﻿<?xml version="1.0" encoding="utf-8"?>
<srm_settings format_version="20.1" software_version="Skyline (64-bit) 20.1.0.31 (f09d9bed5)">
  <settings_summary name="デフォルト">
    <peptide_settings>
      <enzyme name="Trypsin" cut="KR" no_cut="P" sense="C" />
      <digest_settings max_missed_cleavages="0" />
      <peptide_prediction use_measured_rts="true" measured_rt_window="2" use_spectral_library_drift_times="false" spectral_library_drift_times_peak_width_calc_type="resolving_power" spectral_library_drift_times_resolving_power="0" spectral_library_drift_times_width_at_dt_zero="0" spectral_library_drift_times_width_at_dt_max="0" />
      <peptide_filter start="0" min_length="6" max_length="25" auto_select="true">
        <peptide_exclusions>
          <exclusion name="Met" regex="[M]" />
        </peptide_exclusions>
      </peptide_filter>
      <peptide_libraries pick="library" />
      <peptide_modifications max_variable_mods="3" max_neutral_losses="1">
        <static_modifications>
          <static_modification name="Carbamidomethyl (C)" aminoacid="C" formula="H3C2NO" unimod_id="4" short_name="CAM" />
        </static_modifications>
        <heavy_modifications />
      </peptide_modifications>
    </peptide_settings>
    <transition_settings>
      <transition_prediction precursor_mass_type="Monoisotopic" fragment_mass_type="Monoisotopic" optimize_by="None">
        <predict_collision_energy name="Shimadzu QQQ" step_size="1" step_count="5">
          <regression_ce charge="2" slope="0.04" intercept="-0.5082" />
          <regression_ce charge="3" slope="0.037" intercept="-0.8368" />
        </predict_collision_energy>
      </transition_prediction>
      <transition_filter precursor_charges="2,3" product_charges="1,2" precursor_adducts="[M-3H],[M-2H],[M-H],[M-],[M+H],[M+],[M+2H],[M+3H]" product_adducts="[M-3],[M-2],[M-],[M+],[M+2],[M+3]" fragment_types="y,b" small_molecule_fragment_types="f" fragment_range_first="ion 2" fragment_range_last="last ion" precursor_mz_window="0" auto_select="true">
        <measured_ion name="N-terminal to Proline" cut="P" sense="N" min_length="3" />
      </transition_filter>
      <transition_libraries ion_match_tolerance="0.5" min_ion_count="0" ion_count="3" pick_from="all" />
      <transition_integration />
      <transition_instrument min_mz="50" max_mz="1500" mz_match_tolerance="0.055" />
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    <data_settings document_guid="c4f7eb0c-83d7-4f17-9f9e-b37146f303cb" />
    <measured_results time_normal_area="true">
      <replicate name="rplD_12C">
        <sample_file id="rplD_12C_f0" file_path="C:\Users\81809\Dropbox\共有：プロトコル MS 8060（law data &amp; methods)\Mori\190215\13_rplB, lD.lcd" sample_name="13_rplB, lD" modified_time="2019-02-15T18:56:42">
          <instrument_info_list>
            <instrument_info>
              <model>Shimadzu instrument model</model>
              <ionsource>electrospray ionization</ionsource>
              <analyzer>quadrupole/quadrupole/quadrupole</analyzer>
              <detector>conversion dynode electron multiplier</detector>
            </instrument_info>
          </instrument_info_list>
        </sample_file>
      </replicate>
    </measured_results>
  </settings_summary>
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    <sequence>
        MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA
        EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARPQ DHSQKVNKKM
        YRGALKSILS ELVRQDRLIV VEKFSVEAPK TKLLAQKLKD MALEDVLIIT
        GELDENLFLA ARNLHKVDVR DATGIDPVSL IAFDKVVMTA DAVKQVEEML
        A</sequence>
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      <peptide_results>
        <peptide_result replicate="rplD_12C" peak_count_ratio="0.596153855" retention_time="23.5998344" />
      </peptide_results>
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        </transition>
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        </transition>
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        </transition>
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        </transition>
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        </transition>
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          <collision_energy>18.45038</collision_energy>
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            <transition_peak replicate="rplD_12C" retention_time="21.7442" start_time="17.6234" end_time="23.2895" area="1264747.13" background="0" height="10581.3721" fwhm="2.46702385" truncated="false" identified="false" rank="4" points_across="33" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="10" calc_neutral_mass="968.481476" product_charge="2" cleavage_aa="L" loss_neutral_mass="0">
          <precursor_mz>521.275122</precursor_mz>
          <product_mz>485.248014</product_mz>
          <collision_energy>18.45038</collision_energy>
          <transition_results>
            <transition_peak replicate="rplD_12C" retention_time="22.7744" start_time="17.6234" end_time="23.2895" area="924535.4" background="750445.438" height="12250.02" fwhm="1.53309608" truncated="false" identified="false" rank="8" points_across="33" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="11" calc_neutral_mass="1081.56554" product_charge="2" cleavage_aa="I" loss_neutral_mass="0">
          <precursor_mz>521.275122</precursor_mz>
          <product_mz>541.790046</product_mz>
          <collision_energy>18.45038</collision_energy>
          <transition_results>
            <transition_peak replicate="rplD_12C" retention_time="22.9461" start_time="17.6234" end_time="23.2895" area="537876.063" background="0" height="5201" fwhm="1.49123478" truncated="false" identified="false" rank="23" points_across="33" user_set="FALSE" forced_integration="true" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="12" calc_neutral_mass="1152.602654" product_charge="2" cleavage_aa="A" loss_neutral_mass="0">
          <precursor_mz>521.275122</precursor_mz>
          <product_mz>577.308603</product_mz>
          <collision_energy>18.45038</collision_energy>
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            <transition_peak replicate="rplD_12C" retention_time="21.0574" start_time="17.6234" end_time="23.2895" area="624118.2" background="0" height="15208" fwhm="0.3700712" truncated="false" identified="false" rank="16" points_across="33" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="13" calc_neutral_mass="1299.671068" product_charge="2" cleavage_aa="F" loss_neutral_mass="0">
          <precursor_mz>521.275122</precursor_mz>
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          <collision_energy>18.45038</collision_energy>
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            <transition_peak replicate="rplD_12C" retention_time="22.431" start_time="17.6234" end_time="23.2895" area="595520.25" background="0" height="5001" fwhm="3.31972766" fwhm_degenerate="true" truncated="false" identified="false" rank="21" points_across="33" user_set="FALSE" />
          </transition_results>
        </transition>
        <transition fragment_type="b" fragment_ordinal="14" calc_neutral_mass="1414.698011" product_charge="2" cleavage_aa="D" loss_neutral_mass="0">
          <precursor_mz>521.275122</precursor_mz>
          <product_mz>708.356282</product_mz>
          <collision_energy>18.45038</collision_energy>
          <transition_results>
            <transition_peak replicate="rplD_12C" retention_time="23.1178" start_time="17.6234" end_time="23.2895" area="415694.156" background="0" height="4155.768" fwhm="2.28553367" fwhm_degenerate="true" truncated="false" identified="false" rank="28" points_across="33" user_set="FALSE" />
          </transition_results>
        </transition>
      </precursor>
    </peptide>
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</srm_settings>