Match to: gi|33468887 Score: 242 capping protein (actin filament) muscle Z-line, alpha 1; capping protein alpha 1 [Mus musculus] Found in search of C:\Xcalibur\sequest\059_B071504_14.txt Nominal mass (Mr): 32940; Calculated pI value: 5.34 NCBI BLAST search of gi|33468887 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Links to retrieve other entries containing this sequence from NCBI Entrez: gi|34223730 from Mus musculus gi|16740716 from Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 16% Matched peptides shown in Bold Red 1 MADFEDRVSD EEKVRIAAKF ITHAPPGEFN EVFNDVRLLL NNDNLLREGA 51 AHAFAQYNMD QFTPVKIEGY DDQVLITEHG DLGNSRFLDP RNQISFKFDH 101 LRKEASDPQP EDVDGGLKSW RESCDSALRA YVKDHYSNGF CTVYAKTIDG 151 QQTIIACIES HQFQPKNFWN GRWRSEWKFT ITPPSAQVVG VLKIQVHYYE 201 DGNVQLVSHK DVQDSVTVSN EVQTTKEFIK IIESAENEYQ TAISENYQTM 251 SDTTFKALRR QLPVTRTKID WNKILSYKIG KEMQNA
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 38 - 47 599.61 1197.19 1197.40 -0.21 0 LLLNNDNLLR (Ions score 55) 92 - 97 736.82 735.81 735.84 -0.03 0 NQISFK (Ions score 31) 104 - 118 779.19 1556.37 1556.60 -0.23 0 EASDPQPEDVDGGLK (Ions score 74) 211 - 226 876.14 1750.27 1749.85 0.42 0 DVQDSVTVSNEVQTTK (Ions score 83) LOCUS NP_033927 286 aa linear ROD 23-JAN-2004 DEFINITION capping protein (actin filament) muscle Z-line, alpha 1; capping protein alpha 1 [Mus musculus]. ACCESSION NP_033927 VERSION NP_033927.1 GI:33468887 DBSOURCE REFSEQ: accession NM_009797.1 KEYWORDS . SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 286) AUTHORS Hart,M.C., Korshunova,Y.O. and Cooper,J.A. TITLE Vertebrates have conserved capping protein alpha isoforms with specific expression patterns JOURNAL Cell Motil. Cytoskeleton 38 (2), 120-132 (1997) PUBMED 9331217 COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC016232.1 and U16740.1. FEATURES Location/Qualifiers source 1..286 /organism="Mus musculus" /strain="FVB/N" /db_xref="taxon:10090" /chromosome="3" /map="3 55.0 cM" Protein 1..286 /product="capping protein (actin filament) muscle Z-line, alpha 1" /note="capping protein alpha 1" Region 5..286 /region_name="F-actin capping protein, alpha subunit [Cytoskeleton]" /note="KOG0836" /db_xref="CDD:18629" CDS 1..286 /gene="Capza1" /coded_by="NM_009797.1:17..877" /note="go_component: F-actin capping protein complex [goid 0008290] [evidence IEA]; go_function: structural constituent of cytoskeleton [goid 0005200] [evidence IEA]; go_function: actin binding [goid 0003779] [evidence IEA]; go_function: F-actin capping activity [goid 0003782] [evidence IEA]; go_process: actin cytoskeleton organization and biogenesis [goid 0030036] [evidence IEA]" /db_xref="GeneID:12340" /db_xref="LocusID:12340" /db_xref="MGI:106227"