Job ID = 2589208 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,416,551 reads read : 4,416,551 reads written : 4,416,551 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107596.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:40 4416551 reads; of these: 4416551 (100.00%) were unpaired; of these: 1351905 (30.61%) aligned 0 times 2652436 (60.06%) aligned exactly 1 time 412210 (9.33%) aligned >1 times 69.39% overall alignment rate Time searching: 00:00:40 Overall time: 00:00:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 816651 / 3064646 = 0.2665 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:09:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:09:30: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:09:30: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:09:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:09:31: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:09:31: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:09:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:09:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:09:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:09:37: 1000000 INFO @ Mon, 12 Aug 2019 17:09:38: 1000000 INFO @ Mon, 12 Aug 2019 17:09:38: 1000000 INFO @ Mon, 12 Aug 2019 17:09:45: 2000000 INFO @ Mon, 12 Aug 2019 17:09:45: 2000000 INFO @ Mon, 12 Aug 2019 17:09:46: 2000000 INFO @ Mon, 12 Aug 2019 17:09:47: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:09:47: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:09:47: #1 total tags in treatment: 2247995 INFO @ Mon, 12 Aug 2019 17:09:47: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:09:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:09:47: #1 tags after filtering in treatment: 2247995 INFO @ Mon, 12 Aug 2019 17:09:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:09:47: #1 finished! INFO @ Mon, 12 Aug 2019 17:09:47: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:09:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:09:47: #2 number of paired peaks: 1424 INFO @ Mon, 12 Aug 2019 17:09:47: start model_add_line... INFO @ Mon, 12 Aug 2019 17:09:47: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:09:47: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:09:47: #1 total tags in treatment: 2247995 INFO @ Mon, 12 Aug 2019 17:09:47: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:09:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:09:47: start X-correlation... INFO @ Mon, 12 Aug 2019 17:09:47: end of X-cor INFO @ Mon, 12 Aug 2019 17:09:47: #2 finished! INFO @ Mon, 12 Aug 2019 17:09:47: #2 predicted fragment length is 124 bps INFO @ Mon, 12 Aug 2019 17:09:47: #2 alternative fragment length(s) may be 124 bps INFO @ Mon, 12 Aug 2019 17:09:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.20_model.r INFO @ Mon, 12 Aug 2019 17:09:47: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:09:47: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:09:47: #1 tags after filtering in treatment: 2247995 INFO @ Mon, 12 Aug 2019 17:09:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:09:47: #1 finished! INFO @ Mon, 12 Aug 2019 17:09:47: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:09:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:09:47: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:09:47: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:09:47: #1 total tags in treatment: 2247995 INFO @ Mon, 12 Aug 2019 17:09:47: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:09:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:09:47: #1 tags after filtering in treatment: 2247995 INFO @ Mon, 12 Aug 2019 17:09:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:09:47: #1 finished! INFO @ Mon, 12 Aug 2019 17:09:47: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:09:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:09:48: #2 number of paired peaks: 1424 INFO @ Mon, 12 Aug 2019 17:09:48: start model_add_line... INFO @ Mon, 12 Aug 2019 17:09:48: start X-correlation... INFO @ Mon, 12 Aug 2019 17:09:48: end of X-cor INFO @ Mon, 12 Aug 2019 17:09:48: #2 finished! INFO @ Mon, 12 Aug 2019 17:09:48: #2 predicted fragment length is 124 bps INFO @ Mon, 12 Aug 2019 17:09:48: #2 alternative fragment length(s) may be 124 bps INFO @ Mon, 12 Aug 2019 17:09:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.05_model.r INFO @ Mon, 12 Aug 2019 17:09:48: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:09:48: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:09:48: #2 number of paired peaks: 1424 INFO @ Mon, 12 Aug 2019 17:09:48: start model_add_line... INFO @ Mon, 12 Aug 2019 17:09:48: start X-correlation... INFO @ Mon, 12 Aug 2019 17:09:48: end of X-cor INFO @ Mon, 12 Aug 2019 17:09:48: #2 finished! INFO @ Mon, 12 Aug 2019 17:09:48: #2 predicted fragment length is 124 bps INFO @ Mon, 12 Aug 2019 17:09:48: #2 alternative fragment length(s) may be 124 bps INFO @ Mon, 12 Aug 2019 17:09:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.10_model.r INFO @ Mon, 12 Aug 2019 17:09:48: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:09:48: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:09:54: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:09:55: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:09:55: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.20_summits.bed INFO @ Mon, 12 Aug 2019 17:09:58: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (543 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.05_summits.bed INFO @ Mon, 12 Aug 2019 17:09:58: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (3261 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:09:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX044014/SRX044014.10_summits.bed INFO @ Mon, 12 Aug 2019 17:09:58: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (1748 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。