Job ID = 2589224 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,689,326 reads read : 2,689,326 reads written : 2,689,326 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR164254.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:23 2689326 reads; of these: 2689326 (100.00%) were unpaired; of these: 1152798 (42.87%) aligned 0 times 1408125 (52.36%) aligned exactly 1 time 128403 (4.77%) aligned >1 times 57.13% overall alignment rate Time searching: 00:00:23 Overall time: 00:00:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 256279 / 1536528 = 0.1668 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:11:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:11:20: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:11:20: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:11:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:11:21: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:11:21: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:11:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:11:22: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:11:22: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:11:29: 1000000 INFO @ Mon, 12 Aug 2019 17:11:30: 1000000 INFO @ Mon, 12 Aug 2019 17:11:31: 1000000 INFO @ Mon, 12 Aug 2019 17:11:31: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:11:31: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:11:31: #1 total tags in treatment: 1280249 INFO @ Mon, 12 Aug 2019 17:11:31: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:11:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:11:31: #1 tags after filtering in treatment: 1280249 INFO @ Mon, 12 Aug 2019 17:11:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:11:31: #1 finished! INFO @ Mon, 12 Aug 2019 17:11:31: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:11:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:11:31: #2 number of paired peaks: 3307 INFO @ Mon, 12 Aug 2019 17:11:31: start model_add_line... INFO @ Mon, 12 Aug 2019 17:11:32: start X-correlation... INFO @ Mon, 12 Aug 2019 17:11:32: end of X-cor INFO @ Mon, 12 Aug 2019 17:11:32: #2 finished! INFO @ Mon, 12 Aug 2019 17:11:32: #2 predicted fragment length is 130 bps INFO @ Mon, 12 Aug 2019 17:11:32: #2 alternative fragment length(s) may be 130 bps INFO @ Mon, 12 Aug 2019 17:11:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.05_model.r INFO @ Mon, 12 Aug 2019 17:11:32: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:11:32: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:11:32: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:11:32: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:11:32: #1 total tags in treatment: 1280249 INFO @ Mon, 12 Aug 2019 17:11:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:11:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:11:32: #1 tags after filtering in treatment: 1280249 INFO @ Mon, 12 Aug 2019 17:11:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:11:32: #1 finished! INFO @ Mon, 12 Aug 2019 17:11:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:11:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:11:32: #2 number of paired peaks: 3307 INFO @ Mon, 12 Aug 2019 17:11:32: start model_add_line... INFO @ Mon, 12 Aug 2019 17:11:32: start X-correlation... INFO @ Mon, 12 Aug 2019 17:11:33: end of X-cor INFO @ Mon, 12 Aug 2019 17:11:33: #2 finished! INFO @ Mon, 12 Aug 2019 17:11:33: #2 predicted fragment length is 130 bps INFO @ Mon, 12 Aug 2019 17:11:33: #2 alternative fragment length(s) may be 130 bps INFO @ Mon, 12 Aug 2019 17:11:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.10_model.r INFO @ Mon, 12 Aug 2019 17:11:33: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:11:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:11:33: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:11:33: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:11:33: #1 total tags in treatment: 1280249 INFO @ Mon, 12 Aug 2019 17:11:33: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:11:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:11:33: #1 tags after filtering in treatment: 1280249 INFO @ Mon, 12 Aug 2019 17:11:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:11:33: #1 finished! INFO @ Mon, 12 Aug 2019 17:11:33: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:11:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:11:33: #2 number of paired peaks: 3307 INFO @ Mon, 12 Aug 2019 17:11:33: start model_add_line... INFO @ Mon, 12 Aug 2019 17:11:33: start X-correlation... INFO @ Mon, 12 Aug 2019 17:11:33: end of X-cor INFO @ Mon, 12 Aug 2019 17:11:33: #2 finished! INFO @ Mon, 12 Aug 2019 17:11:33: #2 predicted fragment length is 130 bps INFO @ Mon, 12 Aug 2019 17:11:33: #2 alternative fragment length(s) may be 130 bps INFO @ Mon, 12 Aug 2019 17:11:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.20_model.r INFO @ Mon, 12 Aug 2019 17:11:33: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:11:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:11:36: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:11:37: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:11:38: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:11:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:11:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:11:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.05_summits.bed INFO @ Mon, 12 Aug 2019 17:11:38: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (4916 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:11:39: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:11:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:11:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.10_summits.bed INFO @ Mon, 12 Aug 2019 17:11:39: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (2507 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:11:40: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:11:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:11:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX054248/SRX054248.20_summits.bed INFO @ Mon, 12 Aug 2019 17:11:40: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (681 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。