﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="20.1" software_version="Skyline (64-bit) 20.1.0.31 (f09d9bed5)">
  <settings_summary name="デフォルト">
    <peptide_settings>
      <enzyme name="Trypsin" cut="KR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="0" />
      <peptide_prediction use_measured_rts="true" measured_rt_window="2" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0" />
      <peptide_filter start="0" min_length="6" max_length="25" auto_select="true">
        <peptide_exclusions>
          <exclusion name="Met" regex="[M]" />
        </peptide_exclusions>
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1">
        <static_modifications>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" formula="H3C2NO" unimod_id="4" short_name="CAM" />
        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
    <transition_settings>
      <transition_prediction precursor_mass_type="Monoisotopic" fragment_mass_type="Monoisotopic" optimize_by="None">
        <predict_collision_energy name="Shimadzu QQQ" step_size="1" step_count="5">
          <regression_ce charge="2" slope="0.04" intercept="-0.5082" />
          <regression_ce charge="3" slope="0.037" intercept="-0.8368" />
        </predict_collision_energy>
      </transition_prediction>
      <transition_filter precursor_charges="2,3" product_charges="1,2" precursor_adducts="[M-3H],[M-2H],[M-H],[M-],[M+H],[M+],[M+2H],[M+3H]" product_adducts="[M-3],[M-2],[M-],[M+],[M+2],[M+3]" fragment_types="b,y" small_molecule_fragment_types="f" fragment_range_first="ion 2" fragment_range_last="last ion" precursor_mz_window="0" auto_select="true">
        <measured_ion name="N-terminal to Proline" cut="P" sense="N" min_length="3" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
      <transition_integration />
      <transition_instrument min_mz="50" max_mz="1500" mz_match_tolerance="0.055" />
    </transition_settings>
    <data_settings document_guid="419dada3-2f87-43f7-bf9d-986a4eaf931b" />
    <measured_results time_normal_area="true">
      <replicate name="rpmE_12C">
        <sample_file id="rpmE_12C_f0" file_path="C:\Users\81809\Dropbox\共有：プロトコル MS 8060（law data &amp; methods)\Mori\190209\12_rpmE.lcd" sample_name="12_rpmE" modified_time="2019-02-10T05:25:57">
          <instrument_info_list>
            <instrument_info>
              <model>Shimadzu instrument model</model>
              <ionsource>electrospray ionization</ionsource>
              <analyzer>quadrupole/quadrupole/quadrupole</analyzer>
              <detector>conversion dynode electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
    </measured_results>
  </settings_summary>
  <protein name="sp|P0A7M9|RL31_ECOLI" description="50S ribosomal protein L31 OS=Escherichia coli (strain K12) GN=rpmE PE=1 SV=1" accession="P0A7M9" gene="rpmE" species="Escherichia coli (strain K12)" preferred_name="RL31_ECOLI" websearch_status="X">
    <sequence>
        MKKDIHPKYE EITASCSCGN VMKIRSTVGH DLNLDVCSKC HPFFTGKQRD
        VATGGRVDRF NKRFNIPGS</sequence>
    <peptide sequence="STVGHDLNLDVCSK" modified_sequence="STVGHDLNLDVC[+57.021464]SK" start="25" end="39" prev_aa="R" next_aa="C" calc_neutral_pep_mass="1543.730057" num_missed_cleavages="0" avg_measured_retention_time="25.7557">
      <implicit_modifications>
        <implicit_static_modifications>
          <implicit_modification index_aa="11" modification_name="Carbamidomethyl (C)" mass_diff="+57" />
        </implicit_static_modifications>
        <implicit_heavy_modifications />
      </implicit_modifications>
      <peptide_results>
        <peptide_result replicate="rpmE_12C" peak_count_ratio="0.8020833" retention_time="25.7557" />
      </peptide_results>
      <precursor charge="2" calc_neutral_mass="1543.730057" precursor_mz="772.872304" collision_energy="30.406692" modified_sequence="STVGHDLNLDVC[+57.0]SK">
        <precursor_results>
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</srm_settings>