Job ID = 14521507 SRX = SRX8833555 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15325281 spots for SRR12333591/SRR12333591.sra Written 15325281 spots for SRR12333591/SRR12333591.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:38 15325281 reads; of these: 15325281 (100.00%) were unpaired; of these: 459 (0.00%) aligned 0 times 13299664 (86.78%) aligned exactly 1 time 2025158 (13.21%) aligned >1 times 100.00% overall alignment rate Time searching: 00:01:38 Overall time: 00:01:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7068642 / 15324822 = 0.4613 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:10:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:10:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:10:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:10:41: 1000000 INFO @ Sat, 15 Jan 2022 21:10:48: 2000000 INFO @ Sat, 15 Jan 2022 21:10:53: 3000000 INFO @ Sat, 15 Jan 2022 21:10:58: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:05: 5000000 INFO @ Sat, 15 Jan 2022 21:11:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:11: 6000000 INFO @ Sat, 15 Jan 2022 21:11:12: 1000000 INFO @ Sat, 15 Jan 2022 21:11:18: 7000000 INFO @ Sat, 15 Jan 2022 21:11:18: 2000000 INFO @ Sat, 15 Jan 2022 21:11:24: 8000000 INFO @ Sat, 15 Jan 2022 21:11:25: 3000000 INFO @ Sat, 15 Jan 2022 21:11:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:11:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:11:26: #1 total tags in treatment: 8256180 INFO @ Sat, 15 Jan 2022 21:11:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:11:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:11:26: #1 tags after filtering in treatment: 8256180 INFO @ Sat, 15 Jan 2022 21:11:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:11:26: #1 finished! INFO @ Sat, 15 Jan 2022 21:11:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:11:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:11:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:11:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:11:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:11:32: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:11:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:11:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:11:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:11:38: 5000000 INFO @ Sat, 15 Jan 2022 21:11:41: 1000000 INFO @ Sat, 15 Jan 2022 21:11:45: 6000000 INFO @ Sat, 15 Jan 2022 21:11:48: 2000000 INFO @ Sat, 15 Jan 2022 21:11:52: 7000000 INFO @ Sat, 15 Jan 2022 21:11:54: 3000000 INFO @ Sat, 15 Jan 2022 21:11:58: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:12:00: 4000000 INFO @ Sat, 15 Jan 2022 21:12:00: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:12:00: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:12:00: #1 total tags in treatment: 8256180 INFO @ Sat, 15 Jan 2022 21:12:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:00: #1 tags after filtering in treatment: 8256180 INFO @ Sat, 15 Jan 2022 21:12:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:12:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:12:06: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:12:11: 6000000 INFO @ Sat, 15 Jan 2022 21:12:17: 7000000 INFO @ Sat, 15 Jan 2022 21:12:22: 8000000 INFO @ Sat, 15 Jan 2022 21:12:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:12:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:12:23: #1 total tags in treatment: 8256180 INFO @ Sat, 15 Jan 2022 21:12:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:12:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:12:23: #1 tags after filtering in treatment: 8256180 INFO @ Sat, 15 Jan 2022 21:12:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:12:23: #1 finished! INFO @ Sat, 15 Jan 2022 21:12:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:12:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:12:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:12:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:12:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833555/SRX8833555.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling