Job ID = 14521511 SRX = SRX8833559 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32143871 spots for SRR12333595/SRR12333595.sra Written 32143871 spots for SRR12333595/SRR12333595.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:44 32143871 reads; of these: 32143871 (100.00%) were unpaired; of these: 1127862 (3.51%) aligned 0 times 27963670 (87.00%) aligned exactly 1 time 3052339 (9.50%) aligned >1 times 96.49% overall alignment rate Time searching: 00:04:44 Overall time: 00:04:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18072717 / 31016009 = 0.5827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:17:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:17:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:17:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:17:45: 1000000 INFO @ Sat, 15 Jan 2022 21:17:50: 2000000 INFO @ Sat, 15 Jan 2022 21:17:55: 3000000 INFO @ Sat, 15 Jan 2022 21:18:00: 4000000 INFO @ Sat, 15 Jan 2022 21:18:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:18:09: 6000000 INFO @ Sat, 15 Jan 2022 21:18:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:18:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:18:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:18:14: 7000000 INFO @ Sat, 15 Jan 2022 21:18:15: 1000000 INFO @ Sat, 15 Jan 2022 21:18:19: 8000000 INFO @ Sat, 15 Jan 2022 21:18:20: 2000000 INFO @ Sat, 15 Jan 2022 21:18:23: 9000000 INFO @ Sat, 15 Jan 2022 21:18:25: 3000000 INFO @ Sat, 15 Jan 2022 21:18:28: 10000000 INFO @ Sat, 15 Jan 2022 21:18:30: 4000000 INFO @ Sat, 15 Jan 2022 21:18:32: 11000000 INFO @ Sat, 15 Jan 2022 21:18:35: 5000000 INFO @ Sat, 15 Jan 2022 21:18:38: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:18:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:18:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:18:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:18:40: 6000000 INFO @ Sat, 15 Jan 2022 21:18:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:18:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:18:43: #1 total tags in treatment: 12943292 INFO @ Sat, 15 Jan 2022 21:18:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:18:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:18:43: #1 tags after filtering in treatment: 12943292 INFO @ Sat, 15 Jan 2022 21:18:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:18:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:18:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:18:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:18:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:18:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:18:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 21:18:45: 7000000 INFO @ Sat, 15 Jan 2022 21:18:45: 1000000 pass1 - making usageList (0 chroms): 1161 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:18:49: 8000000 INFO @ Sat, 15 Jan 2022 21:18:50: 2000000 INFO @ Sat, 15 Jan 2022 21:18:54: 9000000 INFO @ Sat, 15 Jan 2022 21:18:55: 3000000 INFO @ Sat, 15 Jan 2022 21:18:58: 10000000 INFO @ Sat, 15 Jan 2022 21:19:00: 4000000 INFO @ Sat, 15 Jan 2022 21:19:03: 11000000 INFO @ Sat, 15 Jan 2022 21:19:05: 5000000 INFO @ Sat, 15 Jan 2022 21:19:08: 12000000 INFO @ Sat, 15 Jan 2022 21:19:10: 6000000 INFO @ Sat, 15 Jan 2022 21:19:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:19:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:19:13: #1 total tags in treatment: 12943292 INFO @ Sat, 15 Jan 2022 21:19:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:19:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:19:13: #1 tags after filtering in treatment: 12943292 INFO @ Sat, 15 Jan 2022 21:19:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:19:13: #1 finished! INFO @ Sat, 15 Jan 2022 21:19:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:19:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:19:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:19:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:19:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:19:14: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:19:19: 8000000 INFO @ Sat, 15 Jan 2022 21:19:23: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:19:27: 10000000 INFO @ Sat, 15 Jan 2022 21:19:32: 11000000 INFO @ Sat, 15 Jan 2022 21:19:37: 12000000 INFO @ Sat, 15 Jan 2022 21:19:42: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:19:42: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:19:42: #1 total tags in treatment: 12943292 INFO @ Sat, 15 Jan 2022 21:19:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:19:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:19:42: #1 tags after filtering in treatment: 12943292 INFO @ Sat, 15 Jan 2022 21:19:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:19:42: #1 finished! INFO @ Sat, 15 Jan 2022 21:19:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:19:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:19:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:19:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:19:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833559/SRX8833559.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling