Match to: gi|27369782 Score: 656 gem (nuclear organelle) associated protein 5; hypothetical protein C330013N08 [Mus musculus] Found in search of C:\Xcalibur\sequest\059_B071604_4.txt Nominal mass (Mr): 166562; Calculated pI value: 6.25 NCBI BLAST search of gi|27369782 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi|34921723 from Mus musculus gi|26339902 from Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 8% Matched peptides shown in Bold Red 1 MKPEPRTLPP SPNWYCSRCS DAAPGGIFGF AARTSVFLVR VGPGAGASPG 51 APPFRVVGEL VGHTERVSGF TFSHHPGQYN LCATSSDDGT VKVWDVETKT 101 VVTEHTLHQH TISALHWSPT VKDLIVSGDE KGVVFCYWLN RNDSQHLFTE 151 PRTIFCLTCS PHHENLVAIG YKDGIVVIID ISKKGEVIHR LRGHDDEIHS 201 IAWCPLSGED CLSISQEENS EEPDIPNGKL IAETPITKGC YLATGSKDQT 251 IRIWSCSRGR GVMVLKLPFL KRRSGGVDPT VKERLWLTLH WPKNQPTQLV 301 SSCFGGELLL WDLTQSWRRK YTLFSTSAEG HNHSRIVFNL CSLKTEDGKQ 351 LLLSTSMDRD VKCWDMATLE CCWTLPSLGG FAYSLAFSPV DVGSLAIGVG 401 DGMIRVWNTL SIKNNYDVKN FWQGVKSKVT ALCWHPNKEG CLAFGTDDGK 451 VGLYDTCSNK PPQISSTYHK KTVYRLAWGP PVPPMSLGGE GDRPSLTLYS 501 CGGEGVVLQH NPWKLSGEAF DINKLVRDTN SIRYKLPVHT EISWKGDGKV 551 LALGNEDGSI EIFQVPNLRL LCTIQQHHKL VNAIVWHHEH GSRPELSCLL 601 ASGSNNAVIY VHNLKAVLES NPESPITITE PYRTLSGHTA KITSLAWSPH 651 HDGRLVSACY DGTAQVWDAL REEPLFNFRG HRGRLLCVAW SPVDPECIYS 701 GADDFCVYRW LTSMQDHSRP PQGKKCIELE KKRLSQFKPK LKKKKKPTLR 751 LPVKQDSSVG NEDESVKENS GPAENGLSDQ DGEEEAQEPE LPPSPVVCVE 801 PVSCTDICSG FEKSKVTVSS KATSLKKEPA KEKPEALLKK RKARSMLPLS 851 TSLDHRSKEE LHRDCLVLAT ATHAKAELNE DVSADLEERF HLGLFTDRAT 901 LYRMMETEGK GHLESGHPEL FHQLMLWKGD LKGVLQAAAE RGELTDSLVA 951 VAPVAGYSVW LWAVEAFAKQ LCFQDQYVKA ASYLLSIHKV YEAVELLKSN 1001 HLYREAIAVA KARLRPEDPV LKELYLSWGS ILERDGHYAI AAKCYLGATS 1051 AYDAAKVLAR KGDAASLRTA AELAAIAGEH ELAASLALRC AQELLLMKNW 1101 VGAQEALGLH ESLQGQRLVF CLLELLCRHL EEKQPLEVRG PSSIYHQWAT 1151 GSEGTLVQRV TGVWRSAFSV DTPEQCQAAL QKLQDVKYPS ATSNTPFRQL 1201 LLHVCHDLTL AMLSQQAAAW EEAVPALLQA VVRSYASGNF TLMQEIYSAF 1251 LPGGCDHLRD KLGDLSPAMA AYKSLEAFCI YGQLYEVWWS LCGPGPESSV 1301 WVLSAESTVS DKQSKPEDSA SAEDMEQPPG PGPRLSAESE RLLSACKELF 1351 SERHASLQTS QRTVAEVQET LAEMIRQHQK SQLCKATTNG PSRDEPSRDE 1401 PSQEAERAPS QPPSPTEERN APVSLPELTR RLTEANERIA EFPESVKAWP 1451 FPDVLECCLV LLHIGSQCPD AVDPEMQQQA QELLHKYGHT RAYRRHCQSR 1501 HT
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 34 - 40 411.70 821.39 820.99 0.41 0 TSVFLVR (Ions score 39) 41 - 55 669.87 1337.72 1337.50 0.21 0 VGPGAGASPGAPPFR (Ions score 59) 56 - 66 598.74 1195.46 1195.34 0.12 0 VVGELVGHTER (Ions score 68) 142 - 152 672.79 1343.56 1343.42 0.15 0 NDSQHLFTEPR (Ions score 72) 515 - 524 547.70 1093.38 1093.20 0.18 0 LSGEAFDINK (Ions score 80) 710 - 724 884.92 1767.83 1767.98 -0.15 0 WLTSMQDHSRPPQGK (Ions score 43) 845 - 856 679.57 1357.13 1356.56 0.57 0 SMLPLSTSLDHR (Ions score 51) 980 - 989 552.19 1102.37 1102.30 0.07 0 AASYLLSIHK (Ions score 65) 1262 - 1273 619.25 1236.49 1236.45 0.04 0 LGDLSPAMAAYK (Ions score 66) 1363 - 1376 795.66 1589.31 1589.82 -0.51 0 TVAEVQETLAEMIR (Ions score 103) 1420 - 1430 599.04 1196.06 1196.37 -0.31 0 NAPVSLPELTR (Ions score 49) LOCUS NP_766146 1502 aa linear ROD 22-DEC-2003 DEFINITION gem (nuclear organelle) associated protein 5; hypothetical protein C330013N08 [Mus musculus]. ACCESSION NP_766146 VERSION NP_766146.1 GI:27369782 DBSOURCE REFSEQ: accession NM_172558.