﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="20.1" software_version="Skyline (64-bit) 20.1.0.31 (f09d9bed5)">
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      <enzyme name="Trypsin" cut="KR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="0" />
      <peptide_prediction use_measured_rts="true" measured_rt_window="2" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0" />
      <peptide_filter start="0" min_length="6" max_length="25" auto_select="true">
        <peptide_exclusions>
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        </peptide_exclusions>
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1">
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        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
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      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
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    <data_settings document_guid="7b7a953e-d578-4c22-ab32-e87b290fb704" />
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          <instrument_info_list>
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              <model>Shimadzu instrument model</model>
              <ionsource>electrospray ionization</ionsource>
              <analyzer>quadrupole/quadrupole/quadrupole</analyzer>
              <detector>conversion dynode electron multiplier</detector>
            </instrument_info>
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        </sample_file>
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    <sequence>
        SRVCQVTGKR PVTGNNRSHA LNATKRRFLP NLHSHRFWVE SEKRFVTLRV
        SAKGMRVIDK KGIDTVLAEL RARGEKY</sequence>
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      </implicit_modifications>
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        </transition>
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          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="6" calc_neutral_mass="598.332627" product_charge="2" cleavage_aa="L" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
          <product_mz>300.17359</product_mz>
          <collision_energy>12.589635</collision_energy>
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            <transition_peak replicate="rpmB_12C" retention_time="32.7945328" start_time="32.4531326" end_time="34.5015335" area="184339.359" background="50196.7422" height="3992.578" fwhm="0.6252187" truncated="false" identified="false" rank="1" points_across="12" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="7" calc_neutral_mass="669.369741" product_charge="2" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
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          <collision_energy>12.589635</collision_energy>
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            <transition_peak replicate="rpmB_12C" retention_time="32.6238327" start_time="32.4531326" end_time="34.5015335" area="20443.9473" background="0" height="1992.57922" fwhm="0.171002209" truncated="false" identified="false" rank="6" points_across="12" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="8" calc_neutral_mass="798.412334" product_charge="2" cleavage_aa="E" loss_neutral_mass="0">
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          <collision_energy>12.589635</collision_energy>
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          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="9" calc_neutral_mass="911.496398" product_charge="2" cleavage_aa="L" loss_neutral_mass="0">
          <precursor_mz>362.876634</precursor_mz>
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          <collision_energy>12.589635</collision_energy>
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            <transition_peak replicate="rpmB_12C" retention_time="32.4531326" start_time="32.4531326" end_time="34.5015335" area="0" background="0" height="0" fwhm="2.04840088" fwhm_degenerate="true" truncated="false" identified="false" rank="0" points_across="12" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
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</srm_settings>