Match to: gi|6680572 Score: 1239 kinesin family member 5B; kinesin heavy chain member 5B, ubiquitous; kinesin heavy chain [Mus musculus] Found in search of C:\sequest_txt\043_030104_2.txt Nominal mass (Mr): 109549; Calculated pI value: 5.96 NCBI BLAST search of gi|6680572 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi|2497519 from Mus musculus gi|2062607 from Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 22% Matched peptides shown in Bold Red 1 MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVV IASKPYAFDR 51 VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH 101 DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK 151 TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH 201 SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE 251 AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI 301 CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK 351 NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDIAITS 401 DKGAAAVGMA GSFTDAERRK CEEELAKLYK QLDDKDEEIN QQSQLVEKLK 451 TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV 501 NYDQKSQEVE DKTKEYELLT DEFNQKSATL ASIDAELQKL KEMTNHQKKR 551 AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE 601 VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL 651 QNDEQKKRQL EESLDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA 701 VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLETERLRVE 751 HERLKATDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR 801 KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV 851 RDNADLRCEL PKLEFRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD 901 RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGTV RGGGSFVQNN 951 QPVGLRGGGG KQS
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 15 - 25 681.13 1360.25 1360.49 -0.24 0 FRPLNESEVNR (Ions score 34) 15 - 28 555.02 1662.03 1660.81 1.22 1 FRPLNESEVNRGDK (Ions score 31) 99 - 110 668.33 1334.65 1334.56 0.09 0 LHDPEGMGIIPR (Ions score 64) 191 - 203 741.63 1481.25 1481.61 -0.36 0 HVAVTNMNEHSSR (Ions score 86) 204 - 213 579.78 1157.54 1157.38 0.16 0 SHSIFLINVK (Ions score 49) 241 - 252 581.07 1160.12 1160.25 -0.12 0 TGAEGAVLDEAK (Ions score 93) 370 - 384 875.41 1748.80 1748.86 -0.06 1 NGETVPIDEQFDKEK (Ions score 54) 451 - 464 818.27 1634.53 1634.82 -0.30 0 TQMLDQEELLASTR (Ions score 87) 466 - 476 667.50 1332.98 1333.40 -0.41 0 DQDNMQAELNR (Ions score 57) 561 - 575 764.73 1527.45 1527.70 -0.24 0 DLAEIGIAVGNNDVK (Ions score 113) 699 - 709 663.03 1324.05 1323.43 0.62 0 QAVEQQIQSHR (Ions score 60) 715 - 726 688.37 1374.73 1374.51 0.22 1 QISSLRDEVEAK (Ions score 49) 729 - 739 658.79 1315.56 1315.45 0.11 0 LITDLQDQNQK (Ions score 76) 784 - 791 846.60 845.59 845.95 -0.35 0 GLEETVAK (Ions score 26) 792 - 800 562.98 1123.94 1123.28 0.66 0 ELQTLHNLR (Ions score 45) 801 - 810 596.35 1190.68 1190.41 0.27 1 KLFVQDLATR (Ions score 89) 802 - 810 532.27 1062.52 1062.23 0.28 0 LFVQDLATR (Ions score 75) 814 - 829 769.64 1537.26 1537.51 -0.25 0 SAEVDSDDTGGSAAQK (Ions score 86) 832 - 844 775.29 1548.56 1548.76 -0.19 0 ISFLENNLEQLTK (Ions score 66) 942 - 956 765.72 1529.42 1529.68 -0.26 0 GGGSFVQNNQPVGLR (Ions score 57) LOCUS NP_032474 963 aa linear ROD 24-JUN-2004 DEFINITION kinesin family member 5B; kinesin heavy chain member 5B, ubiquitous; kinesin heavy chain [Mus musculus]. ACCESSION NP_032474 VERSION NP_032474.1 GI:6680572 DBSOURCE REFSEQ: accession NM_008448.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 963) AUTHORS Semiz,S., Park,J.G., Nicoloro,S.M., Furcinitti,P., Zhang,C., Chawla,A., Leszyk,J. and Czech,M.P. TITLE Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules JOURNAL EMBO J. 22 (10), 2387-2399 (2003) PUBMED 12743033 REMARK GeneRIF: data reveal that insulin signaling targets the engagement or initiates the movement of glutamate transporter 4-containing membranes on microtubules via KIF5B through a phosphatidylinositol 3-kinase-independent mechanism. REFERENCE 2 (residues 1 to 963) AUTHORS Plitz,T. and Pfeffer,K. TITLE Intact lysosome transport and phagosome function despite kinectin deficiency JOURNAL Mol. Cell. Biol. 21 (17), 6044-6055 (2001) PUBMED 11486041 REFERENCE 3 (residues 1 to 963) AUTHORS Kanai,Y., Okada,Y., Tanaka,Y., Harada,A., Terada,S. and Hirokawa,N. TITLE KIF5C, a novel neuronal kinesin enriched in motor neurons JOURNAL J. Neurosci. 20 (17), 6374-6384 (2000) PUBMED 10964943 REFERENCE 4 (residues 1 to 963) AUTHORS Tanaka,Y., Kanai,Y., Okada,Y., Nonaka,S., Takeda,S., Harada,A. and Hirokawa,N. TITLE Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria JOURNAL Cell 93 (7), 1147-1158 (1998) PUBMED 9657148 REFERENCE 5 (residues 1 to 963) AUTHORS Meng,Y.X., Wilson,G.W., Avery,M.C., Varden,C.H. and Balczon,R. TITLE Suppression of the expression of a pancreatic beta-cell form of the kinesin heavy chain by antisense oligonucleotides inhibits insulin secretion from primary cultures of mouse beta-cells JOURNAL Endocrinology 138 (5), 1979-1987 (1997) PUBMED 9112396 REFERENCE 6 (residues 1 to 963) AUTHORS Gudkov,A.V., Kazarov,A.R., Thimmapaya,R., Axenovich,S.A., Mazo,I.A. and Roninson,I.B. TITLE Cloning mammalian genes by expression selection of genetic suppressor elements: association of kinesin with drug resistance and cell immortalization JOURNAL Proc. Natl. Acad. Sci. U.S.A. 91 (9), 3744-3748 (1994) PUBMED 8170981 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U86090.1. FEATURES Location/Qualifiers source 1..963 /organism="Mus musculus" /db_xref="taxon:10090" /chromosome="18" /map="18 1.0 cM" Protein 1..963 /product="kinesin family member 5B" /function="motor protein" /note="kinesin heavy chain member 5B, ubiquitous" Region 8..323 /region_name="Kinesin motor, catalytic domain" /note="KISc" /db_xref="CDD:14817" Region <422..908 /region_name="HOOK protein" /note="HOOK" /db_xref="CDD:23846" Region 678..907 /region_name="Intermediate filament protein" /note="Filament" /db_xref="CDD:16593" CDS 1..963 /gene="Kif5b" /coded_by="NM_008448.1:245..3136" /note="go_component: kinesin complex [goid 0005871] [evidence TAS] [pmid 10964943]; go_component: microtubule associated complex [goid 0005875] [evidence IEA]; go_function: ATP binding [goid 0005524] [evidence IEA]; go_function: motor activity [goid 0003774] [evidence IEA]; go_function: protein binding [goid 0005515] [evidence IPI] [pmid 10964943]; go_function: microtubule motor activity [goid 0003777] [evidence IEA]; go_function: microtubule motor activity [goid 0003777] [evidence TAS] [pmid 10964943]; go_process: mitochondrial transport [goid 0006839] [evidence TAS] [pmid 11486041]; go_process: microtubule-based process [goid 0007017] [evidence TAS] [pmid 11486041]; go_process: cytoplasm organization and biogenesis [goid 0007028] [evidence IMP] [pmid 9657148]" /db_xref="GeneID:16573" /db_xref="LocusID:16573" /db_xref="MGI:1098268"