Job ID = 14520002 SRX = SRX8829451 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 41731518 spots for SRR12329245/SRR12329245.sra Written 41731518 spots for SRR12329245/SRR12329245.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:33 41731518 reads; of these: 41731518 (100.00%) were paired; of these: 3282247 (7.87%) aligned concordantly 0 times 28504251 (68.30%) aligned concordantly exactly 1 time 9945020 (23.83%) aligned concordantly >1 times ---- 3282247 pairs aligned concordantly 0 times; of these: 864134 (26.33%) aligned discordantly 1 time ---- 2418113 pairs aligned 0 times concordantly or discordantly; of these: 4836226 mates make up the pairs; of these: 3394185 (70.18%) aligned 0 times 779742 (16.12%) aligned exactly 1 time 662299 (13.69%) aligned >1 times 95.93% overall alignment rate Time searching: 00:25:33 Overall time: 00:25:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 12203672 / 39296785 = 0.3106 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:57:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:57:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:57:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:57:52: 1000000 INFO @ Sat, 15 Jan 2022 18:57:57: 2000000 INFO @ Sat, 15 Jan 2022 18:58:02: 3000000 INFO @ Sat, 15 Jan 2022 18:58:07: 4000000 INFO @ Sat, 15 Jan 2022 18:58:12: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:58:17: 6000000 INFO @ Sat, 15 Jan 2022 18:58:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:58:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:58:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:58:22: 7000000 INFO @ Sat, 15 Jan 2022 18:58:23: 1000000 INFO @ Sat, 15 Jan 2022 18:58:28: 8000000 INFO @ Sat, 15 Jan 2022 18:58:29: 2000000 INFO @ Sat, 15 Jan 2022 18:58:33: 9000000 INFO @ Sat, 15 Jan 2022 18:58:35: 3000000 INFO @ Sat, 15 Jan 2022 18:58:39: 10000000 INFO @ Sat, 15 Jan 2022 18:58:41: 4000000 INFO @ Sat, 15 Jan 2022 18:58:44: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:58:46: 5000000 INFO @ Sat, 15 Jan 2022 18:58:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:58:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:58:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:58:50: 12000000 INFO @ Sat, 15 Jan 2022 18:58:53: 6000000 INFO @ Sat, 15 Jan 2022 18:58:54: 1000000 INFO @ Sat, 15 Jan 2022 18:58:56: 13000000 INFO @ Sat, 15 Jan 2022 18:58:59: 7000000 INFO @ Sat, 15 Jan 2022 18:59:00: 2000000 INFO @ Sat, 15 Jan 2022 18:59:02: 14000000 INFO @ Sat, 15 Jan 2022 18:59:05: 8000000 INFO @ Sat, 15 Jan 2022 18:59:07: 3000000 INFO @ Sat, 15 Jan 2022 18:59:08: 15000000 INFO @ Sat, 15 Jan 2022 18:59:11: 9000000 INFO @ Sat, 15 Jan 2022 18:59:14: 4000000 INFO @ Sat, 15 Jan 2022 18:59:14: 16000000 INFO @ Sat, 15 Jan 2022 18:59:17: 10000000 INFO @ Sat, 15 Jan 2022 18:59:20: 17000000 INFO @ Sat, 15 Jan 2022 18:59:21: 5000000 INFO @ Sat, 15 Jan 2022 18:59:23: 11000000 INFO @ Sat, 15 Jan 2022 18:59:26: 18000000 INFO @ Sat, 15 Jan 2022 18:59:27: 6000000 INFO @ Sat, 15 Jan 2022 18:59:29: 12000000 INFO @ Sat, 15 Jan 2022 18:59:32: 19000000 INFO @ Sat, 15 Jan 2022 18:59:34: 7000000 INFO @ Sat, 15 Jan 2022 18:59:35: 13000000 INFO @ Sat, 15 Jan 2022 18:59:38: 20000000 INFO @ Sat, 15 Jan 2022 18:59:40: 8000000 INFO @ Sat, 15 Jan 2022 18:59:41: 14000000 INFO @ Sat, 15 Jan 2022 18:59:44: 21000000 INFO @ Sat, 15 Jan 2022 18:59:47: 9000000 INFO @ Sat, 15 Jan 2022 18:59:48: 15000000 INFO @ Sat, 15 Jan 2022 18:59:50: 22000000 INFO @ Sat, 15 Jan 2022 18:59:53: 10000000 INFO @ Sat, 15 Jan 2022 18:59:54: 16000000 INFO @ Sat, 15 Jan 2022 18:59:56: 23000000 INFO @ Sat, 15 Jan 2022 19:00:00: 17000000 INFO @ Sat, 15 Jan 2022 19:00:00: 11000000 INFO @ Sat, 15 Jan 2022 19:00:02: 24000000 INFO @ Sat, 15 Jan 2022 19:00:06: 18000000 INFO @ Sat, 15 Jan 2022 19:00:07: 12000000 INFO @ Sat, 15 Jan 2022 19:00:08: 25000000 INFO @ Sat, 15 Jan 2022 19:00:12: 19000000 INFO @ Sat, 15 Jan 2022 19:00:13: 13000000 INFO @ Sat, 15 Jan 2022 19:00:14: 26000000 INFO @ Sat, 15 Jan 2022 19:00:18: 20000000 INFO @ Sat, 15 Jan 2022 19:00:20: 14000000 INFO @ Sat, 15 Jan 2022 19:00:20: 27000000 INFO @ Sat, 15 Jan 2022 19:00:24: 21000000 INFO @ Sat, 15 Jan 2022 19:00:26: 28000000 INFO @ Sat, 15 Jan 2022 19:00:26: 15000000 INFO @ Sat, 15 Jan 2022 19:00:30: 22000000 INFO @ Sat, 15 Jan 2022 19:00:32: 29000000 INFO @ Sat, 15 Jan 2022 19:00:33: 16000000 INFO @ Sat, 15 Jan 2022 19:00:36: 23000000 INFO @ Sat, 15 Jan 2022 19:00:38: 30000000 INFO @ Sat, 15 Jan 2022 19:00:40: 17000000 INFO @ Sat, 15 Jan 2022 19:00:42: 24000000 INFO @ Sat, 15 Jan 2022 19:00:44: 31000000 INFO @ Sat, 15 Jan 2022 19:00:46: 18000000 INFO @ Sat, 15 Jan 2022 19:00:49: 25000000 INFO @ Sat, 15 Jan 2022 19:00:50: 32000000 INFO @ Sat, 15 Jan 2022 19:00:53: 19000000 INFO @ Sat, 15 Jan 2022 19:00:55: 26000000 INFO @ Sat, 15 Jan 2022 19:00:56: 33000000 INFO @ Sat, 15 Jan 2022 19:00:59: 20000000 INFO @ Sat, 15 Jan 2022 19:01:01: 27000000 INFO @ Sat, 15 Jan 2022 19:01:01: 34000000 INFO @ Sat, 15 Jan 2022 19:01:06: 21000000 INFO @ Sat, 15 Jan 2022 19:01:07: 28000000 INFO @ Sat, 15 Jan 2022 19:01:07: 35000000 INFO @ Sat, 15 Jan 2022 19:01:13: 29000000 INFO @ Sat, 15 Jan 2022 19:01:13: 22000000 INFO @ Sat, 15 Jan 2022 19:01:13: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:01:19: 30000000 INFO @ Sat, 15 Jan 2022 19:01:19: 37000000 INFO @ Sat, 15 Jan 2022 19:01:19: 23000000 INFO @ Sat, 15 Jan 2022 19:01:24: 31000000 INFO @ Sat, 15 Jan 2022 19:01:25: 38000000 INFO @ Sat, 15 Jan 2022 19:01:26: 24000000 INFO @ Sat, 15 Jan 2022 19:01:30: 32000000 INFO @ Sat, 15 Jan 2022 19:01:30: 39000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:01:33: 25000000 INFO @ Sat, 15 Jan 2022 19:01:36: 40000000 INFO @ Sat, 15 Jan 2022 19:01:36: 33000000 INFO @ Sat, 15 Jan 2022 19:01:39: 26000000 INFO @ Sat, 15 Jan 2022 19:01:42: 41000000 INFO @ Sat, 15 Jan 2022 19:01:42: 34000000 INFO @ Sat, 15 Jan 2022 19:01:46: 27000000 INFO @ Sat, 15 Jan 2022 19:01:48: 42000000 INFO @ Sat, 15 Jan 2022 19:01:48: 35000000 INFO @ Sat, 15 Jan 2022 19:01:53: 28000000 INFO @ Sat, 15 Jan 2022 19:01:53: 43000000 INFO @ Sat, 15 Jan 2022 19:01:54: 36000000 INFO @ Sat, 15 Jan 2022 19:01:59: 29000000 INFO @ Sat, 15 Jan 2022 19:01:59: 44000000 INFO @ Sat, 15 Jan 2022 19:02:00: 37000000 INFO @ Sat, 15 Jan 2022 19:02:05: 45000000 INFO @ Sat, 15 Jan 2022 19:02:06: 30000000 INFO @ Sat, 15 Jan 2022 19:02:06: 38000000 INFO @ Sat, 15 Jan 2022 19:02:11: 46000000 INFO @ Sat, 15 Jan 2022 19:02:12: 31000000 INFO @ Sat, 15 Jan 2022 19:02:12: 39000000 INFO @ Sat, 15 Jan 2022 19:02:17: 47000000 INFO @ Sat, 15 Jan 2022 19:02:18: 40000000 INFO @ Sat, 15 Jan 2022 19:02:18: 32000000 INFO @ Sat, 15 Jan 2022 19:02:22: 48000000 INFO @ Sat, 15 Jan 2022 19:02:24: 41000000 INFO @ Sat, 15 Jan 2022 19:02:25: 33000000 INFO @ Sat, 15 Jan 2022 19:02:28: 49000000 INFO @ Sat, 15 Jan 2022 19:02:29: 42000000 INFO @ Sat, 15 Jan 2022 19:02:31: 34000000 INFO @ Sat, 15 Jan 2022 19:02:34: 50000000 INFO @ Sat, 15 Jan 2022 19:02:35: 43000000 INFO @ Sat, 15 Jan 2022 19:02:38: 35000000 INFO @ Sat, 15 Jan 2022 19:02:39: 51000000 INFO @ Sat, 15 Jan 2022 19:02:41: 44000000 INFO @ Sat, 15 Jan 2022 19:02:44: 36000000 INFO @ Sat, 15 Jan 2022 19:02:45: 52000000 INFO @ Sat, 15 Jan 2022 19:02:46: 45000000 INFO @ Sat, 15 Jan 2022 19:02:50: 37000000 INFO @ Sat, 15 Jan 2022 19:02:51: 53000000 INFO @ Sat, 15 Jan 2022 19:02:52: 46000000 INFO @ Sat, 15 Jan 2022 19:02:56: 54000000 INFO @ Sat, 15 Jan 2022 19:02:56: 38000000 INFO @ Sat, 15 Jan 2022 19:02:58: 47000000 INFO @ Sat, 15 Jan 2022 19:03:02: 55000000 INFO @ Sat, 15 Jan 2022 19:03:02: 39000000 INFO @ Sat, 15 Jan 2022 19:03:03: 48000000 INFO @ Sat, 15 Jan 2022 19:03:06: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:03:06: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:03:06: #1 total tags in treatment: 26303127 INFO @ Sat, 15 Jan 2022 19:03:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:03:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:03:06: #1 tags after filtering in treatment: 8577116 INFO @ Sat, 15 Jan 2022 19:03:06: #1 Redundant rate of treatment: 0.67 INFO @ Sat, 15 Jan 2022 19:03:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:03:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:03:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:03:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:03:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:03:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:03:08: 40000000 INFO @ Sat, 15 Jan 2022 19:03:09: 49000000 INFO @ Sat, 15 Jan 2022 19:03:14: 50000000 INFO @ Sat, 15 Jan 2022 19:03:15: 41000000 INFO @ Sat, 15 Jan 2022 19:03:19: 51000000 INFO @ Sat, 15 Jan 2022 19:03:21: 42000000 INFO @ Sat, 15 Jan 2022 19:03:25: 52000000 INFO @ Sat, 15 Jan 2022 19:03:27: 43000000 INFO @ Sat, 15 Jan 2022 19:03:30: 53000000 INFO @ Sat, 15 Jan 2022 19:03:33: 44000000 INFO @ Sat, 15 Jan 2022 19:03:36: 54000000 INFO @ Sat, 15 Jan 2022 19:03:39: 45000000 INFO @ Sat, 15 Jan 2022 19:03:41: 55000000 INFO @ Sat, 15 Jan 2022 19:03:45: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:03:45: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:03:45: #1 total tags in treatment: 26303127 INFO @ Sat, 15 Jan 2022 19:03:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:03:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:03:45: #1 tags after filtering in treatment: 8577116 INFO @ Sat, 15 Jan 2022 19:03:45: #1 Redundant rate of treatment: 0.67 INFO @ Sat, 15 Jan 2022 19:03:45: #1 finished! INFO @ Sat, 15 Jan 2022 19:03:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:03:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:03:45: 46000000 INFO @ Sat, 15 Jan 2022 19:03:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:03:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:03:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:03:51: 47000000 INFO @ Sat, 15 Jan 2022 19:03:56: 48000000 INFO @ Sat, 15 Jan 2022 19:04:02: 49000000 INFO @ Sat, 15 Jan 2022 19:04:07: 50000000 INFO @ Sat, 15 Jan 2022 19:04:13: 51000000 INFO @ Sat, 15 Jan 2022 19:04:19: 52000000 INFO @ Sat, 15 Jan 2022 19:04:24: 53000000 INFO @ Sat, 15 Jan 2022 19:04:30: 54000000 INFO @ Sat, 15 Jan 2022 19:04:35: 55000000 INFO @ Sat, 15 Jan 2022 19:04:39: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 19:04:39: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 19:04:39: #1 total tags in treatment: 26303127 INFO @ Sat, 15 Jan 2022 19:04:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:04:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:04:39: #1 tags after filtering in treatment: 8577116 INFO @ Sat, 15 Jan 2022 19:04:39: #1 Redundant rate of treatment: 0.67 INFO @ Sat, 15 Jan 2022 19:04:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:04:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:04:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:04:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:04:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:04:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8829451/SRX8829451.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling