Match to: gi|29437175 Score: 92 Nsep1 protein [Mus musculus] Found in search of C:\Xcalibur\sequest\061_A080604_13.txt Nominal mass (Mr): 35756; Calculated pI value: 9.87 NCBI BLAST search of gi|29437175 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 5% Matched peptides shown in Bold Red 1 MISEAETQQP PAAPAAALSA ADTKPGSTGS GAGSGGPGGL TSAAPAGGDK 51 KVIATKVLGT VKWFNVRNGY GFINRNDTKE DVFVHQTAIK KNNPRKYLRS 101 VGDGETVEFD VVEGEKGAEA ANVTGPGGVP VQGSKYAADR NHYRRYPRRR 151 GPPRNYQQNY QNSESGEKNE GSESAPEGQA QQRRPYRRRR FPPYYMRRPY 201 ARRPQYSNPP VQGEVMEGAD NQGAGEQGRP VRQNMYRGYR PRFRRGPPRQ 251 RQPREDGNEE DKENQGDETQ GQQPPQRRYR RNFNYRRRRP ENPKPQDGKE 301 TKAADPPAEN SSAPEAEQGG AE
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 117 - 135 848.93 1695.84 1695.85 -0.01 0 GAEAANVTGPGGVPVQGSK (Ions score 92) LOCUS AAH49977 322 aa linear ROD 07-OCT-2003 DEFINITION Nsep1 protein [Mus musculus]. ACCESSION AAH49977 VERSION AAH49977.1 GI:29437175 DBSOURCE accession BC049977.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 322) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REFERENCE 2 (residues 1 to 322) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (31-MAR-2003) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov/ COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Jeffrey E. Green, M.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: National Institutes of Health Intramural Sequencing Center (NISC), Gaithersburg, Maryland; Web site: http://www.nisc.nih.gov/ Contact: nisc_mgc@nhgri.nih.gov Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B., Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S., Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P., Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R., Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C., McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W., Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L., Young,A., Zhang,L.-H. and Green,E.D. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov/ Series: IRAK Plate: 108 Row: e Column: 12 This clone was selected for full length sequencing because it passed the following selection criteria: GenomeScan gene prediction, Similarity but not identity to protein. Method: conceptual translation. FEATURES Location/Qualifiers source 1..322 /organism="Mus musculus" /strain="FVB/N" /db_xref="taxon:10090" /clone="MGC:58972 IMAGE:4922840" /tissue_type="Salivary gland, 10 week old female mouse" /clone_lib="NCI_CGAP_SG2" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" Protein 1..322 /product="Nsep1 protein" Region 56..126 /region_name="Cold Shock RNA binding domain of the OB fold" /note="cold" /db_xref="CDD:LOAD_cold" CDS 1..322 /gene="Nsep1" /coded_by="BC049977.1:125..1093" /db_xref="LocusID:22608" /db_xref="MGI:99146"