Match to: gi|13529464 Score: 183 Nucleolin [Mus musculus] Found in search of C:\Xcalibur\sequest\061_A080504_7.txt Nominal mass (Mr): 76779; Calculated pI value: 4.70 NCBI BLAST search of gi|13529464 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 5% Matched peptides shown in Bold Red 1 MVKLAKAGKT HGEAKKMAPP PKEVEEDSED EEMSEDEDDS SGEEEVVIPQ 51 KKGKKATTTP AKKVVVSQTK KAAVPTPAKK AAVTPGKKAV ATPAKKNITP 101 AKVIPTPGKK GAAQAKALVP TPGKKGAATP AKGAKNGKNA KKEDSDEDED 151 EEDEEDSDED EDDEEEDEFE PPIVKGVKPA KAAPAAPASE DEEDDEDEDD 201 EEEEDEEEED DSEEEVMEIT TAKGKKTPAK VVPMKAKSVA EEEDEEEEDE 251 DDEDEDDEEE DDEDDDEEEE EEEPVKAAPG KRKKEMTKQK EAPEAKKQKV 301 EGSEPTTPFN LFIGNLNPNK SVNELKFAIS ELFAKNDLAV VDVRTGTNRK 351 FGYVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK 401 NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE 451 KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK 501 ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE 551 GRTIRLELQG SNSRSQPSKT LFVKGLSEDT TEETLKESFE GSVRARIVTD 601 RETGSSKGFG FVDFNSEEDA KAAKEAMEDG EIDGNKVTLD WAKPKGEGGF 651 GGRGGGRGGF GGRGGGRGGR GGFGGRGRGG FGGRGGFRGG RGGGGDFKPQ 701 GKKTKFE
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 488 - 500 699.50 1396.99 1396.60 0.39 0 TLVLSNLSYSATK (Ions score 84) 501 - 509 562.82 1123.63 1123.23 0.41 0 ETLEEVFEK (Ions score 34) 608 - 621 781.86 1561.71 1561.62 0.08 0 GFGFVDFNSEEDAK (Ions score 65) LOCUS AAH05460 707 aa linear ROD 29-JUN-2004 DEFINITION Nucleolin [Mus musculus]. ACCESSION AAH05460 VERSION AAH05460.1 GI:13529464 DBSOURCE accession BC005460.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 707) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 707) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (27-MAR-2001) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov/ COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Gilbert Smith, Ph.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Sequencing Group at the Stanford Human Genome Center, Stanford University School of Medicine, Stanford, CA 94305 Web site: http://www-shgc.stanford.edu/ Contact: (Dickson, Mark) mcd@paxil.stanford.edu Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers, R. M. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov/ Series: IRAK Plate: 8 Row: d Column: 2 This clone was selected for full length sequencing because it passed the following selection criteria: matched mRNA gi: 7106376. Method: conceptual translation. FEATURES Location/Qualifiers source 1..707 /organism="Mus musculus" /strain="CZECH II" /db_xref="taxon:10090" /clone="MGC:6363 IMAGE:3495665" /tissue_type="Mammary tumor metastatized to lung. Tumor arose spontaneously from a senescent normal mammary (clonal) outgrowth infected with the virus MMTV." /clone_lib="NCI_CGAP_Lu29" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" Protein 1..707 /product="nucleolin" CDS 1..707 /gene="Ncl" /coded_by="BC005460.1:53..2176" /db_xref="LocusID:17975" /db_xref="MGI:97286"