Match to: gi|31560792 Score: 345 SH3-domain GRB2-like 2; SH3 domain protein 2A; endophilin I [Mus musculus] Found in search of C:\Xcalibur\sequest\059_B071504_13.txt Nominal mass (Mr): 39955; Calculated pI value: 5.26 NCBI BLAST search of gi|31560792 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi|17225449 from Mus musculus gi|17225451 from Mus musculus gi|17390906 from Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 20% Matched peptides shown in Bold Red 1 MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI 51 MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF 101 GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD 151 LREIQHHLKK LEGRRLDFDY KKKRQGKIPD EELRQALEKF DESKEIAESS 201 MFNLLEMDIE QVSQLSALVQ AQLEYHKQAV QILQQVTVRL EERIRQASSQ 251 PRREYQPKPR MSLEFATGDS TQPNGGLSHT GTPKPPGVQM DQPCCRALYD 301 FEPENEGELG FKEGDIITLT NQIDENWYEG MLHGQSGFFP INYVEILVAL 351 PH
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 54 - 65 695.13 1388.24 1388.54 -0.30 0 TIEYLQPNPASR (Ions score 71) 83 - 99 888.40 1774.77 1775.05 -0.28 0 GPGYPQAEALLAEAMLK Oxidation (M) (Ions score 76) 123 - 136 820.39 1638.77 1637.82 0.95 1 ELSEVKDSLDMEVK Oxidation (M) (Ions score 52) 228 - 239 692.29 1382.56 1382.63 -0.07 0 QAVQILQQVTVR (Ions score 93) 297 - 312 929.98 1857.95 1857.99 -0.04 0 ALYDFEPENEGELGFK (Ions score 62) LOCUS NP_062408 352 aa linear ROD 22-DEC-2003 DEFINITION SH3-domain GRB2-like 2; SH3 domain protein 2A; endophilin I [Mus musculus]. ACCESSION NP_062408 VERSION NP_062408.2 GI:31560792 DBSOURCE REFSEQ: accession NM_019535.2 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 352) AUTHORS Fehr,C., Shirley,R.L., Belknap,J.K., Crabbe,J.C. and Buck,K.J. TITLE Congenic mapping of alcohol and pentobarbital withdrawal liability loci to a <1 centimorgan interval of murine chromosome 4: identification of Mpdz as a candidate gene JOURNAL J. Neurosci. 22 (9), 3730-3738 (2002) PUBMED 11978849 REFERENCE 2 (residues 1 to 352) AUTHORS Micheva,K.D., Kay,B.K. and McPherson,P.S. TITLE Synaptojanin forms two separate complexes in the nerve terminal. Interactions with endophilin and amphiphysin JOURNAL J. Biol. Chem. 272 (43), 27239-27245 (1997) PUBMED 9341169 REFERENCE 3 (residues 1 to 352) AUTHORS Micheva,K.D., Ramjaun,A.R., Kay,B.K. and McPherson,P.S. TITLE SH3 domain-dependent interactions of endophilin with amphiphysin JOURNAL FEBS Lett. 414 (2), 308-312 (1997) PUBMED 9315708 REFERENCE 4 (residues 1 to 352) AUTHORS Hoffman,N.G., Sparks,A.B., Carter,J.M. and Kay,B.K. TITLE Binding properties of SH3 peptide ligands identified from phage-displayed random peptide libraries JOURNAL Mol. Divers. 2 (1-2), 5-12 (1996) PUBMED 9238627 REFERENCE 5 (residues 1 to 352) AUTHORS Sparks,A.B., Hoffman,N.G., McConnell,S.J., Fowlkes,D.M. and Kay,B.K. TITLE Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins JOURNAL Nat. Biotechnol. 14 (6), 741-744 (1996) PUBMED 9630982 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC018385.1. On Jun 10, 2003 this sequence version replaced gi:9507095. FEATURES Location/Qualifiers source 1..352 /organism="Mus musculus" /db_xref="taxon:10090" /chromosome="4" /map="4 41.6 cM" Protein 1..352 /product="SH3-domain GRB2-like 2" /note="SH3 domain protein 2A; endophilin I" Region 5..240 /region_name="BAR domain" /note="BAR" /db_xref="CDD:9026" Region 6..352 /region_name="Lysophosphatidic acid acyltransferase endophilin/SH3GL, involved in synaptic vesicle formation [Lipid transport and metabolism, Signal transduction mechanisms]" /note="KOG1118" /db_xref="CDD:18909" Region 291..347 /region_name="Src homology 3 domains" /note="SH3" /db_xref="CDD:22760" CDS 1..352 /gene="Sh3gl2" /coded_by="NM_019535.2:122..1180" /note="go_component: kinesin complex [goid 0005871] [evidence IEA]; go_function: protein binding [goid 0005515] [evidence IDA] [pmid 9630982]" /db_xref="GeneID:20404" /db_xref="LocusID:20404" /db_xref="MGI:700009"