Mascot Search Results

Protein View

Match to: gi|6680572 Score: 1239
kinesin family member 5B; kinesin heavy chain member 5B, ubiquitous; kinesin heavy chain [Mus musculus]
Found in search of C:\sequest_txt\043_030104_2.txt

Nominal mass (Mr): 109549; Calculated pI value: 5.96
NCBI BLAST search of gi|6680572 against nr
Unformatted sequence string for pasting into other applications

Taxonomy: Mus musculus
Links to retrieve other entries containing this sequence from NCBI Entrez:
gi|2497519 from Mus musculus
gi|2062607 from Mus musculus

Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Sequence Coverage: 22%

Matched peptides shown in Bold Red

     1 MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVV IASKPYAFDR 
    51 VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH 
   101 DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK 
   151 TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH 
   201 SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE 
   251 AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI 
   301 CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK 
   351 NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDIAITS 
   401 DKGAAAVGMA GSFTDAERRK CEEELAKLYK QLDDKDEEIN QQSQLVEKLK 
   451 TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV 
   501 NYDQKSQEVE DKTKEYELLT DEFNQKSATL ASIDAELQKL KEMTNHQKKR 
   551 AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE 
   601 VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL 
   651 QNDEQKKRQL EESLDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA 
   701 VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLETERLRVE 
   751 HERLKATDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR 
   801 KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV 
   851 RDNADLRCEL PKLEFRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD 
   901 RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGTV RGGGSFVQNN 
   951 QPVGLRGGGG KQS

  Residue Number  Increasing Mass  Decreasing Mass 

 Start - End   Observed  Mr(expt) Mr(calc)   Delta   Miss Sequence
    15 - 25      681.13  1360.25  1360.49    -0.24     0  FRPLNESEVNR  (Ions score 34)
    15 - 28      555.02  1662.03  1660.81     1.22     1  FRPLNESEVNRGDK  (Ions score 31)
    99 - 110     668.33  1334.65  1334.56     0.09     0  LHDPEGMGIIPR  (Ions score 64)
   191 - 203     741.63  1481.25  1481.61    -0.36     0  HVAVTNMNEHSSR  (Ions score 86)
   204 - 213     579.78  1157.54  1157.38     0.16     0  SHSIFLINVK  (Ions score 49)
   241 - 252     581.07  1160.12  1160.25    -0.12     0  TGAEGAVLDEAK  (Ions score 93)
   370 - 384     875.41  1748.80  1748.86    -0.06     1  NGETVPIDEQFDKEK  (Ions score 54)
   451 - 464     818.27  1634.53  1634.82    -0.30     0  TQMLDQEELLASTR  (Ions score 87)
   466 - 476     667.50  1332.98  1333.40    -0.41     0  DQDNMQAELNR  (Ions score 57)
   561 - 575     764.73  1527.45  1527.70    -0.24     0  DLAEIGIAVGNNDVK  (Ions score 113)
   699 - 709     663.03  1324.05  1323.43     0.62     0  QAVEQQIQSHR  (Ions score 60)
   715 - 726     688.37  1374.73  1374.51     0.22     1  QISSLRDEVEAK  (Ions score 49)
   729 - 739     658.79  1315.56  1315.45     0.11     0  LITDLQDQNQK  (Ions score 76)
   784 - 791     846.60   845.59   845.95    -0.35     0  GLEETVAK  (Ions score 26)
   792 - 800     562.98  1123.94  1123.28     0.66     0  ELQTLHNLR  (Ions score 45)
   801 - 810     596.35  1190.68  1190.41     0.27     1  KLFVQDLATR  (Ions score 89)
   802 - 810     532.27  1062.52  1062.23     0.28     0  LFVQDLATR  (Ions score 75)
   814 - 829     769.64  1537.26  1537.51    -0.25     0  SAEVDSDDTGGSAAQK  (Ions score 86)
   832 - 844     775.29  1548.56  1548.76    -0.19     0  ISFLENNLEQLTK  (Ions score 66)
   942 - 956     765.72  1529.42  1529.68    -0.26     0  GGGSFVQNNQPVGLR  (Ions score 57)

Error Distribution


LOCUS NP_032474 963 aa linear ROD 24-JUN-2004 DEFINITION kinesin family member 5B; kinesin heavy chain member 5B, ubiquitous; kinesin heavy chain [Mus musculus]. ACCESSION NP_032474 VERSION NP_032474.1 GI:6680572 DBSOURCE REFSEQ: accession NM_008448.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 963) AUTHORS Semiz,S., Park,J.G., Nicoloro,S.M., Furcinitti,P., Zhang,C., Chawla,A., Leszyk,J. and Czech,M.P. TITLE Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules JOURNAL EMBO J. 22 (10), 2387-2399 (2003) PUBMED 12743033 REMARK GeneRIF: data reveal that insulin signaling targets the engagement or initiates the movement of glutamate transporter 4-containing membranes on microtubules via KIF5B through a phosphatidylinositol 3-kinase-independent mechanism. REFERENCE 2 (residues 1 to 963) AUTHORS Plitz,T. and Pfeffer,K. TITLE Intact lysosome transport and phagosome function despite kinectin deficiency JOURNAL Mol. Cell. Biol. 21 (17), 6044-6055 (2001) PUBMED 11486041 REFERENCE 3 (residues 1 to 963) AUTHORS Kanai,Y., Okada,Y., Tanaka,Y., Harada,A., Terada,S. and Hirokawa,N. TITLE KIF5C, a novel neuronal kinesin enriched in motor neurons JOURNAL J. Neurosci. 20 (17), 6374-6384 (2000) PUBMED 10964943 REFERENCE 4 (residues 1 to 963) AUTHORS Tanaka,Y., Kanai,Y., Okada,Y., Nonaka,S., Takeda,S., Harada,A. and Hirokawa,N. TITLE Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria JOURNAL Cell 93 (7), 1147-1158 (1998) PUBMED 9657148 REFERENCE 5 (residues 1 to 963) AUTHORS Meng,Y.X., Wilson,G.W., Avery,M.C., Varden,C.H. and Balczon,R. TITLE Suppression of the expression of a pancreatic beta-cell form of the kinesin heavy chain by antisense oligonucleotides inhibits insulin secretion from primary cultures of mouse beta-cells JOURNAL Endocrinology 138 (5), 1979-1987 (1997) PUBMED 9112396 REFERENCE 6 (residues 1 to 963) AUTHORS Gudkov,A.V., Kazarov,A.R., Thimmapaya,R., Axenovich,S.A., Mazo,I.A. and Roninson,I.B. TITLE Cloning mammalian genes by expression selection of genetic suppressor elements: association of kinesin with drug resistance and cell immortalization JOURNAL Proc. Natl. Acad. Sci. U.S.A. 91 (9), 3744-3748 (1994) PUBMED 8170981 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U86090.1. FEATURES Location/Qualifiers source 1..963 /organism="Mus musculus" /db_xref="taxon:10090" /chromosome="18" /map="18 1.0 cM" Protein 1..963 /product="kinesin family member 5B" /function="motor protein" /note="kinesin heavy chain member 5B, ubiquitous" Region 8..323 /region_name="Kinesin motor, catalytic domain" /note="KISc" /db_xref="CDD:14817" Region <422..908 /region_name="HOOK protein" /note="HOOK" /db_xref="CDD:23846" Region 678..907 /region_name="Intermediate filament protein" /note="Filament" /db_xref="CDD:16593" CDS 1..963 /gene="Kif5b" /coded_by="NM_008448.1:245..3136" /note="go_component: kinesin complex [goid 0005871] [evidence TAS] [pmid 10964943]; go_component: microtubule associated complex [goid 0005875] [evidence IEA]; go_function: ATP binding [goid 0005524] [evidence IEA]; go_function: motor activity [goid 0003774] [evidence IEA]; go_function: protein binding [goid 0005515] [evidence IPI] [pmid 10964943]; go_function: microtubule motor activity [goid 0003777] [evidence IEA]; go_function: microtubule motor activity [goid 0003777] [evidence TAS] [pmid 10964943]; go_process: mitochondrial transport [goid 0006839] [evidence TAS] [pmid 11486041]; go_process: microtubule-based process [goid 0007017] [evidence TAS] [pmid 11486041]; go_process: cytoplasm organization and biogenesis [goid 0007028] [evidence IMP] [pmid 9657148]" /db_xref="GeneID:16573" /db_xref="LocusID:16573" /db_xref="MGI:1098268"

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