Job ID = 14521510 SRX = SRX8833558 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 26650540 spots for SRR12333594/SRR12333594.sra Written 26650540 spots for SRR12333594/SRR12333594.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:48 26650540 reads; of these: 26650540 (100.00%) were unpaired; of these: 1554999 (5.83%) aligned 0 times 22558651 (84.65%) aligned exactly 1 time 2536890 (9.52%) aligned >1 times 94.17% overall alignment rate Time searching: 00:03:48 Overall time: 00:03:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14337798 / 25095541 = 0.5713 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:34: 1000000 INFO @ Sat, 15 Jan 2022 21:14:39: 2000000 INFO @ Sat, 15 Jan 2022 21:14:44: 3000000 INFO @ Sat, 15 Jan 2022 21:14:49: 4000000 INFO @ Sat, 15 Jan 2022 21:14:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:59: 6000000 INFO @ Sat, 15 Jan 2022 21:15:04: 1000000 INFO @ Sat, 15 Jan 2022 21:15:05: 7000000 INFO @ Sat, 15 Jan 2022 21:15:09: 2000000 INFO @ Sat, 15 Jan 2022 21:15:10: 8000000 INFO @ Sat, 15 Jan 2022 21:15:15: 3000000 INFO @ Sat, 15 Jan 2022 21:15:16: 9000000 INFO @ Sat, 15 Jan 2022 21:15:20: 4000000 INFO @ Sat, 15 Jan 2022 21:15:21: 10000000 INFO @ Sat, 15 Jan 2022 21:15:25: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:15:25: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:15:25: #1 total tags in treatment: 10757743 INFO @ Sat, 15 Jan 2022 21:15:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:25: 5000000 INFO @ Sat, 15 Jan 2022 21:15:25: #1 tags after filtering in treatment: 10757743 INFO @ Sat, 15 Jan 2022 21:15:25: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:15:25: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:15:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:15:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:15:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:15:31: 6000000 INFO @ Sat, 15 Jan 2022 21:15:34: 1000000 INFO @ Sat, 15 Jan 2022 21:15:36: 7000000 INFO @ Sat, 15 Jan 2022 21:15:39: 2000000 INFO @ Sat, 15 Jan 2022 21:15:41: 8000000 INFO @ Sat, 15 Jan 2022 21:15:45: 3000000 INFO @ Sat, 15 Jan 2022 21:15:47: 9000000 INFO @ Sat, 15 Jan 2022 21:15:50: 4000000 INFO @ Sat, 15 Jan 2022 21:15:52: 10000000 INFO @ Sat, 15 Jan 2022 21:15:56: 5000000 INFO @ Sat, 15 Jan 2022 21:15:56: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:15:56: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:15:56: #1 total tags in treatment: 10757743 INFO @ Sat, 15 Jan 2022 21:15:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:56: #1 tags after filtering in treatment: 10757743 INFO @ Sat, 15 Jan 2022 21:15:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:15:56: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:16:01: 6000000 INFO @ Sat, 15 Jan 2022 21:16:06: 7000000 INFO @ Sat, 15 Jan 2022 21:16:11: 8000000 INFO @ Sat, 15 Jan 2022 21:16:17: 9000000 INFO @ Sat, 15 Jan 2022 21:16:22: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:16:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:16:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:16:26: #1 total tags in treatment: 10757743 INFO @ Sat, 15 Jan 2022 21:16:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:26: #1 tags after filtering in treatment: 10757743 INFO @ Sat, 15 Jan 2022 21:16:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:16:26: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:16:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833558/SRX8833558.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling