Job ID = 2589294 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,134,187 reads read : 5,134,187 reads written : 5,134,187 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR217367.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:33 5134187 reads; of these: 5134187 (100.00%) were unpaired; of these: 3413852 (66.49%) aligned 0 times 1492580 (29.07%) aligned exactly 1 time 227755 (4.44%) aligned >1 times 33.51% overall alignment rate Time searching: 00:00:33 Overall time: 00:00:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 140721 / 1720335 = 0.0818 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:21:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:21:03: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:21:03: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:21:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:21:04: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:21:04: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:21:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:21:05: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:21:05: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:21:10: 1000000 INFO @ Mon, 12 Aug 2019 17:21:11: 1000000 INFO @ Mon, 12 Aug 2019 17:21:13: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:21:13: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:21:13: #1 total tags in treatment: 1579614 INFO @ Mon, 12 Aug 2019 17:21:13: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:21:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:21:13: #1 tags after filtering in treatment: 1579614 INFO @ Mon, 12 Aug 2019 17:21:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:21:13: #1 finished! INFO @ Mon, 12 Aug 2019 17:21:13: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:21:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:21:14: #2 number of paired peaks: 1988 INFO @ Mon, 12 Aug 2019 17:21:14: start model_add_line... INFO @ Mon, 12 Aug 2019 17:21:14: start X-correlation... INFO @ Mon, 12 Aug 2019 17:21:14: end of X-cor INFO @ Mon, 12 Aug 2019 17:21:14: #2 finished! INFO @ Mon, 12 Aug 2019 17:21:14: #2 predicted fragment length is 146 bps INFO @ Mon, 12 Aug 2019 17:21:14: #2 alternative fragment length(s) may be 146 bps INFO @ Mon, 12 Aug 2019 17:21:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.05_model.r INFO @ Mon, 12 Aug 2019 17:21:14: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:21:14: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:21:14: 1000000 INFO @ Mon, 12 Aug 2019 17:21:14: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:21:14: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:21:14: #1 total tags in treatment: 1579614 INFO @ Mon, 12 Aug 2019 17:21:14: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:21:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:21:14: #1 tags after filtering in treatment: 1579614 INFO @ Mon, 12 Aug 2019 17:21:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:21:14: #1 finished! INFO @ Mon, 12 Aug 2019 17:21:14: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:21:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:21:15: #2 number of paired peaks: 1988 INFO @ Mon, 12 Aug 2019 17:21:15: start model_add_line... INFO @ Mon, 12 Aug 2019 17:21:15: start X-correlation... INFO @ Mon, 12 Aug 2019 17:21:15: end of X-cor INFO @ Mon, 12 Aug 2019 17:21:15: #2 finished! INFO @ Mon, 12 Aug 2019 17:21:15: #2 predicted fragment length is 146 bps INFO @ Mon, 12 Aug 2019 17:21:15: #2 alternative fragment length(s) may be 146 bps INFO @ Mon, 12 Aug 2019 17:21:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.10_model.r INFO @ Mon, 12 Aug 2019 17:21:17: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:21:17: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:21:19: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:21:19: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:21:19: #1 total tags in treatment: 1579614 INFO @ Mon, 12 Aug 2019 17:21:19: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:21:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:21:19: #1 tags after filtering in treatment: 1579614 INFO @ Mon, 12 Aug 2019 17:21:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:21:19: #1 finished! INFO @ Mon, 12 Aug 2019 17:21:19: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:21:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:21:19: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:21:19: #2 number of paired peaks: 1988 INFO @ Mon, 12 Aug 2019 17:21:19: start model_add_line... INFO @ Mon, 12 Aug 2019 17:21:19: start X-correlation... INFO @ Mon, 12 Aug 2019 17:21:19: end of X-cor INFO @ Mon, 12 Aug 2019 17:21:19: #2 finished! INFO @ Mon, 12 Aug 2019 17:21:19: #2 predicted fragment length is 146 bps INFO @ Mon, 12 Aug 2019 17:21:19: #2 alternative fragment length(s) may be 146 bps INFO @ Mon, 12 Aug 2019 17:21:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.20_model.r INFO @ Mon, 12 Aug 2019 17:21:19: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:21:19: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:21:21: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:21:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:21:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.05_summits.bed INFO @ Mon, 12 Aug 2019 17:21:21: Done! pass1 - making usageList (7 chroms): 2 millis INFO @ Mon, 12 Aug 2019 17:21:22: #3 Call peaks for each chromosome... pass2 - checking and writing primary data (3947 records, 4 fields): 1149 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:21:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:21:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:21:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.10_summits.bed INFO @ Mon, 12 Aug 2019 17:21:24: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1688 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:21:24: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:21:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:21:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:21:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065661/SRX065661.20_summits.bed INFO @ Mon, 12 Aug 2019 17:21:27: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (425 records, 4 fields): 797 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。