Job ID = 2237040 sra ファイルのダウンロード中... Completed: 67941K bytes transferred in 5 seconds (110377K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 13456 0 13456 0 0 25244 0 --:--:-- --:--:-- --:--:-- 39230 100 38046 0 38046 0 0 52658 0 --:--:-- --:--:-- --:--:-- 71515 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 2772587 spots for /home/okishinya/chipatlas/results/ce10/SRX080095/SRR298915.sra Written 2772587 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:29 2772587 reads; of these: 2772587 (100.00%) were unpaired; of these: 250317 (9.03%) aligned 0 times 2121499 (76.52%) aligned exactly 1 time 400771 (14.45%) aligned >1 times 90.97% overall alignment rate Time searching: 00:00:29 Overall time: 00:00:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 125469 / 2522270 = 0.0497 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 30 Apr 2015 11:25:26: # Command line: callpeak -t SRX080095.bam -f BAM -g ce -n SRX080095.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX080095.05 # format = BAM # ChIP-seq file = ['SRX080095.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:26: # Command line: callpeak -t SRX080095.bam -f BAM -g ce -n SRX080095.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX080095.20 # format = BAM # ChIP-seq file = ['SRX080095.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:26: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:26: # Command line: callpeak -t SRX080095.bam -f BAM -g ce -n SRX080095.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX080095.10 # format = BAM # ChIP-seq file = ['SRX080095.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:26: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:26: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:26: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:26: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:26: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:30: 1000000 INFO @ Thu, 30 Apr 2015 11:25:30: 1000000 INFO @ Thu, 30 Apr 2015 11:25:31: 1000000 INFO @ Thu, 30 Apr 2015 11:25:35: 2000000 INFO @ Thu, 30 Apr 2015 11:25:35: 2000000 INFO @ Thu, 30 Apr 2015 11:25:36: 2000000 INFO @ Thu, 30 Apr 2015 11:25:37: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:25:37: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:25:37: #1 total tags in treatment: 2396801 INFO @ Thu, 30 Apr 2015 11:25:37: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:25:38: #1 total tags in treatment: 2396801 INFO @ Thu, 30 Apr 2015 11:25:38: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:38: #1 tags after filtering in treatment: 2396735 INFO @ Thu, 30 Apr 2015 11:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:38: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:38: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:38: #1 tags after filtering in treatment: 2396735 INFO @ Thu, 30 Apr 2015 11:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:38: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:38: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:25:38: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:25:38: #1 total tags in treatment: 2396801 INFO @ Thu, 30 Apr 2015 11:25:38: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:38: #2 number of paired peaks: 462 WARNING @ Thu, 30 Apr 2015 11:25:38: Fewer paired peaks (462) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 462 pairs to build model! INFO @ Thu, 30 Apr 2015 11:25:38: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:38: #1 tags after filtering in treatment: 2396735 INFO @ Thu, 30 Apr 2015 11:25:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:38: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:38: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:38: #2 number of paired peaks: 462 WARNING @ Thu, 30 Apr 2015 11:25:38: Fewer paired peaks (462) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 462 pairs to build model! INFO @ Thu, 30 Apr 2015 11:25:38: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:39: #2 number of paired peaks: 462 WARNING @ Thu, 30 Apr 2015 11:25:39: Fewer paired peaks (462) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 462 pairs to build model! INFO @ Thu, 30 Apr 2015 11:25:39: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:40: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:40: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:40: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:40: #2 predicted fragment length is 30 bps INFO @ Thu, 30 Apr 2015 11:25:40: #2 alternative fragment length(s) may be 30,547,577 bps INFO @ Thu, 30 Apr 2015 11:25:40: #2.2 Generate R script for model : SRX080095.20_model.r WARNING @ Thu, 30 Apr 2015 11:25:40: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 30 Apr 2015 11:25:40: #2 You may need to consider one of the other alternative d(s): 30,547,577 WARNING @ Thu, 30 Apr 2015 11:25:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 30 Apr 2015 11:25:40: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:40: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:25:40: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:40: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:40: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:40: #2 predicted fragment length is 30 bps INFO @ Thu, 30 Apr 2015 11:25:40: #2 alternative fragment length(s) may be 30,547,577 bps INFO @ Thu, 30 Apr 2015 11:25:40: #2.2 Generate R script for model : SRX080095.05_model.r WARNING @ Thu, 30 Apr 2015 11:25:40: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 30 Apr 2015 11:25:40: #2 You may need to consider one of the other alternative d(s): 30,547,577 WARNING @ Thu, 30 Apr 2015 11:25:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 30 Apr 2015 11:25:40: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:40: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:25:41: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:41: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:41: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:41: #2 predicted fragment length is 30 bps INFO @ Thu, 30 Apr 2015 11:25:41: #2 alternative fragment length(s) may be 30,547,577 bps INFO @ Thu, 30 Apr 2015 11:25:41: #2.2 Generate R script for model : SRX080095.10_model.r WARNING @ Thu, 30 Apr 2015 11:25:41: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 30 Apr 2015 11:25:41: #2 You may need to consider one of the other alternative d(s): 30,547,577 WARNING @ Thu, 30 Apr 2015 11:25:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 30 Apr 2015 11:25:41: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:41: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:25:54: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:25:55: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:25:55: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:26:04: #4 Write output xls file... SRX080095.20_peaks.xls INFO @ Thu, 30 Apr 2015 11:26:04: #4 Write peak in narrowPeak format file... SRX080095.20_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:26:04: #4 Write summits bed file... SRX080095.20_summits.bed INFO @ Thu, 30 Apr 2015 11:26:04: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (23 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 30 Apr 2015 11:26:05: #4 Write output xls file... SRX080095.05_peaks.xls INFO @ Thu, 30 Apr 2015 11:26:05: #4 Write peak in narrowPeak format file... SRX080095.05_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:26:05: #4 Write summits bed file... SRX080095.05_summits.bed INFO @ Thu, 30 Apr 2015 11:26:05: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (319 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 30 Apr 2015 11:26:05: #4 Write output xls file... SRX080095.10_peaks.xls INFO @ Thu, 30 Apr 2015 11:26:05: #4 Write peak in narrowPeak format file... SRX080095.10_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:26:05: #4 Write summits bed file... SRX080095.10_summits.bed INFO @ Thu, 30 Apr 2015 11:26:05: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (137 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。