Job ID = 14520294 SRX = SRX8421787 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 21896232 spots for SRR11872080/SRR11872080.sra Written 21896232 spots for SRR11872080/SRR11872080.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:07 21896232 reads; of these: 21896232 (100.00%) were unpaired; of these: 2410513 (11.01%) aligned 0 times 16895796 (77.16%) aligned exactly 1 time 2589923 (11.83%) aligned >1 times 88.99% overall alignment rate Time searching: 00:07:07 Overall time: 00:07:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10177809 / 19485719 = 0.5223 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:06:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:06:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:06:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:06:55: 1000000 INFO @ Sat, 15 Jan 2022 19:07:02: 2000000 INFO @ Sat, 15 Jan 2022 19:07:10: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:07:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:07:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:07:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:07:17: 4000000 INFO @ Sat, 15 Jan 2022 19:07:27: 5000000 INFO @ Sat, 15 Jan 2022 19:07:28: 1000000 INFO @ Sat, 15 Jan 2022 19:07:36: 6000000 INFO @ Sat, 15 Jan 2022 19:07:39: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:07:46: 7000000 INFO @ Sat, 15 Jan 2022 19:07:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:07:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:07:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:07:50: 3000000 INFO @ Sat, 15 Jan 2022 19:07:55: 8000000 INFO @ Sat, 15 Jan 2022 19:07:57: 1000000 INFO @ Sat, 15 Jan 2022 19:08:01: 4000000 INFO @ Sat, 15 Jan 2022 19:08:05: 9000000 INFO @ Sat, 15 Jan 2022 19:08:06: 2000000 INFO @ Sat, 15 Jan 2022 19:08:08: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:08:08: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:08:08: #1 total tags in treatment: 9307910 INFO @ Sat, 15 Jan 2022 19:08:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:08:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:08:08: #1 tags after filtering in treatment: 9307910 INFO @ Sat, 15 Jan 2022 19:08:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:08:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:08:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:08:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:08:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:08:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:08:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:08:12: 5000000 INFO @ Sat, 15 Jan 2022 19:08:16: 3000000 INFO @ Sat, 15 Jan 2022 19:08:23: 6000000 INFO @ Sat, 15 Jan 2022 19:08:25: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:08:34: 7000000 INFO @ Sat, 15 Jan 2022 19:08:35: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:08:44: 8000000 INFO @ Sat, 15 Jan 2022 19:08:44: 6000000 INFO @ Sat, 15 Jan 2022 19:08:54: 7000000 INFO @ Sat, 15 Jan 2022 19:08:55: 9000000 INFO @ Sat, 15 Jan 2022 19:08:58: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:08:58: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:08:58: #1 total tags in treatment: 9307910 INFO @ Sat, 15 Jan 2022 19:08:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:08:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:08:58: #1 tags after filtering in treatment: 9307910 INFO @ Sat, 15 Jan 2022 19:08:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:08:58: #1 finished! INFO @ Sat, 15 Jan 2022 19:08:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:08:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:08:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:08:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:08:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:09:03: 8000000 INFO @ Sat, 15 Jan 2022 19:09:11: 9000000 INFO @ Sat, 15 Jan 2022 19:09:14: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:09:14: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:09:14: #1 total tags in treatment: 9307910 INFO @ Sat, 15 Jan 2022 19:09:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:09:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:09:14: #1 tags after filtering in treatment: 9307910 INFO @ Sat, 15 Jan 2022 19:09:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:09:14: #1 finished! INFO @ Sat, 15 Jan 2022 19:09:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:09:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:09:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:09:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:09:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8421787/SRX8421787.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling