Job ID = 14520745 SRX = SRX8245506 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20258564 spots for SRR11684717/SRR11684717.sra Written 20258564 spots for SRR11684717/SRR11684717.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:53 20258564 reads; of these: 20258564 (100.00%) were paired; of these: 8260421 (40.77%) aligned concordantly 0 times 10259578 (50.64%) aligned concordantly exactly 1 time 1738565 (8.58%) aligned concordantly >1 times ---- 8260421 pairs aligned concordantly 0 times; of these: 158776 (1.92%) aligned discordantly 1 time ---- 8101645 pairs aligned 0 times concordantly or discordantly; of these: 16203290 mates make up the pairs; of these: 11936052 (73.66%) aligned 0 times 3516567 (21.70%) aligned exactly 1 time 750671 (4.63%) aligned >1 times 70.54% overall alignment rate Time searching: 00:10:53 Overall time: 00:10:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2355422 / 12154464 = 0.1938 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:06:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:06:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:06:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:06:16: 1000000 INFO @ Sat, 15 Jan 2022 20:06:21: 2000000 INFO @ Sat, 15 Jan 2022 20:06:27: 3000000 INFO @ Sat, 15 Jan 2022 20:06:32: 4000000 INFO @ Sat, 15 Jan 2022 20:06:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:06:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:06:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:06:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:06:42: 6000000 INFO @ Sat, 15 Jan 2022 20:06:48: 1000000 INFO @ Sat, 15 Jan 2022 20:06:48: 7000000 INFO @ Sat, 15 Jan 2022 20:06:55: 8000000 INFO @ Sat, 15 Jan 2022 20:06:55: 2000000 INFO @ Sat, 15 Jan 2022 20:07:01: 9000000 INFO @ Sat, 15 Jan 2022 20:07:03: 3000000 INFO @ Sat, 15 Jan 2022 20:07:07: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:07:10: 4000000 INFO @ Sat, 15 Jan 2022 20:07:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:07:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:07:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:07:14: 11000000 INFO @ Sat, 15 Jan 2022 20:07:17: 5000000 INFO @ Sat, 15 Jan 2022 20:07:18: 1000000 INFO @ Sat, 15 Jan 2022 20:07:20: 12000000 INFO @ Sat, 15 Jan 2022 20:07:24: 6000000 INFO @ Sat, 15 Jan 2022 20:07:25: 2000000 INFO @ Sat, 15 Jan 2022 20:07:26: 13000000 INFO @ Sat, 15 Jan 2022 20:07:31: 7000000 INFO @ Sat, 15 Jan 2022 20:07:32: 3000000 INFO @ Sat, 15 Jan 2022 20:07:33: 14000000 INFO @ Sat, 15 Jan 2022 20:07:38: 8000000 INFO @ Sat, 15 Jan 2022 20:07:39: 4000000 INFO @ Sat, 15 Jan 2022 20:07:39: 15000000 INFO @ Sat, 15 Jan 2022 20:07:45: 9000000 INFO @ Sat, 15 Jan 2022 20:07:45: 16000000 INFO @ Sat, 15 Jan 2022 20:07:45: 5000000 INFO @ Sat, 15 Jan 2022 20:07:52: 10000000 INFO @ Sat, 15 Jan 2022 20:07:52: 17000000 INFO @ Sat, 15 Jan 2022 20:07:52: 6000000 INFO @ Sat, 15 Jan 2022 20:07:58: 18000000 INFO @ Sat, 15 Jan 2022 20:07:58: 11000000 INFO @ Sat, 15 Jan 2022 20:07:59: 7000000 INFO @ Sat, 15 Jan 2022 20:08:04: 19000000 INFO @ Sat, 15 Jan 2022 20:08:05: 12000000 INFO @ Sat, 15 Jan 2022 20:08:06: 8000000 INFO @ Sat, 15 Jan 2022 20:08:11: 20000000 INFO @ Sat, 15 Jan 2022 20:08:12: 9000000 INFO @ Sat, 15 Jan 2022 20:08:12: 13000000 INFO @ Sat, 15 Jan 2022 20:08:17: 21000000 INFO @ Sat, 15 Jan 2022 20:08:19: 10000000 INFO @ Sat, 15 Jan 2022 20:08:20: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:08:23: 22000000 INFO @ Sat, 15 Jan 2022 20:08:26: 11000000 INFO @ Sat, 15 Jan 2022 20:08:27: 15000000 INFO @ Sat, 15 Jan 2022 20:08:30: 23000000 INFO @ Sat, 15 Jan 2022 20:08:33: 12000000 INFO @ Sat, 15 Jan 2022 20:08:34: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:08:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:08:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:08:35: #1 total tags in treatment: 9659971 INFO @ Sat, 15 Jan 2022 20:08:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:08:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:08:36: #1 tags after filtering in treatment: 6817206 INFO @ Sat, 15 Jan 2022 20:08:36: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:08:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:08:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:08:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:08:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:08:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:08:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:08:39: 13000000 INFO @ Sat, 15 Jan 2022 20:08:41: 17000000 INFO @ Sat, 15 Jan 2022 20:08:45: 14000000 INFO @ Sat, 15 Jan 2022 20:08:48: 18000000 INFO @ Sat, 15 Jan 2022 20:08:52: 15000000 INFO @ Sat, 15 Jan 2022 20:08:55: 19000000 INFO @ Sat, 15 Jan 2022 20:08:58: 16000000 INFO @ Sat, 15 Jan 2022 20:09:02: 20000000 INFO @ Sat, 15 Jan 2022 20:09:04: 17000000 INFO @ Sat, 15 Jan 2022 20:09:09: 21000000 INFO @ Sat, 15 Jan 2022 20:09:10: 18000000 INFO @ Sat, 15 Jan 2022 20:09:16: 22000000 INFO @ Sat, 15 Jan 2022 20:09:16: 19000000 INFO @ Sat, 15 Jan 2022 20:09:23: 20000000 INFO @ Sat, 15 Jan 2022 20:09:23: 23000000 INFO @ Sat, 15 Jan 2022 20:09:29: 21000000 INFO @ Sat, 15 Jan 2022 20:09:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:09:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:09:29: #1 total tags in treatment: 9659971 INFO @ Sat, 15 Jan 2022 20:09:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:30: #1 tags after filtering in treatment: 6817206 INFO @ Sat, 15 Jan 2022 20:09:30: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:09:30: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:09:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:09:35: 22000000 INFO @ Sat, 15 Jan 2022 20:09:41: 23000000 INFO @ Sat, 15 Jan 2022 20:09:45: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:09:45: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:09:45: #1 total tags in treatment: 9659971 INFO @ Sat, 15 Jan 2022 20:09:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:46: #1 tags after filtering in treatment: 6817206 INFO @ Sat, 15 Jan 2022 20:09:46: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 20:09:46: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:09:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8245506/SRX8245506.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling