Job ID = 14520628 SRX = SRX9349774 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8663270 spots for SRR12883918/SRR12883918.sra Written 8663270 spots for SRR12883918/SRR12883918.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:17 8663270 reads; of these: 8663270 (100.00%) were paired; of these: 622143 (7.18%) aligned concordantly 0 times 7526918 (86.88%) aligned concordantly exactly 1 time 514209 (5.94%) aligned concordantly >1 times ---- 622143 pairs aligned concordantly 0 times; of these: 330548 (53.13%) aligned discordantly 1 time ---- 291595 pairs aligned 0 times concordantly or discordantly; of these: 583190 mates make up the pairs; of these: 458057 (78.54%) aligned 0 times 76572 (13.13%) aligned exactly 1 time 48561 (8.33%) aligned >1 times 97.36% overall alignment rate Time searching: 00:08:17 Overall time: 00:08:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 707436 / 8315547 = 0.0851 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:50: 1000000 INFO @ Sat, 15 Jan 2022 19:49:56: 2000000 INFO @ Sat, 15 Jan 2022 19:50:02: 3000000 INFO @ Sat, 15 Jan 2022 19:50:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:15: 5000000 INFO @ Sat, 15 Jan 2022 19:50:20: 1000000 INFO @ Sat, 15 Jan 2022 19:50:22: 6000000 INFO @ Sat, 15 Jan 2022 19:50:27: 2000000 INFO @ Sat, 15 Jan 2022 19:50:28: 7000000 INFO @ Sat, 15 Jan 2022 19:50:34: 3000000 INFO @ Sat, 15 Jan 2022 19:50:35: 8000000 INFO @ Sat, 15 Jan 2022 19:50:40: 4000000 INFO @ Sat, 15 Jan 2022 19:50:41: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:47: 5000000 INFO @ Sat, 15 Jan 2022 19:50:48: 10000000 INFO @ Sat, 15 Jan 2022 19:50:49: 1000000 INFO @ Sat, 15 Jan 2022 19:50:54: 6000000 INFO @ Sat, 15 Jan 2022 19:50:55: 11000000 INFO @ Sat, 15 Jan 2022 19:50:55: 2000000 INFO @ Sat, 15 Jan 2022 19:51:00: 3000000 INFO @ Sat, 15 Jan 2022 19:51:01: 7000000 INFO @ Sat, 15 Jan 2022 19:51:01: 12000000 INFO @ Sat, 15 Jan 2022 19:51:06: 4000000 INFO @ Sat, 15 Jan 2022 19:51:07: 8000000 INFO @ Sat, 15 Jan 2022 19:51:08: 13000000 INFO @ Sat, 15 Jan 2022 19:51:11: 5000000 INFO @ Sat, 15 Jan 2022 19:51:14: 9000000 INFO @ Sat, 15 Jan 2022 19:51:14: 14000000 INFO @ Sat, 15 Jan 2022 19:51:17: 6000000 INFO @ Sat, 15 Jan 2022 19:51:21: 10000000 INFO @ Sat, 15 Jan 2022 19:51:21: 15000000 INFO @ Sat, 15 Jan 2022 19:51:22: 7000000 INFO @ Sat, 15 Jan 2022 19:51:24: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:51:24: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:51:24: #1 total tags in treatment: 7343700 INFO @ Sat, 15 Jan 2022 19:51:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:24: #1 tags after filtering in treatment: 4454450 INFO @ Sat, 15 Jan 2022 19:51:24: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:51:24: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:51:27: 11000000 INFO @ Sat, 15 Jan 2022 19:51:28: 8000000 INFO @ Sat, 15 Jan 2022 19:51:33: 9000000 INFO @ Sat, 15 Jan 2022 19:51:34: 12000000 INFO @ Sat, 15 Jan 2022 19:51:39: 10000000 INFO @ Sat, 15 Jan 2022 19:51:41: 13000000 INFO @ Sat, 15 Jan 2022 19:51:44: 11000000 INFO @ Sat, 15 Jan 2022 19:51:48: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:51:50: 12000000 INFO @ Sat, 15 Jan 2022 19:51:54: 15000000 INFO @ Sat, 15 Jan 2022 19:51:55: 13000000 INFO @ Sat, 15 Jan 2022 19:51:57: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:51:57: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:51:57: #1 total tags in treatment: 7343700 INFO @ Sat, 15 Jan 2022 19:51:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:57: #1 tags after filtering in treatment: 4454450 INFO @ Sat, 15 Jan 2022 19:51:57: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:51:57: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:52:01: 14000000 INFO @ Sat, 15 Jan 2022 19:52:06: 15000000 INFO @ Sat, 15 Jan 2022 19:52:08: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:52:08: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:52:08: #1 total tags in treatment: 7343700 INFO @ Sat, 15 Jan 2022 19:52:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:52:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:52:08: #1 tags after filtering in treatment: 4454450 INFO @ Sat, 15 Jan 2022 19:52:08: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:52:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:52:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:52:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:52:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:52:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:52:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349774/SRX9349774.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling