Match to: gi|50400412 Score: 568 Ubiquitin ligase protein DZIP3 (DAZ-interacting protein 3 homolog) Found in search of C:\Xcalibur\sequest\061_A080504_4.txt Nominal mass (Mr): 138021; Calculated pI value: 5.23 NCBI BLAST search of gi|50400412 against nr Unformatted sequence string for pasting into other applications Taxonomy: Mus musculus Variable modifications: Acetyl (N-term),Oxidation (M),Propionamide (C),Sulphone (M) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Sequence Coverage: 8% Matched peptides shown in Bold Red 1 MDSLAEEFFV SGNPDVEEQT KEETEIIAEK PVTQLDKQKM DISADPEPVN 51 ALLEIKKVLN PISALPKGVF PNIEKFIQED FSFQTMQREV TTHSQTGEEI 101 VPALTLHFLI TQLEMALRNI QASNYTAQQI NVGYYLTLLF LYGVALTERA 151 KKEDCIEAEN KFLVMKMVIQ ESEICENFMC LVYFGRGLLR CAQKRYNGAL 201 LEFYKSLQEI GDTDDNWFEV DPTDDEDLPT TFKDSLNNFI KTTESNIMKE 251 TICSYLDCER SCEADILKNT NYKGFFQLMC SKSCCIYFHK ICWKKFKNLK 301 YPGESDQSFS GQKCLKEGCP GDMVRMLQCD VPGIVKILFE VVRKDEYITI 351 ENLGASYKNL MSLELTDTDI RPKFNLKPNT KDEVPIFKLD YNYFYHLLHI 401 IIISGTDMVR QIFDEAMPPT LLKKELLIHK NVLEPYYNHL WTNHPLGGSW 451 HLLYPPNKEL PQSKQFDLCL LLALIKHLNV FPAPRKGWDM EPPSSDLSKS 501 ADILRLCKYR DILLSEILMN GLTELQFNSI WKKVSDILLR LGMKQDDLDK 551 VKENPIENIS LDYHQLSIYL GIPVPEIIQR MLSCYQQGIT LQSITGSQRL 601 DVEEFQNDEE DLSPPVMEYN IDVKSNTEIQ LAEINKDVAS IPSESSTESV 651 KDLQEVKSKT KKKKRTKSNK KDKDSEDEQV SYMVEKDDQL ETEQVDVNTL 701 STYMKTDTSD AQEDSAAEDK FCSLDELHIL DMVEQGSSGK ESTDFKETEK 751 ERLAHQHQLY KLQYECEDYK RQLKTVTFRW QENQMLIKKK EKIIVSLNQQ 801 VAFGINKMSK LQRQIHAKDD EIKNLKDQLS LKRSQWEMEK HNLESTVKTY 851 LNKLNAETSR ALTAEVYFLQ CRRDFGLLHL EQTEKECLNQ LARVTHMAAS 901 NLESLQLKAA VDSWNAIVAD VRNKIAFLRT QYNEQINKVK QGFALSTLPP 951 VQLPPPPPSP EILIQQFLGR PLVKESFFRP ILTVPQMPAV CPGVISAAVQ 1001 PRPPLMPGIT WAMPTPIGDT VSPSASLCSE PLMINWERIT DRLKTAFPQQ 1051 TRKELTDFLQ QLKDSHGKSV SRLTFDEIVY KISQMIEPKK SESEEKSAQD 1101 GNNASPSHTA SQPNAPQDPK SAQGSATWEG DKDMDNEEEE EEPCVICHEN 1151 LSPENLSVLP CAHKFHSQCI RPWLMQQGTC PTCRLHVLQP EEFPGHPNGQ 1201 LPKI
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence 76 - 88 839.29 1676.57 1676.86 -0.30 0 FIQEDFSFQTMQR (Ions score 72) 301 - 313 715.92 1429.81 1429.46 0.35 0 YPGESDQSFSGQK (Ions score 70) 344 - 358 872.99 1743.96 1743.93 0.03 1 KDEYITIENLGASYK (Ions score 74) 359 - 373 874.39 1746.76 1746.01 0.75 0 NLMSLELTDTDIRPK (Ions score 84) 741 - 752 750.26 1498.49 1498.57 -0.07 2 ESTDFKETEKER (Ions score 65) 780 - 788 595.91 1189.80 1189.40 0.41 0 WQENQMLIK (Ions score 35) 894 - 908 821.93 1641.84 1641.90 -0.07 0 VTHMAASNLESLQLK (Ions score 99) 1053 - 1063 682.46 1362.90 1362.59 0.31 1 KELTDFLQQLK (Ions score 49) 1054 - 1063 618.25 1234.49 1234.42 0.08 0 ELTDFLQQLK (Ions score 41) LOCUS Q7TPV2 1204 aa linear ROD 01-OCT-2004 DEFINITION Ubiquitin ligase protein DZIP3 (DAZ-interacting protein 3 homolog). ACCESSION Q7TPV2 VERSION Q7TPV2 GI:50400412 DBSOURCE swissprot: locus DZP3_MOUSE, accession Q7TPV2; class: standard. extra accessions:Q80TU4,Q8BYK7,created: Oct 1, 2004. sequence updated: Oct 1, 2004. annotation updated: Oct 1, 2004. xrefs: gi: 28972344, gi: 28972345, gi: 26333094, gi: 26333095, gi: 31807197, gi: 31807198, gi: 15713220, gi: 46863979, gi: 40069691 xrefs (non-sequence databases): MGI1917433, PROSITEPS00518, PROSITEPS50089 KEYWORDS Alternative splicing; Coiled coil; Ligase; Metal-binding; RNA-binding; Ubl conjugation pathway; Zinc; Zinc-finger. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. REFERENCE 1 (residues 1 to 1204) AUTHORS Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S., Nakajima,D., Nagase,T., Ohara,O. and Koga,H. TITLE Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries JOURNAL DNA Res. 10 (1), 35-48 (2003) MEDLINE 22579291 PUBMED 12693553 REMARK SEQUENCE FROM N.A. (ISOFORM 3). TISSUE=Brain REFERENCE 2 (residues 1 to 1204) AUTHORS Okazaki,Y., Furuno,M., Kasukawa,T., Adachi,J., Bono,H., Kondo,S., Nikaido,I., Osato,N., Saito,R., Suzuki,H., Yamanaka,I., Kiyosawa,H., Yagi,K., Tomaru,Y., Hasegawa,Y., Nogami,A., Schonbach,C., Gojobori,T., Baldarelli,R., Hill,D.P., Bult,C., Hume,D.A., Quackenbush,J., Schriml,L.M., Kanapin,A., Matsuda,H., Batalov,S., Beisel,K.W., Blake,J.A., Bradt,D., Brusic,V., Chothia,C., Corbani,L.E., Cousins,S., Dalla,E., Dragani,T.A., Fletcher,C.F., Forrest,A., Frazer,K.S., Gaasterland,T., Gariboldi,M., Gissi,C., Godzik,A., Gough,J., Grimmond,S., Gustincich,S., Hirokawa,N., Jackson,I.J., Jarvis,E.D., Kanai,A., Kawaji,H., Kawasawa,Y., Kedzierski,R.M., King,B.L., Konagaya,A., Kurochkin,I.V., Lee,Y., Lenhard,B., Lyons,P.A., Maglott,D.R., Maltais,L., Marchionni,L., McKenzie,L., Miki,H., Nagashima,T., Numata,K., Okido,T., Pavan,W.J., Pertea,G., Pesole,G., Petrovsky,N., Pillai,R., Pontius,J.U., Qi,D., Ramachandran,S., Ravasi,T., Reed,J.C., Reed,D.J., Reid,J., Ring,B.Z., Ringwald,M., Sandelin,A., Schneider,C., Semple,C.A., Setou,M., Shimada,K., Sultana,R., Takenaka,Y., Taylor,M.S., Teasdale,R.D., Tomita,M., Verardo,R., Wagner,L., Wahlestedt,C., Wang,Y., Watanabe,Y., Wells,C., Wilming,L.G., Wynshaw-Boris,A., Yanagisawa,M., Yang,I., Yang,L., Yuan,Z., Zavolan,M., Zhu,Y., Zimmer,A., Carninci,P., Hayatsu,N., Hirozane-Kishikawa,T., Konno,H., Nakamura,M., Sakazume,N., Sato,K., Shiraki,T., Waki,K., Kawai,J., Aizawa,K., Arakawa,T., Fukuda,S., Hara,A., Hashizume,W., Imotani,K., Ishii,Y., Itoh,M., Kagawa,I., Miyazaki,A., Sakai,K., Sasaki,D., Shibata,K., Shinagawa,A., Yasunishi,A., Yoshino,M., Waterston,R., Lander,E.S., Rogers,J., Birney,E. and Hayashizaki,Y. TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420 (6915), 563-573 (2002) MEDLINE 22354683 PUBMED 12466851 REMARK SEQUENCE OF 1-787 FROM N.A. (ISOFORM 1). STRAIN=C57BL/6J; TISSUE=Hypothalamus REFERENCE 3 (residues 1 to 1204) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) MEDLINE 22388257 PUBMED 12477932 REMARK SEQUENCE OF 1-664 FROM N.A. (ISOFORM 2). STRAIN=C3H/He; TISSUE=Osteoblast REFERENCE 4 (residues 1 to 1204) AUTHORS Poux,S. JOURNAL Unpublished REMARK CONCEPTUAL TRANSLATION OF 788-1204. REFERENCE 5 (residues 1 to 1204) AUTHORS Strausberg,R.L. TITLE Direct Submission JOURNAL Submitted (~APR-2004) REMARK SEQUENCE OF 767-1133 FROM N.A. (ISOFORM 1). TISSUE=Head, and Retina REFERENCE 6 (residues 1 to 1204) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Direct Submission JOURNAL Submitted (~DEC-2003) REMARK SEQUENCE OF 1062-1204 FROM N.A. (ISOFORM 1). COMMENT ------------------------------------------------------------------- This SWISS-PROT entry is copyright. It is produced through a collaboration between the Swiss Institute of Bioinformatics and the EMBL outstation - the European Bioinformatics Institute. The original entry is available from http://www.expasy.ch/sprot and http://www.ebi.ac.uk/sprot ------------------------------------------------------------------. [FUNCTION] E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA (By similarity). [PATHWAY] Ubiquitin conjugation; third step. [SUBUNIT] Probably interacts with DAZL (By similarity). [SUBCELLULAR LOCATION] Cytoplasmic (By similarity). [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q7TPV2-1; Sequence=Displayed; Name=2; IsoId=Q7TPV2-2; Sequence=VSP_010977; Note=No experimental confirmation available; Name=3; IsoId=Q7TPV2-3; Sequence=VSP_010973, VSP_010974, VSP_010975, VSP_010976; Note=No experimental confirmation available. [SIMILARITY] Contains 1 RING-type zinc finger. [CAUTION] The region from Lys-788 to Ile-1204 is deduced from EST sequences by similarity to the human sequence. FEATURES Location/Qualifiers source 1..1204 /organism="Mus musculus" /db_xref="taxon:10090" gene 1..1204 /gene="Name=Dzip3" /note="synonym: Synonyms=Kiaa0675" Protein 1..1204 /gene="Name=Dzip3" /product="Ubiquitin ligase protein DZIP3" /EC_number="6.3.2.-" Region 1..889 /gene="Name=Dzip3" /region_name="Splicing variant" /note="Missing (in isoform 3). /FTId=VSP_010973." Region 306 /gene="Name=Dzip3" /region_name="Conflict" /note="D -> G (in Ref. 3)." Region 382..587 /gene="Name=Dzip3" /region_name="Splicing variant" /note="Missing (in isoform 2). /FTId=VSP_010977." Region 650 /gene="Name=Dzip3" /region_name="Conflict" /note="V -> I (in Ref. 3)." Region 657..664 /gene="Name=Dzip3" /region_name="Domain" /note="Poly-Lys." Region 746..861 /gene="Name=Dzip3" /region_name="Domain" /note="Coiled coil (Potential)." Region 890..899 /gene="Name=Dzip3" /region_name="Splicing variant" /note="QLARVTHMAA -> MFPLCLLFYI (in isoform 3). /FTId=VSP_010974." Region 906..941 /gene="Name=Dzip3" /region_name="Domain" /note="Coiled coil (Potential)." Region 954..960 /gene="Name=Dzip3" /region_name="Domain" /note="Poly-Pro." Region 1135..1153 /gene="Name=Dzip3" /region_name="Splicing variant" /note="DNEEEEEEPCVICHENLSP -> VRPNLLTVNTFRSERKRM V (in isoform 3). /FTId=VSP_010975." Region 1137..1142 /gene="Name=Dzip3" /region_name="Domain" /note="Poly-Glu." Region 1144..1184 /gene="Name=Dzip3" /region_name="Zinc finger region" /note="RING-type (atypical)." Region 1154..1204 /gene="Name=Dzip3" /region_name="Splicing variant" /note="Missing (in isoform 3). /FTId=VSP_010976."