Mascot Search Results

Protein View

Match to: gi|13529464 Score: 183
Nucleolin [Mus musculus]
Found in search of C:\Xcalibur\sequest\061_A080504_7.txt

Nominal mass (Mr): 76779; Calculated pI value: 4.70
NCBI BLAST search of gi|13529464 against nr
Unformatted sequence string for pasting into other applications

Taxonomy: Mus musculus

Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Sequence Coverage: 5%

Matched peptides shown in Bold Red

     1 MVKLAKAGKT HGEAKKMAPP PKEVEEDSED EEMSEDEDDS SGEEEVVIPQ 
    51 KKGKKATTTP AKKVVVSQTK KAAVPTPAKK AAVTPGKKAV ATPAKKNITP 
   101 AKVIPTPGKK GAAQAKALVP TPGKKGAATP AKGAKNGKNA KKEDSDEDED 
   151 EEDEEDSDED EDDEEEDEFE PPIVKGVKPA KAAPAAPASE DEEDDEDEDD 
   201 EEEEDEEEED DSEEEVMEIT TAKGKKTPAK VVPMKAKSVA EEEDEEEEDE 
   251 DDEDEDDEEE DDEDDDEEEE EEEPVKAAPG KRKKEMTKQK EAPEAKKQKV 
   301 EGSEPTTPFN LFIGNLNPNK SVNELKFAIS ELFAKNDLAV VDVRTGTNRK 
   351 FGYVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK 
   401 NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE 
   451 KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK 
   501 ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE 
   551 GRTIRLELQG SNSRSQPSKT LFVKGLSEDT TEETLKESFE GSVRARIVTD 
   601 RETGSSKGFG FVDFNSEEDA KAAKEAMEDG EIDGNKVTLD WAKPKGEGGF 
   651 GGRGGGRGGF GGRGGGRGGR GGFGGRGRGG FGGRGGFRGG RGGGGDFKPQ 
   701 GKKTKFE

  Residue Number  Increasing Mass  Decreasing Mass 

 Start - End   Observed  Mr(expt) Mr(calc)   Delta   Miss Sequence
   488 - 500     699.50  1396.99  1396.60     0.39     0  TLVLSNLSYSATK  (Ions score 84)
   501 - 509     562.82  1123.63  1123.23     0.41     0  ETLEEVFEK  (Ions score 34)
   608 - 621     781.86  1561.71  1561.62     0.08     0  GFGFVDFNSEEDAK  (Ions score 65)

Error Distribution


LOCUS AAH05460 707 aa linear ROD 29-JUN-2004 DEFINITION Nucleolin [Mus musculus]. ACCESSION AAH05460 VERSION AAH05460.1 GI:13529464 DBSOURCE accession BC005460.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 707) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 707) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (27-MAR-2001) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov/ COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Gilbert Smith, Ph.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Sequencing Group at the Stanford Human Genome Center, Stanford University School of Medicine, Stanford, CA 94305 Web site: http://www-shgc.stanford.edu/ Contact: (Dickson, Mark) mcd@paxil.stanford.edu Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, R. M. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov/ Series: IRAK Plate: 8 Row: d Column: 2 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 7106376. Method: conceptual translation. FEATURES Location/Qualifiers source 1..707 /organism="Mus musculus" /strain="CZECH II" /db_xref="taxon:10090" /clone="MGC:6363 IMAGE:3495665" /tissue_type="Mammary tumor metastatized to lung. Tumor arose spontaneously from a senescent normal mammary (clonal) outgrowth infected with the virus MMTV." /clone_lib="NCI_CGAP_Lu29" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" Protein 1..707 /product="nucleolin" CDS 1..707 /gene="Ncl" /coded_by="BC005460.1:53..2176" /db_xref="LocusID:17975" /db_xref="MGI:97286"

Mascot:  http://www.matrixscience.com/