Job ID = 14521472 SRX = SRX8833542 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 44871796 spots for SRR12333578/SRR12333578.sra Written 44871796 spots for SRR12333578/SRR12333578.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:37 44871796 reads; of these: 44871796 (100.00%) were unpaired; of these: 2424484 (5.40%) aligned 0 times 37983651 (84.65%) aligned exactly 1 time 4463661 (9.95%) aligned >1 times 94.60% overall alignment rate Time searching: 00:06:37 Overall time: 00:06:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 28595953 / 42447312 = 0.6737 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:19:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:19:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:19:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:19:35: 1000000 INFO @ Sat, 15 Jan 2022 21:19:40: 2000000 INFO @ Sat, 15 Jan 2022 21:19:44: 3000000 INFO @ Sat, 15 Jan 2022 21:19:49: 4000000 INFO @ Sat, 15 Jan 2022 21:19:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:19:59: 6000000 INFO @ Sat, 15 Jan 2022 21:20:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:20:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:20:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:20:04: 7000000 INFO @ Sat, 15 Jan 2022 21:20:06: 1000000 INFO @ Sat, 15 Jan 2022 21:20:10: 8000000 INFO @ Sat, 15 Jan 2022 21:20:11: 2000000 INFO @ Sat, 15 Jan 2022 21:20:15: 9000000 INFO @ Sat, 15 Jan 2022 21:20:17: 3000000 INFO @ Sat, 15 Jan 2022 21:20:21: 10000000 INFO @ Sat, 15 Jan 2022 21:20:23: 4000000 INFO @ Sat, 15 Jan 2022 21:20:26: 11000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 21:20:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:20:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:20:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:20:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:20:32: 12000000 INFO @ Sat, 15 Jan 2022 21:20:34: 6000000 INFO @ Sat, 15 Jan 2022 21:20:35: 1000000 INFO @ Sat, 15 Jan 2022 21:20:37: 13000000 INFO @ Sat, 15 Jan 2022 21:20:39: 7000000 INFO @ Sat, 15 Jan 2022 21:20:41: 2000000 INFO @ Sat, 15 Jan 2022 21:20:42: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:20:42: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:20:42: #1 total tags in treatment: 13851359 INFO @ Sat, 15 Jan 2022 21:20:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:20:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:20:42: #1 tags after filtering in treatment: 13851359 INFO @ Sat, 15 Jan 2022 21:20:42: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:20:42: #1 finished! INFO @ Sat, 15 Jan 2022 21:20:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:20:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:20:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:20:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:20:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:20:45: 8000000 INFO @ Sat, 15 Jan 2022 21:20:47: 3000000 INFO @ Sat, 15 Jan 2022 21:20:50: 9000000 INFO @ Sat, 15 Jan 2022 21:20:52: 4000000 INFO @ Sat, 15 Jan 2022 21:20:55: 10000000 INFO @ Sat, 15 Jan 2022 21:20:57: 5000000 INFO @ Sat, 15 Jan 2022 21:21:01: 11000000 INFO @ Sat, 15 Jan 2022 21:21:03: 6000000 INFO @ Sat, 15 Jan 2022 21:21:06: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:21:08: 7000000 INFO @ Sat, 15 Jan 2022 21:21:12: 13000000 INFO @ Sat, 15 Jan 2022 21:21:14: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:21:17: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:21:17: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:21:17: #1 total tags in treatment: 13851359 INFO @ Sat, 15 Jan 2022 21:21:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:21:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:21:17: #1 tags after filtering in treatment: 13851359 INFO @ Sat, 15 Jan 2022 21:21:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:21:17: #1 finished! INFO @ Sat, 15 Jan 2022 21:21:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:21:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:21:18: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:21:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:21:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:21:19: 9000000 INFO @ Sat, 15 Jan 2022 21:21:24: 10000000 INFO @ Sat, 15 Jan 2022 21:21:29: 11000000 INFO @ Sat, 15 Jan 2022 21:21:34: 12000000 INFO @ Sat, 15 Jan 2022 21:21:39: 13000000 INFO @ Sat, 15 Jan 2022 21:21:43: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:21:43: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:21:43: #1 total tags in treatment: 13851359 INFO @ Sat, 15 Jan 2022 21:21:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:21:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:21:43: #1 tags after filtering in treatment: 13851359 INFO @ Sat, 15 Jan 2022 21:21:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:21:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:21:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:21:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:21:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:21:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:21:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833542/SRX8833542.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling