ERX2070735	SAMEA104128455	NextSeq_500_paired_end_sequencing;_ChIP-seq_of_Mediator_subunits_in_Mediator_mutants_and_wild_type_yeast	ChIP-Seq	GENOMIC	ChIP	1	Application_Read	Reverse	35	NextSeq_500	ERA961767	ERS1792348	ERP023597	PRJEB21355	2017-12-09T23:22:12Z	Organism taxonomy_id="4932" taxonomy_name="Saccharomyces cerevisiae"	Alias=E-MTAB-5824:med2 med3 med15 kin28-AA plus rapa med17-myc	Broker name=ArrayExpress	Description=Protocols: Yeast were grown at 30˚C in YPD to A600 of 0.8; rapamycin added to 1 microgram/ml and incubated 1 hr additional ChIP performed by standard methods. Antibody incubation was overnight at 4 C. The NEB Next Ultra II DNA library prep kit was used according to manufacturer's instructions.	ENA checklist=ERC000011	INSDC center alias=Wadsworth Center, NY State Dept. of Health	INSDC center name=Wadsworth Center, NY State Dept. of Health	INSDC first public=2017-12-01T17:01:52Z	INSDC last update=2017-06-19T18:10:33Z	INSDC status=public	SRA accession=ERS1792348	Sample Name=ERS1792348	Title=med2 med3 med15 kin28-AA plus rapa med17-myc	genetic modification=targeted mutation	genotype=Mat a med3::KanMX med15::KanMX med2::KanMX leu20 met150 ura30 trp1- tor1-1 fpr1::NAT RPL13A-2XFKBP::TRP1 KIN28-FRB::hph srb4-13myc::URA3	growth condition=YEPD	organism=Saccharomyces cerevisiae