 27 Jun 2017 11:39:22: 4000000 INFO @ Tue, 27 Jun 2017 11:39:27: 5000000 INFO @ Tue, 27 Jun 2017 11:39:28: 5000000 INFO @ Tue, 27 Jun 2017 11:39:29: 5000000 INFO @ Tue, 27 Jun 2017 11:39:35: 6000000 INFO @ Tue, 27 Jun 2017 11:39:35: 6000000 INFO @ Tue, 27 Jun 2017 11:39:36: 6000000 INFO @ Tue, 27 Jun 2017 11:39:41: 7000000 INFO @ Tue, 27 Jun 2017 11:39:42: 7000000 INFO @ Tue, 27 Jun 2017 11:39:44: 7000000 INFO @ Tue, 27 Jun 2017 11:39:48: 8000000 INFO @ Tue, 27 Jun 2017 11:39:49: 8000000 INFO @ Tue, 27 Jun 2017 11:39:51: 8000000 INFO @ Tue, 27 Jun 2017 11:39:55: 9000000 INFO @ Tue, 27 Jun 2017 11:39:56: 9000000 INFO @ Tue, 27 Jun 2017 11:39:58: 9000000 INFO @ Tue, 27 Jun 2017 11:40:01: 10000000 INFO @ Tue, 27 Jun 2017 11:40:02: 10000000 INFO @ Tue, 27 Jun 2017 11:40:05: 10000000 INFO @ Tue, 27 Jun 2017 11:40:07: 11000000 INFO @ Tue, 27 Jun 2017 11:40:09: 11000000 INFO @ Tue, 27 Jun 2017 11:40:12: 11000000 INFO @ Tue, 27 Jun 2017 11:40:14: 12000000 INFO @ Tue, 27 Jun 2017 11:40:16: 12000000 INFO @ Tue, 27 Jun 2017 11:40:19: 12000000 INFO @ Tue, 27 Jun 2017 11:40:20: 13000000 INFO @ Tue, 27 Jun 2017 11:40:23: 13000000 INFO @ Tue, 27 Jun 2017 11:40:26: 13000000 INFO @ Tue, 27 Jun 2017 11:40:27: 14000000 INFO @ Tue, 27 Jun 2017 11:40:30: 14000000 INFO @ Tue, 27 Jun 2017 11:40:33: 14000000 INFO @ Tue, 27 Jun 2017 11:40:33: 15000000 INFO @ Tue, 27 Jun 2017 11:40:37: 15000000 INFO @ Tue, 27 Jun 2017 11:40:40: 16000000 INFO @ Tue, 27 Jun 2017 11:40:40: 15000000 INFO @ Tue, 27 Jun 2017 11:40:43: 16000000 INFO @ Tue, 27 Jun 2017 11:40:46: 17000000 INFO @ Tue, 27 Jun 2017 11:40:47: 16000000 INFO @ Tue, 27 Jun 2017 11:40:50: 17000000 INFO @ Tue, 27 Jun 2017 11:40:53: 18000000 INFO @ Tue, 27 Jun 2017 11:40:55: 17000000 INFO @ Tue, 27 Jun 2017 11:40:57: 18000000 INFO @ Tue, 27 Jun 2017 11:41:00: 19000000 INFO @ Tue, 27 Jun 2017 11:41:02: 18000000 INFO @ Tue, 27 Jun 2017 11:41:04: 19000000 INFO @ Tue, 27 Jun 2017 11:41:06: 20000000 INFO @ Tue, 27 Jun 2017 11:41:09: 19000000 INFO @ Tue, 27 Jun 2017 11:41:11: 20000000 INFO @ Tue, 27 Jun 2017 11:41:13: 21000000 INFO @ Tue, 27 Jun 2017 11:41:16: 20000000 INFO @ Tue, 27 Jun 2017 11:41:18: 21000000 INFO @ Tue, 27 Jun 2017 11:41:19: 22000000 INFO @ Tue, 27 Jun 2017 11:41:23: 21000000 INFO @ Tue, 27 Jun 2017 11:41:24: 22000000 INFO @ Tue, 27 Jun 2017 11:41:25: 23000000 INFO @ Tue, 27 Jun 2017 11:41:30: 22000000 INFO @ Tue, 27 Jun 2017 11:41:31: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:41:31: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:41:31: #1 total tags in treatment: 23799324 INFO @ Tue, 27 Jun 2017 11:41:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:41:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:41:31: 23000000 INFO @ Tue, 27 Jun 2017 11:41:31: #1 tags after filtering in treatment: 23799324 INFO @ Tue, 27 Jun 2017 11:41:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:41:31: #1 finished! INFO @ Tue, 27 Jun 2017 11:41:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:41:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:41:33: #2 number of paired peaks: 6 WARNING @ Tue, 27 Jun 2017 11:41:33: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:41:33: Process for pairing-model is terminated! cat: SRX1030170.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1030170.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030170.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030170.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:41:36: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:41:36: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:41:36: #1 total tags in treatment: 23799324 INFO @ Tue, 27 Jun 2017 11:41:36: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:41:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:41:37: 23000000 INFO @ Tue, 27 Jun 2017 11:41:37: #1 tags after filtering in treatment: 23799324 INFO @ Tue, 27 Jun 2017 11:41:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:41:37: #1 finished! INFO @ Tue, 27 Jun 2017 11:41:37: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:41:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:41:39: #2 number of paired peaks: 6 WARNING @ Tue, 27 Jun 2017 11:41:39: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:41:39: Process for pairing-model is terminated! cat: SRX1030170.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1030170.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030170.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030170.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:41:42: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:41:42: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:41:42: #1 total tags in treatment: 23799324 INFO @ Tue, 27 Jun 2017 11:41:42: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:41:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:41:43: #1 tags after filtering in treatment: 23799324 INFO @ Tue, 27 Jun 2017 11:41:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:41:43: #1 finished! INFO @ Tue, 27 Jun 2017 11:41:43: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:41:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:41:44: #2 number of paired peaks: 6 WARNING @ Tue, 27 Jun 2017 11:41:44: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:41:44: Process for pairing-model is terminated! cat: SRX1030170.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1030170.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030170.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1030170.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。