Job ID = 11193197 sra ファイルのダウンロード中... Completed: 172351K bytes transferred in 6 seconds (213278K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 3946497 spots for /home/okishinya/chipatlas/results/sacCer3/SRX876286/SRR1802389.sra Written 3946497 spots for /home/okishinya/chipatlas/results/sacCer3/SRX876286/SRR1802389.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:54 3946497 reads; of these: 3946497 (100.00%) were paired; of these: 3006151 (76.17%) aligned concordantly 0 times 785356 (19.90%) aligned concordantly exactly 1 time 154990 (3.93%) aligned concordantly >1 times ---- 3006151 pairs aligned concordantly 0 times; of these: 21362 (0.71%) aligned discordantly 1 time ---- 2984789 pairs aligned 0 times concordantly or discordantly; of these: 5969578 mates make up the pairs; of these: 5912007 (99.04%) aligned 0 times 37959 (0.64%) aligned exactly 1 time 19612 (0.33%) aligned >1 times 25.10% overall alignment rate Time searching: 00:00:54 Overall time: 00:00:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 746570 / 955951 = 0.7810 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 11:08:04: # Command line: callpeak -t SRX876286.bam -f BAM -g 12100000 -n SRX876286.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX876286.05 # format = BAM # ChIP-seq file = ['SRX876286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:08:04: # Command line: callpeak -t SRX876286.bam -f BAM -g 12100000 -n SRX876286.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX876286.20 # format = BAM # ChIP-seq file = ['SRX876286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:08:04: # Command line: callpeak -t SRX876286.bam -f BAM -g 12100000 -n SRX876286.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX876286.10 # format = BAM # ChIP-seq file = ['SRX876286.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:08:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:08:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:08:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:08:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:08:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:08:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:08:07: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:08:07: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:08:07: #1 total tags in treatment: 208053 INFO @ Sat, 15 Sep 2018 11:08:07: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:08:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:08:07: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:08:07: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:08:07: #1 total tags in treatment: 208053 INFO @ Sat, 15 Sep 2018 11:08:07: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:08:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:08:07: #1 tags after filtering in treatment: 189284 INFO @ Sat, 15 Sep 2018 11:08:07: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 15 Sep 2018 11:08:07: #1 finished! INFO @ Sat, 15 Sep 2018 11:08:07: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:08:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:08:07: #1 tags after filtering in treatment: 189284 INFO @ Sat, 15 Sep 2018 11:08:07: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 15 Sep 2018 11:08:07: #1 finished! INFO @ Sat, 15 Sep 2018 11:08:07: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:08:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:08:07: #2 number of paired peaks: 153 WARNING @ Sat, 15 Sep 2018 11:08:07: Fewer paired peaks (153) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 153 pairs to build model! INFO @ Sat, 15 Sep 2018 11:08:07: start model_add_line... INFO @ Sat, 15 Sep 2018 11:08:07: start X-correlation... INFO @ Sat, 15 Sep 2018 11:08:07: #2 number of paired peaks: 153 WARNING @ Sat, 15 Sep 2018 11:08:07: Fewer paired peaks (153) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 153 pairs to build model! INFO @ Sat, 15 Sep 2018 11:08:07: start model_add_line... INFO @ Sat, 15 Sep 2018 11:08:07: start X-correlation... INFO @ Sat, 15 Sep 2018 11:08:07: end of X-cor INFO @ Sat, 15 Sep 2018 11:08:07: #2 finished! INFO @ Sat, 15 Sep 2018 11:08:07: #2 predicted fragment length is 89 bps INFO @ Sat, 15 Sep 2018 11:08:07: #2 alternative fragment length(s) may be 67,89,481,582 bps INFO @ Sat, 15 Sep 2018 11:08:07: #2.2 Generate R script for model : SRX876286.05_model.r INFO @ Sat, 15 Sep 2018 11:08:07: end of X-cor INFO @ Sat, 15 Sep 2018 11:08:07: #2 finished! INFO @ Sat, 15 Sep 2018 11:08:07: #2 predicted fragment length is 89 bps INFO @ Sat, 15 Sep 2018 11:08:07: #2 alternative fragment length(s) may be 67,89,481,582 bps INFO @ Sat, 15 Sep 2018 11:08:07: #2.2 Generate R script for model : SRX876286.20_model.r INFO @ Sat, 15 Sep 2018 11:08:07: #3 Call peaks... INFO @ Sat, 15 Sep 2018 11:08:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 11:08:07: #3 Call peaks... INFO @ Sat, 15 Sep 2018 11:08:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 11:08:07: #1 tag size is determined as 40 bps INFO @ Sat, 15 Sep 2018 11:08:07: #1 tag size = 40 INFO @ Sat, 15 Sep 2018 11:08:07: #1 total tags in treatment: 208053 INFO @ Sat, 15 Sep 2018 11:08:07: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:08:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:08:07: #1 tags after filtering in treatment: 189284 INFO @ Sat, 15 Sep 2018 11:08:07: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 15 Sep 2018 11:08:07: #1 finished! INFO @ Sat, 15 Sep 2018 11:08:07: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:08:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:08:07: #2 number of paired peaks: 153 WARNING @ Sat, 15 Sep 2018 11:08:07: Fewer paired peaks (153) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 153 pairs to build model! INFO @ Sat, 15 Sep 2018 11:08:07: start model_add_line... INFO @ Sat, 15 Sep 2018 11:08:07: start X-correlation... INFO @ Sat, 15 Sep 2018 11:08:07: end of X-cor INFO @ Sat, 15 Sep 2018 11:08:07: #2 finished! INFO @ Sat, 15 Sep 2018 11:08:07: #2 predicted fragment length is 89 bps INFO @ Sat, 15 Sep 2018 11:08:07: #2 alternative fragment length(s) may be 67,89,481,582 bps INFO @ Sat, 15 Sep 2018 11:08:07: #2.2 Generate R script for model : SRX876286.10_model.r INFO @ Sat, 15 Sep 2018 11:08:07: #3 Call peaks... INFO @ Sat, 15 Sep 2018 11:08:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 11:08:07: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 11:08:07: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 11:08:07: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 11:08:07: #4 Write output xls file... SRX876286.05_peaks.xls INFO @ Sat, 15 Sep 2018 11:08:07: #4 Write peak in narrowPeak format file... SRX876286.05_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 11:08:07: #4 Write summits bed file... SRX876286.05_summits.bed INFO @ Sat, 15 Sep 2018 11:08:07: Done! INFO @ Sat, 15 Sep 2018 11:08:07: #4 Write output xls file... SRX876286.20_peaks.xls INFO @ Sat, 15 Sep 2018 11:08:07: #4 Write peak in narrowPeak format file... SRX876286.20_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 11:08:07: #4 Write summits bed file... SRX876286.20_summits.bed INFO @ Sat, 15 Sep 2018 11:08:07: Done! pass1 - making usageList (4 chroms): 1 millis pass2 - checking and writing primary data (5 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:08:08: #4 Write output xls file... SRX876286.10_peaks.xls INFO @ Sat, 15 Sep 2018 11:08:08: #4 Write peak in narrowPeak format file... SRX876286.10_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 11:08:08: #4 Write summits bed file... SRX876286.10_summits.bed INFO @ Sat, 15 Sep 2018 11:08:08: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (42 records, 4 fields): 2 millis CompletedMACS2peakCalling pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (119 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。