Job ID = 14521506 SRX = SRX8833554 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39732054 spots for SRR12333590/SRR12333590.sra Written 39732054 spots for SRR12333590/SRR12333590.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:48 39732054 reads; of these: 39732054 (100.00%) were unpaired; of these: 6892976 (17.35%) aligned 0 times 14734767 (37.09%) aligned exactly 1 time 18104311 (45.57%) aligned >1 times 82.65% overall alignment rate Time searching: 00:05:48 Overall time: 00:05:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 24165959 / 32839078 = 0.7359 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:21:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:21:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:21:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:21:48: 1000000 INFO @ Sat, 15 Jan 2022 21:21:56: 2000000 INFO @ Sat, 15 Jan 2022 21:22:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:22:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:22:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:22:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:22:13: 4000000 INFO @ Sat, 15 Jan 2022 21:22:20: 1000000 INFO @ Sat, 15 Jan 2022 21:22:21: 5000000 INFO @ Sat, 15 Jan 2022 21:22:28: 2000000 INFO @ Sat, 15 Jan 2022 21:22:30: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:22:37: 3000000 INFO @ Sat, 15 Jan 2022 21:22:39: 7000000 INFO @ Sat, 15 Jan 2022 21:22:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:22:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:22:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:22:46: 4000000 INFO @ Sat, 15 Jan 2022 21:22:47: 8000000 INFO @ Sat, 15 Jan 2022 21:22:48: 1000000 INFO @ Sat, 15 Jan 2022 21:22:53: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:22:53: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:22:53: #1 total tags in treatment: 8673119 INFO @ Sat, 15 Jan 2022 21:22:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:22:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:22:53: #1 tags after filtering in treatment: 8673119 INFO @ Sat, 15 Jan 2022 21:22:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:22:53: #1 finished! INFO @ Sat, 15 Jan 2022 21:22:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:22:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:22:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:22:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:22:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1381 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:22:56: 5000000 INFO @ Sat, 15 Jan 2022 21:22:57: 2000000 INFO @ Sat, 15 Jan 2022 21:23:04: 6000000 INFO @ Sat, 15 Jan 2022 21:23:05: 3000000 INFO @ Sat, 15 Jan 2022 21:23:12: 7000000 INFO @ Sat, 15 Jan 2022 21:23:14: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:23:21: 8000000 INFO @ Sat, 15 Jan 2022 21:23:22: 5000000 INFO @ Sat, 15 Jan 2022 21:23:26: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:23:26: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:23:26: #1 total tags in treatment: 8673119 INFO @ Sat, 15 Jan 2022 21:23:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:23:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:23:26: #1 tags after filtering in treatment: 8673119 INFO @ Sat, 15 Jan 2022 21:23:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:23:26: #1 finished! INFO @ Sat, 15 Jan 2022 21:23:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:23:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:23:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:23:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:23:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:23:30: 6000000 INFO @ Sat, 15 Jan 2022 21:23:38: 7000000 INFO @ Sat, 15 Jan 2022 21:23:46: 8000000 INFO @ Sat, 15 Jan 2022 21:23:52: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:23:52: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:23:52: #1 total tags in treatment: 8673119 INFO @ Sat, 15 Jan 2022 21:23:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:23:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:23:52: #1 tags after filtering in treatment: 8673119 INFO @ Sat, 15 Jan 2022 21:23:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:23:52: #1 finished! INFO @ Sat, 15 Jan 2022 21:23:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:23:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:23:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:23:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:23:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833554/SRX8833554.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling