﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="20.1" software_version="Skyline (64-bit) 20.1.0.31 (f09d9bed5)">
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      <peptide_prediction use_measured_rts="true" measured_rt_window="2" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0" />
      <peptide_filter start="0" min_length="6" max_length="25" auto_select="true">
        <peptide_exclusions>
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        </peptide_exclusions>
      </peptide_filter>
      <peptide_libraries pick="library" />
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        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
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      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
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    <data_settings document_guid="076bd071-544e-4afc-bd27-aaeeae785964" />
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          <instrument_info_list>
            <instrument_info>
              <model>Shimadzu instrument model</model>
            </instrument_info>
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        </sample_file>
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  </settings_summary>
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    <sequence>
        SRVCQVTGKR PVTGNNRSHA LNATKRRFLP NLHSHRFWVE SEKRFVTLRV
        SAKGMRVIDK KGIDTVLAEL RARGEKY</sequence>
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      </implicit_modifications>
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        <transition fragment_type="b" fragment_ordinal="8" calc_neutral_mass="798.412334" product_charge="1" cleavage_aa="E" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>799.41961</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="9" calc_neutral_mass="911.496398" product_charge="1" cleavage_aa="L" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>912.503674</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="2" calc_neutral_mass="170.105528" product_charge="2" cleavage_aa="I" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>86.06004</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="3" calc_neutral_mass="285.132471" product_charge="2" cleavage_aa="D" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>143.573511</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="4" calc_neutral_mass="386.180149" product_charge="2" cleavage_aa="T" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>194.097351</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="5" calc_neutral_mass="485.248563" product_charge="2" cleavage_aa="V" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>243.631558</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="6" calc_neutral_mass="598.332627" product_charge="2" cleavage_aa="L" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>300.17359</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="7" calc_neutral_mass="669.369741" product_charge="2" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>335.692147</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="8" calc_neutral_mass="798.412334" product_charge="2" cleavage_aa="E" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>400.213443</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="9" calc_neutral_mass="911.496398" product_charge="2" cleavage_aa="L" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>456.755475</product_mz>
          <collision_energy>12.589635</collision_energy>
          <transition_results>
            <transition_peak replicate="rpmB_12C" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
  </protein>
</srm_settings>