﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="4.2" software_version="Skyline (64-bit) 4.2.0.19009">
  <settings_summary name="デフォルト">
    <peptide_settings>
      <enzyme name="LysC" cut="K" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="0" />
      <peptide_prediction use_measured_rts="true" measured_rt_window="2" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0" />
      <peptide_filter start="0" min_length="6" max_length="25" auto_select="true">
        <peptide_exclusions>
          <exclusion name="Met" regex="[M]" />
        </peptide_exclusions>
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1">
        <static_modifications>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" formula="H3C2NO" unimod_id="4" short_name="CAM" />
        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
    <transition_settings>
      <transition_prediction precursor_mass_type="Monoisotopic" fragment_mass_type="Monoisotopic" optimize_by="None">
        <predict_collision_energy name="Shimadzu QQQ" step_size="1" step_count="5">
          <regression_ce charge="2" slope="0.04" intercept="-0.5082" />
          <regression_ce charge="3" slope="0.037" intercept="-0.8368" />
        </predict_collision_energy>
      </transition_prediction>
      <transition_filter precursor_charges="2,3" product_charges="1,2" precursor_adducts="[M-3H],[M-2H],[M-H],[M-],[M+H],[M+],[M+2H],[M+3H]" product_adducts="[M-3],[M-2],[M-],[M+],[M+2],[M+3]" fragment_types="b,y" small_molecule_fragment_types="f" fragment_range_first="ion 2" fragment_range_last="last ion" precursor_mz_window="0" auto_select="true">
        <measured_ion name="N-terminal to Proline" cut="P" sense="N" min_length="3" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
      <transition_integration />
      <transition_instrument min_mz="50" max_mz="1500" mz_match_tolerance="0.055" />
    </transition_settings>
    <data_settings document_guid="b2ebe72a-e43a-49af-8c16-0510357dd317" />
    <measured_results time_normal_area="true">
      <replicate name="31_rplW, rpmI lysC">
        <sample_file id="_31_rplW._rpmI_lysC_f0" file_path="C:\Users\81809\Dropbox\共有：プロトコル MS 8060（law data &amp; methods)\Mori\190215\31_rplW, rpmI lysC.lcd" sample_name="31_rplW, rpmI lysC" modified_time="2019-02-16T12:02:24">
          <instrument_info_list>
            <instrument_info>
              <model>Shimadzu instrument model</model>
              <ionsource>electrospray ionization</ionsource>
              <analyzer>quadrupole/quadrupole/quadrupole</analyzer>
              <detector>conversion dynode electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
    </measured_results>
  </settings_summary>
  <protein name="sp|P0A7Q1|RL35_ECOLI" description="50S ribosomal protein L35 OS=Escherichia coli (strain K12) GN=rpmI PE=1 SV=2" accession="P0A7Q1" gene="rpmI" species="Escherichia coli (strain K12)" preferred_name="RL35_ECOLI" websearch_status="X" auto_manage_children="false">
    <sequence>
        PKIKTVRGAA KRFKKTGKGG FKHKHANLRH ILTKKATKRK RHLRPKAMVS
        KGDLGLVIAC LPYA</sequence>
    <peptide sequence="TVRGAAK" modified_sequence="TVRGAAK" start="4" end="11" prev_aa="K" next_aa="R" calc_neutral_pep_mass="701.418423" num_missed_cleavages="0" avg_measured_retention_time="23.75045">
      <peptide_results>
        <peptide_result replicate="31_rplW, rpmI lysC" peak_count_ratio="0.25" retention_time="23.75045" />
      </peptide_results>
      <precursor charge="2" calc_neutral_mass="701.418423" precursor_mz="351.716487" collision_energy="13.560459" modified_sequence="TVRGAAK">
        <precursor_results>
          <precursor_peak replicate="31_rplW, rpmI lysC" peak_count_ratio="0.5" retention_time="23.7863" start_time="23.5712" end_time="23.9297" fwhm="0.226190463" area="1273734.25" background="503525.719" height="98647" truncated="0" identified="false" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="b" fragment_ordinal="2" calc_neutral_mass="200.116092" product_charge="1" cleavage_aa="V" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>201.123368</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="6524.62451" background="56913.9023" height="1462.8894" fwhm="0.07430932" fwhm_degenerate="true" truncated="false" identified="false" rank="17" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="3" calc_neutral_mass="356.217204" product_charge="1" cleavage_aa="R" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>357.22448</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.858" start_time="23.5712" end_time="23.9297" area="11012.2012" background="6959.09473" height="1522.056" fwhm="0.0985736251" fwhm_degenerate="true" truncated="false" identified="false" rank="11" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="4" calc_neutral_mass="413.238667" product_charge="1" cleavage_aa="G" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>414.245943</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.858" start_time="23.5712" end_time="23.9297" area="17277.0781" background="0" height="1138.29138" fwhm="0.298289746" fwhm_degenerate="true" truncated="false" identified="false" rank="9" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="484.275781" product_charge="1" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>485.283057</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.6429" start_time="23.5712" end_time="23.9297" area="10065.5283" background="7476.683" height="1230.9447" fwhm="0.1238226" fwhm_degenerate="true" truncated="false" identified="false" rank="13" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="6" calc_neutral_mass="555.312895" product_charge="1" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>556.320171</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="1684.94031" background="23496.1465" height="0" fwhm="0" fwhm_degenerate="true" truncated="false" identified="false" rank="19" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="2" calc_neutral_mass="200.116092" product_charge="2" cleavage_aa="V" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>101.065322</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.6429" start_time="23.5712" end_time="23.9297" area="22123.2832" background="86041.26" height="2001" fwhm="0.185768917" fwhm_degenerate="true" truncated="false" identified="false" rank="7" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="3" calc_neutral_mass="356.217204" product_charge="2" cleavage_aa="R" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>179.115878</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.858" start_time="23.5712" end_time="23.9297" area="7674.578" background="17208.252" height="1014.87415" fwhm="0.107551575" fwhm_degenerate="true" truncated="false" identified="false" rank="16" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="4" calc_neutral_mass="413.238667" product_charge="2" cleavage_aa="G" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>207.62661</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="727.2865" background="0" height="0" fwhm="0.358505249" fwhm_degenerate="true" truncated="false" identified="false" rank="20" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="484.275781" product_charge="2" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>243.145167</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.858" start_time="23.5712" end_time="23.9297" area="31786.8984" background="34311.5156" height="2326.24658" fwhm="0.227256089" fwhm_degenerate="true" truncated="false" identified="false" rank="6" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="6" calc_neutral_mass="555.312895" product_charge="2" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>278.663723</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.858" start_time="23.5712" end_time="23.9297" area="8394.52051" background="19867.5781" height="1014.33167" fwhm="0.130332321" truncated="false" identified="false" rank="14" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="6" calc_neutral_mass="600.370744" product_charge="1" cleavage_aa="V" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>601.37802</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="11445.71" background="0" height="1074.539" fwhm="0.09951234" fwhm_degenerate="true" truncated="false" identified="false" rank="10" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="5" calc_neutral_mass="501.30233" product_charge="1" cleavage_aa="R" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>502.309606</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.7863" start_time="23.5712" end_time="23.9297" area="46270.0156" background="3993.78418" height="3015.33179" fwhm="0.2789816" fwhm_degenerate="true" truncated="false" identified="false" rank="4" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="4" calc_neutral_mass="345.201219" product_charge="1" cleavage_aa="G" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>346.208495</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.7863" start_time="23.5712" end_time="23.9297" area="872421.25" background="111875.148" height="98647" fwhm="0.145572737" truncated="false" identified="false" rank="1" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="3" calc_neutral_mass="288.179755" product_charge="1" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>289.187031</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.7146" start_time="23.5712" end_time="23.9297" area="80379.26" background="75153.48" height="6709.166" fwhm="0.207873523" truncated="false" identified="false" rank="2" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="2" calc_neutral_mass="217.142642" product_charge="1" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>218.149918</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.7146" start_time="23.5712" end_time="23.9297" area="10990.4609" background="0" height="1000" fwhm="0.154038653" truncated="false" identified="false" rank="12" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="6" calc_neutral_mass="600.370744" product_charge="2" cleavage_aa="V" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>301.192648</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.7146" start_time="23.5712" end_time="23.9297" area="17943.93" background="21510.3145" height="1600" fwhm="0.1688677" truncated="false" identified="false" rank="8" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="5" calc_neutral_mass="501.30233" product_charge="2" cleavage_aa="R" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>251.658441</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.7863" start_time="23.5712" end_time="23.9297" area="8274.88" background="8604.126" height="600" fwhm="0.244461462" fwhm_degenerate="true" truncated="false" identified="false" rank="15" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="4" calc_neutral_mass="345.201219" product_charge="2" cleavage_aa="G" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>173.607886</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="4566.714" background="0" height="400" fwhm="0.185768917" fwhm_degenerate="true" truncated="false" identified="false" rank="18" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="3" calc_neutral_mass="288.179755" product_charge="2" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>145.097154</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.7863" start_time="23.5712" end_time="23.9297" area="34858.1055" background="17208.252" height="2600" fwhm="0.226190463" truncated="false" identified="false" rank="5" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="2" calc_neutral_mass="217.142642" product_charge="2" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>351.716487</precursor_mz>
          <product_mz>109.578597</product_mz>
          <collision_energy>13.560459</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.7863" start_time="23.5712" end_time="23.9297" area="69312.91" background="12906.1895" height="7002" fwhm="0.157557189" truncated="false" identified="false" rank="3" points_across="5" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
      <precursor charge="3" calc_neutral_mass="701.418423" precursor_mz="234.813417" collision_energy="7.851296" modified_sequence="TVRGAAK">
        <precursor_results>
          <precursor_peak replicate="31_rplW, rpmI lysC" peak_count_ratio="0" retention_time="23.7146" start_time="23.5712" end_time="23.9297" fwhm="0.21035926" area="89081.92" background="772635.563" height="4002" truncated="0" identified="false" user_set="FALSE" />
        </precursor_results>
        <transition fragment_type="b" fragment_ordinal="2" calc_neutral_mass="200.116092" product_charge="1" cleavage_aa="V" loss_neutral_mass="0">
          <precursor_mz>234.813417</precursor_mz>
          <product_mz>201.123368</product_mz>
          <collision_energy>7.851296</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="0" background="0" height="0" fwhm="0.358505249" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="3" calc_neutral_mass="356.217204" product_charge="1" cleavage_aa="R" loss_neutral_mass="0">
          <precursor_mz>234.813417</precursor_mz>
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          <collision_energy>7.851296</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="0" background="0" height="0" fwhm="0.358505249" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="4" calc_neutral_mass="413.238667" product_charge="1" cleavage_aa="G" loss_neutral_mass="0">
          <precursor_mz>234.813417</precursor_mz>
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          <collision_energy>7.851296</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="0" background="0" height="0" fwhm="0.358505249" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="484.275781" product_charge="1" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>234.813417</precursor_mz>
          <product_mz>485.283057</product_mz>
          <collision_energy>7.851296</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="0" background="0" height="0" fwhm="0.358505249" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="6" calc_neutral_mass="555.312895" product_charge="1" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>234.813417</precursor_mz>
          <product_mz>556.320171</product_mz>
          <collision_energy>7.851296</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.5712" start_time="23.5712" end_time="23.9297" area="0" background="0" height="0" fwhm="0.358505249" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="2" calc_neutral_mass="200.116092" product_charge="2" cleavage_aa="V" loss_neutral_mass="0">
          <precursor_mz>234.813417</precursor_mz>
          <product_mz>101.065322</product_mz>
          <collision_energy>7.851296</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.6429" start_time="23.5712" end_time="23.9297" area="6630.89063" background="348596.156" height="601" fwhm="0.187714174" fwhm_degenerate="true" truncated="false" identified="false" rank="3" points_across="5" user_set="FALSE" forced_integration="true" />
          </transition_results>
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        </transition>
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        </transition>
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          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="659.397962" product_charge="2" cleavage_aa="P" loss_neutral_mass="0">
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            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="24.445467" start_time="23.728466" end_time="24.5171661" area="26699.8047" background="8745.594" height="2611.24072" fwhm="0.1496208" fwhm_degenerate="true" truncated="false" identified="false" rank="1" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="5" calc_neutral_mass="649.402379" product_charge="1" cleavage_aa="H" loss_neutral_mass="0">
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            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.728466" start_time="23.728466" end_time="24.5171661" area="0" background="0" height="0" fwhm="0.788711548" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="4" calc_neutral_mass="512.343467" product_charge="1" cleavage_aa="L" loss_neutral_mass="0">
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          <collision_energy>9.135012</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.728466" start_time="23.728466" end_time="24.5171661" area="0" background="0" height="0" fwhm="0.788711548" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="3" calc_neutral_mass="399.259403" product_charge="1" cleavage_aa="R" loss_neutral_mass="0">
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          <collision_energy>9.135012</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.728466" start_time="23.728466" end_time="24.5171661" area="0" background="0" height="0" fwhm="0.788711548" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="2" calc_neutral_mass="243.158292" product_charge="1" cleavage_aa="P" loss_neutral_mass="0">
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          <collision_energy>9.135012</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="24.1586666" start_time="23.728466" end_time="24.5171661" area="860.8057" background="0" height="162.822723" fwhm="0.0823419839" truncated="false" identified="false" rank="4" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="5" calc_neutral_mass="649.402379" product_charge="2" cleavage_aa="H" loss_neutral_mass="0">
          <precursor_mz>269.508439</precursor_mz>
          <product_mz>325.708465</product_mz>
          <collision_energy>9.135012</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.728466" start_time="23.728466" end_time="24.5171661" area="0" background="0" height="0" fwhm="0.788711548" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="4" calc_neutral_mass="512.343467" product_charge="2" cleavage_aa="L" loss_neutral_mass="0">
          <precursor_mz>269.508439</precursor_mz>
          <product_mz>257.179009</product_mz>
          <collision_energy>9.135012</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="23.728466" start_time="23.728466" end_time="24.5171661" area="0" background="0" height="0" fwhm="0.788711548" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="3" calc_neutral_mass="399.259403" product_charge="2" cleavage_aa="R" loss_neutral_mass="0">
          <precursor_mz>269.508439</precursor_mz>
          <product_mz>200.636977</product_mz>
          <collision_energy>9.135012</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="24.0869675" start_time="23.728466" end_time="24.5171661" area="3443.29785" background="0" height="651.6738" fwhm="0.08230041" truncated="false" identified="false" rank="3" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="y" fragment_ordinal="2" calc_neutral_mass="243.158292" product_charge="2" cleavage_aa="P" loss_neutral_mass="0">
          <precursor_mz>269.508439</precursor_mz>
          <product_mz>122.586422</product_mz>
          <collision_energy>9.135012</collision_energy>
          <transition_results>
            <transition_peak replicate="31_rplW, rpmI lysC" retention_time="24.3737659" start_time="23.728466" end_time="24.5171661" area="5944.7373" background="0" height="600" fwhm="0.165522024" truncated="false" identified="false" rank="2" points_across="11" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
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</srm_settings>