Job ID = 2589120 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,514,796 reads read : 3,514,796 reads written : 3,514,796 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107306.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:40 3514796 reads; of these: 3514796 (100.00%) were unpaired; of these: 995925 (28.34%) aligned 0 times 2211947 (62.93%) aligned exactly 1 time 306924 (8.73%) aligned >1 times 71.66% overall alignment rate Time searching: 00:00:40 Overall time: 00:00:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 183456 / 2518871 = 0.0728 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 16:57:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 16:57:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 16:57:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 16:57:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 16:57:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 16:57:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 16:57:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 16:57:33: #1 read tag files... INFO @ Mon, 12 Aug 2019 16:57:33: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 16:57:41: 1000000 INFO @ Mon, 12 Aug 2019 16:57:42: 1000000 INFO @ Mon, 12 Aug 2019 16:57:44: 1000000 INFO @ Mon, 12 Aug 2019 16:57:51: 2000000 INFO @ Mon, 12 Aug 2019 16:57:51: 2000000 INFO @ Mon, 12 Aug 2019 16:57:53: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 16:57:53: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 16:57:53: #1 total tags in treatment: 2335415 INFO @ Mon, 12 Aug 2019 16:57:53: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 16:57:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 16:57:54: #1 tags after filtering in treatment: 2335415 INFO @ Mon, 12 Aug 2019 16:57:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 16:57:54: #1 finished! INFO @ Mon, 12 Aug 2019 16:57:54: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 16:57:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 16:57:54: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 16:57:54: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 16:57:54: #1 total tags in treatment: 2335415 INFO @ Mon, 12 Aug 2019 16:57:54: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 16:57:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 16:57:54: #1 tags after filtering in treatment: 2335415 INFO @ Mon, 12 Aug 2019 16:57:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 16:57:54: #1 finished! INFO @ Mon, 12 Aug 2019 16:57:54: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 16:57:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 16:57:54: #2 number of paired peaks: 328 WARNING @ Mon, 12 Aug 2019 16:57:54: Fewer paired peaks (328) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 328 pairs to build model! INFO @ Mon, 12 Aug 2019 16:57:54: start model_add_line... INFO @ Mon, 12 Aug 2019 16:57:54: start X-correlation... INFO @ Mon, 12 Aug 2019 16:57:54: end of X-cor INFO @ Mon, 12 Aug 2019 16:57:54: #2 finished! INFO @ Mon, 12 Aug 2019 16:57:54: #2 predicted fragment length is 93 bps INFO @ Mon, 12 Aug 2019 16:57:54: #2 alternative fragment length(s) may be 93,574 bps INFO @ Mon, 12 Aug 2019 16:57:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.05_model.r INFO @ Mon, 12 Aug 2019 16:57:54: #3 Call peaks... INFO @ Mon, 12 Aug 2019 16:57:54: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 16:57:54: #2 number of paired peaks: 328 WARNING @ Mon, 12 Aug 2019 16:57:54: Fewer paired peaks (328) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 328 pairs to build model! INFO @ Mon, 12 Aug 2019 16:57:54: start model_add_line... INFO @ Mon, 12 Aug 2019 16:57:54: start X-correlation... INFO @ Mon, 12 Aug 2019 16:57:54: end of X-cor INFO @ Mon, 12 Aug 2019 16:57:54: #2 finished! INFO @ Mon, 12 Aug 2019 16:57:54: #2 predicted fragment length is 93 bps INFO @ Mon, 12 Aug 2019 16:57:54: #2 alternative fragment length(s) may be 93,574 bps INFO @ Mon, 12 Aug 2019 16:57:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.10_model.r INFO @ Mon, 12 Aug 2019 16:57:54: #3 Call peaks... INFO @ Mon, 12 Aug 2019 16:57:54: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 16:57:54: 2000000 INFO @ Mon, 12 Aug 2019 16:57:57: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 16:57:57: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 16:57:57: #1 total tags in treatment: 2335415 INFO @ Mon, 12 Aug 2019 16:57:57: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 16:57:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 16:57:58: #1 tags after filtering in treatment: 2335415 INFO @ Mon, 12 Aug 2019 16:57:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 16:57:58: #1 finished! INFO @ Mon, 12 Aug 2019 16:57:58: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 16:57:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 16:57:58: #2 number of paired peaks: 328 WARNING @ Mon, 12 Aug 2019 16:57:58: Fewer paired peaks (328) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 328 pairs to build model! INFO @ Mon, 12 Aug 2019 16:57:58: start model_add_line... INFO @ Mon, 12 Aug 2019 16:57:58: start X-correlation... INFO @ Mon, 12 Aug 2019 16:57:58: end of X-cor INFO @ Mon, 12 Aug 2019 16:57:58: #2 finished! INFO @ Mon, 12 Aug 2019 16:57:58: #2 predicted fragment length is 93 bps INFO @ Mon, 12 Aug 2019 16:57:58: #2 alternative fragment length(s) may be 93,574 bps INFO @ Mon, 12 Aug 2019 16:57:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.20_model.r INFO @ Mon, 12 Aug 2019 16:57:58: #3 Call peaks... INFO @ Mon, 12 Aug 2019 16:57:58: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 16:58:01: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 16:58:01: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 16:58:04: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.05_peaks.xls INFO @ Mon, 12 Aug 2019 16:58:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 16:58:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.05_summits.bed INFO @ Mon, 12 Aug 2019 16:58:04: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (1051 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 16:58:05: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.10_peaks.xls INFO @ Mon, 12 Aug 2019 16:58:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 16:58:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.10_summits.bed INFO @ Mon, 12 Aug 2019 16:58:05: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (208 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 16:58:05: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 16:58:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.20_peaks.xls INFO @ Mon, 12 Aug 2019 16:58:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 16:58:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043849/SRX043849.20_summits.bed INFO @ Mon, 12 Aug 2019 16:58:08: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (15 records, 4 fields): 8 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。