Job ID = 2589256 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,938,307 reads read : 5,938,307 reads written : 5,938,307 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR217332.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:01 5938307 reads; of these: 5938307 (100.00%) were unpaired; of these: 104309 (1.76%) aligned 0 times 5080122 (85.55%) aligned exactly 1 time 753876 (12.70%) aligned >1 times 98.24% overall alignment rate Time searching: 00:01:01 Overall time: 00:01:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 694080 / 5833998 = 0.1190 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:17:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:17:22: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:17:22: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:17:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:17:23: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:17:23: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:17:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:17:24: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:17:24: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:17:32: 1000000 INFO @ Mon, 12 Aug 2019 17:17:32: 1000000 INFO @ Mon, 12 Aug 2019 17:17:33: 1000000 INFO @ Mon, 12 Aug 2019 17:17:39: 2000000 INFO @ Mon, 12 Aug 2019 17:17:43: 2000000 INFO @ Mon, 12 Aug 2019 17:17:44: 2000000 INFO @ Mon, 12 Aug 2019 17:17:47: 3000000 INFO @ Mon, 12 Aug 2019 17:17:53: 3000000 INFO @ Mon, 12 Aug 2019 17:17:54: 4000000 INFO @ Mon, 12 Aug 2019 17:17:54: 3000000 INFO @ Mon, 12 Aug 2019 17:18:01: 5000000 INFO @ Mon, 12 Aug 2019 17:18:02: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:18:02: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:18:02: #1 total tags in treatment: 5139918 INFO @ Mon, 12 Aug 2019 17:18:02: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:18:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:18:02: #1 tags after filtering in treatment: 5139918 INFO @ Mon, 12 Aug 2019 17:18:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:18:02: #1 finished! INFO @ Mon, 12 Aug 2019 17:18:02: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:18:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:18:03: 4000000 INFO @ Mon, 12 Aug 2019 17:18:03: #2 number of paired peaks: 282 WARNING @ Mon, 12 Aug 2019 17:18:03: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Mon, 12 Aug 2019 17:18:03: start model_add_line... INFO @ Mon, 12 Aug 2019 17:18:03: start X-correlation... INFO @ Mon, 12 Aug 2019 17:18:03: end of X-cor INFO @ Mon, 12 Aug 2019 17:18:03: #2 finished! INFO @ Mon, 12 Aug 2019 17:18:03: #2 predicted fragment length is 33 bps INFO @ Mon, 12 Aug 2019 17:18:03: #2 alternative fragment length(s) may be 4,33,80,156,505,582 bps INFO @ Mon, 12 Aug 2019 17:18:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.20_model.r WARNING @ Mon, 12 Aug 2019 17:18:03: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:18:03: #2 You may need to consider one of the other alternative d(s): 4,33,80,156,505,582 WARNING @ Mon, 12 Aug 2019 17:18:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:18:03: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:18:03: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:18:04: 4000000 INFO @ Mon, 12 Aug 2019 17:18:12: 5000000 INFO @ Mon, 12 Aug 2019 17:18:13: 5000000 INFO @ Mon, 12 Aug 2019 17:18:14: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:18:14: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:18:14: #1 total tags in treatment: 5139918 INFO @ Mon, 12 Aug 2019 17:18:14: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:18:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:18:14: #1 tags after filtering in treatment: 5139918 INFO @ Mon, 12 Aug 2019 17:18:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:18:14: #1 finished! INFO @ Mon, 12 Aug 2019 17:18:14: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:18:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:18:14: #2 number of paired peaks: 282 WARNING @ Mon, 12 Aug 2019 17:18:14: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Mon, 12 Aug 2019 17:18:14: start model_add_line... INFO @ Mon, 12 Aug 2019 17:18:14: start X-correlation... INFO @ Mon, 12 Aug 2019 17:18:14: end of X-cor INFO @ Mon, 12 Aug 2019 17:18:14: #2 finished! INFO @ Mon, 12 Aug 2019 17:18:14: #2 predicted fragment length is 33 bps INFO @ Mon, 12 Aug 2019 17:18:14: #2 alternative fragment length(s) may be 4,33,80,156,505,582 bps INFO @ Mon, 12 Aug 2019 17:18:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.05_model.r WARNING @ Mon, 12 Aug 2019 17:18:14: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:18:14: #2 You may need to consider one of the other alternative d(s): 4,33,80,156,505,582 WARNING @ Mon, 12 Aug 2019 17:18:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:18:14: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:18:14: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:18:14: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:18:14: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:18:14: #1 total tags in treatment: 5139918 INFO @ Mon, 12 Aug 2019 17:18:14: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:18:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:18:15: #1 tags after filtering in treatment: 5139918 INFO @ Mon, 12 Aug 2019 17:18:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:18:15: #1 finished! INFO @ Mon, 12 Aug 2019 17:18:15: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:18:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:18:15: #2 number of paired peaks: 282 WARNING @ Mon, 12 Aug 2019 17:18:15: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Mon, 12 Aug 2019 17:18:15: start model_add_line... INFO @ Mon, 12 Aug 2019 17:18:15: start X-correlation... INFO @ Mon, 12 Aug 2019 17:18:15: end of X-cor INFO @ Mon, 12 Aug 2019 17:18:15: #2 finished! INFO @ Mon, 12 Aug 2019 17:18:15: #2 predicted fragment length is 33 bps INFO @ Mon, 12 Aug 2019 17:18:15: #2 alternative fragment length(s) may be 4,33,80,156,505,582 bps INFO @ Mon, 12 Aug 2019 17:18:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.10_model.r WARNING @ Mon, 12 Aug 2019 17:18:15: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:18:15: #2 You may need to consider one of the other alternative d(s): 4,33,80,156,505,582 WARNING @ Mon, 12 Aug 2019 17:18:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:18:15: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:18:15: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:18:17: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:18:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:18:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:18:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.20_summits.bed INFO @ Mon, 12 Aug 2019 17:18:25: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (24 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:18:29: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:18:30: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:18:36: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:18:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:18:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.05_summits.bed INFO @ Mon, 12 Aug 2019 17:18:36: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (447 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:18:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:18:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:18:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065626/SRX065626.10_summits.bed INFO @ Mon, 12 Aug 2019 17:18:37: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (142 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。