Job ID = 2589225 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 1,817,076 reads read : 1,817,076 reads written : 1,817,076 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR164255.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:16 1817076 reads; of these: 1817076 (100.00%) were unpaired; of these: 1023459 (56.32%) aligned 0 times 707427 (38.93%) aligned exactly 1 time 86190 (4.74%) aligned >1 times 43.68% overall alignment rate Time searching: 00:00:16 Overall time: 00:00:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 46840 / 793617 = 0.0590 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:10:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:10:58: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:10:58: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:10:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:10:59: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:10:59: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:11:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:11:00: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:11:00: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:11:04: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:11:04: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:11:04: #1 total tags in treatment: 746777 INFO @ Mon, 12 Aug 2019 17:11:04: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:11:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:11:04: #1 tags after filtering in treatment: 746777 INFO @ Mon, 12 Aug 2019 17:11:04: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:11:04: #1 finished! INFO @ Mon, 12 Aug 2019 17:11:04: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:11:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:11:04: #2 number of paired peaks: 2708 INFO @ Mon, 12 Aug 2019 17:11:04: start model_add_line... INFO @ Mon, 12 Aug 2019 17:11:04: start X-correlation... INFO @ Mon, 12 Aug 2019 17:11:04: end of X-cor INFO @ Mon, 12 Aug 2019 17:11:04: #2 finished! INFO @ Mon, 12 Aug 2019 17:11:04: #2 predicted fragment length is 151 bps INFO @ Mon, 12 Aug 2019 17:11:04: #2 alternative fragment length(s) may be 151 bps INFO @ Mon, 12 Aug 2019 17:11:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.05_model.r INFO @ Mon, 12 Aug 2019 17:11:04: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:11:04: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:11:05: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:11:05: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:11:05: #1 total tags in treatment: 746777 INFO @ Mon, 12 Aug 2019 17:11:05: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:11:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:11:05: #1 tags after filtering in treatment: 746777 INFO @ Mon, 12 Aug 2019 17:11:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:11:05: #1 finished! INFO @ Mon, 12 Aug 2019 17:11:05: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:11:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:11:05: #2 number of paired peaks: 2708 INFO @ Mon, 12 Aug 2019 17:11:05: start model_add_line... INFO @ Mon, 12 Aug 2019 17:11:05: start X-correlation... INFO @ Mon, 12 Aug 2019 17:11:05: end of X-cor INFO @ Mon, 12 Aug 2019 17:11:05: #2 finished! INFO @ Mon, 12 Aug 2019 17:11:05: #2 predicted fragment length is 151 bps INFO @ Mon, 12 Aug 2019 17:11:05: #2 alternative fragment length(s) may be 151 bps INFO @ Mon, 12 Aug 2019 17:11:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.10_model.r INFO @ Mon, 12 Aug 2019 17:11:05: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:11:05: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:11:06: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:11:06: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:11:06: #1 total tags in treatment: 746777 INFO @ Mon, 12 Aug 2019 17:11:06: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:11:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:11:06: #1 tags after filtering in treatment: 746777 INFO @ Mon, 12 Aug 2019 17:11:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:11:06: #1 finished! INFO @ Mon, 12 Aug 2019 17:11:06: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:11:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:11:06: #2 number of paired peaks: 2708 INFO @ Mon, 12 Aug 2019 17:11:06: start model_add_line... INFO @ Mon, 12 Aug 2019 17:11:06: start X-correlation... INFO @ Mon, 12 Aug 2019 17:11:06: end of X-cor INFO @ Mon, 12 Aug 2019 17:11:06: #2 finished! INFO @ Mon, 12 Aug 2019 17:11:06: #2 predicted fragment length is 151 bps INFO @ Mon, 12 Aug 2019 17:11:06: #2 alternative fragment length(s) may be 151 bps INFO @ Mon, 12 Aug 2019 17:11:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.20_model.r INFO @ Mon, 12 Aug 2019 17:11:06: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:11:06: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:11:07: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:11:08: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:11:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:11:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:11:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.05_summits.bed INFO @ Mon, 12 Aug 2019 17:11:08: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (2091 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:11:08: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:11:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:11:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:11:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.10_summits.bed INFO @ Mon, 12 Aug 2019 17:11:09: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (764 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:11:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:11:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:11:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX054249/SRX054249.20_summits.bed INFO @ Mon, 12 Aug 2019 17:11:10: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (103 records, 4 fields): 2 millis BedGraph に変換しました。 CompletedMACS2peakCalling BigWig に変換中... BigWig に変換しました。