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 AUTHORS The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I & II Team. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420, 563-573 (2002) REFERENCE 2 AUTHORS Kawai,J., Shinagawa,A., Shibata,K., Yoshino,M., Itoh,M., Ishii,Y., Arakawa,T., Hara,A., Fukunishi,Y., Konno,H., Adachi,J., Fukuda,S., Aizawa,K., Izawa,M., Nishi,K., Kiyosawa,H., Kondo,S., Yamanaka,I., Saito,T., Okazaki,Y., Gojobori,T., Bono,H., Kasukawa,T., Saito,R., Kadota,K., Matsuda,H., Ashburner,M., Batalov,S., Casavant,T., Fleischmann,W., Gaasterland,T., Gissi,C., King,B., Kochiwa,H., Kuehl,P., Lewis,S., Matsuo,Y., Nikaido,I., Pesole,G., Quackenbush,J., Schriml,L.M., Staubli,F., Suzuki,R., Tomita,M., Wagner,L., Washio,T., Sakai,K., Okido,T., Furuno,M., Aono,H., Baldarelli,R., Barsh,G., Blake,J., Boffelli,D., Bojunga,N., Carninci,P., de Bonaldo,M.F., Brownstein,M.J., Bult,C., Fletcher,C., Fujita,M., Gariboldi,M., Gustincich,S., Hill,D., Hofmann,M., Hume,D.A., Kamiya,M., Lee,N.H., Lyons,P., Marchionni,L., Mashima,J., Mazzarelli,J., Mombaerts,P., Nordone,P., Ring,B., Ringwald,M., Rodriguez,I., Sakamoto,N., Sasaki,H., Sato,K., Schonbach,C., Seya,T., Shibata,Y., Storch,K.F., Suzuki,H., Toyo-oka,K., Wang,K.H., Weitz,C., Whittaker,C., Wilming,L., Wynshaw-Boris,A., Yoshida,K., Hasegawa,Y., Kawaji,H., Kohtsuki,S. and Hayashizaki,Y. TITLE Functional annotation of a full-length mouse cDNA collection JOURNAL Nature 409 (6821), 685-690 (2001) PUBMED 11217851 REFERENCE 3 AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N., Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai,T., Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M., Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S., Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K. and Togawa,Y. TITLE RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer JOURNAL Genome Res. 10 (11), 1757-1771 (2000) PUBMED 11076861 REFERENCE 4 AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K., Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y. TITLE Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes JOURNAL Genome Res. 10 (10), 1617-1630 (2000) PUBMED 11042159 REFERENCE 5 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency full-length cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) PUBMED 10349636 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK049216.1. FEATURES Location/Qualifiers source 1..1502 /organism="Mus musculus" /strain="C57BL/6J" /db_xref="taxon:10090" /chromosome="11" Protein 1..1502 /product="gem (nuclear organelle) associated protein 5" /note="hypothetical protein C330013N08" Region 57..408 /region_name="WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly" /note="WD40" /db_xref="CDD:5398" Region 329..668 /region_name="WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly" /note="WD40" /db_xref="CDD:5398" Region 631..>710 /region_name="WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly" /note="WD40" /db_xref="CDD:5398" CDS 1..1502 /gene="Gemin5" /coded_by="NM_172558.1:22..4530" /note="go_component: spliceosome complex [goid 0005681] [evidence IEA]; go_component: nucleus [goid 0005634] [evidence IEA]; go_process: mRNA processing [goid 0006397] [evidence IEA]; go_process: nuclear mRNA splicing, via spliceosome [goid 0000398] [evidence IEA]" /db_xref="GeneID:216766" /db_xref="LocusID:216766" /db_xref="MGI:2449311"