大項目（日本語） 小項目（日本語） 大項目（英語） 小項目（英語） レーン1 レーン2 レーン3 レーン4 レーン5 レーン6 レーン7 レーン8 レーン9 レーン10 レーン11 レーン12 レーン13 レーン14 レーン15 レーン16 レーン17 レーン18 レーン19 レーン20 参照レーン - reference lane アクセッション番号 アクセッション番号 accession number accession number 000716-2.2_ 000717-2.2* 000721-1.2* 000722-1.3_ 000723-1.2* 000721-2.1_ 000722-2.2* 000723-2.2_ サンプル登録 採取者名 sample registration collector Kazunari Nagaoka Kazunari Nagaoka Kazunari Nagaoka Kazunari Nagaoka Kazunari Nagaoka Kazunari Nagaoka Kazunari Nagaoka Kazunari Nagaoka 所属名 affiliation NARC NARC NARC NARC NARC NARC NARC NARC 採取年月日 sampling date 5/16/2006 5/16/2006 5/16/2006 5/16/2006 5/16/2006 5/16/2006 5/16/2006 5/16/2006 試験目的 試験目的 experimental plan experimental purpose Effect of soil type and organic matter application on soil microbial community. Effect of soil type and organic matter application on soil microbial community. Effect of soil type and organic matter application on soil microbial community. Effect of soil type and organic matter application on soil microbial community. Effect of soil type and organic matter application on soil microbial community. Effect of soil type and organic matter application on soil microbial community. Effect of soil type and organic matter application on soil microbial community. Effect of soil type and organic matter application on soil microbial community. 試験設計 試験設計 experimental plan experimental plan 24plots=4soils*3treatments*2replicates 24plots=4soils*3treatments*2replicates 24plots=4soils*3treatments*2replicates 24plots=4soils*3treatments*2replicates 24plots=4soils*3treatments*2replicates 24plots=4soils*3treatments*2replicates 24plots=4soils*3treatments*2replicates 24plots=4soils*3treatments*2replicates サンプル情報 サンプル情報 sample information sample information continuous application of cattle manure (replicate2) continuous application of dried swine feces (replicate2) continuous application of chemical fertilizer (replicate1) continuous application of cattle manure (replicate1) continuous application of dried swine feces (replicate1) continuous application of chemical fertilizer (replicate1) continuous application of cattle manure (replicate1) continuous application of dried swine feces (replicate1) 試験区面積 試験区面積 experimental plot (m^2) experimental plot 16.75 16.75 16.75 16.75 16.75 16.75 16.75 16.75 採取場所 備考 note artificial concrete framed field (3-1-1 Kannnondai, Tsukuba, Ibaraki) artificial concrete framed field (3-1-1 Kannnondai, Tsukuba, Ibaraki) artificial concrete framed field (3-1-1 Kannnondai, Tsukuba, Ibaraki) artificial concrete framed field (3-1-1 Kannnondai, Tsukuba, Ibaraki) artificial concrete framed field (3-1-1 Kannnondai, Tsukuba, Ibaraki) artificial concrete framed field (3-1-1 Kannnondai, Tsukuba, Ibaraki) artificial concrete framed field (3-1-1 Kannnondai, Tsukuba, Ibaraki) artificial concrete framed field (3-1-1 Kannnondai, Tsukuba, Ibaraki) 由来 由来 origin origin non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere non-rhizosphere 備考 note 採取土壌群 土壌群 soil groups soil groups Gray Upland soils Gray Upland soils Andosols Andosols Andosols Andosols Andosols Andosols 土地利用 土地利用 land use land use upland upland upland upland upland upland upland upland 土壌物理化学性 土性 physical and chemical properties of soil soil texture L L L L L L L L pH (風乾土) pH (dry soil) 5.5 5.8 6.2 6.2 6.6 5.6 5.8 5.8 EC EC (electric conductivity) 炭素含有率 Total C 1.47 1.53 3.26 3.82 3.69 3.42 3.68 3.77 窒素含有率 Total N 0.13 0.15 0.26 0.32 0.32 0.28 0.31 0.33 土壌バイオマス soil biomass 148.9 216.2 215.6 288.2 301.9 250.7 301.5 331.1 可給態P available P 654 1195 281 363 665 238 292 555 窒素無機化量 nitrogen mineralization rates 土壌呼吸活性 soil respiration activity 土壌水分（生土あたりの含水量） soil moisture content 21.7 22.1 33.9 34.7 33.9 33.7 33.7 34 陽イオン交換容量 cation exchange capacity (CEC) その他 the others 栽培作物 作物名 crop crop sweet corn sweet corn sweet corn sweet corn sweet corn sweet corn sweet corn sweet