﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="20.1" software_version="Skyline (64-bit) 20.1.0.31 (f09d9bed5)">
  <settings_summary name="デフォルト">
    <peptide_settings>
      <enzyme name="Trypsin" cut="KR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="0" />
      <peptide_prediction use_measured_rts="true" measured_rt_window="2" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0" />
      <peptide_filter start="0" min_length="6" max_length="25" auto_select="true">
        <peptide_exclusions>
          <exclusion name="Met" regex="[M]" />
        </peptide_exclusions>
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1">
        <static_modifications>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" variable="true" formula="H3C2NO" />
        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
    <transition_settings>
      <transition_prediction precursor_mass_type="Monoisotopic" fragment_mass_type="Monoisotopic" optimize_by="None">
        <predict_collision_energy name="Shimadzu QQQ" step_size="1" step_count="5">
          <regression_ce charge="2" slope="0.04" intercept="-0.5082" />
          <regression_ce charge="3" slope="0.037" intercept="-0.8368" />
        </predict_collision_energy>
      </transition_prediction>
      <transition_filter precursor_charges="2,3" product_charges="1,2" precursor_adducts="[M-3H],[M-2H],[M-H],[M-],[M+H],[M+],[M+2H],[M+3H]" product_adducts="[M-3],[M-2],[M-],[M+],[M+2],[M+3]" fragment_types="y,b" small_molecule_fragment_types="f" fragment_range_first="ion 2" fragment_range_last="last ion" precursor_mz_window="0" auto_select="true">
        <measured_ion name="N-terminal to Proline" cut="P" sense="N" min_length="3" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
      <transition_integration />
      <transition_instrument min_mz="50" max_mz="1500" mz_match_tolerance="0.055" />
    </transition_settings>
    <data_settings document_guid="e099346a-8a3c-496e-afac-6c3b7bd52382" />
    <measured_results time_normal_area="true">
      <replicate name="rpsM_12C">
        <sample_file id="rpsM_12C_f0" file_path="C:\Users\81809\Dropbox\共有：プロトコル MS 8060（law data &amp; methods)\Mori\190215\26_tpsI, sJ, sM, rpmG.lcd" sample_name="26_tpsI, sJ, sM, rpmG" modified_time="2019-02-16T07:12:07">
          <instrument_info_list>
            <instrument_info>
              <model>Shimadzu instrument model</model>
              <ionsource>electrospray ionization</ionsource>
              <analyzer>quadrupole/quadrupole/quadrupole</analyzer>
              <detector>conversion dynode electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
    </measured_results>
  </settings_summary>
  <protein name="sp|P0A7S9|RS13_ECOLI" description="30S ribosomal protein S13 OS=Escherichia coli (strain K12) GN=rpsM PE=1 SV=2" accession="P0A7S9" gene="rpsM" species="Escherichia coli (strain K12)" preferred_name="RS13_ECOLI" websearch_status="X">
    <sequence>
        ARIAGINIPD HKHAVIALTS IYGVGKTRSK AILAAAGIAE DVKISELSEG
        QIDTLRDEVA KFVVEGDLRR EISMSIKRLM DLGCYRGLRH RRGLPVRGQR
        TKTNARTRKG PRKPIKK</sequence>
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      <peptide_results>
        <peptide_result replicate="rpsM_12C" peak_count_ratio="0.78125" retention_time="25.396534" />
      </peptide_results>
      <precursor charge="2" calc_neutral_mass="1076.597844" precursor_mz="539.306198" collision_energy="21.064048" modified_sequence="IAGINIPDHK">
        <precursor_results>
          <precursor_peak replicate="rpsM_12C" peak_count_ratio="0.84375" retention_time="25.396534" start_time="24.6861324" end_time="26.2845325" fwhm="1.07416594" area="2.909294E+07" background="9743378" height="186009" truncated="0" identified="false" user_set="FALSE" />
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            <transition_peak replicate="rpsM_12C" retention_time="25.7517338" start_time="24.6861324" end_time="26.2845325" area="297051.625" background="290216.531" height="9201.027" fwhm="0.4928237" truncated="false" identified="false" rank="21" points_across="9" user_set="FALSE" />
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