﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="20.1" software_version="Skyline (64-bit) 20.1.0.31 (f09d9bed5)">
  <settings_summary name="デフォルト">
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      <enzyme name="Trypsin" cut="KR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="0" />
      <peptide_prediction use_measured_rts="true" measured_rt_window="2" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0" />
      <peptide_filter start="0" min_length="6" max_length="25" auto_select="true">
        <peptide_exclusions>
          <exclusion name="Met" regex="[M]" />
        </peptide_exclusions>
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1">
        <static_modifications>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" formula="H3C2NO" unimod_id="4" short_name="CAM" />
        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
    <transition_settings>
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        <predict_collision_energy name="Shimadzu QQQ" step_size="1" step_count="5">
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          <regression_ce charge="3" slope="0.037" intercept="-0.8368" />
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      </transition_prediction>
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        <measured_ion name="N-terminal to Proline" cut="P" sense="N" min_length="3" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
      <transition_integration />
      <transition_instrument min_mz="50" max_mz="1500" mz_match_tolerance="0.055" />
    </transition_settings>
    <data_settings document_guid="0b9f865c-973c-4626-aa1c-d6a10863eef1" />
    <measured_results time_normal_area="true">
      <replicate name="rpmD_12C">
        <sample_file id="rpmD_12C_f0" file_path="C:\Users\81809\Dropbox\共有：プロトコル MS 8060（law data &amp; methods)\Mori\190215\20_rplU, lW, lX, lY, mD.lcd" sample_name="20_rplU, lW, lX, lY, mD" modified_time="2019-02-16T01:32:44">
          <instrument_info_list>
            <instrument_info>
              <model>Shimadzu instrument model</model>
              <ionsource>electrospray ionization</ionsource>
              <analyzer>quadrupole/quadrupole/quadrupole</analyzer>
              <detector>conversion dynode electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
    </measured_results>
  </settings_summary>
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    <sequence>
        MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA
        VSFMVKVEE</sequence>
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          <collision_energy>9.072104</collision_energy>
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          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="4" calc_neutral_mass="442.169979" product_charge="1" cleavage_aa="P" loss_neutral_mass="0">
          <precursor_mz>267.80822</precursor_mz>
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            <transition_peak replicate="rpmD_12C" retention_time="25.1528" start_time="25.1528" end_time="26.7728" area="0" background="0" height="0" fwhm="1.62000275" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="9" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="513.207092" product_charge="1" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>267.80822</precursor_mz>
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          <collision_energy>9.072104</collision_energy>
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            <transition_peak replicate="rpmD_12C" retention_time="25.1528" start_time="25.1528" end_time="26.7728" area="0" background="0" height="0" fwhm="1.62000275" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="9" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="6" calc_neutral_mass="626.291156" product_charge="1" cleavage_aa="I" loss_neutral_mass="0">
          <precursor_mz>267.80822</precursor_mz>
          <product_mz>627.298432</product_mz>
          <collision_energy>9.072104</collision_energy>
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            <transition_peak replicate="rpmD_12C" retention_time="25.1528" start_time="25.1528" end_time="26.7728" area="0" background="0" height="0" fwhm="1.62000275" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="9" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="2" calc_neutral_mass="244.069536" product_charge="2" cleavage_aa="D" loss_neutral_mass="0">
          <precursor_mz>267.80822</precursor_mz>
          <product_mz>123.042044</product_mz>
          <collision_energy>9.072104</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmD_12C" retention_time="25.1528" start_time="25.1528" end_time="26.7728" area="55728.125" background="575.958252" height="1191.11169" fwhm="0.271349043" fwhm_degenerate="true" truncated="false" identified="false" rank="2" points_across="9" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="3" calc_neutral_mass="345.117215" product_charge="2" cleavage_aa="T" loss_neutral_mass="0">
          <precursor_mz>267.80822</precursor_mz>
          <product_mz>173.565883</product_mz>
          <collision_energy>9.072104</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmD_12C" retention_time="25.1528" start_time="25.1528" end_time="26.7728" area="0" background="0" height="0" fwhm="1.62000275" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="9" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="4" calc_neutral_mass="442.169979" product_charge="2" cleavage_aa="P" loss_neutral_mass="0">
          <precursor_mz>267.80822</precursor_mz>
          <product_mz>222.092265</product_mz>
          <collision_energy>9.072104</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmD_12C" retention_time="26.5928" start_time="25.1528" end_time="26.7728" area="141557.828" background="26559.998" height="5589.149" fwhm="0.379427224" truncated="false" identified="false" rank="1" points_across="9" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="513.207092" product_charge="2" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>267.80822</precursor_mz>
          <product_mz>257.610822</product_mz>
          <collision_energy>9.072104</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmD_12C" retention_time="25.1528" start_time="25.1528" end_time="26.7728" area="2112.00732" background="31.9976826" height="0" fwhm="0" fwhm_degenerate="true" truncated="false" identified="false" rank="10" points_across="9" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="6" calc_neutral_mass="626.291156" product_charge="2" cleavage_aa="I" loss_neutral_mass="0">
          <precursor_mz>267.80822</precursor_mz>
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          <collision_energy>9.072104</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmD_12C" retention_time="25.1528" start_time="25.1528" end_time="26.7728" area="0" background="0" height="0" fwhm="1.62000275" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="9" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
  </protein>
</srm_settings>