Match to: gi|41945510 Score: 200 Dctn1 protein [Mus musculus] Found in search of C:\Xcalibur\sequest\060_B072704_1.txt Nominal mass (Mr): 139778; Calculated pI value: 5.53 NCBI BLAST search of gi|41945510 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 3% Matched peptides shown in Bold Red 1 MSTEASARPL RVGSRVEVIG KGHRGTVAYV GATLFATGKW VGVILDEAKG 51 KNDGTVQGRK YFTCDEGHGI FVRQSQIQVF EDGADTTSLE TPDSSASKVL 101 KREGADAAAK TSKLRGLKPK KAPTARKTTT RRPKPTRPAS TGVAGPSSSL 151 GPSGSASAGE LSSSEPSTPA QTPLAAPIIP TPALTSPGAA PPLPSPSKEE 201 EGLRAQVRDL EEKLETLRLK RSEDKAKLKE LEKHKIQLEQ VQEWKSKMQE 251 QQADLQRRLK EARKEAKEAL EAKERYMEEM ADTADAIEMA TLDKEMAEER 301 AESLQQEVEA LKERVDELTT DLEILKAEIE EKGSDGAASS YQLKQLEEQN 351 ARLKDALVRM RDLSSSEKQE HVKLQKLMEK KNQELEVVRQ QRERLQEELS 401 QAESTIDELK EQVDAALGAE EMVEMLTDRN LNLEEKVREL RETVGDLEAM 451 NEMNDELQEN ARETELELRE QLDMAGARVR EAQKRVEAAQ ETVADYQQTI 501 KKYRQLTAHL QDVNRELTNQ QEASVERQQQ PPPETFDFKI KFAETKAHAK 551 AIEMELRQME VAQANRHMSL LTAFMPDSFL RPGGDHDCVL VLLLMPRLIC 601 KAELIRKQAQ EKFDLSENCS ERPGLRGAAG EQLSFAAGLV YSLSLLQATL 651 HRYEHALSQC SVDVYKKVGS LYPEMSAHER SLDFLIELLH KDQLDETVNV 701 EPLTKAIKYY QHLYSIHLAE QPEDSTMQLA DHIKFTQSAL DCMGVEVGRL 751 RAFLQGGQEA TDIALLLRDL ETSCSDTRQF CKKIRRRMPG TDAPGIPAAL 801 AFGSQVSDTL LDCRKHLTWV VAVLQEVAAA AAQLIAPLAE NEGLPVAALE 851 ELAFKASEQI YGSPSSSPYE CLRQSCTILI STMNKLATAM QEGEYDAERP 901 PSKPPPVELR AAALRAEITD AEGLGLKLED RETVIKELKK SLKIKGEELS 951 EANVRLSLLE KKLDSAAKDA DERIEKVQTR LDETQTLLRK KEKDFEETMD 1001 ALQADIDQLE AEKAELKQRL NSQSKRTIEG LRGPPPSGIA TLVSGIAGEE 1051 PQRGGAPGQA PGALPGPGLV KDSPLLLQQI SAMRLHISQL QHENSILRGA 1101 QMKASLAALP PLHVAKLSLP PHEGPGGNLV AGALYRKTSQ LLEKLNQLST 1151 HTHVVDITRS SPAAKSPSAQ LMEQVAQLKS LSDTIEKLKD EVLKETVTQR 1201 PGATVPTDFA TFPSSAFLRA KEEQQDDTVY MGKVTFSCAA GLGQRHRLVL 1251 TQEQLHQLHS RLIS
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 516 - 527 703.00 1403.99 1403.47 0.52 0 ELTNQQEASVER (Ions score 43) 981 - 989 545.32 1088.63 1088.23 0.40 0 LDETQTLLR (Ions score 54) 1145 - 1159 868.20 1734.39 1733.95 0.45 0 LNQLSTHTHVVDITR (Ions score 61) 1166 - 1179 765.82 1529.62 1529.77 -0.15 0 SPSAQLMEQVAQLK (Ions score 60) LOCUS AAH66061 1264 aa linear ROD 24-FEB-2004 DEFINITION Dctn1 protein [Mus musculus]. ACCESSION AAH66061 VERSION AAH66061.1 GI:41945510 DBSOURCE accession BC066061.1 KEYWORDS MGC. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 1264) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (residues 1 to 1264) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (02-FEB-2004) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov/ COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: Dr. Jim Lin, University of Iowa cDNA Library Preparation: M. Bento Soares, University of Iowa cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: University of Iowa, Dr. M. Bento Soares and Dr. Thomas L. Casavant. Web site: http://genome.uiowa.edu/ Contact: bento-soares@uiowa.edu; tom-casavant@uiowa.edu Bonaldo,M.F., Akabogu,I., Bair,T., Bair,J., Crouch,K., Davis,A., Fishler,K., Keppel,C., Kucaba,T., Lebeck,M., Melo,A., Schaefer,K., Scheetz,T., Smith,C., Snir,E., Tack,D., Trout,K., Walters,J., Casavant,T., Soares,M.B. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov/ Series: Plate: Row: Column: 0. Method: conceptual translation. FEATURES Location/Qualifiers source 1..1264 /organism="Mus musculus" /strain="C57BL/6" /db_xref="taxon:10090" /clone="MGC:86152 IMAGE:6849060" /tissue_type="Brain, mouse, 13.5,14.5,16.5,17.5 dpc" /clone_lib="NIH_BMAP_FY0" /lab_host="DH10B" /note="Vector: pYX-ASC" Protein 1..1264 /product="Dctn1 protein" Region 12..78 /region_name="CAP-Gly domain. Cytoskeleton-associated proteins (CAPs) are involved in the organisation of microtubules and transportation of vesicles and organelles along the cytoskeletal network. A conserved motif, CAP-Gly, has been identified in a number of CAPs, including CLIP-170 and dynactins. The crystal structure of Caenorhabditis elegans F53F4.3 protein CAP-Gly domain was recently solved. The domain contains three beta-strands. The most conserved sequence, GKNDG, is located in two consecutive sharp turns on the surface, forming the entrance to a groove" /note="CAP_GLY" /db_xref="CDD:pfam01302" Region 299..561 /region_name="HOOK protein. This family consists of several HOOK1, 2 and 3 proteins from different eukaryotic organisms. The different members of the human gene family are HOOK1, HOOK2 and HOOK3. Different domains have been identified in the three human HOOK proteins, and it was demonstrated that the highly conserved NH2-domain mediates attachment to microtubules, whereas the central coiled-coil motif mediates homodimerisation and the more divergent C-terminal domains are involved in binding to specific organelles (organelle-binding domains). It has been demonstrated that endogenous HOOK3 binds to Golgi membranes, whereas both HOOK1 and HOOK2 are localised to discrete but unidentified cellular structures. In mice the Hook1 gene is predominantly expressed in the testis. Hook1 function is necessary for the correct positioning of microtubular structures within the haploid germ cell. Disruption of Hook1 function in mice causes abnormal sperm head shape and fragile attachment of the flagellum to the sperm head" /note="HOOK" /db_xref="CDD:pfam05622" CDS 1..1264 /gene="Dctn1" /coded_by="BC066061.1:63..3857" /db_xref="LocusID:13191" /db_xref="MGI:107745"