Match to: gi|4502081 Score: 175 amphiphysin isoform 1 [Homo sapiens] Found in search of C:\sequest_txt\021_090903_A.txt Nominal mass (Mr): 76257; Calculated pI value: 4.58 NCBI BLAST search of gi|4502081 against nr Unformatted sequence string for pasting into other applications Taxonomy: Homo sapiens Links to retrieve other entries containing this sequence from NCBI Entrez: gi|1351924 from Homo sapiens gi|2119431 from Homo sapiens gi|550450 from Homo sapiens gi|662992 from Homo sapiens Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 5% Matched peptides shown in Bold Red 1 MADIKTGIFA KNVQKRLNRA QEKVLQKLGK ADETKDEQFE EYVQNFKRQE 51 AEGTRLQREL RGYLAAIKGM QEASMKLTES LHEVYEPDWY GREDVKMVGE 101 KCDVLWEDFH QKLVDGSLLT LDTYLGQFPD IKNRIAKRSR KLVDYDSARH 151 HLEALQSSKR KDESRISKAE EEFQKAQKVF EEFNVDLQEE LPSLWSRRVG 201 FYVNTFKNVS SLEAKFHKEI AVLCHKLYEV MTKLGDQHAD KAFTIQGAPS 251 DSGPLRIAKT PSPPEEPSPL PSPTASPNHT LAPASPAPAR PRSPSQTRKG 301 PPVPPLPKVT PTKELQQENI ISFFEDNFVP EISVTTPSQN EVPEVKKEET 351 LLDLDFDPFK PEVTPAGSAG VTHSPMSQTL PWDLWTTSTD LVQPASGGSF 401 NGFTQPQDTS LFTMQTDQSM ICNLAESEQA PPTEPKAEEP LAAVTPAVGL 451 DLGMDTRAEE PVEEAVIIPG ADADAAVGTL VSAAEGAPGE EAEAEKATVP 501 AGEGVSLEEA KIGTETTEGA ESAQPEAEEL EATVPQEKVI PSVVIEPASN 551 HEEEGENEIT IGAEPKETTE DAAPPGPTSE TPELATEQKP IQDPQPTPSA 601 PAMGAADQLA SAREASQELP PGFLYKVETL HDFEAANSDE LTLQRGDVVL 651 VVPSDSEADQ DAGWLVGVKE SDWLQYRDLA TYKGLFPENF TRRLD
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 2 - 11 553.97 1105.93 1105.30 0.63 1 ADIKTGIFAK Acetyl (N-term) (Ions score 44) 166 - 178 769.24 1536.47 1535.72 0.75 2 ISKAEEEFQKAQK (Ions score 77) 299 - 308 516.10 1030.18 1029.29 0.89 1 KGPPVPPLPK (Ions score 28) 678 - 683 710.58 709.57 709.80 -0.23 0 DLATYK (Ions score 28) LOCUS NP_001626 695 aa linear PRI 26-APR-2004 DEFINITION amphiphysin isoform 1 [Homo sapiens]. ACCESSION NP_001626 VERSION NP_001626.1 GI:4502081 DBSOURCE REFSEQ: accession NM_001635.2 KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (residues 1 to 695) AUTHORS Terada,Y., Tsutsui,K., Sano,K., Hosoya,O., Ohtsuki,H., Tokunaga,A. and Tsutsui,K. TITLE Novel splice variants of amphiphysin I are expressed in retina JOURNAL FEBS Lett. 519 (1-3), 185-190 (2002) PUBMED 12023042 REFERENCE 2 (residues 1 to 695) AUTHORS Butler,M.H., David,C., Ochoa,G.C., Freyberg,Z., Daniell,L., Grabs,D., Cremona,O. and De Camilli,P. TITLE Amphiphysin II (SH3P9; BIN1), a member of the amphiphysin/Rvs family, is concentrated in the cortical cytomatrix of axon initial segments and nodes of ranvier in brain and around T tubules in skeletal muscle JOURNAL J. Cell Biol. 137 (6), 1355-1367 (1997) PUBMED 9182667 REFERENCE 3 (residues 1 to 695) AUTHORS Yamamoto,R., Li,X., Winter,S., Francke,U. and Kilimann,M.W. TITLE Primary structure of human amphiphysin, the dominant autoantigen of paraneoplastic stiff-man syndrome, and mapping of its gene (AMPH) to chromosome 7p13-p14 JOURNAL Hum. Mol. Genet. 4 (2), 265-268 (1995) PUBMED 7757077 REFERENCE 4 (residues 1 to 695) AUTHORS David,C., Solimena,M. and De Camilli,P. TITLE Autoimmunity in stiff-Man syndrome with breast cancer is targeted to the C-terminal region of human amphiphysin, a protein similar to the yeast proteins, Rvs167 and Rvs161 JOURNAL FEBS Lett. 351 (1), 73-79 (1994) PUBMED 8076697 REFERENCE 5 (residues 1 to 695) AUTHORS De Camilli,P., Thomas,A., Cofiell,R., Folli,F., Lichte,B., Piccolo,G., Meinck,H.M., Austoni,M., Fassetta,G., Bottazzo,G. et al. TITLE The synaptic vesicle-associated protein amphiphysin is the 128-kD autoantigen of Stiff-Man syndrome with breast cancer JOURNAL J. Exp. Med. 178 (6), 2219-2223 (1993) PUBMED 8245793 REFERENCE 6 (residues 1 to 695) AUTHORS Lichte,B., Veh,R.W., Meyer,H.E. and Kilimann,M.W. TITLE Amphiphysin, a novel protein associated with synaptic vesicles JOURNAL EMBO J. 11 (7), 2521-2530 (1992) PUBMED 1628617 COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X81438.1 and AF034996.1. Summary: This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. Transcript Variant: This variant (1) encodes the longer isoform. FEATURES Location/Qualifiers source 1..695 /organism="Homo sapiens" /db_xref="taxon:9606" /chromosome="7" /map="7p14-p13" Protein 1..695 /product="amphiphysin isoform 1" CDS 1..695 /gene="AMPH" /coded_by="NM_001635.2:70..2157" /note="isoform 1 is encoded by transcript variant 1; go_component: synaptic vesicle [goid 0008021] [evidence TAS] [pmid 1628617]; go_component: actin cytoskeleton [goid 0015629] [evidence TAS] [pmid 9182667]; go_process: endocytosis [goid 0006897] [evidence TAS] [pmid 9182667]; go_process: synaptic transmission [goid 0007268] [evidence IEA]; go_process: synaptic transmission [goid 0007268] [evidence TAS] [pmid 9182667]" /db_xref="GeneID:273" /db_xref="LocusID:273" /db_xref="MIM:600418"