﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="20.1" software_version="Skyline (64-bit) 20.1.0.31 (f09d9bed5)">
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      <digest_settings max_missed_cleavages="0" />
      <peptide_prediction use_measured_rts="true" measured_rt_window="2" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0" />
      <peptide_filter start="0" min_length="6" max_length="25" auto_select="true">
        <peptide_exclusions>
          <exclusion name="Met" regex="[M]" />
        </peptide_exclusions>
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1">
        <static_modifications>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" variable="true" formula="H3C2NO" />
        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
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      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
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    <data_settings document_guid="54c3f9ea-0f65-4e7c-a159-0bf638ffd09c" />
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            <instrument_info>
              <model>Shimadzu instrument model</model>
              <ionsource>electrospray ionization</ionsource>
              <analyzer>quadrupole/quadrupole/quadrupole</analyzer>
              <detector>conversion dynode electron multiplier</detector>
            </instrument_info>
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        </sample_file>
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        AENQYYGTGR RKSSAARVFI KPGNGKIVIN QRSLEQYFGR ETARMVVRQP
        LELVDMVEKL DLYITVKGGG ISGQAGAIRH GITRALMEYD ESLRSELRKA
        GFVTRDARQV ERKKVGLRKA RRRPQFSKR</sequence>
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        <transition fragment_type="b" fragment_ordinal="3" calc_neutral_mass="381.212452" product_charge="2" cleavage_aa="Q" loss_neutral_mass="0">
          <precursor_mz>254.813417</precursor_mz>
          <product_mz>191.613502</product_mz>
          <collision_energy>8.591296</collision_energy>
          <transition_results>
            <transition_peak replicate="rpsI_12C" retention_time="45.1992" start_time="42.0024" end_time="45.3768" area="41494.5625" background="0" height="1000" fwhm="0.5030227" fwhm_degenerate="true" truncated="false" identified="false" rank="4" points_across="19" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="4" calc_neutral_mass="528.280866" product_charge="2" cleavage_aa="F" loss_neutral_mass="0">
          <precursor_mz>254.813417</precursor_mz>
          <product_mz>265.147709</product_mz>
          <collision_energy>8.591296</collision_energy>
          <transition_results>
            <transition_peak replicate="rpsI_12C" retention_time="42.0024" start_time="42.0024" end_time="45.3768" area="0" background="0" height="0" fwhm="3.37441635" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="19" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="615.312895" product_charge="2" cleavage_aa="S" loss_neutral_mass="0">
          <precursor_mz>254.813417</precursor_mz>
          <product_mz>308.663723</product_mz>
          <collision_energy>8.591296</collision_energy>
          <transition_results>
            <transition_peak replicate="rpsI_12C" retention_time="42.0024" start_time="42.0024" end_time="45.3768" area="0" background="0" height="0" fwhm="3.37441635" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="19" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
  </protein>
</srm_settings>