corn 品種 cultivar honey bantam peter corn honey bantam peter corn honey bantam peter corn honey bantam peter corn honey bantam peter corn honey bantam peter corn honey bantam peter corn honey bantam peter corn 栽植密度 planting density (1) 5 5 5 5 5 5 5 5 栽培様式 planting density (2) 60*30 60*30 60*30 60*30 60*30 60*30 60*30 60*30 備考 note 有機質肥料 有機物の種類 organic fertilizer kind of organic material cattle manure dried swine feces non-fertilizer cattle manure dried swine feces non-fertilizer cattle manure dried swine feces 施用量 rate of application 8.5 5.4 8.5 5.4 8.5 5.4 連用年数 years of continuous application 4 4 4 4 4 4 施用時期 time of fertilizer application spring spring spring spring spring spring 備考 note equivqlent to 15kgN/10a equivqlent to 18kgN/10a equivqlent to 15kgN/10a equivqlent to 18kgN/10a equivqlent to 15kgN/10a equivqlent to 18kgN/10a 化学肥料 肥料の種類 chemical fertilizer kind of fertilizer ammonium sulfate, multiphosphate ammonium sulfate ammonium sulfate, multiphosphate, potassium sulfate ammonium sulfate, multiphosphate ammonium sulfate ammonium sulfate, multiphosphate, potassium sulfate ammonium sulfate, multiphosphate ammonium sulfate 施用量(N) rate of fertilizer application(N) 10.5 6 15 10.5 6 15 10.5 6 施用量(P) rate of fertilizer application(P) 5.9 15 5.9 15 5.9 施用量(K) rate of fertilizer application(K) 15 15 連用年数 years of continuous application 4 4 施用時期 time of fertilizer application spring spring spring spring spring spring spring spring 薬剤散布 薬剤の種類 pesticide application kind of pesticide 施用量 rate of pesticide application 連用年数 years of continuous application 施用時期 time of fertilizer application 水管理 水管理 water management water management 試験結果 試験結果 experimental result experimental result Soil samples were collected before fetrilizer and organic matter application. Soil samples were collected before fetrilizer and organic matter application. Soil samples were collected before chemical fetrilizer application. Soil samples were collected before fetrilizer and organic matter application. Soil samples were collected before fetrilizer and organic matter application. Soil samples were collected before chemical fetrilizer application. Soil samples were collected before fetrilizer and organic matter application. Soil samples were collected before fetrilizer and organic matter application. 栽培暦 前作物名 cultivation history former ｃｕｌｔｉｖａｔｅｄ crop carrot carrot carrot carrot carrot carrot carrot carrot 作業内容 seeding or transplanting seeding seeding seeding seeding seeding seeding seeding seeding 作業日 seeding or transplanting date 5/30/2006 5/30/2006 5/30/2006 5/30/2006 5/30/2006 5/30/2006 5/30/2006 5/30/2006 収穫日 harvest date 8/22/2006 8/22/2006 8/22/2006 8/22/2006 8/22/2006 8/22/2006 8/22/2006 8/22/2006 気温 気温 temperature temperature(℃) DNA 抽出法 DNA sequence DNA extraction methods standardized standardized standardized standardized standardized standardized standardized standardized サンプル名 sample name 060516e5-2 060516e6-2 060516f1-2 060516f2-3 060516f3-2 060516f4-1 060516f5-2 060516f6-2 種類 target DNA 18S 18S 18S 18S 18S 18S 18S 18S 備考 note Skim Milk 60mg/g soil Skim Milk60mg/g soil Skim Milk 60mg/g soil Skim Milk 60mg/g soil Skim Milk 60mg/g soil Skim Milk 60mg/g soil DGGE 精度 Denaturing Gradient Gel Electrophoresis standardized or not-standardized standardized standardized standardized standardized standardized standardized standardized standardized 対象マーカー情報 DNA marker information marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus marker for fungus その他関連データ 変性剤濃度勾配（から） other related data gradient of denaturing agent (from) 20 20 20 20 20 20 20 20 変性剤濃度勾配（まで） gradient of denaturing agent (to) 45 45 45 45 45 45 45 45 泳動電圧 run voltage 50 50 50 50 50 50 50 50 泳動時間 run time 20 20 20 20 20 20 20 20 泳動温度 run temperature 60 60 60 60 60 60 60 60 泳動DNA量 amount of sample DNA 200 200 200 200 200 200 200 200 備考 note 719 720 721 722 723 724 725 726