大項目（日本語） 小項目（日本語） 大項目（英語） 小項目（英語） レーン1 レーン2 レーン3 レーン4 レーン5 レーン6 レーン7 レーン8 レーン9 レーン10 レーン11 レーン12 レーン13 レーン14 レーン15 レーン16 レーン17 レーン18 レーン19 レーン20 参照レーン - reference lane アクセッション番号 アクセッション番号 accession number accession number 000208-1.1* 000208-2.1_ 000208-3.1_ 000211-1.1* 000211-2.1_ 000211-3.1_ 000214-1.1* 000214-2.1_ サンプル登録 採取者名 sample registration collector Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga Yuko Suga 所属名 affiliation National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region National Agricultural Research Center for Western Region 採取年月日 sampling date 4/3/2007 4/3/2007 4/3/2007 4/3/2007 4/3/2007 4/3/2007 4/3/2007 4/3/2007 試験目的 試験目的 experimental plan experimental purpose Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields Effects of long-term fertilization on soil microbial community structure and spinach yields 試験設計 試験設計 experimental plan experimental plan 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions 9plots= 3 treatments(N=10,20,40kg/10a) * 3 repetitions サンプル情報 サンプル情報 sample information sample information N10-1-1 N10-2-1 N10-3-1 N20-1-1 N20-2-1 N20-3-1 N40-1-1 N40-2-1 試験区面積 試験区面積 experimental plot (m^2) experimental plot 3.9 3.9 3.9 3.9 3.9 3.9 3.9 3.9 採取場所 備考 note aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO aono, ayabe, KYOTO 由来 由来 origin origin non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere 備考 note 採取土壌群 土壌群 soil groups soil groups Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils Brown Lowland soils 土地利用 土地利用 land use land use upland upland upland upland upland upland upland upland 土壌物理化学性 土性 physical and chemical properties of soil soil texture SiCL SiCL SiCL SiCL SiCL SiCL SiCL SiCL pH (風乾土) pH (dry soil) 6.75 7.15 6.7 6.25 6.15 6.2 5.15 5.8 EC EC (electric conductivity) 0.1849 0.15555 0.364 0.352 0.467 0.321 2.14 1.112 炭素含有率 Total C 0.75813553012175 0.82450505512145 0.799149365526 0.81176347156461 0.80598483341929 0.85767182684252 0.90403766192378 0.84374799210846 窒素含有率 Total N 0.090790034571178 0.0926333245507 0.099790659479587 0.097551862462443 0.099571736524036 0.099903218923715 0.15158865688732 0.12104064334435 土壌バイオマス soil biomass 20.555580962271 65.69991135521 46.248725618424 74.688400692288 57.028994932041 40.527382428122 27.677245635928 26.272868800535 可給態P available P 453.36094049729 528.50208256826 551.48628166615 789.7825372274 740.12574103392 725.86055195346 1538.9281337206 1859.0782679624 窒素無機化量 nitrogen mineralization rates 土壌呼吸活性 soil respiration activity 土壌水分（生土あたりの含水量） soil moisture content 14.210802307289 14.884696016771 14.064914992272 15.380521554018 15.216258467952 14.655618850337 15.769439912996 15.186104218362 陽イオン交換容量 cation exchange capacity (CEC) その他 the others Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils Fine textured Brown Lowland soils 栽培作物 作物名 crop crop spinach spinach spinach spinach spinach spinach spinach spinach 品種 cultivar kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray kyouryoku ouray 栽植密度 planting density (1) 栽培様式 planting density (2) 25*6 25*6 25*6 25*6 25*6 25*6 25*6 25*6 備考 note 有機質肥料 有機物の種類 organic fertilizer kind of organic material non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer non-fertilizer 施用量 rate of application 連用年数 years of continuous application 施用時期 time of fertilizer application 備考 note 化学肥料 肥料の種類 chemical fertilizer kind of fertilizer Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate Ammonium nitrate, multi-phosphate, Potassium sulfate 施用量(N) rate of fertilizer application(N) 10 10 10 20 20 20 40 40 施用量(P) rate of fertilizer application(P) 40 40 40 80 80 80 200 200 施用量(K) rate of fertilizer application(K) 25 25 25 50 50 50 150 150 連用年数 years of continuous application 10 10 10 10 10 10 10 10 施用時期 time of fertilizer application winter winter winter winter winter winter winter winter 薬剤散布 薬剤の種類 pesticide application kind of pesticide 施用量 rate of pesticide application 連用年数 years of continuous application 施用時期 time of fertilizer application 水管理 水管理 water management water management 試験結果 試験結果 experimental result experimental result Average of the leaf length　26.9 cm, Average of the leaf weight　27.3 g Average of the leaf length　30.8 cm, Average of the leaf weight　26.2 g Average of the leaf length　29.7 cm, Average of the leaf weight　28.4 g Average of the leaf length　29.8 cm, Average of the leaf weight　33.1 g Average of the leaf length　25 cm, Average of the leaf weight　22.6 g Average of the leaf length　30 cm, Average of the leaf weight　28.7 g Average of the leaf length　24.6 cm, Average of the leaf weight　21.9 g Average of the leaf length　20.6 cm, Average of the leaf weight　14.7 g 栽培暦 前作物名 cultivation history former ｃｕｌｔｉｖａｔｅｄ crop spinach spinach spinach spinach spinach spinach spinach spinach 作業内容 seeding or transplanting seeding seeding seeding seeding seeding seeding seeding seeding 作業日 seeding or transplanting date 2/21/2007 2/21/2007 2/21/2007 2/21/2007 2/21/2007 2/21/2007 2/21/2007 2/21/2007 収穫日 harvest date 4/3/2007 4/3/2007 4/3/2007 4/3/2007 4/3/2007 4/3/2007 4/3/2007 4/3/2007 気温 気温 temperature temperature(℃) DNA 抽出法 DNA sequence DNA extraction methods standardized standardized standardized standardized standardized standardized standardized standardized サンプル名 sample name K-Sp-070403-N10-1-1 K-Sp-070403-N10-2-1 K-Sp-070403-N10-3-1 K-Sp-070403-N20-1-1 K-Sp-070403-N20-2-1 K-Sp-070403-N20-3-1 K-Sp-070403-N40-1-1 K-Sp-070403-N40-2-1 種類 target DNA 16S 16S 16S 16S 16S 16S 16S 16S 備考 note DGGE 精度 Denaturing Gradient Gel Electrophoresis standardized or not-standardized standardized standardized standardized standardized standardized standardized standardized standardized 対象マーカー情報 DNA marker information maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium maker for bacterium その他関連データ 変性剤濃度勾配（から） other related data gradient of denaturing agent (from) 50 50 50 50 50 50 50 50 変性剤濃度勾配（まで） gradient of denaturing agent (to) 70 70 70 70 70 70 70 70 泳動電圧 run voltage 50 50 50 50 50 50 50 50 泳動時間 run time 18 18 18 18 18 18 18 18 泳動温度 run temperature 58 58 58 58 58 58 58 58 泳動DNA量 amount of sample DNA 200 200 200 200 200 200 200 200 備考 note 208 209 210 211 212 213 214 215