Job ID = 14521633 SRX = SRX9399015 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 5656592 spots for SRR12935297/SRR12935297.sra Written 5656592 spots for SRR12935297/SRR12935297.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:37 5656592 reads; of these: 5656592 (100.00%) were paired; of these: 991544 (17.53%) aligned concordantly 0 times 3915613 (69.22%) aligned concordantly exactly 1 time 749435 (13.25%) aligned concordantly >1 times ---- 991544 pairs aligned concordantly 0 times; of these: 189705 (19.13%) aligned discordantly 1 time ---- 801839 pairs aligned 0 times concordantly or discordantly; of these: 1603678 mates make up the pairs; of these: 1262451 (78.72%) aligned 0 times 231981 (14.47%) aligned exactly 1 time 109246 (6.81%) aligned >1 times 88.84% overall alignment rate Time searching: 00:02:37 Overall time: 00:02:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 527096 / 4748107 = 0.1110 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:21:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:21:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:21:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:21:04: 1000000 INFO @ Sat, 15 Jan 2022 21:21:09: 2000000 INFO @ Sat, 15 Jan 2022 21:21:13: 3000000 INFO @ Sat, 15 Jan 2022 21:21:18: 4000000 INFO @ Sat, 15 Jan 2022 21:21:22: 5000000 INFO @ Sat, 15 Jan 2022 21:21:27: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:21:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:21:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:21:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:21:31: 7000000 INFO @ Sat, 15 Jan 2022 21:21:35: 1000000 INFO @ Sat, 15 Jan 2022 21:21:37: 8000000 INFO @ Sat, 15 Jan 2022 21:21:40: 2000000 INFO @ Sat, 15 Jan 2022 21:21:42: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 21:21:42: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 21:21:42: #1 total tags in treatment: 4140520 INFO @ Sat, 15 Jan 2022 21:21:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:21:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:21:42: #1 tags after filtering in treatment: 2877365 INFO @ Sat, 15 Jan 2022 21:21:42: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:21:42: #1 finished! INFO @ Sat, 15 Jan 2022 21:21:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:21:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:21:42: #2 number of paired peaks: 23 WARNING @ Sat, 15 Jan 2022 21:21:42: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:21:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:21:45: 3000000 INFO @ Sat, 15 Jan 2022 21:21:50: 4000000 INFO @ Sat, 15 Jan 2022 21:21:55: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:22:00: 6000000 INFO @ Sat, 15 Jan 2022 21:22:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:22:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:22:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:22:05: 7000000 INFO @ Sat, 15 Jan 2022 21:22:05: 1000000 INFO @ Sat, 15 Jan 2022 21:22:10: 8000000 INFO @ Sat, 15 Jan 2022 21:22:11: 2000000 INFO @ Sat, 15 Jan 2022 21:22:16: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 21:22:16: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 21:22:16: #1 total tags in treatment: 4140520 INFO @ Sat, 15 Jan 2022 21:22:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:22:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:22:16: #1 tags after filtering in treatment: 2877365 INFO @ Sat, 15 Jan 2022 21:22:16: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:22:16: #1 finished! INFO @ Sat, 15 Jan 2022 21:22:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:22:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:22:16: #2 number of paired peaks: 23 WARNING @ Sat, 15 Jan 2022 21:22:16: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:22:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.10_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 21:22:16: 3000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:22:21: 4000000 INFO @ Sat, 15 Jan 2022 21:22:26: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:22:32: 6000000 INFO @ Sat, 15 Jan 2022 21:22:36: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:22:41: 8000000 INFO @ Sat, 15 Jan 2022 21:22:46: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 21:22:46: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 21:22:46: #1 total tags in treatment: 4140520 INFO @ Sat, 15 Jan 2022 21:22:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:22:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:22:46: #1 tags after filtering in treatment: 2877365 INFO @ Sat, 15 Jan 2022 21:22:46: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:22:46: #1 finished! INFO @ Sat, 15 Jan 2022 21:22:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:22:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:22:46: #2 number of paired peaks: 23 WARNING @ Sat, 15 Jan 2022 21:22:46: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:22:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399015/SRX9399015.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling